[ Objective] To investigate the combined immunization of porcine circovirus 2 (PCV2) inactivated vaccine with PoIL-2,4. [ Methods] A total of 60 crossbred piglets were randomly divided into three groups, including t...[ Objective] To investigate the combined immunization of porcine circovirus 2 (PCV2) inactivated vaccine with PoIL-2,4. [ Methods] A total of 60 crossbred piglets were randomly divided into three groups, including the test group ( inoculation of 0.5 dose PCV2 inactivated vaccine with 0. 1 mL PoIL-2,4 at 14 and 28 day-old), the positive control group (inoculation of 0.5 dose PCV2 inactivated vaccine) and the blank control group. [ Results ] The immune organ index, the lymphocyte transformation rates under different ages and the number of leukocytes and lymphocytes in peripheral blood increased significantly in test group, compared with control group. Moreover, the antibody and neutralizing antibody were also significantly higher in test group than that in control group. The clinical symptoms and pathological changes were not found, and the PC72 was not detected in serum and tissue after challenge test in test group, which indicated that the combined immunization of PCV2 inactivated vaccine with PoIL-2,4 significantly improved the lymphocyte transformation rate, effectively prevented the replication of PCV2 in organism, and enhanced the growth performance of piglets.展开更多
目的研究携带白细胞介素-2(interleukin-2,IL⁃2)/肝细胞生长因子4-kringle拮抗剂(4-kringle antagonist of hepatocyte growth factor,NK4)双基因的减毒沙门菌(Ty21a-pIRES-IL-2-NK4,命名为TPIN)对小鼠体液及细胞免疫功能的影响。方法...目的研究携带白细胞介素-2(interleukin-2,IL⁃2)/肝细胞生长因子4-kringle拮抗剂(4-kringle antagonist of hepatocyte growth factor,NK4)双基因的减毒沙门菌(Ty21a-pIRES-IL-2-NK4,命名为TPIN)对小鼠体液及细胞免疫功能的影响。方法将18只BALB/c小鼠(雌雄各半)随机分为对照(胃管饲服1.5 mL 10%NaHCO3)、Ty21a(胃管饲服0.1 mL Ty21a)、TPIN(胃管饲服0.1 mL TPIN)组,6只/组。初次免疫7 d后加强免疫,共2次,给药第21天,经眼球采血分离血清,ELISA法检测血清IgG抗体水平;无菌分离小鼠胸腺和脾脏,体外分别用Ty21a、TPIN刺激培养的小鼠脾细胞,72 h后,CCK-8法检测小鼠脾细胞增殖能力,ELISA法检测小鼠脾细胞细胞因子表达水平;称重小鼠脾脏和胸腺,计算脾和胸腺指数,并进行脾和胸腺组织HE染色。结果与对照和Ty21a组相比,TPIN组小鼠血清IgG水平(F=111.74,P<0.01)以及脾细胞上清IFNγ、IL-4和IL-10含量均明显升高(F分别为38.21、11.37、26.92,P均<0.05),脾指数和胸腺指数明显升高(F分别为10.419、5.859,P均<0.05)。Ty21a、TPIN组小鼠胸腺组织皮质增宽,淋巴细胞增多,有轻度炎性反应;脾脏组织白髓增多,淋巴细胞增多伴随中性粒细胞浸润。结论TPIN具有良好的免疫保护作用,能明显刺激机体产生体液免疫和细胞免疫,从而对肿瘤可能具有较好的治疗作用。展开更多
基金Supported by the Science and Technology Project of Zhejiang Province(2011C22093)
文摘[ Objective] To investigate the combined immunization of porcine circovirus 2 (PCV2) inactivated vaccine with PoIL-2,4. [ Methods] A total of 60 crossbred piglets were randomly divided into three groups, including the test group ( inoculation of 0.5 dose PCV2 inactivated vaccine with 0. 1 mL PoIL-2,4 at 14 and 28 day-old), the positive control group (inoculation of 0.5 dose PCV2 inactivated vaccine) and the blank control group. [ Results ] The immune organ index, the lymphocyte transformation rates under different ages and the number of leukocytes and lymphocytes in peripheral blood increased significantly in test group, compared with control group. Moreover, the antibody and neutralizing antibody were also significantly higher in test group than that in control group. The clinical symptoms and pathological changes were not found, and the PC72 was not detected in serum and tissue after challenge test in test group, which indicated that the combined immunization of PCV2 inactivated vaccine with PoIL-2,4 significantly improved the lymphocyte transformation rate, effectively prevented the replication of PCV2 in organism, and enhanced the growth performance of piglets.
文摘目的研究携带白细胞介素-2(interleukin-2,IL⁃2)/肝细胞生长因子4-kringle拮抗剂(4-kringle antagonist of hepatocyte growth factor,NK4)双基因的减毒沙门菌(Ty21a-pIRES-IL-2-NK4,命名为TPIN)对小鼠体液及细胞免疫功能的影响。方法将18只BALB/c小鼠(雌雄各半)随机分为对照(胃管饲服1.5 mL 10%NaHCO3)、Ty21a(胃管饲服0.1 mL Ty21a)、TPIN(胃管饲服0.1 mL TPIN)组,6只/组。初次免疫7 d后加强免疫,共2次,给药第21天,经眼球采血分离血清,ELISA法检测血清IgG抗体水平;无菌分离小鼠胸腺和脾脏,体外分别用Ty21a、TPIN刺激培养的小鼠脾细胞,72 h后,CCK-8法检测小鼠脾细胞增殖能力,ELISA法检测小鼠脾细胞细胞因子表达水平;称重小鼠脾脏和胸腺,计算脾和胸腺指数,并进行脾和胸腺组织HE染色。结果与对照和Ty21a组相比,TPIN组小鼠血清IgG水平(F=111.74,P<0.01)以及脾细胞上清IFNγ、IL-4和IL-10含量均明显升高(F分别为38.21、11.37、26.92,P均<0.05),脾指数和胸腺指数明显升高(F分别为10.419、5.859,P均<0.05)。Ty21a、TPIN组小鼠胸腺组织皮质增宽,淋巴细胞增多,有轻度炎性反应;脾脏组织白髓增多,淋巴细胞增多伴随中性粒细胞浸润。结论TPIN具有良好的免疫保护作用,能明显刺激机体产生体液免疫和细胞免疫,从而对肿瘤可能具有较好的治疗作用。