A cell line,termed ZFIN,was established from the caudal fin of zebrafish and was shown to be susceptible to spring viremia of carp virus(SVCV).The ZFIN cells are epithelial like cells and have a moderate plasmid trans...A cell line,termed ZFIN,was established from the caudal fin of zebrafish and was shown to be susceptible to spring viremia of carp virus(SVCV).The ZFIN cells are epithelial like cells and have a moderate plasmid transfection efficiency of 13.9%.Using an RNA-seq approach,differentially expressed genes(DEGs)regulated by SVCV were identified.Infection of SVCV gave rise to 3931 DEGs and up-regulated DEGs were mostly enriched into the biological regulation and cellular processes,among which pathways for the type I interferon signaling and the response to exogenous dsRNA were the top two GO terms.Several KEGG signaling pathways including TLR signaling pathway,RLR receptor signaling pathway,cytosolic DNA-sensing pathway,NLR signaling pathway,cytokine-cytokine receptor interaction and ferroptosis were significantly enriched.Antiviral genes including ifnφ1,isg15 and mx were significantly up-regulated.In addition,key DEGs involved in autophagy were identified.The results indicate that the ZFIN cell line provides a useful in vitro tool for study on the gene functions and cellular responses to viral infection in fish.展开更多
取欧洲鳗鲡(Anguilla anguilla)尾鳍进行体外培养,最终获得可连续传代的类纤维状的细胞系(欧洲鳗鲡尾鳍细胞系,European eel fin cell line,EEF)。探索不同传代比例对其生长增殖的影响。同时,以MTT法分析不同生长因子[碱性成纤维生长因...取欧洲鳗鲡(Anguilla anguilla)尾鳍进行体外培养,最终获得可连续传代的类纤维状的细胞系(欧洲鳗鲡尾鳍细胞系,European eel fin cell line,EEF)。探索不同传代比例对其生长增殖的影响。同时,以MTT法分析不同生长因子[碱性成纤维生长因子(FGF-basic)、肝细胞生长因子(HGF)、表皮生长因子(EGF)]对其增殖、形态的影响。结果显示:EEF增殖受上述生长因子调节作用很小,但是其形态却与FGF-basic的添加与否及其来源有很大关系。EEF细胞系的生长周期较目前所报道的欧洲鳗鲡细胞系快。染色体数目分析得出EEF的特征染色体数目为38条,这是首次基于体外培养细胞系的欧洲鳗鲡染色体数目分析。EEF的增殖、生长特性使之适宜于进一步开展鳗鲡病毒学、细胞生物学、基因组学、遗传学以及资源保护等方面的研究。展开更多
基金funded by the National Natural Science Foundation of China(Grant No.32030112 and U21A20268).
文摘A cell line,termed ZFIN,was established from the caudal fin of zebrafish and was shown to be susceptible to spring viremia of carp virus(SVCV).The ZFIN cells are epithelial like cells and have a moderate plasmid transfection efficiency of 13.9%.Using an RNA-seq approach,differentially expressed genes(DEGs)regulated by SVCV were identified.Infection of SVCV gave rise to 3931 DEGs and up-regulated DEGs were mostly enriched into the biological regulation and cellular processes,among which pathways for the type I interferon signaling and the response to exogenous dsRNA were the top two GO terms.Several KEGG signaling pathways including TLR signaling pathway,RLR receptor signaling pathway,cytosolic DNA-sensing pathway,NLR signaling pathway,cytokine-cytokine receptor interaction and ferroptosis were significantly enriched.Antiviral genes including ifnφ1,isg15 and mx were significantly up-regulated.In addition,key DEGs involved in autophagy were identified.The results indicate that the ZFIN cell line provides a useful in vitro tool for study on the gene functions and cellular responses to viral infection in fish.
文摘取欧洲鳗鲡(Anguilla anguilla)尾鳍进行体外培养,最终获得可连续传代的类纤维状的细胞系(欧洲鳗鲡尾鳍细胞系,European eel fin cell line,EEF)。探索不同传代比例对其生长增殖的影响。同时,以MTT法分析不同生长因子[碱性成纤维生长因子(FGF-basic)、肝细胞生长因子(HGF)、表皮生长因子(EGF)]对其增殖、形态的影响。结果显示:EEF增殖受上述生长因子调节作用很小,但是其形态却与FGF-basic的添加与否及其来源有很大关系。EEF细胞系的生长周期较目前所报道的欧洲鳗鲡细胞系快。染色体数目分析得出EEF的特征染色体数目为38条,这是首次基于体外培养细胞系的欧洲鳗鲡染色体数目分析。EEF的增殖、生长特性使之适宜于进一步开展鳗鲡病毒学、细胞生物学、基因组学、遗传学以及资源保护等方面的研究。
