A simplified model of the thrust force is proposed based on a caudal fin oscillation of an underwater bionic robot. The caudal fin oscillation is generalized by cen- tral pattern generators (CPGs). In this model, th...A simplified model of the thrust force is proposed based on a caudal fin oscillation of an underwater bionic robot. The caudal fin oscillation is generalized by cen- tral pattern generators (CPGs). In this model, the drag coefficient and lift coefficient are the two critical parameters which are obtained by the digital particle image velocimetry (DPIV) and the force transducer experiment. Numerical simulation and physical experi- ments have been performed to verify this dynamic model.展开更多
A cell line,termed ZFIN,was established from the caudal fin of zebrafish and was shown to be susceptible to spring viremia of carp virus(SVCV).The ZFIN cells are epithelial like cells and have a moderate plasmid trans...A cell line,termed ZFIN,was established from the caudal fin of zebrafish and was shown to be susceptible to spring viremia of carp virus(SVCV).The ZFIN cells are epithelial like cells and have a moderate plasmid transfection efficiency of 13.9%.Using an RNA-seq approach,differentially expressed genes(DEGs)regulated by SVCV were identified.Infection of SVCV gave rise to 3931 DEGs and up-regulated DEGs were mostly enriched into the biological regulation and cellular processes,among which pathways for the type I interferon signaling and the response to exogenous dsRNA were the top two GO terms.Several KEGG signaling pathways including TLR signaling pathway,RLR receptor signaling pathway,cytosolic DNA-sensing pathway,NLR signaling pathway,cytokine-cytokine receptor interaction and ferroptosis were significantly enriched.Antiviral genes including ifnφ1,isg15 and mx were significantly up-regulated.In addition,key DEGs involved in autophagy were identified.The results indicate that the ZFIN cell line provides a useful in vitro tool for study on the gene functions and cellular responses to viral infection in fish.展开更多
This article presents a comprehensive study of the effects of the caudal fin shape on the propulsion performance of a candal fin in harmonic heaving and pitching. A numerical simulation based on an unsteady panel meth...This article presents a comprehensive study of the effects of the caudal fin shape on the propulsion performance of a candal fin in harmonic heaving and pitching. A numerical simulation based on an unsteady panel method was carried out to analyze the hydrodynamic performance of flapping caudal fins of three shapes (the whale caudal fin with the largest projected area, the dolphin caudal fin with the median projected area, and the tuna caudal fin with the smallest projected area). Then, a series of hydrodynamic experiments for three caudal fin shapes were performed. Both computational and experimental results indicate that the tuna caudal fin produces the highest efficiency. However the mean thrust coefficient of the tuna caudal fin is the smallest. It is found that although the mean thrust coefficient for the tuna caudal fin is not large, the input power of the tuna caudal fin is also quite small. So the tuna caudal fin achieves a high efficiency.展开更多
A comprehensive numerical simulation of the hydrodynamic performance of a caudal fin with unsymmetric flapping motion is carried out. The unsymmetrical motion is induced by adding a pitch bias or a heave bias. A numer...A comprehensive numerical simulation of the hydrodynamic performance of a caudal fin with unsymmetric flapping motion is carried out. The unsymmetrical motion is induced by adding a pitch bias or a heave bias. A numerical simulation program based on the unsteady panel method is developed to simulate the hydrodynamics of an unsymmetrical flapping caudal fin. A CFD code based on Navier-Stokes equations is used to analyze the flow field. Computational results of both the panel method and the CFD method indicate that the hydrodynamics are greatly affected by the pitch bias and the heave bias. The mean lateral force coefficient is not zero as in contrast with the symmetrical flapping motion. By increasing the pitch bias angle, the mean thrust force coefficient is reduced rapidly. By adding a heave bias, the hydrodynamic coefficients are separated as two parts: in one part, the amplitude is the heave amplitude plus the bias and in the other part, it is the heave amplitude minus the bias. Analysis of the flow field shows that the vortex distribution is not symmetrical, which generates the non-zero mean lateral force coefficient.展开更多
A test for the wake vortex of a flexible oscillating caudal fin is carried out with Digital Particle Image Velocimetry (DPIV), and the variation of vortex distance and the vorticity in the range of oscillating frequ...A test for the wake vortex of a flexible oscillating caudal fin is carried out with Digital Particle Image Velocimetry (DPIV), and the variation of vortex distance and the vorticity in the range of oscillating frequency from 0.704 Hz to 1.17 Hz are analyzed. It is found that with the increase of the oscillating frequency, the vortex distance decreases and the peak of the vorticity increases, When the Strouhal number is smaller than 0.49, a larger thrust component is obtained. The distribution of the velocity circulation and the vortex distance in the different spanwise section of the caudal fin are given, and then the dimension of the vortex ring is determined. The radius of the vortex ring is 79.3 mm and the average velocity circulation is 28152.9 mm2/s at the oscillating frequency of 0.835 Hz. The model of 3-D vortex ring chain of flexible oscillating caudal fin is constructed based on the information of wake vortex field. Finally, an effective analysis method is provided for establishing the relationship of oscillating parameters for the caudal fin and the wake structure and the intrinsic mechanism of efficient fish swimming is investigated.展开更多
Shape optimization of the caudal fin of the three-dimensional self-propelled swimming fish,to increase the swimming efficiency and the swimming speed and control the motion direction more easily,is investigated by com...Shape optimization of the caudal fin of the three-dimensional self-propelled swimming fish,to increase the swimming efficiency and the swimming speed and control the motion direction more easily,is investigated by combining optimization algorithms,unsteady computational fluid dynamics and dynamic control in this study.The 3D computational fluid dynamics package contains the immersed boundary method,volume of fluid method,the adaptive multi-grid finite volume method and the control strategy of fish swimming.Through shape optimizations of various swimming speeds,the results show that the optimal caudal fins of different swimming modes are not exactly the same shape.However,the optimal fish of high swimming speed,whose caudal fin shape is similar to the crescent,also have higher efficiency and better maneuverability than the other optimal bionic fish at low and moderate swimming speeds.Finally,the mechanisms of vorticity creation of different optimal bionic fish are studied by using boundary vorticity-flux theory,and three-dimensional wake structures of self-propelled swimming of these fish are comparatively analyzed.The study of vortex dynamics reveals the nature of efficient swimming of the 3D bionic fish with the lunate caudal fin.展开更多
This paper examines the beneficial effects of the spanwise flexibility of the caudal fin for the improvement of the swimming performance for small fishlike robots. A virtual swimmer is adopted for controlled numerical...This paper examines the beneficial effects of the spanwise flexibility of the caudal fin for the improvement of the swimming performance for small fishlike robots. A virtual swimmer is adopted for controlled numerical experiments by varying the spanwise flexible trajectories and the spanwise flexible size of the caudal fin while keeping the body kinematics fixed. 3-D Navier-Stokes equations are used to compute the viscous flow over the robot. Elliptical, parabolic and hyperbola trajectories are chosen to describe the spanwise flexible profile of the caudal fin. According to the sign(positive or negative) of the phase difference of the swinging motion, the spanwise flexibility can be divided into the fin surface of "bow" and the fin surface of "scoop". It is observed that for both the fin surface of "bow" and the fin surface of "scoop", the spanwise elliptical trajectory has the optimal swimming velocity, thrust, lateral force, and efficiency. With comparisons, using the flexible caudal fin with the fin surface of "bow", the lateral force and the power consumption can be reduced effectively and the swimming stability can be increased while reducing little the swimming velocity and thrust. Meanwhile, using the flexible caudal fin with the fin surface of "scoop" can greatly improve the swimming velocity, thrust, and efficiency while increasing part of the lateral force and the power consumption. Three-dimensional flow structures clearly indicate the evolution process around the swimming robot. It is suggested that the fish, the dolphin, and other aquatic animals may benefit their hydrodynamic performance by the spanwise flexibility of the caudal fin.展开更多
To investigate the effects of the caudal fin deformation on the hydrodynamic performance of the self-propelled thunniform swimming,we perform fluid-body interaction simulations for a tuna-like swimmer with thunniform ...To investigate the effects of the caudal fin deformation on the hydrodynamic performance of the self-propelled thunniform swimming,we perform fluid-body interaction simulations for a tuna-like swimmer with thunniform kinematics.The 3-D vortices are visualized to reveal the role of the leading-edge vortex(LEV)in the thrust generation.By comparing the swimming velocity of the swimmer with different caudal fin flexure amplitudes fa,it is shown that the acceleration in the starting stage of the swimmer increases with the increase of fa,but its cruising velocity decreases.The results indicate that the caudal fin deformation is beneficial to the fast start but not to the fast cruising of the swimmer.During the entire swimming process,the undulation amplitudes of the lateral velocity and the yawing angular velocity decrease as fa increases.It is found that the formation of an attached LEV on the caudal fin is responsible for generating the low-pressure region on the surface of the caudal fin,which contributes to the thrust.Furthermore,the caudal fin deformation can delay the LEV shedding from the caudal fin,extending the duration of the low pressure on the caudal fin,which will cause the caudal fin to generate a drag-type force over a time period in one swimming cycle and reduce the cruising speed of the swimmer.展开更多
The hydrodynamic performance of the virtual underwater vehicle under self-yaw is investigated numerically in this paper,we aim to explore the fluid laws behind this plane motion achieved by the bionic flexibility,espe...The hydrodynamic performance of the virtual underwater vehicle under self-yaw is investigated numerically in this paper,we aim to explore the fluid laws behind this plane motion achieved by the bionic flexibility,especially the spanwise flexibility of the caudal fin.The kinematics of the chordwise flexible body and the spanwise flexible caudal fin are explored through dynamic mesh technology and user-defined functions(UDF).The 3-D Navier-Stokes equations are applied to simulate the viscid fluid surrounding the bionic dolphin.The study focuses on quantitative problems about the fluid dynamics behind the specific motion law,including speed of movement,energy loss and working efficiency.The current results show that the self-yaw can be composed of two motions,autonomous propulsion and active steering.In addition,the degree of the flexible caudal fin can produce different yaw effects.The chordwise phase differenceФis dominant in the propulsion function,while the spanwise phase differenceδhas a more noticeable effect on the steering function.The pressure distribution on the surface of the dolphin and the wake vortex generated in the flow field reasonably reveal the evolution of self-yaw.It properly turns out that the dolphin can combine the spanwise flexible caudal fin and the chordwise flexible body to achieve self-yaw motion.展开更多
Based on the boundary vorticity-flux theory,topology optimization of the caudal fin of the three-dimensional self-propelled swimming fish is investigated by combining unsteady computational fluid dynamics with moving ...Based on the boundary vorticity-flux theory,topology optimization of the caudal fin of the three-dimensional self-propelled swimming fish is investigated by combining unsteady computational fluid dynamics with moving boundary and topology optimization algorithms in this study.The objective functional of topology optimization is the function of swimming efficiency,swimming speed and motion direction control.The optimal caudal fin,whose topology is different from that of the natural fish caudal fin,make the 3D bionic fish achieve higher swimming efficiency,faster swimming speed and better maneuverability.The boundary vorticity-flux on the body surface of the 3D fish before and after optimization reveals the mechanism of high performance swimming of the topology optimization bionic fish.The comparative analysis between the swimming performance of the 3D topology optimization bionic fish and the 3D lunate tail bionic fish is also carried out,and the wake structures of two types of bionic fish show the physical nature that the swimming performance of the 3D topology optimization bionic fish is significantly better than the 3D lunate tail bionic fish.展开更多
In the traditional flow-resistance-differential (FRD) type valve-less piezoelectric pump, the generated outflow and pressure are discontinuous because of the inherent periodicity and fluctuation of the pump. To overco...In the traditional flow-resistance-differential (FRD) type valve-less piezoelectric pump, the generated outflow and pressure are discontinuous because of the inherent periodicity and fluctuation of the pump. To overcome these drawbacks, utilizing the bending vibration of piezoelectric bimorph to drive fluid was conducted. However, our investigation on the current status of this piezoelectric bimorph pump shows that larger driving force and vibration amplitude are required for fluid pumping; the pumping can be realized through the centrifugal force; and the mechanism of fluid pumping is no longer further studied. Based on these cases, the paper designed a piezoelectric-stack pump with variable-cross-section oscillating (VCSO) vibrator by imitating the swing of the caudal-fin of tuna, and the pump is neither the rotating type nor the volumetric type according to the taxonomy. The interaction between the oscillating vibrator and the fluid parcel is firstly analyzed from the viewpoint of momentum conservation, and the analytical expression of pump flow rate is obtained. Then the modal and harmonic response analyses on the vibrator immerged in water are carried out. From the analyses the first two orders resonance frequencies are 832 Hz and 1 939 Hz, respectively, and the peak value of the tip amplitude is 0.6 mm. Laser Doppler vibrometer is used to measure both the frequency and vibration amplitude, and the determined first two orders resonance frequencies are 617 Hz and 1 356 Hz, respectively. The measured tip amplitude reaches to the peak value of 0.3 mm. At last, experimental measurement for the flow rates with different driving frequencies is conducted. The results show that the flow rate can reach 560 mL/min at 1 370 Hz when the pump runs under the backpressure of 30 mm water column. And the flow rate is as much as 560% of that of experiment results carried out by researchers from Brazil. The proposed pump innovates in both theory and taxonomy; in addition, the pump overcomes the drawbacks such as large flow fluctuation and low flow rate in the traditional FRD type pumps, which will help to broaden the application of the valve-less piezoelectric pump.展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.61503008 and 51575005)the China Postdoctoral Science Foundation(No.2015M570013)
文摘A simplified model of the thrust force is proposed based on a caudal fin oscillation of an underwater bionic robot. The caudal fin oscillation is generalized by cen- tral pattern generators (CPGs). In this model, the drag coefficient and lift coefficient are the two critical parameters which are obtained by the digital particle image velocimetry (DPIV) and the force transducer experiment. Numerical simulation and physical experi- ments have been performed to verify this dynamic model.
基金funded by the National Natural Science Foundation of China(Grant No.32030112 and U21A20268).
文摘A cell line,termed ZFIN,was established from the caudal fin of zebrafish and was shown to be susceptible to spring viremia of carp virus(SVCV).The ZFIN cells are epithelial like cells and have a moderate plasmid transfection efficiency of 13.9%.Using an RNA-seq approach,differentially expressed genes(DEGs)regulated by SVCV were identified.Infection of SVCV gave rise to 3931 DEGs and up-regulated DEGs were mostly enriched into the biological regulation and cellular processes,among which pathways for the type I interferon signaling and the response to exogenous dsRNA were the top two GO terms.Several KEGG signaling pathways including TLR signaling pathway,RLR receptor signaling pathway,cytosolic DNA-sensing pathway,NLR signaling pathway,cytokine-cytokine receptor interaction and ferroptosis were significantly enriched.Antiviral genes including ifnφ1,isg15 and mx were significantly up-regulated.In addition,key DEGs involved in autophagy were identified.The results indicate that the ZFIN cell line provides a useful in vitro tool for study on the gene functions and cellular responses to viral infection in fish.
基金supported by the National Nature Science Foundation of China (Grant No.50879014)the Doctoral Program of Higher Education of China (Grant No.200802170010)
文摘This article presents a comprehensive study of the effects of the caudal fin shape on the propulsion performance of a candal fin in harmonic heaving and pitching. A numerical simulation based on an unsteady panel method was carried out to analyze the hydrodynamic performance of flapping caudal fins of three shapes (the whale caudal fin with the largest projected area, the dolphin caudal fin with the median projected area, and the tuna caudal fin with the smallest projected area). Then, a series of hydrodynamic experiments for three caudal fin shapes were performed. Both computational and experimental results indicate that the tuna caudal fin produces the highest efficiency. However the mean thrust coefficient of the tuna caudal fin is the smallest. It is found that although the mean thrust coefficient for the tuna caudal fin is not large, the input power of the tuna caudal fin is also quite small. So the tuna caudal fin achieves a high efficiency.
基金supported by the National Nature Science Foundation of China(Grant No.50879014)the Doctoral Program of Higher Education of China(Grant No.200802170010)
文摘A comprehensive numerical simulation of the hydrodynamic performance of a caudal fin with unsymmetric flapping motion is carried out. The unsymmetrical motion is induced by adding a pitch bias or a heave bias. A numerical simulation program based on the unsteady panel method is developed to simulate the hydrodynamics of an unsymmetrical flapping caudal fin. A CFD code based on Navier-Stokes equations is used to analyze the flow field. Computational results of both the panel method and the CFD method indicate that the hydrodynamics are greatly affected by the pitch bias and the heave bias. The mean lateral force coefficient is not zero as in contrast with the symmetrical flapping motion. By increasing the pitch bias angle, the mean thrust force coefficient is reduced rapidly. By adding a heave bias, the hydrodynamic coefficients are separated as two parts: in one part, the amplitude is the heave amplitude plus the bias and in the other part, it is the heave amplitude minus the bias. Analysis of the flow field shows that the vortex distribution is not symmetrical, which generates the non-zero mean lateral force coefficient.
基金supported by the National Natural Science Foundation of China(Grant No.50879031)the Key laboratory of Jiangsu Province(Grant No.CT0701)
文摘A test for the wake vortex of a flexible oscillating caudal fin is carried out with Digital Particle Image Velocimetry (DPIV), and the variation of vortex distance and the vorticity in the range of oscillating frequency from 0.704 Hz to 1.17 Hz are analyzed. It is found that with the increase of the oscillating frequency, the vortex distance decreases and the peak of the vorticity increases, When the Strouhal number is smaller than 0.49, a larger thrust component is obtained. The distribution of the velocity circulation and the vortex distance in the different spanwise section of the caudal fin are given, and then the dimension of the vortex ring is determined. The radius of the vortex ring is 79.3 mm and the average velocity circulation is 28152.9 mm2/s at the oscillating frequency of 0.835 Hz. The model of 3-D vortex ring chain of flexible oscillating caudal fin is constructed based on the information of wake vortex field. Finally, an effective analysis method is provided for establishing the relationship of oscillating parameters for the caudal fin and the wake structure and the intrinsic mechanism of efficient fish swimming is investigated.
基金supported by the National Natural Science Foundation of China (Grant No. 10672183)
文摘Shape optimization of the caudal fin of the three-dimensional self-propelled swimming fish,to increase the swimming efficiency and the swimming speed and control the motion direction more easily,is investigated by combining optimization algorithms,unsteady computational fluid dynamics and dynamic control in this study.The 3D computational fluid dynamics package contains the immersed boundary method,volume of fluid method,the adaptive multi-grid finite volume method and the control strategy of fish swimming.Through shape optimizations of various swimming speeds,the results show that the optimal caudal fins of different swimming modes are not exactly the same shape.However,the optimal fish of high swimming speed,whose caudal fin shape is similar to the crescent,also have higher efficiency and better maneuverability than the other optimal bionic fish at low and moderate swimming speeds.Finally,the mechanisms of vorticity creation of different optimal bionic fish are studied by using boundary vorticity-flux theory,and three-dimensional wake structures of self-propelled swimming of these fish are comparatively analyzed.The study of vortex dynamics reveals the nature of efficient swimming of the 3D bionic fish with the lunate caudal fin.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.51875101,51375085)
文摘This paper examines the beneficial effects of the spanwise flexibility of the caudal fin for the improvement of the swimming performance for small fishlike robots. A virtual swimmer is adopted for controlled numerical experiments by varying the spanwise flexible trajectories and the spanwise flexible size of the caudal fin while keeping the body kinematics fixed. 3-D Navier-Stokes equations are used to compute the viscous flow over the robot. Elliptical, parabolic and hyperbola trajectories are chosen to describe the spanwise flexible profile of the caudal fin. According to the sign(positive or negative) of the phase difference of the swinging motion, the spanwise flexibility can be divided into the fin surface of "bow" and the fin surface of "scoop". It is observed that for both the fin surface of "bow" and the fin surface of "scoop", the spanwise elliptical trajectory has the optimal swimming velocity, thrust, lateral force, and efficiency. With comparisons, using the flexible caudal fin with the fin surface of "bow", the lateral force and the power consumption can be reduced effectively and the swimming stability can be increased while reducing little the swimming velocity and thrust. Meanwhile, using the flexible caudal fin with the fin surface of "scoop" can greatly improve the swimming velocity, thrust, and efficiency while increasing part of the lateral force and the power consumption. Three-dimensional flow structures clearly indicate the evolution process around the swimming robot. It is suggested that the fish, the dolphin, and other aquatic animals may benefit their hydrodynamic performance by the spanwise flexibility of the caudal fin.
基金Project supported by the National Natural Science Foundation of China(Grant No.51479039).
文摘To investigate the effects of the caudal fin deformation on the hydrodynamic performance of the self-propelled thunniform swimming,we perform fluid-body interaction simulations for a tuna-like swimmer with thunniform kinematics.The 3-D vortices are visualized to reveal the role of the leading-edge vortex(LEV)in the thrust generation.By comparing the swimming velocity of the swimmer with different caudal fin flexure amplitudes fa,it is shown that the acceleration in the starting stage of the swimmer increases with the increase of fa,but its cruising velocity decreases.The results indicate that the caudal fin deformation is beneficial to the fast start but not to the fast cruising of the swimmer.During the entire swimming process,the undulation amplitudes of the lateral velocity and the yawing angular velocity decrease as fa increases.It is found that the formation of an attached LEV on the caudal fin is responsible for generating the low-pressure region on the surface of the caudal fin,which contributes to the thrust.Furthermore,the caudal fin deformation can delay the LEV shedding from the caudal fin,extending the duration of the low pressure on the caudal fin,which will cause the caudal fin to generate a drag-type force over a time period in one swimming cycle and reduce the cruising speed of the swimmer.
基金This work was supported by National Natural Science Founda-tion of China[grant number 51875101]State Key Laboratory of Robotics and System(HIT)[grant number SKLRS-2018-KF-11].
文摘The hydrodynamic performance of the virtual underwater vehicle under self-yaw is investigated numerically in this paper,we aim to explore the fluid laws behind this plane motion achieved by the bionic flexibility,especially the spanwise flexibility of the caudal fin.The kinematics of the chordwise flexible body and the spanwise flexible caudal fin are explored through dynamic mesh technology and user-defined functions(UDF).The 3-D Navier-Stokes equations are applied to simulate the viscid fluid surrounding the bionic dolphin.The study focuses on quantitative problems about the fluid dynamics behind the specific motion law,including speed of movement,energy loss and working efficiency.The current results show that the self-yaw can be composed of two motions,autonomous propulsion and active steering.In addition,the degree of the flexible caudal fin can produce different yaw effects.The chordwise phase differenceФis dominant in the propulsion function,while the spanwise phase differenceδhas a more noticeable effect on the steering function.The pressure distribution on the surface of the dolphin and the wake vortex generated in the flow field reasonably reveal the evolution of self-yaw.It properly turns out that the dolphin can combine the spanwise flexible caudal fin and the chordwise flexible body to achieve self-yaw motion.
基金support of National Natural Science Foundation of China(Grant No.11302071)and National Postdoctoral Foundation of China(Grant No.2013M541597).Our deep appreciation goes to Professor Wei SHYY of Hong Kong University of Science and Technology,for detailed discussion and kindly help.
文摘Based on the boundary vorticity-flux theory,topology optimization of the caudal fin of the three-dimensional self-propelled swimming fish is investigated by combining unsteady computational fluid dynamics with moving boundary and topology optimization algorithms in this study.The objective functional of topology optimization is the function of swimming efficiency,swimming speed and motion direction control.The optimal caudal fin,whose topology is different from that of the natural fish caudal fin,make the 3D bionic fish achieve higher swimming efficiency,faster swimming speed and better maneuverability.The boundary vorticity-flux on the body surface of the 3D fish before and after optimization reveals the mechanism of high performance swimming of the topology optimization bionic fish.The comparative analysis between the swimming performance of the 3D topology optimization bionic fish and the 3D lunate tail bionic fish is also carried out,and the wake structures of two types of bionic fish show the physical nature that the swimming performance of the 3D topology optimization bionic fish is significantly better than the 3D lunate tail bionic fish.
基金supported by National Natural Science Foundation of China (Grant No. 50775109, Grant No. 51075201)Important Project of National Natural Science Foundation of China (Grant No. 50735002)Open Foundation for National Key laboratory of the Numerical Manufacturing Equipment and Technology of China (Grant No. DMETKF2009002)
文摘In the traditional flow-resistance-differential (FRD) type valve-less piezoelectric pump, the generated outflow and pressure are discontinuous because of the inherent periodicity and fluctuation of the pump. To overcome these drawbacks, utilizing the bending vibration of piezoelectric bimorph to drive fluid was conducted. However, our investigation on the current status of this piezoelectric bimorph pump shows that larger driving force and vibration amplitude are required for fluid pumping; the pumping can be realized through the centrifugal force; and the mechanism of fluid pumping is no longer further studied. Based on these cases, the paper designed a piezoelectric-stack pump with variable-cross-section oscillating (VCSO) vibrator by imitating the swing of the caudal-fin of tuna, and the pump is neither the rotating type nor the volumetric type according to the taxonomy. The interaction between the oscillating vibrator and the fluid parcel is firstly analyzed from the viewpoint of momentum conservation, and the analytical expression of pump flow rate is obtained. Then the modal and harmonic response analyses on the vibrator immerged in water are carried out. From the analyses the first two orders resonance frequencies are 832 Hz and 1 939 Hz, respectively, and the peak value of the tip amplitude is 0.6 mm. Laser Doppler vibrometer is used to measure both the frequency and vibration amplitude, and the determined first two orders resonance frequencies are 617 Hz and 1 356 Hz, respectively. The measured tip amplitude reaches to the peak value of 0.3 mm. At last, experimental measurement for the flow rates with different driving frequencies is conducted. The results show that the flow rate can reach 560 mL/min at 1 370 Hz when the pump runs under the backpressure of 30 mm water column. And the flow rate is as much as 560% of that of experiment results carried out by researchers from Brazil. The proposed pump innovates in both theory and taxonomy; in addition, the pump overcomes the drawbacks such as large flow fluctuation and low flow rate in the traditional FRD type pumps, which will help to broaden the application of the valve-less piezoelectric pump.