OBJECTIVE To investigate the relationship between the level of caspase-3 and 8 expression and intracellular Ca^2＋ levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into t...OBJECTIVE To investigate the relationship between the level of caspase-3 and 8 expression and intracellular Ca^2＋ levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into three IFNa-treated groups and one control group as follows： an intratumoral injection, subcutaneous injection, preventive injection, and a control without injection. The cellular DNA content, changes in the cell cycle and the relationship between the cell Ca^2＋ concentrations and the expression of cespase-3 and 8 were examined. RESULTS After injection of IFN-α-2b by different routes, the morphologic transformation of the breast cancer cells in each group was observed. There was a typical apoptotic response in the intratumoral-Jnjection group. The expression of cespase-3 and 8 was diverse among the experimental groups, and correlated with the cellular Ca^2＋ concentration. Caspase-3 and 8 expression and the cellular Ca^2＋ level were higher following intratumoral injection compared to the other treatments （P〈0.01）. Among the experimental groups, the cell cycle displayed definitive changes. CONCLUSION a） Both caspase-3 and 8 and the intracellular Ca^2＋ are elevated in the process of cell apoptosis in BCML-TA299 breast cancer tissues. These changes may play important roles in the occurrence and development of breast cancer; b） Variation in the route of IFN-α-2b administration can produce different responses in the expression of caspase-3 and 8 and the concentration of Ca^2＋ in apoptotic tumor cells.展开更多
背景:3D打印技术可根据患者实际病情和治疗需求设计构建模型、手术导板和个性化植入体或固定物,在创伤性骨折修复中展示了巨大的应用前景。目的:综述3D打印技术在创伤性骨折中的应用。方法:检索Web of science、PubMed和中国知网数据库2...背景:3D打印技术可根据患者实际病情和治疗需求设计构建模型、手术导板和个性化植入体或固定物,在创伤性骨折修复中展示了巨大的应用前景。目的:综述3D打印技术在创伤性骨折中的应用。方法:检索Web of science、PubMed和中国知网数据库2020-2024年发表的创伤骨科领域3D打印技术应用的相关文献,英文检索词为“traumatic fracture,3D printing technology,digital model,surgical guide”,中文检索词为“创伤性骨折,3D打印技术,数字模型,手术导板”,经筛选和分析,最终纳入60篇文献进行分析。结果与结论:①创伤性骨折是各种致伤因素导致的骨骼连续性中断和完整性破坏的骨折现象,以可靠方案提高复位愈合效果,已成为骨外科相关研究领域亟需解决的热点问题;②3D打印技术是以数字模型数据为基础的,运用粉末状金属或聚合物等可黏合成型材料以立体光刻、沉积建模和光聚合物喷射等形式制造满足需求三维实体的技术,在数字骨科生物医学领域应用广泛;③3D打印技术在疾病诊断、术前规划、重建骨折三维模型、定制骨科植入体、定制固定支具及假肢、手术导板制作和骨缺损修复等方面发挥了显著的优势,可根据患者实际病情和治疗需求设计构建模型、手术导板和个性化植入体或固定物,为创伤性骨折的治疗提供了新的思路。展开更多
文摘OBJECTIVE To investigate the relationship between the level of caspase-3 and 8 expression and intracellular Ca^2＋ levels in BCML-TA299 breast cancer cells in the process of apoptosis. METHODS Mice were divided into three IFNa-treated groups and one control group as follows： an intratumoral injection, subcutaneous injection, preventive injection, and a control without injection. The cellular DNA content, changes in the cell cycle and the relationship between the cell Ca^2＋ concentrations and the expression of cespase-3 and 8 were examined. RESULTS After injection of IFN-α-2b by different routes, the morphologic transformation of the breast cancer cells in each group was observed. There was a typical apoptotic response in the intratumoral-Jnjection group. The expression of cespase-3 and 8 was diverse among the experimental groups, and correlated with the cellular Ca^2＋ concentration. Caspase-3 and 8 expression and the cellular Ca^2＋ level were higher following intratumoral injection compared to the other treatments （P〈0.01）. Among the experimental groups, the cell cycle displayed definitive changes. CONCLUSION a） Both caspase-3 and 8 and the intracellular Ca^2＋ are elevated in the process of cell apoptosis in BCML-TA299 breast cancer tissues. These changes may play important roles in the occurrence and development of breast cancer; b） Variation in the route of IFN-α-2b administration can produce different responses in the expression of caspase-3 and 8 and the concentration of Ca^2＋ in apoptotic tumor cells.
