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Single-cell RNA sequencing analysis of the retina under acute high intraocular pressure
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作者 Shaojun Wang Siti Tong +5 位作者 Xin Jin Na Li Pingxiu Dang Yang Sui Ying Liu Dajiang Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第11期2522-2531,共10页
High intraocular pressure causes retinal ganglion cell injury in primary and secondary glaucoma diseases,yet the molecular landscape characteristics of retinal cells under high intraocular pressure remain unknown.Rat ... High intraocular pressure causes retinal ganglion cell injury in primary and secondary glaucoma diseases,yet the molecular landscape characteristics of retinal cells under high intraocular pressure remain unknown.Rat models of acute hypertension ocular pressure were established by injection of cross-linked hyaluronic acid hydrogel(Healaflow■).Single-cell RNA sequencing was then used to describe the cellular composition and molecular profile of the retina following high intraocular pressure.Our results identified a total of 12 cell types,namely retinal pigment epithelial cells,rod-photoreceptor cells,bipolar cells,Müller cells,microglia,cone-photoreceptor cells,retinal ganglion cells,endothelial cells,retinal progenitor cells,oligodendrocytes,pericytes,and fibroblasts.The single-cell RNA sequencing analysis of the retina under acute high intraocular pressure revealed obvious changes in the proportions of various retinal cells,with ganglion cells decreased by 23%.Hematoxylin and eosin staining and TUNEL staining confirmed the damage to retinal ganglion cells under high intraocular pressure.We extracted data from retinal ganglion cells and analyzed the retinal ganglion cell cluster with the most distinct expression.We found upregulation of the B3gat2 gene,which is associated with neuronal migration and adhesion,and downregulation of the Tsc22d gene,which participates in inhibition of inflammation.This study is the first to reveal molecular changes and intercellular interactions in the retina under high intraocular pressure.These data contribute to understanding of the molecular mechanism of retinal injury induced by high intraocular pressure and will benefit the development of novel therapies. 展开更多
关键词 APOPTOSIS axon degeneration high intraocular pressure MICROGLIA ocular hypertension photoreceptor cells RETINA retinal degeneration retinal ganglion cells single-cell RNA sequencing
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Casein kinase-2 inhibition promotes retinal ganglion cell survival after acute intraocular pressure elevation
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作者 Meng Wang Shi-Qi Yao +8 位作者 Yao Huang Jia-Jian Liang Yanxuan Xu Shaowan Chen Yuhang Wang Tsz Kin Ng Wai Kit Chu Qi Cui Ling-Ping Cen 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第5期1112-1118,共7页
Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2... Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2 inhibition can promote retinal ganglion cell survival and axonal regeneration in rats after optic nerve injury.To investigate the underlying mechanism,in the current study we increased the intraocular pressure of adult rats to 75 mmHg for 2 hours and then administered a casein kinase-2 inhibitor(4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole)by intravitreal injection.We found that intravitreal injection of 4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole promoted retinal ganglion cell survival and reduced the number of infiltrating macrophages.Transcriptomic analysis showed that the mitogen activated protein kinase signaling pathway was involved in the response to intraocular pressure elevation but was not modulated by the casein kinase-2 inhibitors.Furthermore,casein kinase-2 inhibition downregulated the expression of genes(Cck,Htrsa,Nef1,Htrlb,Prph,Chat,Slc18a3,Slc5a7,Scn1b,Crybb2,Tsga10ip,and Vstm21)involved in intraocular pressure elevation.Our data indicate that inhibition of casein kinase-2 can enhance retinal ganglion cell survival in rats after acute intraocular pressure elevation via macrophage inactivation. 展开更多
关键词 casein kinase-2 GLAUCOMA intraocular pressure elevation MACROPHAGES retinal ganglion cells
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ULTRASTRUCTURE OF THE VEGETATIVE CELLS OF NOSTOC FLAGELLIFORME PREPARED WITH HIGH PRESSURE FREEZING AND FREEZE SUBSTITUTION TECHNIQUE 被引量:8
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作者 祝建 王俊 +1 位作者 华振基 马丁.米勒 《Acta Botanica Sinica》 CSCD 1998年第10期901-905,共5页
The ultrastructure of the vegetative cells of Nostoc flagelliforme Born. et Flah. was investigated with high pressure freezing and freeze substitution technique and compared with the results obtained by using conv... The ultrastructure of the vegetative cells of Nostoc flagelliforme Born. et Flah. was investigated with high pressure freezing and freeze substitution technique and compared with the results obtained by using conventional preparation methods. During the processes of chemical fixation, dehydration and embedding, the cell structures might be more artificially modified than that obtained from high pressure freezing and freeze substitution. With the present method, the sheath of N. flagelliforme could be well penetrated and no extra big space could exist between the cell and the sheath. The cell protoplasm rarely shrinked. Some fine structures of cell inclusions and unit membranes became visualized. Many bacteria were harbored in the sheath. In addition, the presence of big vacuoles in the cell of N. flagelliforme as well as the presence of bacteria in the sheath shown in the present preparation for cyanobacteria has not been described so far in the literature. 展开更多
关键词 ULTRASTRUCTURE Nostoc flagelliforme Vegetative cell High pressure freezing Freeze substitution
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Cold atmospheric-pressure air plasma treatment of C6 glioma cells:effects of reactive oxygen species in the medium produced by the plasma on cell death 被引量:3
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作者 Yuyang WANG Cheng CHENG +5 位作者 Peng GAO Shaopeng LI Jie SHEN Yan LAN Yongqiang YU Paul K CHU 《Plasma Science and Technology》 SCIE EI CAS CSCD 2017年第2期63-68,共6页
An atmospheric-pressure air plasma is employed to treat C6 glioma cells in vitro. To elucidate on the mechanism causing cell death and role of reactive species (RS) in the medium produced by the plasma, the concentr... An atmospheric-pressure air plasma is employed to treat C6 glioma cells in vitro. To elucidate on the mechanism causing cell death and role of reactive species (RS) in the medium produced by the plasma, the concentration of the long-lived RS such as hydrogen peroxide, nitrate, and ozone in the plasma-treated liquid (phosphate-buffered saline solution) is measured. When vitamin C is added to the medium as a ROS quencher, the viability of C6 glioma cells after the plasma treatment is different from that without vitamin C. The results demonstrate that reactive oxygen species (ROS) such as H2O2, and O3 constitute the main factors for inactivation of C6 glioma cells and the reactive nitrogen species (RNS) may only play an auxiliary role in cell death. 展开更多
关键词 atmospheric pressure air plasma reactive species cell death
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Use of casing and its effect on pressure cells 被引量:4
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作者 AHANGARI K NOORZAD A 《Mining Science and Technology》 EI CAS 2010年第3期384-390,共7页
Although pressure cells have been produced and installed successfully for decades,the accuracy of measured pressure is often inadequate.Due to large differences between the stiffness of pressure cells and the surround... Although pressure cells have been produced and installed successfully for decades,the accuracy of measured pressure is often inadequate.Due to large differences between the stiffness of pressure cells and the surrounding media,there is a considerable difference between applied pressure and that measured from pressure cells.It is often difficult and expensive to make a pressure cell with stiffness(modulus of elasticity) similar to the surrounding material in which it will be embedded.In order to improve this situation,a casing material with proportional dimensions is recommended as a means to obtain reliable results.In our study,the effect of using casing in the installation of pressure cells is investigated,providing the characteristics of casing.Some practical recommendations are presented to improve the accuracy of the results using casing. 展开更多
关键词 INSTRUMENTATION stress measurement pressure cell CASING
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Time Course of Age-dependent Changes in Intraocular Pressure and Retinal Ganglion Cell Death in DBA/2J Mouse 被引量:4
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作者 Marcel Y. Avila Alan M. Laties 《眼科学报》 2006年第3期184-189,194,共7页
Purpose:To characterizes the progression of glaucoma in DBA/2J mice by measuring intraocular pressure(IOP) and retinal ganglion cells(RGCs) numbers in mice of various ages. Methods:A quantitative assessment of the pat... Purpose:To characterizes the progression of glaucoma in DBA/2J mice by measuring intraocular pressure(IOP) and retinal ganglion cells(RGCs) numbers in mice of various ages. Methods:A quantitative assessment of the pathophysiology of the DBA/2J mice was performed and the C57/BL6 mice was used as control. The IOP was measured by the servo-null micropipette system; the regional patterns of the loss of RGCs were determined by cell count of retrogradely-labeled RGCs. Results:The baseline IOP for DBA/2J mice at 7 weeks was (16.6 ± 1.2)mm Hg.Then IOP increased extend to 12 months, with the peak of (25.2 ± 1.2)mm Hg at 6 months of age. Retinal ganglion cell numbers did not decrease relative to control until 12 months of age(P=0.006), when the loss was proportionally higher in peripheral regions(P<0.05). Conclusion:The elevation in IOP precedes the loss of RGCs by several months. RGCs cell loss occurs particularly in peripheral regions of the retina. These findings expand our understanding of the changes in DBA/2J mice and provide information for experiments design when they are used as a glaucoma model for future studies of RGCs degeneration in glaucoma. 展开更多
关键词 GLAUCOMA DBA/2J Mouse Intraocular pressure Retinal ganglion cell.
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Autologous mesenchymal stem cells applied on the pressure ulcers had produced a surprising outcome in a severe case of neuromyelitis optica 被引量:1
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作者 Adriana Octaviana Dulamea Mirela-Patricia Sirbu-Boeti +4 位作者 Coralia Bleotu Denisa Dragu Lucia Moldovan Ioana Lupescu Giancarlo Comi 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第11期1841-1845,共5页
Recent studies provided evidence that mesenchymal stem cells(MSCs) have regenerative potential in cutaneous repair and profound immunomodulatory properties making them a candidate for therapy of neuroimmunologic dis... Recent studies provided evidence that mesenchymal stem cells(MSCs) have regenerative potential in cutaneous repair and profound immunomodulatory properties making them a candidate for therapy of neuroimmunologic diseases. Neuromyelitis optica(NMO) is an autoimmune, demyelinating central nervous system disorder characterized by a longitudinally extensive spinal cord lesion. A 46-year-old male diagnosed with NMO had relapses with paraplegia despite treatment and developed two stage IV pressure ulcers(PUs) on his legs. The patient consented for local application of autologous MSCs on PUs. MSCs isolated from the patient's bone marrow aspirate were multiplied in vitro during three passages and embedded in a tridimensional collagen-rich matrix which was applied on the PUs. Eight days after MSCs application the patient showed a progressive healing of PUs and improvement of disability. Two months later the patient was able to walk 20 m with bilateral assistance and one year later he started to walk without assistance. For 76 months the patient had no relapse and no adverse event was reported. The original method of local application of autologous BM-MSCs contributed to healing of PUs. For 6 years the patient was free of relapses and showed an improvement of disability. The association of cutaneous repair, sustained remission of NMO and improvement of disability might be explained by a promotion/optimization of recovery mechanisms in the central nervous system even if alternative hypothesis should be considered. Further studies are needed to assess the safety and efficacy of mesenchymal stem cells in NMO treatment. 展开更多
关键词 neuromyelitis optica Devic's syndrome mesenchymal stem cells multiple sclerosis pressure ulcers
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Comparative study on pressure swing adsorption system for industrial hydrogen and fuel cell hydrogen 被引量:2
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作者 Jian Chen Lingbing Bu Yingqi Luo 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2022年第2期112-119,共8页
In order to improve the design of PSA system for fuel cell hydrogen production,a non-isothermal model of eight-bed PSA hydrogen process with five-component(H_(2)/N_(2)/CH_(4)/CO/CO_(2)=74.59%/0.01%/4.2%/2.5%/18.7%(vol... In order to improve the design of PSA system for fuel cell hydrogen production,a non-isothermal model of eight-bed PSA hydrogen process with five-component(H_(2)/N_(2)/CH_(4)/CO/CO_(2)=74.59%/0.01%/4.2%/2.5%/18.7%(vol))four-stage pressure equalization was developed in this article.The model adopts a composite adsorption bed of activated carbon and zeolite 5 A.In this article,pressure variation,temperature field and separation performance are stimulated,and also effect of providing purge(PP)differential pressure and the ratio of activated carbon to zeolite 5 A on separation performance in the process of producing industrial hydrogen(CO content in hydrogen is 10μl·L^(-1))and fuel cell hydrogen(CO content is 0.2μl·L^(-1))are compared.The results show that Run 3,when the CO content in hydrogen is 10μl·L^(-1),the hydrogen recovery is 89.8%,and the average flow rate of feed gas is 0.529 mol·s^(-1);When the CO content in hydrogen is 0.2μl·L^(-1),the hydrogen recovery is 85.2%,and the average flow rate of feed gas is 0.43 mol·s^(-1).With the increase of PP differential pressure,hydrogen recovery first increases and then decreases,reaching the maximum when PP differential pressure is 0.263 MPa;With the decrease of the ratio of activated carbon to zeolite 5 A,the hydrogen recovery increases gradually.When the CO content in hydrogen is 0.2μl·L^(-1) the hydrogen recovery increases more obviously,from 83.96%to 86.37%,until the ratio of activated carbon to zeolite 5 A decreases to 1.At the end of PP step,no large amount of CO_(2) in gas or solid phase enters the zeolite 5 A adsorption bed,while when the CO content in hydrogen is 10μl·L^(-1),and the ratio of carbon to zeolite 5 A is less than 1.4,more CO_(2) will enter the zeolite 5 A bed. 展开更多
关键词 pressure swing adsorption HYDROGEN Fuel cell hydrogen Industrial hydrogen Numerical simulation
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Increased production of recombinant prourokinase with porous microcarrier cell culture by periodic pressure oscillation in a stirred tank reactor 被引量:3
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作者 胡显文 Gao Lihua +2 位作者 Li Zuohu Xiao Chengzu Xu Zhaoping 《High Technology Letters》 EI CAS 2006年第3期311-317,共7页
An rCHO cell line expressing recombinant human prourokinase (pro-UK) at the level of 5μg/ 10^6cells/d was cultivated on Cytopore cellulose porous microcarriers in a 7.5L Biostat CT stirred tank reactor. A periodic ... An rCHO cell line expressing recombinant human prourokinase (pro-UK) at the level of 5μg/ 10^6cells/d was cultivated on Cytopore cellulose porous microcarriers in a 7.5L Biostat CT stirred tank reactor. A periodic pressure oscillation of 0.04 MPa and 0.04 Hz was adopted to introduce a physical stimulus on the rCHO cells and to improve mass transfer characteristic between cells and medium in the process of porous microcarrier CHO cell culture. Compared to constant pressure culture, the oscillation culture didn't influence specific cell growth rate significantly, but could enhance the pro-UK specific production by 10% - 40%, and reduce production of lactate by 10% - 30%. In the perfusion culture of recombinant CHO cell with serum-free medium for 67 days, cell density could reach 2.64×10^7/ml, the maximal prourokinase concentration in harvested supernatant was about 118mg/L, a total of 21.1 grams of prourokinase was produced in 313 liters of supernatant. In conclusion, the perfusion cell culture with periodic pressure oscillation can enhance the production of recombinant protein and increase the reactor specific productivity. 展开更多
关键词 cell culture porous microcarrier PROUROKINASE periodic pressure oscillation
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Protective effects of nerve regeneration factor and brain-derived neurotrophic factor on retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure 被引量:1
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作者 Zhengru Huang Huaijin Guan +1 位作者 Fei Ding Xiaosong Gu 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第6期445-449,共5页
BACKGROUND: Previous studies have shown that nerve regeneration factor (NRF) provides neuroprotective effects. However, the neuroprotective effects on retinal ganglion cells in an animal model of glaucoma remain un... BACKGROUND: Previous studies have shown that nerve regeneration factor (NRF) provides neuroprotective effects. However, the neuroprotective effects on retinal ganglion cells in an animal model of glaucoma remain uncertain. OBJECTIVE: To determine the neuroprotective effects of NRF on retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure and to compare the effects on brain-derived neurotrophic factor (BDNF). DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at Jiangsu Provincial Key Laboratory of Neural Regeneration from September 2006 to August 2007. MATERIALS: Sterone, a major component of NRF, was provided by the Key Laboratory of Neural Regeneration, Nantong University in China; BDNF was provided by BioDesign Inc., USA. METHODS: A total of 24 healthy rabbits were randomly assigned to NRF, BDNF, and phosphate buffered saline groups, with 8 rabbits per group. The left eyes were considered normal controls, and acute hyper-intraocular pressure was induced in the right eyes via anterior chamber perfusion. The right camera vitrea bulbi was injected with 4.5 μg NRF, 3.75 μg BDNF, or 5 μL 0.1 mol/L phosphate buffered saline, respectively. MAIN OUTCOME MEASURES: Retinal ganglion cells were reverse-labeled using horseradish peroxidase to quantify cell density at 2, 4, and 6 mm from the optic disc edge. RESULTS: NRF increased the number of surviving retinal ganglion cells at the optic disc edge (P 〈 0.01 or P 〈 0.05). The density of surviving retinal ganglion cells decreased with increasing distance from the optic disc. The number of retinal ganglion cells in the BDNF group was similar to the NRF group (P 〉 0.05). At 2, 4, and 6 mm away from the optic disc edge, there was no significant difference in retinal ganglion cell density between NRF and BDNF groups (P〉 0.05). CONCLUSION: NRF provided protection to retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure, Le., NRF enhanced the survival rate of retinal ganglion cells. The neuroprotective effect was similar to BDNF. 展开更多
关键词 nerve regeneration factor brain-derived neurotrophic factor retinal ganglion cells hyper-intraocular pressure NEUROPROTECTION nerve factor neural regeneration
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Neutron Diffraction of Large-Volume Samples at High Pressure Using Compact Opposed-Anvil Cells 被引量:1
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作者 Xiao-Lin Ni Lei-Ming Fang +3 位作者 Xin Li Xi-Ping Chen Lei Duan-Wei He Zi-Li Kou 《Chinese Physics Letters》 SCIE CAS CSCD 2018年第4期12-15,共4页
Neutron diffraction techniques of large-volume samples at high pressure using compact opposed-anvil cells are developed at a reactor neutron source, China's Mianyang research reactor. We achieve a high-pressure condi... Neutron diffraction techniques of large-volume samples at high pressure using compact opposed-anvil cells are developed at a reactor neutron source, China's Mianyang research reactor. We achieve a high-pressure condition of in situ neutron diffraction by means of a newly designed large-volume opposed-anvil cell. This pressure calibration is based on resistance measurements of bismuth and the neutron diffraction of iron. Pressure calibration experiments are performed at room temperature for a new cell using the tungsten carbide anvils with a tapered angle of 30°, Φ4.5 mm culet diameter and the metal-nonmetal composite gasket with a thickness of 2 mm. Transitions in Bi(Ⅰ–Ⅱ 2.55 GPa, Ⅱ–V 7.7 GPa) are observed at 100 and 300 kN, respectively, by resistance measurements.The pressure measurement results of neutron diffraction are consistent with resistance measurements of bismuth.As a result, pressures up to about 7.7 GPa can routinely and stably be achieved using this apparatus, with the sample volume of 9 mm^3. 展开更多
关键词 Neutron Diffraction of Large-Volume Samples at High pressure Using Compact Opposed-Anvil cells
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Advanced high-pressure plasma diagnostics with hairpin resonator probe surrounded by film and sheath 被引量:1
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作者 徐金洲 石建军 +3 位作者 张菁 张琪 中村圭二 菅井秀郎 《Chinese Physics B》 SCIE EI CAS CSCD 2010年第7期387-393,共7页
The hairpin probe using microwave resonance in plasma is applicable to high pressure 1.33 ×10^3-1.01×10^5 Pa)) as developed recently. In this work, an analytic model of the hairpin resonator probe surround... The hairpin probe using microwave resonance in plasma is applicable to high pressure 1.33 ×10^3-1.01×10^5 Pa)) as developed recently. In this work, an analytic model of the hairpin resonator probe surrounded by a thin dielectric layer and a sheath layer is proposed. The correction factor due to these surroundings is analytically found and confirmed by electromagnetic field finite difference time domain simulation, thus enabling the accurate measurement of electron density in a high-pressure non-equilibrium uniform discharge. 展开更多
关键词 hairpin resonator probe simulation high-pressure plasma transmission line model
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Cubic anvil cell apparatus for high-pressure and low-temperature physical property measurements 被引量:3
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作者 Jin-Guang Cheng Bo-Sen Wang +1 位作者 Jian-Ping Sun Yoshiya Uwatoko 《Chinese Physics B》 SCIE EI CAS CSCD 2018年第7期100-106,共7页
We will build a cubic anvil cell (CAC) apparatus for high-pressure and low-temperature physical property measurements in the synergic extreme condition user facility (SECUF). In this article, we first introduce th... We will build a cubic anvil cell (CAC) apparatus for high-pressure and low-temperature physical property measurements in the synergic extreme condition user facility (SECUF). In this article, we first introduce the operating principle, the development history, and the current status of the CAC apparatus, and subsequently describe the design plan and technical targets for the CAC in SECUF. We will demonstrate the unique advantages of CAC, i.e., excellent pressure homogeneity and large hydrostatic pressure capacity, by summarizing our recent research progresses using CAC. Finally, we conclude by providing some perspectives on the applications of CAC in the related research fields. 展开更多
关键词 cubic anvil cell high-pressure measurements temperature-pressure phase diagram strongly cor-related electron systems
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Amine-containing film deposited in pulsed dielectric barrier discharge at a high pressure and its cell adsorption behaviours
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作者 胡文娟 谢芳艳 +1 位作者 陈强 翁静 《Chinese Physics B》 SCIE EI CAS CSCD 2009年第3期1276-1282,共7页
With monomer allylamine, amine-containing functional films were prepared in alternative current pulsed dielectric barrier discharge (DBD) at a high pressure. This paper analyses in detail the film properties and str... With monomer allylamine, amine-containing functional films were prepared in alternative current pulsed dielectric barrier discharge (DBD) at a high pressure. This paper analyses in detail the film properties and structures, such as hydrophilicity, compounds and microstructures as well as amine density by the water contact angle, Fourier transform infrared spectroscopy, atomic force microscopy, and ultraviolet-visible measurement. The influence of discharge param- eters, in particular applied power, on amine density was investigated. As an application the cell adsorption behaviours on plasma polymerization films was performed in-vitro. The results show that at a high pressure pulsed DBD plasma can polymerize films with sufficient amine group on surface, through which the very efficient cell adsorption behaviours was demonstrated, and the high rate of cell proliferation was visualized. 展开更多
关键词 pulsed DBD plasma high pressure ALLYLAMINE cell adsorption behaviour
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Effects of Hydrostatic Pressure on Cultured Bovine Trabecular Meshwork Cells
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作者 姜发纲 魏厚仁 +2 位作者 吕源淑 周元清 张缨 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1999年第4期319-322,327,共5页
In order to explore the effects of pressure on trabecular meshwork cells, bovine trabecular meshwork cells were cultured in vitro and subjected to different levels of hydrostatic pressure. The cellular morphology, ult... In order to explore the effects of pressure on trabecular meshwork cells, bovine trabecular meshwork cells were cultured in vitro and subjected to different levels of hydrostatic pressure. The cellular morphology, ultrastructure and phagocytosis were studied with inverted phase-contrast microscopy, light microscopy and transmission electron microscopy, etc. It was found that the cells subjected to 2. 0 kPa or 2. 67 kPa for 48 h had no remarkable difference as compared with the controls in terms of parameters observed. Those under 4. 0 kPa for 24 h showed slight changes in structure and a mild decrease in phagocytic function. The damage appeared more severe if the pressure was higher or lasted longer. From the above we conclude that trabecular meshwork cells can only bear pressure below a certain level. They may be destroyed structurally or impaired functionally by pressure over this level. 展开更多
关键词 trabecular meshwork cells pressure GLAUCOMA
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Study of the structure and architecture of aortic smooth muscle cells under pressure overloading in rats
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作者 张放鸣 何光篪 +1 位作者 刘正津 陈海平 《Journal of Medical Colleges of PLA(China)》 CAS 1995年第2期87-92,共6页
Male Wistar rats were used to study the changes of the structure and architecture of the smooth muscle cells(SMCs) of the aorta under pressure overloading(PO).The aorta was cut open longitudinally and the tunica media... Male Wistar rats were used to study the changes of the structure and architecture of the smooth muscle cells(SMCs) of the aorta under pressure overloading(PO).The aorta was cut open longitudinally and the tunica media was examined with a histological tech 展开更多
关键词 AORTA SMOOTH MUSCLE cell ARCHITECTURE strucrture pressure OVERLOADING rat
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A fluorescence turn-on Hg<sup>2+</sup>probe based on rhodamine with excellent sensitivity and selectivity in living cells
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作者 Li Jiang Ling Wang +2 位作者 Bo Zhang Gui Yin Rui-Yong Wang 《Open Journal of Inorganic Chemistry》 2011年第2期16-22,共7页
A highly sensitive and selective Hg2+ probe 4 based on rhodamine-b was developed and characterized. In consideration of environmental and biological application, we connected a water soluble receptor group (sulfonated... A highly sensitive and selective Hg2+ probe 4 based on rhodamine-b was developed and characterized. In consideration of environmental and biological application, we connected a water soluble receptor group (sulfonated β-naphtol) and rhodamine-b together through hydrazine hydrate in high yield. The result turns out that this compound not only exhibits excellent sensitivity and selectivity toward Hg2+, but also shows well cell permeability and compatibility in vitro. 展开更多
关键词 FLUORESCENCE probe Mercury RHODAMINE Yeast HELA cell
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Pressure shift mediated anoikis of endothelial cells in the flow field in vitro
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作者 Jia Hu Er-Yong Zhang +4 位作者 Jiang Wu Wei-Lin Xu Huai-Qinq Chen Ying-Kang Shi Ying-Qiang Guo 《Journal of Biomedical Science and Engineering》 2010年第2期206-212,共7页
Dramatic changes of pressure in the local circulation flow field would lead to alterations in biorheological characteristics of Endothelial cells(ECs), and futher resulted in the apoptosis induced by loss of anchorage... Dramatic changes of pressure in the local circulation flow field would lead to alterations in biorheological characteristics of Endothelial cells(ECs), and futher resulted in the apoptosis induced by loss of anchorage, a form of cell death known as anoikis. In this study, we set levels of pressure(negative and positive pressure) loaded ECs groups and non-activated cultured ECs ,single shear stress loaded ECs as control group to demonstrate the effects of pressure shift on cell morphogenesis and adhesion. Furthermore, we investigate the effects of pressure shift on ECs proli- feration and apoptosis to elucidate the influences of pressure shift on vitality of ECs. We present these data here to suggest that the negative pressure might be another important factor beyond velocity and shear stress in biomechanical impairment on ECs, then to trigger the apoptosis with the extracellular matrix (ECM) detachment (anoikis). As the negative pressure is thought to play a role in the anoikis process, these results have implications for both the path- ogenesis and therapeutics investigations of stenostic vessel diseases and the future vascular tissue engineering. 展开更多
关键词 ENDOTHELIAL cells ANOIKIS cell ADHESION pressure Flow Field
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A Novel Low Air Pressure-Assisted Approach for the Construction of Cells-Decellularized Tendon Scaffold Complex
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作者 Xin HUANG Zheng-tao LV +1 位作者 Peng CHENG An-min CHEN 《Current Medical Science》 SCIE CAS 2022年第3期569-576,共8页
Objective The goal of this study was to develop a decellularized tendon scaffold(DTS)and repopulate it with adipose-derived stem cells(ADSCs)assisted by low air pressure(LP).Methods The porcine superficial flexor tend... Objective The goal of this study was to develop a decellularized tendon scaffold(DTS)and repopulate it with adipose-derived stem cells(ADSCs)assisted by low air pressure(LP).Methods The porcine superficial flexor tendons were processed into the DTSs using a combination of physical,chemical,and enzymatic treatments.The effectiveness of decellularization was verified by histological analysis and DNA quantification.The properties of the DTSs were evaluated by quantitative analysis of biochemical characterization,porosimetry,in vitro biocompatibility assessment,and biomechanical testing.Subsequently,the ADSCs-DTS complexes were constructed via cell injection assisted by LP or under atmospheric pressure.The differences in cell distribution,biomechanical properties,and the total DNA content were compared by histological analysis,biomechanical testing,and DNA quantification,respectively.Results Histological analysis confirmed that no cells or condensed nuclear materials were retained within the DTSs with widened interfibrillar space.The decellularization treatment resulted in a significant decrease in the content of DNA and glycosaminoglycans,and a significant increase in the porosity.The DTSs were cytocompatible in vitro and did not show reduced collagen content and inferior biomechanical properties compared with the fresh-frozen tendons.The assistance of LP promoted the broader distribution of cells into the adjacent interfibrillar space and cell proliferation in DTSs.The biomechanical properties of the scaffolds were not significantly affected by the recellularization treatments.Conclusion A novel LP-assisted approach for the construction of cells-DTS complex was established,which could be a methodological foundation for further bioreactor and in vitro studies. 展开更多
关键词 TENDON DEcellULARIZATION adipose-derived stem cells recellularization low air pressure tissue engineering
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Influence of Working Pressure on Ion Sensitive Probe Measurement in Microwave ECR Plasmas
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作者 马志斌 吴俊 +3 位作者 谭必松 沈武林 潘鑫 汪建华 《Plasma Science and Technology》 SCIE EI CAS CSCD 2015年第4期294-297,共4页
In order to precisely measure the ion parameters in a microwave electron cyclotron resonance plasma using an ion sensitive probe,the dependences of the current-voltage(I-V)characteristics on the shielding height(h... In order to precisely measure the ion parameters in a microwave electron cyclotron resonance plasma using an ion sensitive probe,the dependences of the current-voltage(I-V)characteristics on the shielding height(h)and the potential difference between inner and outer electrodes(V_B)have been investigated at different working pressures of 0.03 Pa and 0.8 Pa.Results show that the I-V curves at higher pressure are more sensitive to the variation of h than those at lower pressure.The influence of V_B on ion temperature(T_i)measurement becomes more prominent when the pressure is increased from 0.03 Pa to 0.8 Pa.Under both pressures,the optimized h is obtained at the condition where the current reaches zero in the positive voltage region with a suitable V_B of-1.5 V because of effective shielding of the electron E×B drift. 展开更多
关键词 working pressure ion sensitive probe ion temperature plasma
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