Objective: To observe and compare the effect of traditional Chinese medicine (TCM) combined with chemotherapy (CT) on immune function and quality of life (QOL)of patients with non-small cell lung cancer (NSCLC) in sta...Objective: To observe and compare the effect of traditional Chinese medicine (TCM) combined with chemotherapy (CT) on immune function and quality of life (QOL)of patients with non-small cell lung cancer (NSCLC) in stage Ⅲ-Ⅳ. Methods: One hundred cases with stage Ⅲ-Ⅳ NSCLC were randomly divided into two groups. The treated group (n=50) received CT combined with TCM, and the control group received CT alone. The percentage of T lymphocyte subset in peripheral blood and the change of natural killer (NK) cell count were observed after treatment. The QOL and tolerance of CT were also compared between the two groups after treatment. Results: In the treated group, CD3 cell count, CD4 cell count, CD4/ CDg ratio and NK cell activity were higher than those in control group, while CD8 cell count in the treated group was lower than that in the control group (P<0.05), and QOL and tolerance of CT in the treated group were also better (P<0.05). Conclusion: TCM combined with CT could raise the patients' ability in tolerating CT in stage Ⅲ-ⅣNSCLC.展开更多
Objective： To explore the effect and mechanism of ginsenoside Rg3 （Shenyi Capsule, 参一胶囊） on the postoperative life span of patients with non-small cell lung cancer （NSCLC）. Methods： The prospective, randomiz...Objective： To explore the effect and mechanism of ginsenoside Rg3 （Shenyi Capsule, 参一胶囊） on the postoperative life span of patients with non-small cell lung cancer （NSCLC）. Methods： The prospective, randomized, controlled method was adopted. One hundred and thirty- three patients with NSCLC were randomly assigned to 3 groups： Shenyi Capsule group （43 cases）, combined therapy group （Shenyi Capsule plus chemotherapy, 46 cases）, and chemotherapy group （44 cases）. The survival rates, immune function and the correlation between vascular endothelial growth factor （VEGF） expression and clinical effect were analyzed in the three groups. Results： （1） The 1-year survival rate in the Shenyi group, the combined group and the chemotherapy group was 76.7% （33/43）, 82.6% （38/46）, and 79.5% （35/44）, respectively; the 2-year survival rate was 67.4% （29/43）, 71.7% （33/46）, and 70.5% （31/44）, respectively; and the 3-year survival rate was 46.5% （20/43）, 54.3% （25/46）, and 47.7% （21/44）, respectively. There was no significant difference among the 3 groups （P〉0.05）. （2） NK cells were increased to different degrees and the ratio of CD4/CD8 was normal in the Shenyi Capsule group and the combined group, while the ratio of CD4/CD8 was disproportional in the chemotherapy group. （3） In the chemotherapy group, the 3-year survival rate was lower in patients with positive expression of VEGF than in patients with negative expression （37.0% vs 64.7%, χ^2=17.9, P〈0.01）, but no significant statistical difference was shown in the other two groups （53.6% vs 55.6%, P〉0.05; 44.4% vs 50.0%, P〉0.05）. Conclusion： Shenyi Capsule, especially in combination with chemotherapy, can improve the life span of patients with NSCLC after operation. The mechanism might be correlated with improving the immune function and anti-tumor angiogenesis.展开更多
Objective: To explore the mechanism ofintegrated traditional Chinese and Westernmedicine (TCM--WM ) therapy on chronicaplastic anemia (CAA). Methods: The RBClife span of 30 normal human subjects and 30patients with CA...Objective: To explore the mechanism ofintegrated traditional Chinese and Westernmedicine (TCM--WM ) therapy on chronicaplastic anemia (CAA). Methods: The RBClife span of 30 normal human subjects and 30patients with CAA were measured by sir labelled technique before and after TCM--WMtherapy. The morphology and distribution ofRBC membrane protein granules were observed by freeze fracture etching and transmission electron microscope. Results: The halflife of erythrocytes (RBC TI/2)was shortenedin CAA cases and there was a significant difference compared to healthy control (P <0. 01). After therapy, the RBC life span prolonged and approached the normal level. Before treatment, there existed abnormal in morphology, decrease in amount and uneven indistribution of protein granules in protoplasmicface (PF) and extracellular face (EF) of RBCmembrane. After treatment, the protein granules of RBC membrane was improved and approached to control. Conclusions: The morphology, amount, quality and distribution ofRBC membrane protein granule were closelyrelated to its life span. The therapeutic effectof TCM--WM was better than that of WMalone and it had a function both in stabilizingmembrane protein and extending the RBC lifespan.展开更多
目的 寻求使处于生长停滞期的人脐静脉内皮细胞 (HUVEC)增殖寿命延长的方法。 方法 将体外转录质粒 pc DNA3- h TERT用 Bam HI酶切使之线性化 ,经 T7RNA聚合酶催化以转录合成 h TERT m RNA。用脂质体法将 h TERT m RNA导入处于生长...目的 寻求使处于生长停滞期的人脐静脉内皮细胞 (HUVEC)增殖寿命延长的方法。 方法 将体外转录质粒 pc DNA3- h TERT用 Bam HI酶切使之线性化 ,经 T7RNA聚合酶催化以转录合成 h TERT m RNA。用脂质体法将 h TERT m RNA导入处于生长停滞期的 HUVEC。检测 h TERT m RNA导入后细胞端粒酶活性表达与细胞生长增殖情况。 结果 导入 h TERT m RNA后 ,细胞有端粒酶活性的暂时表达 ,增殖寿命较单纯用脂质体处理的对照细胞延长了 7代。 结论 h TERT m RNA导入细胞能可控地、瞬时地重建细胞端粒酶活性 ,可望广泛应用于人体细胞增殖寿命延长的尝试。展开更多
Objective To study the effects of diallyl sulfide (DAS), an organosulfur compoundpresent in garlic (Allium sativum), on the life span of ehrlich ascites (EA) tumor bearingSwiss albino mice, cytotoxicity and angiog...Objective To study the effects of diallyl sulfide (DAS), an organosulfur compoundpresent in garlic (Allium sativum), on the life span of ehrlich ascites (EA) tumor bearingSwiss albino mice, cytotoxicity and angiogenesis. Methods EA tumor cells weremaintained by serial transplantation in peritoneal cavity of male Swiss albino mice. EAtumor cells were inoculated at concentrations of 1× 106 EA cells, 2.5× 106 EA cells and5× 106 EA cells. DAS was given in 0.2 ml normal saline i. p., daily for seven days followedone hour later by inoculation with EA cells in respective groups. Results The resultsrevealed that administration of DAS increased the life span of EA tumor bearing animals bymore than 25 percent. A significant dose dependant cytotoxic response of DAS was alsoobserved on EA tumor cells. DAS was also found to inhibit the angiogenesis in EA tumorbearing mice in a dose dependent manner. Conclusion It is suggested that DAS may exertits anticarcinogenic effects by more than one mechanism and is a useful chemopreventiveand chemotherapeutic agent.展开更多
文摘Objective: To observe and compare the effect of traditional Chinese medicine (TCM) combined with chemotherapy (CT) on immune function and quality of life (QOL)of patients with non-small cell lung cancer (NSCLC) in stage Ⅲ-Ⅳ. Methods: One hundred cases with stage Ⅲ-Ⅳ NSCLC were randomly divided into two groups. The treated group (n=50) received CT combined with TCM, and the control group received CT alone. The percentage of T lymphocyte subset in peripheral blood and the change of natural killer (NK) cell count were observed after treatment. The QOL and tolerance of CT were also compared between the two groups after treatment. Results: In the treated group, CD3 cell count, CD4 cell count, CD4/ CDg ratio and NK cell activity were higher than those in control group, while CD8 cell count in the treated group was lower than that in the control group (P<0.05), and QOL and tolerance of CT in the treated group were also better (P<0.05). Conclusion: TCM combined with CT could raise the patients' ability in tolerating CT in stage Ⅲ-ⅣNSCLC.
文摘Objective： To explore the effect and mechanism of ginsenoside Rg3 （Shenyi Capsule, 参一胶囊） on the postoperative life span of patients with non-small cell lung cancer （NSCLC）. Methods： The prospective, randomized, controlled method was adopted. One hundred and thirty- three patients with NSCLC were randomly assigned to 3 groups： Shenyi Capsule group （43 cases）, combined therapy group （Shenyi Capsule plus chemotherapy, 46 cases）, and chemotherapy group （44 cases）. The survival rates, immune function and the correlation between vascular endothelial growth factor （VEGF） expression and clinical effect were analyzed in the three groups. Results： （1） The 1-year survival rate in the Shenyi group, the combined group and the chemotherapy group was 76.7% （33/43）, 82.6% （38/46）, and 79.5% （35/44）, respectively; the 2-year survival rate was 67.4% （29/43）, 71.7% （33/46）, and 70.5% （31/44）, respectively; and the 3-year survival rate was 46.5% （20/43）, 54.3% （25/46）, and 47.7% （21/44）, respectively. There was no significant difference among the 3 groups （P〉0.05）. （2） NK cells were increased to different degrees and the ratio of CD4/CD8 was normal in the Shenyi Capsule group and the combined group, while the ratio of CD4/CD8 was disproportional in the chemotherapy group. （3） In the chemotherapy group, the 3-year survival rate was lower in patients with positive expression of VEGF than in patients with negative expression （37.0% vs 64.7%, χ^2=17.9, P〈0.01）, but no significant statistical difference was shown in the other two groups （53.6% vs 55.6%, P〉0.05; 44.4% vs 50.0%, P〉0.05）. Conclusion： Shenyi Capsule, especially in combination with chemotherapy, can improve the life span of patients with NSCLC after operation. The mechanism might be correlated with improving the immune function and anti-tumor angiogenesis.
文摘Objective: To explore the mechanism ofintegrated traditional Chinese and Westernmedicine (TCM--WM ) therapy on chronicaplastic anemia (CAA). Methods: The RBClife span of 30 normal human subjects and 30patients with CAA were measured by sir labelled technique before and after TCM--WMtherapy. The morphology and distribution ofRBC membrane protein granules were observed by freeze fracture etching and transmission electron microscope. Results: The halflife of erythrocytes (RBC TI/2)was shortenedin CAA cases and there was a significant difference compared to healthy control (P <0. 01). After therapy, the RBC life span prolonged and approached the normal level. Before treatment, there existed abnormal in morphology, decrease in amount and uneven indistribution of protein granules in protoplasmicface (PF) and extracellular face (EF) of RBCmembrane. After treatment, the protein granules of RBC membrane was improved and approached to control. Conclusions: The morphology, amount, quality and distribution ofRBC membrane protein granule were closelyrelated to its life span. The therapeutic effectof TCM--WM was better than that of WMalone and it had a function both in stabilizingmembrane protein and extending the RBC lifespan.
文摘目的 寻求使处于生长停滞期的人脐静脉内皮细胞 (HUVEC)增殖寿命延长的方法。 方法 将体外转录质粒 pc DNA3- h TERT用 Bam HI酶切使之线性化 ,经 T7RNA聚合酶催化以转录合成 h TERT m RNA。用脂质体法将 h TERT m RNA导入处于生长停滞期的 HUVEC。检测 h TERT m RNA导入后细胞端粒酶活性表达与细胞生长增殖情况。 结果 导入 h TERT m RNA后 ,细胞有端粒酶活性的暂时表达 ,增殖寿命较单纯用脂质体处理的对照细胞延长了 7代。 结论 h TERT m RNA导入细胞能可控地、瞬时地重建细胞端粒酶活性 ,可望广泛应用于人体细胞增殖寿命延长的尝试。
文摘Objective To study the effects of diallyl sulfide (DAS), an organosulfur compoundpresent in garlic (Allium sativum), on the life span of ehrlich ascites (EA) tumor bearingSwiss albino mice, cytotoxicity and angiogenesis. Methods EA tumor cells weremaintained by serial transplantation in peritoneal cavity of male Swiss albino mice. EAtumor cells were inoculated at concentrations of 1× 106 EA cells, 2.5× 106 EA cells and5× 106 EA cells. DAS was given in 0.2 ml normal saline i. p., daily for seven days followedone hour later by inoculation with EA cells in respective groups. Results The resultsrevealed that administration of DAS increased the life span of EA tumor bearing animals bymore than 25 percent. A significant dose dependant cytotoxic response of DAS was alsoobserved on EA tumor cells. DAS was also found to inhibit the angiogenesis in EA tumorbearing mice in a dose dependent manner. Conclusion It is suggested that DAS may exertits anticarcinogenic effects by more than one mechanism and is a useful chemopreventiveand chemotherapeutic agent.
