Lethal effect of mononuclear cells derived from human umbilical cord blood differentiating into dendritic cells after in vitro induction of cytokines on neuroblastoma cells

BACKGROUND： Dendritic cell is the most major antigen presenting cell of organism. It is proved in recent studies that human umbilical cord blood mononuclear cells induced and cultured in vitro by recombinant human granulocyte-macrophage colony stimulating factor （rhG-MCSF） and recombinant human interleukin-4 （rhlL-4） can generate a great many dendritic cells and promote the lethal effect of T cells on human neuroblastoma, but it is unclear that whether the lethal effect is associated with the most proper concentration of dendritic cells. OBJEETIVE： To investigate the lethal effect of human umbilical cord blood mononuclear cells induced in vitro by cytokines differentiating into dendritic cells on human neuroblastoma, and its best concentration range. DESIGN ： Open experiment SEI-FING： Department of Pediatrics, the Medical School Hospital of Qingdao University MATERIALS ： The study was carried out in the Shandong Provincial Key Laboratory （Laboratory for the Department of Pediatrics of the Medical School Hospital of Qingdao University） during September 2005 to May 2006. Human umbilical cord blood samples were taken from the healthy newborn infants of full-term normal delivery during October to November 2005 in the Medical School Hospital of Qingdao University, and were voluntarily donated by the puerperas. Main instruments： type 3111 CO2 incubator （Forma Scientific, USA）, type 550 ELISA Reader （Bio-Rad, USA）. Main reagents： neuroblastoma cell line SK-N-SH （Shanghai Institute of Life Science, Chinese Academy of Sciences）, RPMI-1640 culture fluid and fetal bovine serum （Hyclone）, rhlL-4 （Promega, USA）, rhG-MCSF （Harbin Pharmaceutic Group Bioengineering Co.Ltd）, rat anti-human CDla monoclonal antibody and FITC-labeled rabbit anti-rat IgG （Xiehe Stem cell Gene Engineering Co.Ltd）. METHODS： ① Human umbilical cord blood mononuclear cells obtained with attachment methods differentiated into human umbilical cord blood dendritic cells, presenting typical morphology of dendritic cells after in vitro induction by rhG-MCSF and rhlL-4. ② Different concentrations of dendritic cells[ dendritic cells： neuroblastoma cells=20：1,50：1,100：1 （2×10^8 L^-1,5×10^8 L^-1,1×10^9 L^-1）], 1×10^9 L^-1 T cells and 1×10^7 L^-1 neuroblastoma cells were added in the experimental group. 1 ×10^9 L^-1 T cells and 1 ×10^7 L^-1 neuroblastoma cells were added in the control group. ③ Main surface marker CDla molecules of dendritic cells were detected with indirect immunofluorescence, and the percent rate of dendritic cells was counted with ultraviolet light and expressed as the expression rate of CD1a^＋ cells. ④Single effector cells and target cells were respectively set in the experimental group and control group to obtain the lethal effect. The lethal effect of dendritic cells on neuroblastoma cells was indirectly evaluated by detecting cellular survival with MTT assay. The lethal effect（%）= （1-A experimentat well-A effector cell /A target cell well）×100%.⑤The expenmental data were presented as Mean ±SD, and paired t test was used. MAIN OUTCOME MEASURES： ① Morphological characters of dendritic cells in the process of induction and differentiation. ②CD1a^＋ cellular expression rate. ③Lethal effect of dendntic cells on neuroblastoma cells. RESULTS： ①Morphological characters of dendritic cells in the process of induction and differentiation： On the 15^th day after human umbilical cord blood mononuclear cells were induced by rhG-MCSF and rhlL-4, typical morphology of dendritic cells could be seen under an inverted microscope. ②Expression rate of CD1a^＋ cells was （43.12±5.83）%. ③Lethal effect of dendritic cells on neuroblastoma cells： Lethal effect of dendritic cells stimulated T cells in each experimental group （ dendritic cells： neuroblastoma cells=100：1,50：1, 20：1 respectively） on neuroblastoma cells was significantly higher than that in control group[（31.00 ±4.41 ）%, （30.92±5.27）%,（33.57±5.35）%,（26.23±5.20）%, t=3.51,2.98,4.24, P〈 0.01 ）; But the lethal effect of dendntic cells on neuroblastoma was significantly lower when their ratio was 100：1 and 50：1 in comparison with 20：1 （t=2.01,2.36, P 〈 0.05）, and no significant difference in lethal effect existed between the ratio at 100：1 and 50：1 （t=0.06,P 〉 0.05）. CONCLUSION： Dendritic cells differentiated from human umbilical cord blood mononuclear cells after in vitro induction of cytokines can promote the lethal effect of T cells on neuroblastoma cells. The lethal effect is associated with the concentration of dendritic cells within some range.

Numerical Simulation of Azimuthal Uniformity of Injection Currents in Single-Point-Feed Induction Voltage Adders

In order to investigate the injection current uniformity around the induction cell bores, two fully electromagnetic （EM） models are respectively established for a single-stage induction cell and an induction voltage adder （IVA） with three cells stacked in series, without considering electron emission. By means of these two models, some factors affecting the injection current uni- formity are simulated and analyzed, such as the impedances of adders and loads, cell locations, and feed timing of parallel driving pulses. Simulation results indicate that higher impedances of adder and loads are slightly beneficial to improve injection current uniformity. As the impedances of adder and loads increase from 5 Ω to 30Ω, the asymmetric coefficient of feed currents decreases from 10.3% to 6.6%. The current non-uniformity within the first cell is a little worse than that in other downstream cells. Simulation results also show that the feed timing would greatly affect current waveforms, and consequently cause some distortion in pulse fronts of cell output voltages. For a given driving pulse with duration time of 70-80 ns, the feed timing with a time deviation of less than 20 ns is acceptable for the three-cell IVAs, just causing the rise time of output voltages to increase about 5 ns at most and making the peak voltage decrease by 3.5%.

An Attempt to Analyze a Human Nervous System Algorithm for Sensing Earthquake Precursors

We statistically validate the 2011-2022 earthquake prediction records of Ada, the sixth finalist of the 2nd China AETA in 2021, who made 147 earthquake predictions (including 60% of magnitude 5.5 earthquakes) with a prediction accuracy higher than 70% and a confidence level of 95% over a 12-year period. Since the reliable earthquake precursor signals described by Ada and the characteristics of Alfvén waves match quite well, this paper proposes a hypothesis on how earthquakes are triggered based on the Alfvén (Q G) torsional wave model of Gillette et al. When the plume of the upper mantle column intrudes into the magma and lithosphere of the soft flow layer during the exchange of hot and cold molten material masses deep inside the Earth’s interior during ascent and descent, it is possible to form body and surface plasma sheets under certain conditions to form Alfven nonlinear isolated waves, and Alfven waves often perturb the geomagnetic field, releasing huge heat and kinetic energy thus triggering earthquakes. To explain the complex phenomenon of how Ada senses Alvfen waves and how to locate epicenters, we venture to speculate that special magnetosensory cells in a few human bodies can sense earthquake precursors and attempt to hypothesize an algorithm that analyzes how the human biological nervous system encodes and decodes earthquake precursors and explains how human magnetosensory cells can solve complex problems such as predicting earthquake magnitude and locating epicenters.

Magnetic transition of ferromagnetic material at high pressure using a novel system

A system for the investigation of the magnetic properties of materials under high pressure is fabricated based on diamond anvil cell （DAC） technology. The system is designed with an improved coil arranged around the diamond of a non-magnetic DAC. Using this system, the magnetic transition of ferromagnetic （Fe） sample under increasing pressure can be observed. We successfully obtain the evolution of magnetic properties as a function of applied pressure reaching 26.9 GPa in the Fe sample. A magnetic transition is observed at approximately 13 GPa, which is consistent with the theoretical prediction.