The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accur...The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors.In this study,the expression profiles of ten candidate genes,ACT1,ACT2,GAPDH,eIF1,eIF2,α-TUB,β-TUB,TBP,RNA Pol II and RP II,were calculated for a suitable reference gene selection in Paeonia ostii T.Hong et J.X.Zhang leaves under various drought stress conditions.Data were processed by the four regularly used evaluation software.A comprehensive analysis revealed that RNA Pol II was the most stable gene and eIF2 was the least stable one.In addition,the geNorm program provided the optimal choice of two reference gene combination,RNA Pol II andβ-TUB,for qRT-PCR normalization in P.ostii subjected to different drought stress levels.Our research provided convenience for gene expression analysis in P.ostii under drought stress and promoted research of effective methods to alleviate P.ostii drought stress in the future.展开更多
This paper discusses ecological and monitoring studies covering vegetation, stream fauna, birds, mammals and other vertebrates at the West New Territories (WENT)Landfill, Hong Kong. Recommendations for the protection ...This paper discusses ecological and monitoring studies covering vegetation, stream fauna, birds, mammals and other vertebrates at the West New Territories (WENT)Landfill, Hong Kong. Recommendations for the protection of vegetation on and around the site, including control of the erosion and run off from the construction site were proposed and implemented, resulting in effective protection of the local vegetation cover. Stream macrobenthos were low in species diversity and abundance and did not show a changing trend during the construction and the operation of the land fill. Birds attracted to the active dumping site were dominated by Crested Mynah Acridotheres cristatellus and the Tree Sparrow Passer montanus. After an increase in the population during the warmer months, the number of birds seen on the land fill site decreased to 10 in December 1994. Mammal monitoring by live trapping revealed that the rat population did not increase after the land fill operation started. This project was the first comprehensive’ ecological study and monitoring of an urban land fill in Hong Kong and it demonstrated that careful environmental planning and implementation of mitigation measures can successfully limit environmental impacts of landfills to acceptable levels.展开更多
基金This work was supported by the National Key R&D Program of China(2018YFD1000405)Jiangsu Modern Agricultural Industrial Technology System(JATS[2020]436)。
文摘The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors.In this study,the expression profiles of ten candidate genes,ACT1,ACT2,GAPDH,eIF1,eIF2,α-TUB,β-TUB,TBP,RNA Pol II and RP II,were calculated for a suitable reference gene selection in Paeonia ostii T.Hong et J.X.Zhang leaves under various drought stress conditions.Data were processed by the four regularly used evaluation software.A comprehensive analysis revealed that RNA Pol II was the most stable gene and eIF2 was the least stable one.In addition,the geNorm program provided the optimal choice of two reference gene combination,RNA Pol II andβ-TUB,for qRT-PCR normalization in P.ostii subjected to different drought stress levels.Our research provided convenience for gene expression analysis in P.ostii under drought stress and promoted research of effective methods to alleviate P.ostii drought stress in the future.
文摘This paper discusses ecological and monitoring studies covering vegetation, stream fauna, birds, mammals and other vertebrates at the West New Territories (WENT)Landfill, Hong Kong. Recommendations for the protection of vegetation on and around the site, including control of the erosion and run off from the construction site were proposed and implemented, resulting in effective protection of the local vegetation cover. Stream macrobenthos were low in species diversity and abundance and did not show a changing trend during the construction and the operation of the land fill. Birds attracted to the active dumping site were dominated by Crested Mynah Acridotheres cristatellus and the Tree Sparrow Passer montanus. After an increase in the population during the warmer months, the number of birds seen on the land fill site decreased to 10 in December 1994. Mammal monitoring by live trapping revealed that the rat population did not increase after the land fill operation started. This project was the first comprehensive’ ecological study and monitoring of an urban land fill in Hong Kong and it demonstrated that careful environmental planning and implementation of mitigation measures can successfully limit environmental impacts of landfills to acceptable levels.
