Differentially expressed genes(DEGs)between individuals with high(HC)and low(LC)total carotenoid content(TCC)were sampled from a selected line of Pinctada fucata martensii with black shell in the prismatic layer.The e...Differentially expressed genes(DEGs)between individuals with high(HC)and low(LC)total carotenoid content(TCC)were sampled from a selected line of Pinctada fucata martensii with black shell in the prismatic layer.The expression levels of candidate genes were verified by qRT-PCR.Targeted resequencing was used to detect SNPs in a candidate gene,PmSR-BI.The association of TCC with SNPs in PmSR-BI was determined.Results showed that a total of 1025 DEGs were identified between HC and LC.The expression levels of the candidate gene PmSR-BI in HC were higher than those in LC.Seven SNPs in the exon and eight SNPs in the 5′regulatory regions of PmSR-BI were found.Association analysis showed that one SNP in the exon and two SNPs in the 5′regulatory regions of PmSR-BI were significantly associated with the TCC(P<0.05).All SNPs of PmSR-BI were divided into four blocks.CC haplotype in Block 1 and AG haplotype in Block 3 were significantly higher than other haplotypes.These results help elucidate the mechanism underlying carotenoid metabolism and develop marker-assisted breeding design in the species.展开更多
A study was conducted to screen out the low-temperature tolerant Boro rice seedlings from November 2012 to January 2013 for facing the upcoming rice production challenge in Northwest Bangladesh. The experimental time ...A study was conducted to screen out the low-temperature tolerant Boro rice seedlings from November 2012 to January 2013 for facing the upcoming rice production challenge in Northwest Bangladesh. The experimental time was characterized by a prevailing low environmental temperature of below 15°C. Five rice cultivars (V1: BR-2;V2: BR-16;V3: Pariza;V4: Minicate;V5: BRRI dhan 50) were selected for the study. The leaf proline, chlorophyll content and total carotenoid content were investigated. The V2 (BR-16) seedling synthesized the higher leaf proline (1.228 g·g-1) at a low temperature than those of other tested cultivars. Again, the highest amount of chlorophyll-a (3.957 g·g-1), chlorophyll-b (2.118 g·g-1), chlorophyll-a/b ratio (3.6754 mg·g-1) and total chlorophyll (5.051 g·g-1) was measured in V2 (BR-16). The maximum total carotenoid (1.213 g·g-1) was also observed in V2. In this experiment, the V2 (BR-16) showed comparatively better potentiality to survive at low temperatures (below 15°C) than other varieties.展开更多
Their high bioactive compounds content and importance as dietary antioxidants has increased interest in Capsicum fruit. The fruit of seven Capsicum chinense Jacq. var. habanero genotypes grown in Yucatan, Mexico, were...Their high bioactive compounds content and importance as dietary antioxidants has increased interest in Capsicum fruit. The fruit of seven Capsicum chinense Jacq. var. habanero genotypes grown in Yucatan, Mexico, were analyzed to quantify their phenolic compounds, carotenoids and ascorbic acid contents, and to measure their free radical scavenging (ABTS assay) and antioxidant activities (β-carotene/linoleic acid assay). Phenolics (20.54 to 20.75 mg/100 g sample), carotenoids (1.00 to 1.26 mg/100 g sample) and ascorbic acid contents (187.24 to 281.73 mg/100 g sample) varied between genotypes. Trolox equivalent antioxidant capacity (TEAC) ranged from 1.55 to 3.23 mM/mg sample. During the 120-min decolorization trial, antioxidant capacity decreased over time in the studied genotypes. Values ranged from 36%to 57% β-carotene bleaching during the first 30 minutes. Fruit from all seven studied genotypes are good antioxidant sources and hold promise as natural ingredients in functional foods.展开更多
The current interest in the health beneifts of whole wheat grain has prompted breeders to further increase the concentration of antioxidants in wheat. The objective of this study was to investigate the variation in an...The current interest in the health beneifts of whole wheat grain has prompted breeders to further increase the concentration of antioxidants in wheat. The objective of this study was to investigate the variation in antioxidant content among Chinese wheat grains and the relationship between antioxidants and grain color and morphological characteristics. A wide variation was observed in the total phenolic, carotenoid and lfavonoid contents, as well as the antioxidant activity （AOA）, of Chinese wheat varieties. Black wheat had the highest mean total phenolic, carotenoid and lfavonoid contents and the highest AOA, followed by red and white wheats. The grain color parameters were signiifcantly negatively correlated with total phenolic, carotenoid and lfavonoid contents and AOA among all of the wheat varieties examined, and grain weight was also signiifcantly negatively correlated with these traits. The same correlation between grain weight and antioxidant traits was also observed within individual groups of wheat, which indicates that grain weight may be used as an index for selecting wheat varieties with high AOA. Landraces had signiifcantly higher lfavonoid content than commercial wheat varieties. The results of this study may be useful for breeding nutrient-rich wheat varieties.展开更多
基金The research was financially supported by Science and Technology Program of Guangdong Province(Grant No.2021B0202020003,and 2022A1515010030)National Natural Science Foundation of China(Grant No.32102817)+2 种基金Department of Education of Guangdong Province(Grant No.2019KQNCX043,2020ZDZX1045 and 2021KCXTD026)Special promotion of fishery science and technology of Ocean and Fisheries Bureau of Guangdong Province(Grant No.B201601-Z09)the earmarked fund for CARS-49.
文摘Differentially expressed genes(DEGs)between individuals with high(HC)and low(LC)total carotenoid content(TCC)were sampled from a selected line of Pinctada fucata martensii with black shell in the prismatic layer.The expression levels of candidate genes were verified by qRT-PCR.Targeted resequencing was used to detect SNPs in a candidate gene,PmSR-BI.The association of TCC with SNPs in PmSR-BI was determined.Results showed that a total of 1025 DEGs were identified between HC and LC.The expression levels of the candidate gene PmSR-BI in HC were higher than those in LC.Seven SNPs in the exon and eight SNPs in the 5′regulatory regions of PmSR-BI were found.Association analysis showed that one SNP in the exon and two SNPs in the 5′regulatory regions of PmSR-BI were significantly associated with the TCC(P<0.05).All SNPs of PmSR-BI were divided into four blocks.CC haplotype in Block 1 and AG haplotype in Block 3 were significantly higher than other haplotypes.These results help elucidate the mechanism underlying carotenoid metabolism and develop marker-assisted breeding design in the species.
文摘A study was conducted to screen out the low-temperature tolerant Boro rice seedlings from November 2012 to January 2013 for facing the upcoming rice production challenge in Northwest Bangladesh. The experimental time was characterized by a prevailing low environmental temperature of below 15°C. Five rice cultivars (V1: BR-2;V2: BR-16;V3: Pariza;V4: Minicate;V5: BRRI dhan 50) were selected for the study. The leaf proline, chlorophyll content and total carotenoid content were investigated. The V2 (BR-16) seedling synthesized the higher leaf proline (1.228 g·g-1) at a low temperature than those of other tested cultivars. Again, the highest amount of chlorophyll-a (3.957 g·g-1), chlorophyll-b (2.118 g·g-1), chlorophyll-a/b ratio (3.6754 mg·g-1) and total chlorophyll (5.051 g·g-1) was measured in V2 (BR-16). The maximum total carotenoid (1.213 g·g-1) was also observed in V2. In this experiment, the V2 (BR-16) showed comparatively better potentiality to survive at low temperatures (below 15°C) than other varieties.
基金This research was supported by the Fundación PRO-DUCE AC-Yucatán,México(Project“Determinación de las propiedades funcionales del chile habanero”).
文摘Their high bioactive compounds content and importance as dietary antioxidants has increased interest in Capsicum fruit. The fruit of seven Capsicum chinense Jacq. var. habanero genotypes grown in Yucatan, Mexico, were analyzed to quantify their phenolic compounds, carotenoids and ascorbic acid contents, and to measure their free radical scavenging (ABTS assay) and antioxidant activities (β-carotene/linoleic acid assay). Phenolics (20.54 to 20.75 mg/100 g sample), carotenoids (1.00 to 1.26 mg/100 g sample) and ascorbic acid contents (187.24 to 281.73 mg/100 g sample) varied between genotypes. Trolox equivalent antioxidant capacity (TEAC) ranged from 1.55 to 3.23 mM/mg sample. During the 120-min decolorization trial, antioxidant capacity decreased over time in the studied genotypes. Values ranged from 36%to 57% β-carotene bleaching during the first 30 minutes. Fruit from all seven studied genotypes are good antioxidant sources and hold promise as natural ingredients in functional foods.
基金funded by the Special Funds for AgroScientifc Research in the Public Interest,China (201203031)the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD16B07-2)the National Agriculture Technology Research System of China (CARS-03)
文摘The current interest in the health beneifts of whole wheat grain has prompted breeders to further increase the concentration of antioxidants in wheat. The objective of this study was to investigate the variation in antioxidant content among Chinese wheat grains and the relationship between antioxidants and grain color and morphological characteristics. A wide variation was observed in the total phenolic, carotenoid and lfavonoid contents, as well as the antioxidant activity （AOA）, of Chinese wheat varieties. Black wheat had the highest mean total phenolic, carotenoid and lfavonoid contents and the highest AOA, followed by red and white wheats. The grain color parameters were signiifcantly negatively correlated with total phenolic, carotenoid and lfavonoid contents and AOA among all of the wheat varieties examined, and grain weight was also signiifcantly negatively correlated with these traits. The same correlation between grain weight and antioxidant traits was also observed within individual groups of wheat, which indicates that grain weight may be used as an index for selecting wheat varieties with high AOA. Landraces had signiifcantly higher lfavonoid content than commercial wheat varieties. The results of this study may be useful for breeding nutrient-rich wheat varieties.
