The Mesenchymal stem cell regulates the immune system: secretion of cytokines, cell-to-cell contact and extracellular vesicles

MSCs are pluripotent stem cells which have multipotent differentiation potential and immunosuppressive ability.Therefore,MSCs play an important role in regenerative medicine;the study of their mechanisms of regulating immune responses will also lead to new strategies for the treatment of immune-inflammatory diseases.Studies have shown that MSCs can modulate adaptive and innate immunity by secreting cytokines or cell-to-cell contact and play an immunosuppressive role.In addition,the secretion of extracellular vesicles by MSCs is also an important immunomodulatory mechanism.MSCs need to be cultured in vitro before they can be used to modulate immunity.It has been shown that pretreatment of MSCs during culture can improve cell survival rate and further enhance the ability of immunomodulation.In this paper,the mechanism of MSCs regulating immune response is discussed to deepen the understanding of MSCs regulating immunity.

Changing the balance of the MTO reaction dual-cycle mechanism: Reactions over ZSM-5 with varying contact times

The methanol to olefins （MTO） reaction was performed over ZSM‐5 zeolite at 300℃ under various methanol weight hourly space velocity （WHSV） values. During these trials, the catalytic perfor‐mance was assessed, in addition to the formation and function of organic compounds retained in the zeolite. Analysis of reaction effluents and confined organics demonstrated a dual‐cycle reaction mechanism when employing ZSM‐5. The extent of the hydrogen transfer reaction, a secondary reac‐tion in the MTO process, varied as the catalyst‐methanol contact time was changed. In addition, 12C/13C‐methanol switch experiments indicated a relationship between the dual‐cycle mechanism and the extent of the hydrogen transfer reaction. Reactions employing a low methanol WHSV in conjunction with a long contact time favored the hydrogen transfer reaction to give alkene products and promoted the generation and accumulation of retained organic species, such as aromatics and methylcyclopentadienes, which enhance the aromatic cycle. When using higher WHSV values, the reduced contact times lessened the extent of the hydrogen transfer reaction and limited the genera‐tion of methylcyclopentadienes and aromatic species. This suppressed the aromatic cycle, such that the alkene cycle became the dominant route during the MTO reaction.

Contact Mechanism of the Ag-doped Trimolybdate Nanowire as An Antimicrobial Agent

Antibacterial Ag-agents are intensively applied as broad spectrum, high-stability, high-efficiency and high-safety inorganic antibacterial agents. We have developed a new kind of antibacterial Ag-agent, namely Ag_2-x(NH_4)xMo_3O_(10) ·3H_2O nanowires(NWs). Carrying Ag atoms in the lattice and Ag-rich nanoparticles on the surface, the Ag-doped NWs show strong antibacterial effects for a variety of bacteria including E.coli, Staphylococcus aureus, Candida albicans and Aspergil lus niger. By performing systematic comparison experiments, we have proven that the main antibacterial effects are neither resulted from the tiny amount of Ag+ions released from the Ag-doped NWs in aqueous solutions, nor resulted from Ag-rich nanoparticles of fragments of the NWs when they are slowly dissolved in the Martin broth. Instead, the effects are mainly resulted from a contact mechanism, under which, the Ag-doped NWs need to be physically in contact with the bacteria to be eliminated. This is a novel phenomenon observed in the interactions between nanomaterials and live cells, which is worthy of further investigation at the molecular scale. As the Ag-doped NWs are not dissolved in pure water or weak acids, one may find practical antibacterial applications in textile industry and food storage industry for these unique nanomaterials.

Effect of contact angle and helix angle on slide-roll ratio under the accelerated motion state of ball screw mechanism

To study the effect of the contact angle and helix angle on slide-roll ratio at the ball contact points under the accelerated motion state of ball screw mechanisrm(B S M),the curve theory in differential geometry a d the homogeneous transformation matrix ae used to establish the acceleration kinematics model of BSM.The model can be used to describe the accelerated motion relationships among the screw,balls and nut,calculate the acceleration of relative motion at the contact points between the balls and raceways,and analyze five accelerated motion rules between the balls and raceways.It also conducts a simulation analysis of the slide-roll ratio relationship between the accelerations at the ball center and the contact point of ball under different contact angles and helix angles.As shownby the analysis,with the increase in the BSM’s contact angle,the slide-roll ratio at the contact points decreases,and the contact angle has a relatively significant effect on the slide-roll ratio.However,with the decrease in the BSM’helix angle,the slide-roll ratio at the contact points decreases,and the helix angle has a relatively insignificant effect on the slide-roll ratio.By measuring the accelerations of both the screw and nut under the accelerated motion state,it also verifies the existence of the slide-roll mixed motion at the ball contact point A between the ball and the screw racewayand pure rolling at the ball contact point B between the ball and the nut raceway during the accelerated motion.

Friction contact mechanisms of layered surface

In this paper,we firstly review the carbon layered surface prepared with electron cyclotron resonance (ECR) plasma sputtering. Secondly,the friction behavior of carbon layered surface under pin-on-disk testing is described. Furthermore,the contact stress evolution processes of layered surface with and without transfer layer during wear are given for understanding the contact mechanisms. Finally,a three-dimension (3D) local yield map of layered surface is introduced,which is useful to predict the possible contact mechanisms.

Tribological behavior and wear mechanism of resin-matrix contact strip against copper with electrical current

The resin-matrix pantograph contact strip (RMPCS),which has excellent abrasion resistance with electrical current and friction-reducing function,was developed in view of the traditional contact strips with high maintenance cost,high wear rate with electrical current and severe damage to the copper conducting wire.The characteristics of worn surfaces,cross-section and typical elemental distributions of RMPCS were studied by scanning electron microscopy (SEM) and energy dispersion spectrometry (EDS).The wear behavior and arc discharge of RMPCS against copper were investigated with self-made electrical wear tester.The results show that the electrical current plays a critical role in determining the wear behavior,and the wear rate of the RMPCS against copper with electrical current is 2.7-5.8 times higher than the value without electrical current.The wear rate of the contact strip increases with the increase of the sliding speed and electrical current density.The main wear mechanism of RMPCS against copper without electrical current is low stress grain abrasive and slightly adhesive wear,while arc erosion wear and oxidation wear are the dominate mechanism with electrical current,which is accompanied by adhesive wear during the process of wear.

EPS-contact-leaching mechanism of chalcopyrite concentrates by A.ferrooxidans

extracelluar 的效果黄铜矿面对氧化铁、氧化硫的细菌集中的 bioleaching 上的聚合物质(EPS )( 一。ferrooxidans ) 被学习。Fe2+and Cu2+ 离子的细菌的数字， pH，氧化还原作用潜力，和集中被调查。沥滤的残余被 X 光检查衍射和英尺红外分析。结果显示 EPS 让细菌容易遵守黄铜矿表面，它对在不利环境的细菌有用。同时，有 Fe3+ 的 EPS 电影层在黄铜矿的表面上扔并且成为氧转移的一个障碍到黄铜矿使钝化黄铜矿，并且通过集中 Fe3+ 离子加速 bioleaching 创造高氧化还原作用潜力空间在黄铜矿的黄铁矿专注。结果建议那 EPS 形成支持 bioleaching 黄铁矿并且禁止 bioleaching 黄铜矿，特别在高潜在的状况下面。

Study on Contact Fatigue Life and Failure Mechanism of Subquenched 42CrMo Steel

The effect of undissolved ferrite amount in subcritically quenched 42CrMo steel on contact fatigue properties and failure mechanism were studied. The amount of undissolved ferrite in the steel were 0%,3%,10%,15% and 20% in volume fraction, respectively. The experimental results show that the existence of undissolved ferrite can increase the contact fatigue life The contact fatigue life can be prolonged with increasing the amounts of undissolved ferrite The grain size can be fined by using subcritical quenching process and the area of phase boundaries can also be greatly increased. The stress relaxation and grain refinement due to occurring of plastic deformation are main reasons for improving the fatigue life. The existence of undissolved ferrite can increase the crack initiation period. Under the experiment conditions, when the amount of undissolved ferrite is 10%, the longest contact fatigue life can be the obtained.

Analytical modeling and approaches of multihelix cables incorporating with interwire mutual contacts

This study aims to develop an analytical model based on the curve beam theory to capture the mechanical response of a multihelix cable considering the internal contact displacements.Accordingly,a double-helix cable subjected to axial tension and torsion is analyzed,and both the line and point contacts between the neighboring wires and strands are considered via an equivalent homogenized approach.Then,the proposed theoretical model is extended to a hierarchical multihelix cable with mutual contact displacements by constructing a recursive relationship between the high-and low-level multihelix structures.The global tensile stiffness and torsional stiffness of the double-helix cable are successfully evaluated.The results are validated by a finite element(FE)model,and are found to be consistent with the findings of previous studies.It is shown that the contact deformations in multihelix cables significantly affect their equivalent mechanical stiffness,and the contact displacements are remarkably enhanced as the helix angles increase.This study provides insights into the interwire/interstrand mutual contact effects on global and local responses.

Preparation and Anti⁃arc Erosion Property of Ag Matrix Electrical Contact Material Reinforced by Novel Ti_(2)Cd

Due to their outstanding electrical contact properties,Cd-containing silver-matrix electrical contact materials can meet the requirements of high stability and long life for military defense and aerospace applications.In order to further reduce the Cd content under the premise of meeting the high-performance requirements,in this study,high-purity intermediate Ti_(2)Cd powder of MAX phase(Ti_(2)CdC)was synthesized with a pressureless technique and then applied to reinforce the Ag matrix.The Cd content of the as-prepared Ag/Ti_(2)Cd composites was actually reduced by 38.31%compared with conventional Ag/CdO material.Based on the systematic study of the effect of heat treatment temperature on the physical phase,morphology,interface and comprehensive physical properties of Ag/Ti_(2)Cd composites,the preferred samples(heat treated at 400°C for 1 h)showed high density(97.77%),low resistivity(2.34μΩ·cm),moderate hardness(90.8HV),high tensile strength(189.9 MPa),and exhibited good electrical contact performance after 40000 cycles of arc discharging under severe conditions(DC 28 V/20 A).The results of microscopic morphological evolution,phase change and elemental distribution of the electrical contact surface show that the combination of high stability of Ti_(2)Cd reinforcing phase,good interfacial bonding with Ag matrix and improved melt pool viscosity in the primary stage of arc erosion,results in low and stable contact resistance(average value 13.20 mΩ)and welding force(average value 0.6 N),low fluctuation of static force(2.2-2.5 N).The decomposition and absorption energy of Ti_(2)Cd and the arc extinguishing effect of Cd vapor are the main reasons for the stable arcing energy and arcing time of electric contacts in the late stage of arc erosion.

Mechanism of Ti/Al/Ni/Au ohmic contacts to AlGaN/GaN heterostructures via laser annealing

The physical mechanisms of Ti/Al/Ni/Au ohmic contacts to AlGaN/GaN heterostructures by laser annealing and rapid thermal annealing are systematically investigated. The microstructures indicate that a better surface morphology and an intact contact interface are formed after laser annealing. None of the TiN alloy spikes are formed at the interface of the laser annealing sample. The experimental results show that the current transport mechanism through the ohmic contact after laser annealing is different from the conventional spike mechanism, and it is dominated by thermionic field emission.

INFILTRATION MECHANISM AND QUALITY CONTROL OF CONTACT MATERIALS

The infiltration mechanism, which has great significance for the quality control of electrieal contact material made from W-Cu, W-Ag alloys with high content of tungsten, has been studied. And a directive infiltration technology for improving the product quality and gaining a better economic benefit has been developed.

Source-Contacting Gas: Accumulation Mechanism and Distribution in China

Source-contacting gas, which is also called basin-center gas, deep basin gas, is the tight-sand gas accumulation contacting closely to its source rocks. Having different accumulation mechanisms from conventional gas reservoirs that are formed by replacement way, the typical source-contacting gas reservoirs are formed by piston-typed migration forward way. Source-contacting gas accumulations exhibit a series of distinctly mechanic characteristics. According to the valid combination of these characteristics, the estimation for the type of discovered gas reservoirs or distributions of source-contacting gas reservoirs can be forecasted. The source-contacting gas is special for having no edge water or bottom water for gas and complicated gas-water relationships, which emphasizes the intimate association of reservoir rocks with source rocks, which is called the root of the gas reservoir. There are many basins having the mechanic conditions for source-contacting gas accumulations in China, which can be divided into three regions. Most of the basins with favorable accumulation conditions are located mainly in the central and western China. According to the present data, basins having source-contacting gas accumulations in China can be divided into three types, accumulation conditions and configuration relationships are the best in type A basins and they are the larger basins in central China. Type B basins with plain accumulation conditions exist primarily in eastern China and also the basins in western China. Accumulation conditions and exploration futures are worse in type C basins, which refer mainly to the small basins in southern China and China Sea basins. Main source-contacting gas basins in China are thoroughly discussed in this paper and the distribution patterns of source-contacting gas in five huge basins are discussed and forecasted.

The mechanisms of cracks formation of silver-based contact materials under arc and making pressure

The arc erosion experiments on five kinds of silver-based contact materials, AgZnO (10), AgSnO2 (8.5) In2O3 (4), AgCdO (12), AgNi(10), AgWC(12)C(3) were carried out according to different breaking times, breaking currents, and making pressures. Then based on the theoretical analysis and the photographs taken by scanning electronic mi-croscope with EDAX analyzer, the crack morphology was studied scientifically. Three types of cracks, which are cavity cracks, grain boundaries (or phase boundaries) slipping cracks, and thermal stress cracks, were put forward under arc and making pressure through establishing their physical models and discussing their formation mechanisms.

Immunology Mechanism of CD4^＋ CD25^ T Regulatory Cells Acting on Effector T Cells

Objective:To detect the inhibiting co-stimulating molecule CTLA4 and cytokines secreted by Treg cells, and explore the immunology mechanism of T regulatory cells acting on effector T cells in co-cultured system(CCS) and separating-cultured system(SCS). Methods: Detecting the percentage of CTLA4 and CD28 expressed on the Treg cells and effector T cells, and then adding Treg cells to mixed lymphocyte reaction(MLR) system in CCS and TransWell Millicell-PCF SCS, at the same time, adding or not adding anti-IL-10 or anti-TGF-β1 to the reacting systems, examining the inhibitory capacity of Treg cells exerting on the MLR. Results: Compared with effector T cells, Treg cells expressed higher level CTLA4 and secreted much more IL-10 and TGF-β1(P<0.01). The inhibitory capacity of Treg cells co-cultured with effector T cells is much stronger than that in separating cultured group(P<0.01). Moreover, the inhibiting rate of Treg cells exerting on effector T cells through secreting IL-10 was more powerful than that through secreting TGF-β1(P<0.01). Conclusion: Both cell-to-cell contact and cytokines secretion mechanisms are involved in CD4 +CD25 + Treg cells operating function. However, the former is more important. Intrestingly, we for the first time point found that IL-10 plays more powerful roles than TGF-β1 in the cytokines secretion mechanism.

Finite Element Analysis of the Contact Mechanics of Ceramic-on-Ceramic Hip Resurfacing Prostheses

Ceramics are good alternative to metal as bearing couple materials because of their better wear resistance. A Finite Element(FE) study was performed to investigate the contact mechanics and stress distribution of Ceramic-on-Ceramic (COC) hip resurfacingprostheses. It was focused in particular on a parametric study to examine the effects of radial clearance, loading,alumina coating on the implants, bone quality, and fixation of cup-bone interface. It was found that a reduction in the radialclearance had the most significant effect on the predicted contact pressure distribution among all of the parameters considered inthis study. It was determined that there was a significant influence of non-metallic materials, such as the bone underneath thebearing components, on the predicted contact mechanics. Stress shielding within the bone tissue was found to be a major concernwhen regarding the use of ceramic as an alternative to metallic resurfacing prostheses. Therefore, using alumina implantswith a metal backing was found to be the best design for ceramic resurfacing prostheses in this study. The loading, bone quality,and acetabular cup fixation conditions were found to have only minor effects on the predicted contact pressure distribution alongthe bearing surfaces.

Three-dimensional parametric contact analysis of planetary roller screw mechanism and its application in grouping for selective assembly

The planetary roller screw mechanism(PRSM)is a novel precision transmission mechanism that realizes the conversion between linear and rotary motions.The contact characteristics of helical surfaces directly determine PRSM’s performance in load-carrying capacity and transmission accuracy.Therefore,studying the contact characteristics of PRSM forms the fundamental basis for enhancing its transmission performance.In this study,a three-dimensional parametric analysis method of contact characteristics is proposed based on the PRSM meshing principle and PyVista(a high-level API to the Visualization Toolkit).The proposed method considers the influence of machining errors among various thread teeth.The effects of key machining errors on contact positions and axial clearance,as well as their sensitivities,are analyzed.With excellent solution accuracy,this method exhibits higher calculation efficiency and stronger robustness than the analytical and numerical meshing models.The influence of nominal diameter and pitch errors of the screw,roller,and nut on the axial clearance follows a linear relationship,whereas flank angle errors have negligible effects on the axial clearance.The corresponding influence coefficients for these three machining errors on the axial clearance are 0.623,0.341,and 0.036.The variations in contact positions caused by individual errors are axisymmetric.Flank angle errors and roller diameter errors result in linear displacements of the contact points,whereas pitch errors cause the contact points to move along the arc of the roller diameter.Based on the proposed threedimensional parametric contact characteristics analysis method,the Fuzzy C-Means clustering algorithm considering error sensitivity is utilized to establish a component grouping technique in the selective assembly of critical PRSM components,ensuring the rational and consistent clearances based on the given component’s machining errors.This study provides effective guidance for analyzing contact characteristics and grouping in selective assembly for PRSM components.It also presents the proposed method’s potential applicability to similar calculation problems for contact positions and clearances in other transmission systems.

Sedimentation of short cylindrical pollutants with mechanical contacts

Lattice Boltzmann method and elastic particle collision model were used to investigate the sedimentation of short cylindrical pollutant particles with mechanical contacts for varying bulk number density ε and terminal Reynolds number ReT. The corresponding experiments were performed as a comparison. The clusters of pollutant particles with an inverted “T” structure were observed, the pollutant particles for high ε and large ReT scattered wider than that for low ε and small ReT. The sedimentation velocities increased suddenly at the initial stage, then decreased drastically, after that swayed around and approached to a steady value. The time to steady state did not depend on ε and ReT. The effect of particle interactions was to hinder the average sedimentation velocity, and hindrance was directly proportional to ε. The orientation distributions of pollutant particles depended on ReT and ε, especially on the former. Both the standard deviations of vertical and horizontal velocity, the former was larger than the latter, were nearly independent on ε and ReT.

Finite Element Analysis of Elastic-Plastic Contact Mechanic Considering the Effect of Contact Geometry and Material Properties

Each surface of roughness has different shape of asperity which is modeled with various shapes of analytical models. In this paper, the differences among various models of shape of asperity investigate using the Finite Element Method (FEM) and various analytical models. The contact stresses in rough surfaces are calculated analytically using various asperity shape models. Finite element analysis is also carried out assuming three types of material properties namely, the linear, the elastic-perfect plastic and the elastic-nonlinear hardening. The analytical results are compared with the results obtained by the finite element method. The results illustrate for using a deterministic approach which the numerical models are suitable. In hertz model, the result of force is very big in interface of causing deformation plastic, while Model Zhao has almost same result with FEM nonlinear property model. It is observed that the results obtained from Zhao’s model are generally in a better agreement with the results obtained from various finite element models especially in elastic-plastic and plastic zones, hence it may be concluded that Zhao’s model can be used for analyzing the rough surfaces in contact mechanics.

Static Radical Stiffness and Contact Behavior of Nonpneumatic Mechanical Elastic Wheel

Conventional pneumatic tires exhibit disadvantages such as puncture,blowout at high speed,pressure maintenance,etc.Owing to these structural inevitable weaknesses,non?pneumatic tires have been developed and are investigated.A non?pneumatic mechanical elastic wheel(NPMEW)is introduced and investigated as a function of static radical stiffness characteristics and contact behavior.A bench test method is utilized to improve the riding comfort and the traction traffic ability of NPMEW based on tire characteristics test rig,and the static radical stiffness characteristics and the contact behavior of NPMEW are compared with that of an insert supporting run?flat tire(ISRFT).The vertical force?deformation curves and deformed shapes and contact areas of the NPMEW and ISRFT are obtained using a set of vertical loads.The contact behavior is evaluated using extracted geometrical and mechanical feature parameters of the two tires.The results indicate that the NPMEW appears to exhibit considerably high radical stiffness,and the numerical value is dependent on the mechanical characteristic of the flexible tire body and hinge units.NPMEW demonstrates more uniform contact pressure than ISRFT within a certain loading range,and it can efficiently mitigate the problem of stress concentration in ISRFT shoulder under heavy load and enhance the wear resistance and ground grip performances.