BACKGROUND Vitamin deficiencies are linked to various eye diseases,and the influence of vitamin D on cataract formation has been noted in prior research.However,detailed investigations into the causal relationship bet...BACKGROUND Vitamin deficiencies are linked to various eye diseases,and the influence of vitamin D on cataract formation has been noted in prior research.However,detailed investigations into the causal relationship between 25-(OH)D status and cataract development remain scarce.AIM To explore a possible causal link between cataracts and vitamin D.METHODS In this study,we explored the causal link between 25-(OH)D levels and cataract development using Mendelian randomization.Our analytical approach included inverse-variance weighting(IVW),MR-Egger,weighted median,simple mode,and weighted mode methods.The primary analyses utilized IVW with random effects,supplemented by sensitivity and heterogeneity tests using both IVW and MR-Egger.MR-Egger was also applied for pleiotropy testing.Additionally,a leave-one-out analysis helped identify potentially impactful single-nucleotide polymorphisms.RESULTS The analysis revealed a positive association between 25-(OH)D levels and the risk of developing cataracts(OR=1.11,95%CI:1.00-1.22;P=0.032).The heterogeneity test revealed that our IVW analysis exhibited minimal heterogeneity(P>0.05),and the pleiotropy test findings confirmed the absence of pleiotropy within our IVW analysis(P>0.05).Furthermore,a search of the human genotype-phenotype association database failed to identify any potentially relevant risk-factor single nucleotide polymorphisms.CONCLUSION There is a potential causal link between 25-(OH)D levels and the development of cataracts,suggesting that greater 25-(OH)D levels may be a contributing risk factor for cataract formation.Further experimental research is required to confirm these findings.展开更多
目的 探讨CD15抗原和组织蛋白酶D(Cath-D)在食管癌的表达意义及其相互关系。 方法 应用微波-LSAB免疫组化法,观察65例食管癌组织(男46例,女19例;平均年龄54岁;均为鳞癌)中CD15和Cath-D的表达阳性率及其相互关系。 结果 食管癌CD15和CD...目的 探讨CD15抗原和组织蛋白酶D(Cath-D)在食管癌的表达意义及其相互关系。 方法 应用微波-LSAB免疫组化法,观察65例食管癌组织(男46例,女19例;平均年龄54岁;均为鳞癌)中CD15和Cath-D的表达阳性率及其相互关系。 结果 食管癌CD15和CD阳性率分别为58％(38/65例)和65％(42/65例);食管癌CD15和Cath-D表达与性别、年龄、肿瘤部位、大体类型及肿瘤大小无关(P>0.05)。CD15和Cath-D表达阳性率:鳞癌Ⅲ级(88％,14/16 vs 100％,16/16)均显著高于鳞癌Ⅰ级(43％,6/14 vs 50％,7/14)和鳞癌Ⅱ级(51％,18/35 vs 54％,19/35,P<0.05);外膜层浸润(74％,20/27 vs82％,22/27)显著高于肌层浸润(47％,18/38 vs 53％,20/38,P<0.05);有淋巴结转移组(72％,26/36 vs 78％,28/38)显著高于无淋巴结转移组(41％,12/29 vs 48％、14/29,P<0.025);死亡组(70％,31/44 vs 77％,34/44)显著高于五年生存组(33％,7/21 vs 38％,8/21,P<0.01)。CD15阳性的肿瘤Cath-D阳性率显著高于CD15阴性者(82％,31/38 vs41％,11/27),CD15表达与Cath-D表达呈正相关(r=0.42,P<0.01)。 结论 检测CD15和Cath-D表达对判断食管癌恶性程度、预测其侵袭转移趋势和预后及指导治疗有重要意义。食管癌CD15与Cath-D表达具有相互协同作用可能。展开更多
文摘BACKGROUND Vitamin deficiencies are linked to various eye diseases,and the influence of vitamin D on cataract formation has been noted in prior research.However,detailed investigations into the causal relationship between 25-(OH)D status and cataract development remain scarce.AIM To explore a possible causal link between cataracts and vitamin D.METHODS In this study,we explored the causal link between 25-(OH)D levels and cataract development using Mendelian randomization.Our analytical approach included inverse-variance weighting(IVW),MR-Egger,weighted median,simple mode,and weighted mode methods.The primary analyses utilized IVW with random effects,supplemented by sensitivity and heterogeneity tests using both IVW and MR-Egger.MR-Egger was also applied for pleiotropy testing.Additionally,a leave-one-out analysis helped identify potentially impactful single-nucleotide polymorphisms.RESULTS The analysis revealed a positive association between 25-(OH)D levels and the risk of developing cataracts(OR=1.11,95%CI:1.00-1.22;P=0.032).The heterogeneity test revealed that our IVW analysis exhibited minimal heterogeneity(P>0.05),and the pleiotropy test findings confirmed the absence of pleiotropy within our IVW analysis(P>0.05).Furthermore,a search of the human genotype-phenotype association database failed to identify any potentially relevant risk-factor single nucleotide polymorphisms.CONCLUSION There is a potential causal link between 25-(OH)D levels and the development of cataracts,suggesting that greater 25-(OH)D levels may be a contributing risk factor for cataract formation.Further experimental research is required to confirm these findings.
文摘目的 探讨CD15抗原和组织蛋白酶D(Cath-D)在食管癌的表达意义及其相互关系。 方法 应用微波-LSAB免疫组化法,观察65例食管癌组织(男46例,女19例;平均年龄54岁;均为鳞癌)中CD15和Cath-D的表达阳性率及其相互关系。 结果 食管癌CD15和CD阳性率分别为58％(38/65例)和65％(42/65例);食管癌CD15和Cath-D表达与性别、年龄、肿瘤部位、大体类型及肿瘤大小无关(P>0.05)。CD15和Cath-D表达阳性率:鳞癌Ⅲ级(88％,14/16 vs 100％,16/16)均显著高于鳞癌Ⅰ级(43％,6/14 vs 50％,7/14)和鳞癌Ⅱ级(51％,18/35 vs 54％,19/35,P<0.05);外膜层浸润(74％,20/27 vs82％,22/27)显著高于肌层浸润(47％,18/38 vs 53％,20/38,P<0.05);有淋巴结转移组(72％,26/36 vs 78％,28/38)显著高于无淋巴结转移组(41％,12/29 vs 48％、14/29,P<0.025);死亡组(70％,31/44 vs 77％,34/44)显著高于五年生存组(33％,7/21 vs 38％,8/21,P<0.01)。CD15阳性的肿瘤Cath-D阳性率显著高于CD15阴性者(82％,31/38 vs41％,11/27),CD15表达与Cath-D表达呈正相关(r=0.42,P<0.01)。 结论 检测CD15和Cath-D表达对判断食管癌恶性程度、预测其侵袭转移趋势和预后及指导治疗有重要意义。食管癌CD15与Cath-D表达具有相互协同作用可能。
