[ Objective] To investigate the feasibility of the primary culture of bovine mammary epithelial cells in biochemical incubator. [ Method] In vitro, bovine mammary epithelial cells were isolated and cultured by the tis...[ Objective] To investigate the feasibility of the primary culture of bovine mammary epithelial cells in biochemical incubator. [ Method] In vitro, bovine mammary epithelial cells were isolated and cultured by the tissue explant method in order to investigate the optimal culture conditions. The morphology observation and identification of the cultured cells were performed by inverted microscope observation, Giemsa staining and cytokeratin immunohistochemistry. [ Result] Observed with inverted microscope, most of the bovine mammary epithelial cells were polygonal and displayed typical slabstone-like appearance. As it can be seen from cell staining results, the cell body was big and the nucleus was stained dark blue and was round or oval in shape, with clearly visible nucleoli, generally 2 -4 nucleoli. The tissue-specific expression of cytokeratin 14 and cytokeratin 18 genes in mammary epithelial cells was identified by cytokeratin immunohistochemistry. [ Conclusion] Primary bovine mammary epithelial cells were successfully cultured in biochemical incubator.展开更多
研究期刊认同的视觉修辞策略,旨在为学术期刊封面设计的改善提供参考依据。文章以视觉修辞中的认同观为理论视角,借助多模态话语分析,对国内顶级科技期刊Cell Research 2009—2018年的封面进行分析,并与国际期刊cell进行比较。封面设计...研究期刊认同的视觉修辞策略,旨在为学术期刊封面设计的改善提供参考依据。文章以视觉修辞中的认同观为理论视角,借助多模态话语分析,对国内顶级科技期刊Cell Research 2009—2018年的封面进行分析,并与国际期刊cell进行比较。封面设计者通过封面文章的人际功能、故事化图像的概念功能、排版的组篇功能以实现期刊的同情认同、无意识认同、对立认同。国内外顶级期刊都共同重视可视化图像的无意识认同策略,但在封面文章、版式设计方面有所差异。国内学术期刊在封面设计中应加强知识传播的可视化无意识认同修辞策略,并整合封面文章的人际功能和排版的组篇功能,引导受众进行系统性认知的心理认同建构。展开更多
Perovskite solar cells with TiO_2 electron transport layers exhibit power conversion efficiency(PCE) as high as 22.7% in single cells. However, the preparation process of the TiO_2 layer is adopted by an unscalable me...Perovskite solar cells with TiO_2 electron transport layers exhibit power conversion efficiency(PCE) as high as 22.7% in single cells. However, the preparation process of the TiO_2 layer is adopted by an unscalable method or requires high-temperature sintering, which precludes its potential use for mass production of flexible devices. In this study, a scalable low-temperature softcover-assisted hydrolysis(SAH) method is presented,where the precursor solution is sandwiched between a soft cover and preheated substrate to form a closed hydrolysis environment. Compact homogeneous TiO_2 films with a needle-like structure were obtained after the hydrolysis of a TiCl_4 aqueous solution. Moreover, by careful optimization of the TiO_2 fabrication conditions, a high PCE of 14.01% could be achieved for a solar module(4 × 4 cm^2) prepared using the SAH method. This method provides a novel approach for the efficient scale-up of the low-temperature TiO_2 film growth for industrial applications.展开更多
基金Supported by Natural Science Foundation of Inner Mongolia Autono-mous Region (200711020407)China Agricultural University and Inner Mongolia Agricultural University Cooperation Projects~~
文摘[ Objective] To investigate the feasibility of the primary culture of bovine mammary epithelial cells in biochemical incubator. [ Method] In vitro, bovine mammary epithelial cells were isolated and cultured by the tissue explant method in order to investigate the optimal culture conditions. The morphology observation and identification of the cultured cells were performed by inverted microscope observation, Giemsa staining and cytokeratin immunohistochemistry. [ Result] Observed with inverted microscope, most of the bovine mammary epithelial cells were polygonal and displayed typical slabstone-like appearance. As it can be seen from cell staining results, the cell body was big and the nucleus was stained dark blue and was round or oval in shape, with clearly visible nucleoli, generally 2 -4 nucleoli. The tissue-specific expression of cytokeratin 14 and cytokeratin 18 genes in mammary epithelial cells was identified by cytokeratin immunohistochemistry. [ Conclusion] Primary bovine mammary epithelial cells were successfully cultured in biochemical incubator.
文摘研究期刊认同的视觉修辞策略,旨在为学术期刊封面设计的改善提供参考依据。文章以视觉修辞中的认同观为理论视角,借助多模态话语分析,对国内顶级科技期刊Cell Research 2009—2018年的封面进行分析,并与国际期刊cell进行比较。封面设计者通过封面文章的人际功能、故事化图像的概念功能、排版的组篇功能以实现期刊的同情认同、无意识认同、对立认同。国内外顶级期刊都共同重视可视化图像的无意识认同策略,但在封面文章、版式设计方面有所差异。国内学术期刊在封面设计中应加强知识传播的可视化无意识认同修辞策略,并整合封面文章的人际功能和排版的组篇功能,引导受众进行系统性认知的心理认同建构。
基金supported financially by the National Natural Science Foundation of China (Grants Nos. 11574199, 11674219)the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning+1 种基金the Natural Science Foundation of Shanghai (17ZR1414800)the Baotou-SJTU innovation guidance fund Project (17H100000514)
文摘Perovskite solar cells with TiO_2 electron transport layers exhibit power conversion efficiency(PCE) as high as 22.7% in single cells. However, the preparation process of the TiO_2 layer is adopted by an unscalable method or requires high-temperature sintering, which precludes its potential use for mass production of flexible devices. In this study, a scalable low-temperature softcover-assisted hydrolysis(SAH) method is presented,where the precursor solution is sandwiched between a soft cover and preheated substrate to form a closed hydrolysis environment. Compact homogeneous TiO_2 films with a needle-like structure were obtained after the hydrolysis of a TiCl_4 aqueous solution. Moreover, by careful optimization of the TiO_2 fabrication conditions, a high PCE of 14.01% could be achieved for a solar module(4 × 4 cm^2) prepared using the SAH method. This method provides a novel approach for the efficient scale-up of the low-temperature TiO_2 film growth for industrial applications.
