Zebrafish hematopoietic stem and progenitor cells（HSPCs） originate from the hemogenic endothelium of the ventral wall of the dorsal aorta（DA） through the endothelial-to-hematopoietic transition（EHT） from approxi...Zebrafish hematopoietic stem and progenitor cells（HSPCs） originate from the hemogenic endothelium of the ventral wall of the dorsal aorta（DA） through the endothelial-to-hematopoietic transition（EHT） from approximately 30 to 60 hours post fertilization（hpf）. However, whether other artery sites can generate HSPCs de novo remains unclear. In this study, using live imaging and lineage tracing, we found that the caudal dorsal artery（CDA） in the caudal hematopoietic tissue directly gave rise to HSPCs through EHT.This process initiated from approximately 60 hpf and terminated at approximately 156 hpf. Compared with that in the DA, fewer EHT events were observed in the CDA. The EHT events in the DA and CDA were similarly regulated by Runx1 but differentially influenced by blood flow（i.e., the EHT frequency in CDA was affected to a lesser extent when circulation was compromised in the tnnt2a～（-/-）mutant）. Therefore,the whole artery, including both DA and CDA, was endowed with the ability to produce HSPCs during a much longer time period. Coincidently, the lineage tracing results indicated that adult hematopoietic cells originated from the embryonic endothelium, and those produced later preferentially colonized the adult thymus. Collectively, our study revealed that the CDA serves as an additional source of hematopoiesis, and it shows similar but not identical properties with the DA.展开更多
20111077 Chang Miman(Laboratory of Evolutionary Systematics of Vertebrates,Institute of Vertebrate Paleontology and Paleoanthropology,Chinese Academy of Sciences,Beijing 100044,China);Wang Ning Discovery of Cyclurus(A...20111077 Chang Miman(Laboratory of Evolutionary Systematics of Vertebrates,Institute of Vertebrate Paleontology and Paleoanthropology,Chinese Academy of Sciences,Beijing 100044,China);Wang Ning Discovery of Cyclurus(Amiinae,Amiidae,Amiiformes,Pisces)from China(Vertebrata Palasiatica,ISSN1000-3118,CN11-1905/Q,48(2),2010,p.85-100,6 illus.,1 table,52 refs.)Key words:Amiiformes,Hunan Province Described in this paper is a new amiine,Cyclurus orientalis,collected from the early to middle Eocene Xiawanpu Formation of Xiawanpu,Xiangxiang City,Hunan Province,China.It is unquestionably an amiine because of the diplospondylous vertebrae of both the normal and alternating types in its preural caudal region,the one-to-one fusion between the ural centra,except the first one,and the hypurals,the absence of the urodermals in the caudal skeleton,and the long dorsal fin.It is referred to Cyclurus because its first coronoid carries teeth with rounded tops.It is different from other species of Cyclurus in having fewer展开更多
基金supported by the National Natural Science Foundation of China (Nos.31571500,31771623 and 31771628)the National Key Basic Research Program of China (2015CB942802)+1 种基金the Fundamental Research Funds for the Central Universities (XDJK2017A015)the Guangdong Natural Science Fund for Distinguished Young Scholars (2017A030306024)
文摘Zebrafish hematopoietic stem and progenitor cells（HSPCs） originate from the hemogenic endothelium of the ventral wall of the dorsal aorta（DA） through the endothelial-to-hematopoietic transition（EHT） from approximately 30 to 60 hours post fertilization（hpf）. However, whether other artery sites can generate HSPCs de novo remains unclear. In this study, using live imaging and lineage tracing, we found that the caudal dorsal artery（CDA） in the caudal hematopoietic tissue directly gave rise to HSPCs through EHT.This process initiated from approximately 60 hpf and terminated at approximately 156 hpf. Compared with that in the DA, fewer EHT events were observed in the CDA. The EHT events in the DA and CDA were similarly regulated by Runx1 but differentially influenced by blood flow（i.e., the EHT frequency in CDA was affected to a lesser extent when circulation was compromised in the tnnt2a～（-/-）mutant）. Therefore,the whole artery, including both DA and CDA, was endowed with the ability to produce HSPCs during a much longer time period. Coincidently, the lineage tracing results indicated that adult hematopoietic cells originated from the embryonic endothelium, and those produced later preferentially colonized the adult thymus. Collectively, our study revealed that the CDA serves as an additional source of hematopoiesis, and it shows similar but not identical properties with the DA.
文摘20111077 Chang Miman(Laboratory of Evolutionary Systematics of Vertebrates,Institute of Vertebrate Paleontology and Paleoanthropology,Chinese Academy of Sciences,Beijing 100044,China);Wang Ning Discovery of Cyclurus(Amiinae,Amiidae,Amiiformes,Pisces)from China(Vertebrata Palasiatica,ISSN1000-3118,CN11-1905/Q,48(2),2010,p.85-100,6 illus.,1 table,52 refs.)Key words:Amiiformes,Hunan Province Described in this paper is a new amiine,Cyclurus orientalis,collected from the early to middle Eocene Xiawanpu Formation of Xiawanpu,Xiangxiang City,Hunan Province,China.It is unquestionably an amiine because of the diplospondylous vertebrae of both the normal and alternating types in its preural caudal region,the one-to-one fusion between the ural centra,except the first one,and the hypurals,the absence of the urodermals in the caudal skeleton,and the long dorsal fin.It is referred to Cyclurus because its first coronoid carries teeth with rounded tops.It is different from other species of Cyclurus in having fewer
