Large‑scale transcriptomic and genomic analyses reveal a novel functional gene SERPINB6 for chicken carcass traits

Background Carcass traits are crucial indicators of meat production efficiency.However,the molecular regulatory mechanisms associated with these traits remain unclear.Results In this study,we conducted comprehensive transcriptomic and genomic analyses on 399 Tiannong partridge chickens to identify key genes and variants associated with carcass traits and to elucidate the underlying regulatory mechanisms.Based on association analyses with the elastic net(EN)model,we identified 12 candidate genes(AMY1A,AP3B2,CEBPG,EEF2,EIF4EBP1,FGFR1,FOXD3,GOLM1,LOC107052698,PABPC1,SERPINB6 and TBC1D16)for 4 carcass-related traits,namely live weight,dressed weight,eviscerated weight,and breast muscle weight.SERPINB6 was identified as the only overlapping gene by 3 analyses,EN model analysis,weighted gene co-expression network analysis and differential expression analysis.Cell-level experiments confirmed that SERPINB6 promotes the proliferation of chicken DF1 cells and primary myoblasts.Further expression genome-wide association study and association analysis indicated that rs317934171 is the critical site that enhances SERPINB6 expression.Furthermore,a dual-luciferase reporter assay proved that gga-miR-1615 targets the 3′UTR of SERPINB6.Conclusions Collectively,our findings reveal that SERPINB6 serves as a novel gene for chicken carcass traits by promoting fibroblast and myoblast proliferation.Additionally,the downstream variant rs317934171 regulates SERPINB6 expression.These results identify a new target gene and molecular marker for the molecular mechanisms of chicken carcass traits.

Strategies to improve genomic predictions for 35 duck carcass traits in an F2 population

Background Carcass traits are crucial for broiler ducks,but carcass traits can only be measured postmortem.Genomic selection(GS)is an effective approach in animal breeding to improve selection and reduce costs.However,the performance of genomic prediction in duck carcass traits remains largely unknown.Results In this study,we estimated the genetic parameters,performed GS using different models and marker densi-ties,and compared the estimation performance between GS and conventional BLUP on 35 carcass traits in an F2 population of ducks.Most of the cut weight traits and intestine length traits were estimated to be high and moder-ate heritabilities,respectively,while the heritabilities of percentage slaughter traits were dynamic.The reliability of genome prediction using GBLUP increased by an average of 0.06 compared to the conventional BLUP method.The Permutation studies revealed that 50K markers had achieved ideal prediction reliability,while 3K markers still achieved 90.7%predictive capability would further reduce the cost for duck carcass traits.The genomic relationship matrix nor-malized by our true variance method instead of the widely used 2pi(1-pi)could achieve an increase in prediction reliability in most traits.We detected most of the bayesian models had a better performance,especially for BayesN.Compared to GBLUP,BayesN can further improve the predictive reliability with an average of 0.06 for duck carcass traits.Conclusion This study demonstrates genomic selection for duck carcass traits is promising.The genomic prediction can be further improved by modifying the genomic relationship matrix using our proposed true variance method and several Bayesian models.Permutation study provides a theoretical basis for the fact that low-density arrays can be used to reduce genotype costs in duck genome selection.

Expression of GHR, IGF-I, IGF-IR and IGFBP-3 Genes in Leg Muscles of Taihu Goose and Wanxi White Goose and Correlation between Their Expression and Carcass Traits

[Objective] This study aimed to explore the effects of IGFs system on the growth of goose skeletal muscles. [Method] Quantitative fluorescence PCR technique was adopted to study the variety- and gender-specificity in the expression of GHR, IGF-I, IGF-IR and IGFBP-3 genes in leg muscles of 70-day-old Taihu goose and Wanxi white goose, and the correlation between their expression and carcass traits was also investigated. [Resull] There was no variety difference in the expression of GHR, IGF-t, IGF-IR and IGFBP-3 genes in leg muscles of Taihu goose and Wanxi white goose, but there were significant variety differences in the body weight and leg muscle weight. There were no gender difference in the body weight, leg muscle weight and the rate of leg muscles; except IGF-I mRNA level that was significantly higher in male Taihu goose than in female ones（P=0.032）, there was no gender dif- ference in the expression of other three genes. Among the four tested genes, only IGFBP-3 mRNA exhibited an extremely significantly positively correlation with the rate of leg muscles, suggesting that IGFs may play a role in regulating the growth of leg muscles via IGFBP-3 system in 70-day-old goose. [Conclusion] This study provides theoretical basis for research in the skeletal growth and development.

Correlation Analysis on Single Nucleotide Polymorphism of CAPN1 Gene and Meat Quality and Carcass Traits in Chickens

The selection of meat quality has received considerable focus in chicken breeding. This study was aimed at investigating the effect of CAPN1 gene on meat quality traits in chicken populations. Primer pairs for 3 UTR in CAPN1 were designed from database of chicken genomic sequence. Polymorphisms were detected using PCR-SSCP and DNA sequencing. A mutation at position 9 950 nt （G/A, locus A） was found among individuals in each population. The allele and genotype frequencies significantly differed among eight lines with higher frequencies of allele A2 and genotype AIA2 （P〈0.01）. The least square analysis showed that there was significant difference （P 〈 0.05） in muscle fiber density and some carcass traits among genotypes and that the breast muscle fiber density （BFD） of birds ofAIA1 genotype was significantly higher （P〈0.05） than that of birds ofA2A2 genotype. It was concluded that the CAPN1 gene was the major gene affecting the muscle fiber traits of chicken or was linked with the major gene. These results were useful for studying the molecular mechanism that influences meat traits and were used as the base of molecular-assisted selection to meat quality traits. So, this site may be a potential marker affecting the muscle traits of chickens.

Effects of Hybridizing Berkshire Boar with Landrace × Yorkshire Sow on the Carcass Traits and Meat Quality of Hybrid Progeny

[ Objective] The research aimed to provide theoretical basis for further popularization and application of Berkshire Pig in Southeast Guizhou. [ Method] The carcass traits and meat quality of Berkshire（B） × Landrace（L） × Yorkshire（Y）, Duroc（D） × Landrace（L） x Yorkshire（Y） and Lanclrace （L） xYorkshire（Y） were determined by slaughter test. And the application effect of Berkshire in the minority areas was analyzed. [Result] From the carcass traits, the hybridized pig of Berkshire（B） x Landrace（L） ×Yorkshire（Y） had higher slaughter rate and lower lean meat percentage, having extremely significant difference with Landrace （L） × Yorkshire （Y） and significant difference with Durec （D） × Landrace （L） × Yorkshire（Y）. The meat indices of Berkshire（B） × Landrace（L） ×Yorkshire（Y） were better than that of Landrace （L） ×Yorkshire（Y） and Duroc （D）× Landrace（L） ×Yorkshire（Y）. The eye muscle area of Berkshire（B） ×Landrace（L） xYorkshire（Y） had extremely significant difference with Landrace （L） × Yorkshire（Y） and significant difference with Duroc（D） × Landrace（L） × Yorkshire（Y）. The meat color, marbling and cocking percentage and other meat indices were similar with that of Jianhe White Xiang Pig, Nayong Nuogu Pig and Cola Pig. [ Conclusion] Berkshire（B） × Landrace（L） xYorkshire（Y） was more suiTab, for residents in the minority mountain areas, so it was worthy to be popularized.

Association of Single Nucleotide Polymorphisms in Exon 3 of Porcine LMCD1 Gene with Meat Quality and Carcass Traits

LIM domain proteins are found to be important regulators in cell growth, cell fate determination, cell differentiation, and remodelling of the cell cytoskeleton by their interaction with some structural proteins, kinases, transcriptional regulators, etc. The presence of LIM domains in LMCD1 gene implies it may be involved in skeletal muscle protein-protein interactions. This study was to investigate polymorphisms of LIM and cysteine-rich domain 1 （LMCD1） gene and its effect on meat quality and carcass traits in pig. The polymorphism （G294A） in exon 3 region of porcine LMCD1 gene, which is synonymous mutation, was genotyped in the population of 178 F, pigs of a Large White × Meishan resource family. Statistical results indicated the distribution of allele G （with a A → G mutation） and A （without mutation）. Analysis of variance showed that the polymorphism of LMCD1 gene was associated with variation in several carcass traits of interest for pig breeding. Some carcass traits and meat quality traits are close to significance by association. An analysis of more animals is necessary to analyze the polymorphisms in exon 3 of porcine LMCD1 gene if it was selected as a marker for the pig carcass traits.

Growth Performances and Carcass Traits of Different Duck Breeds

[ Objective] To investigate the genetic differences of growth performances and camass traits between different duck breeds and provide a reference for breeding fine ducks. [Method] Three kinds of healthy ducks including Cherry Valley duck, White Muscow duck and Jingjiang duck were selected as experimental animals. After they were fed under the same raising condition for 8 weeks, the body weight and meat traits were determined. [ Result] The growth performance had significant differences between three duck breeds （P 〈 0.05）; the Cherry Valley duck had the fastest growth, the Jingjiang duck grew most slowly, and the White Muscovy duck started to grow rapidly at the age of 4 weeks. The carcass weight, eviscerated weight, lean meat percentage, breast muscle rate, subcutaneous fat percentage and abdominal fat percentage of Cherry Valley duck were higher than those of other two duck breeds （ P 〈 0.05）, and the abdominal fat percentage of Jingjiang duck was 0. The eviscerated rate, breast muscle rate and dressing percentage of Cherry Valley duck were significantly lower than those of Jingjiang duck （ P 〈 0.05）. The carcass traits had differences between male and female ducks. [ Conclusion] The growth performances and carcass traits are different in these three duck breeds.

Effect of Graded Levels of Wheat Distillers Grains with Solubles on Nutrient Digestibility,Performance and Carcass Traits of Growing-Finishing Pigs Fed Diets Based on Wheat and Field Peas

This trial was conducted to determine the effects of graded levels of wheat distillers dried grains with solubles （DDGS） on nutrient digestibility, performance and carcass characteristics of pigs （21.8 to 112.3 kg） fed diets based on wheat and field peas. Forty crossbred pigs were assigned on the basis of sex and weight to one of five dietary treatments in a 5 x 2 （treatment x sex） factorial arrangement. The experimental diets were based on wheat and field peas and were formulated to contain 0, 4.86%, 9.71%, 14.57% or 19.42% wheat DDGS during the growing period and 0, 4.01%, 8.05%, 12.10% and 16.14% wheat DDGS during the finishing period. At the highest levels, ~vheat DDGS supplied 100% of the supplementary protein. The digestibility coefficient for gross energy showed a tendency towards a linear decline （P 〈 0.08 ） as the level of wheat DDGS in the diet increased while digestibility coefficients for dry matter and crude protein were unaffected （P 〉 0. 05 ） by inclusion level of wheat DDGS. During the growing period （21.8 to 60.8 kg）, weight gain of pigs fed wheat DDGS declined （P〈0.01） linearly. The impairment in growth appeared to be associated with a decrease in feed in- take which also declined linearly （P 〈 0.01 ） with increasing inclusion level of wheat DDGS. Feed conversion was unaffected by level of wheat DDGS. During the finishing period （60.8 to 112.3 kg）, and over the entire experimental period （21.8 to 112.3 kg ）, weight gain and feed conversion declined linearly as the dietary inclusion level of wheat DDGS increased. Carcass traits were generally unaffected by the inclusion of wheat DDGS with the exception of a trend towards a linear （P = 0.10） reduction in lean yield and a linear increase （ P = 0.09） in loin fat as the level of wheat DDGS in the diet increased. Overall, these results suggest that wheat DDGS are inferior to field peas as a supplementary protein source for use in swine rations.

Carcass Traits of the Malagasy Zebu ＂Bos taurus indicus＂ （Linnaeus, 1758）

Improving Malagasy zebu production in the face of booming Malagasy population and regression of the animal number, this study was initiated to put forth some Malagasy zebu carcass weight performance and dressing percentages following slaughter. Measurements concerned live weight before slaughtering, carcass weights, quarters and carcass halves, left and right and the fiffla quarter （blood, leather, intestine, lung, heart, liver, kidneys, spleen and rumen with its content） weights on 544 individuals without distinction （age nor sex）. Descriptive statistical analysis was undertaken to qualify and compare the various weights. It results high proportion females （66%）, smaller size （live weight less than 250 kg, 60%） and 5 live weight classes. Live weight was different in the 2 sexes starting class 3 （live weight 〉 250 kg） （p = 0.0027）. Males fore quarters were heavier than females （p 〈 1‰）. Carcass dressing percentage increased according to live weight class, whereas, 5th quarter yield presented a regressive reduction with increasing weight class. Survey shows an average dressing percentage of 46.20% with an average 5th quarter yield of 37.43%. Malagasy zebus are light weighted animals compared to the African zebus. Precocious slaughtering （before 250 kg live weight） doesn＇t allow a good meat yield.

Carcass Traits, Meat Yield and Primal Meat Cuts from Arsi, Harar, Ogaden and F1 Jersey*Horro Crossbred Bulls Fed Corn Silage Based Similar Finishing Diet

This study was conducted with the objective to determine carcass traits, meat yield, and primal meat cuts of Arsi, Harar, Jersey*Horro crossbred, and Ogaden cattle breeds at Haramaya University, Ethiopia. A total of 12 bulls of four cattle breeds (3 Arsi, 3 Harar, 3 F1 Jersey*Horro crossbred and 3 Ogaden) with almost similar ages were randomly assigned to four treatments in a completely randomised design (CRD). Data on carcass traits, meat yield, and primal meat cuts were analyzed using the General Linear Model (GLM) of the Statistical Analysis Software (SAS) 9.4 version. The overall averages of live body weight, hot carcass weight, chilled carcass weight, dressing percentages based on hot carcass weight, and rib eye area of experimental cattle breeds were 215.58 kg, 102.93 kg, 99.56 kg, 47.61%, and 8.13 inch2, respectively. The hot carcass weight and chilled carcass weight of the Ogaden (136.57;133.30 kg, resp.) breed were higher (p < 0.01) compared to other experimental cattle breeds. Dressing percentages based on hot carcass weight were higher (p < 0.05) for the Ogaden (49.61%) and Arsi (49.82%) cattle breeds compared to Harar and Jersey*Horro crossbred (45.73%, 45.27%, resp.) cattle breeds. The average meat yield and proportion of meat yield of cattle breeds were 77.52 kg and 77.46%, respectively. With a linear regression coefficient of prediction (R2) ranging from 52.26% to 93.58%, primal meat cuts significantly (p dicted meat yield. In conclusion, the breed of cattle had a significant (p 0.05) influence on live body weight, hot and chilled carcass weight, dressing percentage, rib eye area, subcutaneous fat thickness, meat yield, and the weights of most primal meat cuts. The Ogaden cattle breed had a higher and better meat yield, carcass traits, and most primal meat cuts compared to other experimental cattle breeds. Furthermore, the inclusion of corn silage in the diet of fattening bulls improved the carcass and meat yield. Therefore, the performance of Ogaden cattle compared to other and previous studies suggests the possibility of using this breed for export purposes in addition to Borana and Harar cattle breeds in the future.

Genome-Wide Association Study for Certain Carcass Traits and Organ Weights in a Large White×Minzhu Intercross Porcine Population

Porcine carcass traits and organ weights have important economic roles in the swine industry. A total of 576 animals from a Large White×Minzhu intercross population were genotyped using the Illumina PorcineSNP60K Beadchip and were phenotyped for 10 traits, speciifcally, backfat thickness （6-7 libs）, carcass length, carcass weight, foot weight, head weight, heart weight, leaf fat weight, liver weight, lung weight and slaughter body weight. The genome-wide association study （GWAS） was assessed by Genome Wide Rapid Association using the mixed model and regression-genomic control approach. A total of 31 single nucleotide polymorphisms （SNPs） （with the most signiifcant SNP being MARC0033464, P value=6.80×10-13） were located in a 9.76-Mb （31.24-41.00 Mb） region on SSC7 and were found to be signiifcantly associated with one or more carcass traits and organ weights. High percentage of phenotypic variance explanation was observed for each trait ranging from 31.21 to 67.42%. Linkage analysis revealed one haplotype block of 495 kb, in which the most signiifcant SNP being MARC0033464 was contained, on SSC7 at complete linkage disequilibrium. Annotation of the pig reference genome suggested 6 genes （GRM4, HMGA1, NUDT3, RPS10, SPDEF and PACSIN1） in this candidate linkage disequilibrium （LD） interval. Functional analysis indicated that the HMGA1 gene presents the prime biological candidate for carcass traits and organ weights in pig, with potential application in breeding programs.

Imprinting Analysis of RTL1 and DIO3 Genes and Their Association with Carcass Traits in Pigs (Sus scrofa)

Imprinted genes play significant roles in the regulation of fetal growth, development, function of the placenta and postnatal behavior in mammals, but little is known in pigs. In order to investigate the imprinting status of porcine retro-transposon like 1 （RTL1） and type 3 iodothyronine deiodinase （DIO3） genes, DNA or RNA samples of the parents and F1 animals, generated with reciprocal crosses between Large White and Meishan breeds, were isolated, and analyzed by reverse transcription polymerase chain reaction restriction fragment length polymorphism （RT-PCR-RFLP）. The results demonstrated that the RTL1 gene was paternally expressed in 10 tissues, such as the skeletal muscle, heart, spleen, liver, kidney, lung, stomach, fat, small intestine and brain, and D103 gene exhibited paternal expression in the skeletal muscle, heart, spleen, lung, stomach, and brain, in 2-month-old pigs. The association of RTL1 and DI03 with carcass traits was further analyzed in the F2 population of Large White×Meishan pigs. The statistical results showed that the R TL1 A1101G polymorphism （EU781029） was significantly associated with lean meat percentage （LMP） and fat meat percentage （FMP） （P〈0.05）, while the D103 A744C polymorphism （AY533208） was not significantly associated with any carcass traits. These results indicate that the imprinting status of RTL1 and DIO3 is well kept across the mammalian species, and porcine RTL1 may have important roles in muscle growth and fat deposition.

Estimated Genetic Variance Explained by Single Nucleotide Polymorphisms of Different Minor Allele Frequencies for Carcass Traits in Japanese Black Cattle

Japanese Black cattle are a beef breed and well known to excel in carcass quality, but the details of genetic architectures for carcass traits in beef breeds including this breed are still poorly understood. The objective of this study was to estimate the degree of additive genetic variance explained by single nucleotide polymorphism (SNP) marker groups with different levels of minor allele frequency (MAF) for marbling score and carcass weight in Japanese Black cattle. Phenotypic data on 872 fattened steers with the genotype information about 40,000 autosomal SNPs were analyzed using two different statistical models: one considering only SNPs selected based on MAF (model 1) and the other also considering all remaining SNPs as the different term (model 2). All available SNPs were classified into 10 groups based on their MAFs. For both traits, the estimated proportions of additive genetic variance explained by SNPs selected based on their MAFs using model 1 were always higher than the estimated ones using model 2. For carcass weight, relatively high values of the proportion of the additive genetic variance were estimated when using SNPs with MAFs which were in the ranges of 0.20 to 0.25 and 0.25 to 0.30, which may be partly due to the three previously-reported quantitative trait loci candidate regions. The results could have provided some information on the genetic architecture for the carcass traits in Japanese Black cattle, although its validity may be limited, mainly due to the sample size and the use of simpler statistical models in this study.

Genetic dissection and genomic prediction for pork cuts and carcass morphology traits in pig

Background As pre-cut and pre-packaged chilled meat becomes increasingly popular,integrating the carcasscutting process into the pig industry chain has become a trend.Identifying quantitative trait loci(QTLs)of pork cuts would facilitate the selection of pigs with a higher overall value.However,previous studies solely focused on evaluating the phenotypic and genetic parameters of pork cuts,neglecting the investigation of QTLs influencing these traits.This study involved 17 pork cuts and 12 morphology traits from 2,012 pigs across four populations genotyped using CC1 PorcineSNP50 BeadChips.Our aim was to identify QTLs and evaluate the accuracy of genomic estimated breed values(GEBVs)for pork cuts.Results We identified 14 QTLs and 112 QTLs for 17 pork cuts by GWAS using haplotype and imputation genotypes,respectively.Specifically,we found that HMGA1,VRTN and BMP2 were associated with body length and weight.Subsequent analysis revealed that HMGA1 primarily affects the size of fore leg bones,VRTN primarily affects the number of vertebrates,and BMP2 primarily affects the length of vertebrae and the size of hind leg bones.The prediction accuracy was defined as the correlation between the adjusted phenotype and GEBVs in the validation population,divided by the square root of the trait’s heritability.The prediction accuracy of GEBVs for pork cuts varied from 0.342 to 0.693.Notably,ribs,boneless picnic shoulder,tenderloin,hind leg bones,and scapula bones exhibited prediction accuracies exceeding 0.600.Employing better models,increasing marker density through genotype imputation,and pre-selecting markers significantly improved the prediction accuracy of GEBVs.Conclusions We performed the first study to dissect the genetic mechanism of pork cuts and identified a large number of significant QTLs and potential candidate genes.These findings carry significant implications for the breeding of pork cuts through marker-assisted and genomic selection.Additionally,we have constructed the first reference populations for genomic selection of pork cuts in pigs.

Effects of ammonia exposure on carcass traits and fatty acid composition of broiler meat

We aimed to study the effects of ammonia on carcass traits, organ indices and fatty acid composition of broilers. Four hundred 21-d-old male Arbor Acres broilers with initial weight 563.52 ± 2.82 g were randomly allotted to 1 of 4 groups treated with ammonia at <3 mg/kg(control),25 ± 3. 50 ± 3, and75 ± 3 mg/kg concentrations. Each group consisted of 4 replicates of 25 birds. Broilers from 21 to 42 d were reared on the net floor in the respiration-metabolism chambers where similar environmental conditions were maintained. At 32 and 42 d of age, carcass traits and organ indices were determined for4 birds per pen. At 42 d of age, fatty acid composition in the breast and thigh muscle of broilers was measured. Results showed as follows: 1) At 32 d, the dressing percentage of broilers exposed to 25 and75 mg/kg ammonia were lower than those in the control group(P < 0.05); eviscerated yield percentage of broilers in the 25 mg/kg ammonia group was also lower(P < 0.05). At 42 d, the dressing percentage of broilers in the ammonia treatments and the thigh muscle percentage of broilers in the 50 and 75 mg/kg ammonia groups were lower(P < 0.05) than those in the control. Breast muscle percentage of broilers exposed to 25 and 50 mg/kg ammonia and eviscerated yield percentage exposed to 50 mg/kg ammonia were lower than those in the control(P < 0.05). 2) The kidney index of broilers(d 32) exposed to ammonia was greater(P < 0.05) than that of the control. At 42 d, hepatic index of broilers exposed to ammonia was increased(P < 0.05), and spleen index was decreased(P < 0.05). 3) At 42 d, stearic(C18:0)and saturated fatty acids(SFA) in the thigh muscle of broilers were higher, while the unsaturated fatty acid:saturated fatty acid(U:F) ratio and unsaturated fatty acid(UFA) were lower in the 50 mg/kg ammonia treatment than in the control group(P < 0.05). In conclusion, ammonia over 25 mg/kg could decline carcass traits and immune organ indices and increase the kidney and hepatic indices. Further,exposure to 50 mg/kg ammonia could also decrease breast and thigh muscle yield percentage while increasing SFA content and decreasing UFA content in the thigh muscle of broilers.

Effects of dietary nanocrystalline cellulose supplementation on growth performance,carcass traits,intestinal development and lipid metabolism of meat ducks

The influence of nanocrystalline cellulose(NCC) supplementation on growth performance, carcass traits,intestinal development, and lipid metabolism was assessed in 600 one-day-old male meat ducks(Cherry Valley ducks) from 1 to 35 d of age. Diets were supplemented with 0,200,500,800 and 1,500 mg/kg NCC during both the starter(1-14 d) and grower(15-35 d) phases. Each dietary treatment consisted of 8 replicate cages of 15 birds. Supplementation of NCC was associated with dose dependent increases in BW gain and feed intake(P < 0.01) during 1-14 d of age and in BW at 35 d of age. As NCC content increased,the percentage of breast meat weight(P < 0.05) and leg(with bone) weight(P < 0.05) linearly increased,while the percentage of abdominal fat weight(P < 0.01) linearly decre.ased in ducks at 35 d of age.Supplementation of NCC resulted in a dose-dependent increase in the weight(P < 0.05) and density(P < 0.01) of the cecum. The percentage of total hepatic lipid content(P < 0.01) at 14 d of age and serum triglyceride(TG) concentration(P = 0.052) at 35 d of age linearly decreased with increasing of dietary NCC addition. In conclusion, inclusion of 1,500 mg/kg NCC in feed resulted in the greatest improvements in duck performance, intestinal development and lipid deposition.

Infusion of short chain fatty acids in the ileum improves the carcass traits,meat quality and lipid metabolism of growing pigs

Short chain fatty acids(SCFA)are the main products of indigestible carbohydrates undergoing bacterial fermentation in the hindgut,which are related to some physiological functions.This study was designed to investigate the effects of SCFA infusion by ileum on the carcass traits,meat quality and lipid meta-bolism of growing pigs.In a 28-day study,24 growing barrows fitted with a T-cannula in distal ileum were divided into 4 treatments:1)Control,2)antibiotics(AB),3)AB+300 mL of SCFA1 solution(ABS1),4)AB+300 mL of SCFA2 solution(ABS2).The concentrations of acetate,propionate and butyrate in SCFA1 solution were respectively 61.84,18.62 and 12.55 mmol/L,and in SCFA2 were respectively 40.08,15.41 and 9.78 mmol/L The results showed that the SCFA infusion increased the average daily feed intake and average daily gain of pigs(P<0.05).Meanwhile,the SCFA treatments increased longissimus dorsi area(P<0.05)and carcass weight(P=0.058),decreased the drip loss of longissimus dorsi(P=0.059),and reduced serum concentrations of triglyceride,total cholesterol and urea nitrogen(P<0.05).Besides,the SCFA administration inhibited the mRNA expressions of fatty acid synthase(FAS)and acetyl-CoA carboxylase in longissimus dorsi(P<0.05),the mRNA expression of FAS in the liver(P<0.05),and the mRNA expression of hormone-sensitive lipase in abdominal fat(P<0.05).Short chain fatty acid infusion also enhanced the mRNA expression of carnitine palmitoyltransferase-1αin the liver(P<0.05),the mRNA expressions of peroxisome proliferator activated receptor gamma and lipoprotein lipase in abdominal fat(P<0.05),and the mRNA expressions of free fatty acid receptor 2,glucose-6-phosphatase and phosphoenolpyruvate carboxykinase 1 in the colon(P<0.05).These results suggested that SCFA administration in the ileum could improve the carcass traits and meat quality of growing pigs,which was possibly due to the fact that SCFA modulated lipid metabolism.

Comparisons of carcass traits,meat quality,and serum metabolome between Shaziling and Yorkshire pigs

This study aims to compare the meat quality of Shaziling and Yorkshire pigs and to find the potential indicator in serum for superior meat quality.Six Shaziling and Yorkshire pigs at 30,60,90,150,210,and 300 d of age were selected to examine carcass traits,meat quality,and serum metabolome.The results showed that the body weight,carcass length,and loin eye area of Shaziling pigs at 150,210,and 300 d of age were significantly lower than those of Yorkshire pigs(P<0.05).Shaziling pigs at 150 and 300 d of age had significantly lower backfat thickness than Yorkshire pigs(P<0.05).Compared with Yorkshire pigs,Shaziling pigs at all 6 ages had a lower lean percentage and a higher fat percentage(P<0.05).At 60,90,and 150 d of age,the post-mortem pH-decline,b*value(yellowness),and drip loss of Shaziling pigs were significantly lower than those of Yorkshire pigs(P<0.05).Moreover,at 150 d of age,Shaziling pigs had significantly higher a^(*)value(redness)and intramuscular fat(IMF)content than Yorkshire pigs(P<0.05).Correlation analysis between the top 40 metabolites and phenotypes indicated that L-carnitine had positive correlations with fat percentage,pH24h,and IMF content,but had negative correlations with lean percentage,L^(*)value(lightness),and b^(*)value(P<0.05).Serum L-carnitine content,fat percentage,pH24h,and IMF content all decreased first and then increased as the pigs grew,which verified the positive correlations between L-carnitine and these phenotypes.In conclusion,Shaziling pigs have a slower growth rate but a better meat quality than Yorkshire pigs.The meat quality of Shaziling pigs is the best from 150 to 210 d of age.This study suggests that a higher serum L-carnitine content is a promising indicator for better meat quality.

Chromium propionate improves performance and carcass traits in broilers

There is evidence to suggest that poultry may have a dietary requirement for metabolically available chromium(Cr)that exceeds the amount provided through wheat soybean meal diets.The objective of the present study was to investigate the effects of dietary supplemental organic Cr from Cr propionate at different dose levels(control=0μg/kg,T1=200μg/kg,T2=400μg/kg)on the growth performance,carcass traits,and meat quality of broilers.Weight gain and feed intake of each treatment were recorded at the start and after 14,28 and 35 d,and feed conversion ratios(FCR)were calculated accordingly.At35 d of age,birds were randomly selected and euthanized for carcass evaluation.Results of the first trial indicate that both Cr propionate treatments increased final body weight(P<0.05),feed efficiency(P<0.05)and body weight gain(P<0.0001).Furthermore,Cr propionate supplementation improved(P<0.0001)all carcass characteristics.Interestingly,with increased Cr dosage,carcass yield,dressing percentage and breast meat yield increased linearly(P<0.0001).The second study reveals that the feed intake in the control group was significantly higher compared to both Cr propionate supplemented groups(T1&T2).Furthermore,the Cr propionate supplemented T2 group displayed a significantly lower FCR than the control and T1 group(P=0.027).Finally,Cr propionate supplementation increased the dressing percentage compared to control birds(P<0.0001).In the third experiment,Cr propionate supplementation(T1&T2)increased final body weight and decreased FCR compared with the control treatment.These effects were highly significant(P<0.0001)throughout all feeding phases of the trial.Cr propionate supplementation also increased(P<0.0001)carcass yield,dressing percentage,breast meat yield,leg and thigh weights compared with the control treatment.In conclusion,growth performance,feed conversion,carcass yield,breast and leg meats of broiler birds can be significantly improved by dietary inclusion of Cr propionate.Cr propionate can be supplemented to broiler birds from 1 d old of age at a level that provides 200 or 400μg/kg organic Cr and can increase the efficiency of broiler production.

Detection of Quantitative Trait Loci Associated with Several Internal Organ Traits and Teat Number Trait in a Pig Population

Quantitative trait loci （QTL） were detected for 8 internal organ traits, 3 carcass length traits, and teat number trait in 214 pigs in a resource population that included 180 F2 individuals. A total of 39 microsatellite markers were examined on SSC4, SSC6, SSC7, SSC8, and SSC13. The genetic traits included heart weight （HW）, lung weight （LW）, liver and gallbladder weight （LGW）, spleen weight （SPW）, stomach weight （STW）, small intestine weight （S1W）, large intestine weight （LIW）, kidney weight （KW）, carcass length to the first cervical vertebra （CL1）, carcass length to the first thoracic vertebra （CL2）, rib numbers （RNS）, and teat numbers （TNS）. Results indicated that, 3 highly significant QTL （P≤0.01 at chromosome-wise level） for HW （at 30 cM on SSC6）, RNS （at 115 cM on SSC7）, TNS （at 110 cM on SSC7）, and 6 significant QTL （P≤0.05 at chromosome-wise level） for LW （at 119 cM on SSC13）, LGW （at 94 cM on SSC6）, SPW （at 106 cM on SSC8）, SIW （0 cM on SSC4）, LIW （170 cM on SSC 4）, and TNS （at 95 cM on SSC6） were detected. The phenotypic variances for which these QTL were accounted ranged from 0.04 % to 14.06 %. Most of these QTL had not been previously reported.