Facile Synthesis and Adsorption Properties of Phosphonated Cellulose Beads for Selective Removal of Low-density Lipoprotein

A novel low-density lipoprotein adsorbent was prepared simply by directly phosphonating porous cellulose beads. Tests in vitro demonstrated that this adsorbent showed quite good adsorption performance for selective removal of low-density lipoprotein from human plasma. The effects of preparation conditions on the lipoprotein sorption properties of the resulted adsorbent were investigated. The adsorption dynamics was also examined.

Protein adsorption in two-dimensional electrochromatography packed with superporous and microporous cellulose beads

Anion-exchange superporous cellulose(DEAE-SC)and microporous cellulose(DEAE-MC)adsorbents were packed in an electrochromatographic column,and the effect of external electric field(eEF)on the dynamic adsorption was investigated.The column was designed to provide longitudinal,transverse or 2-dimensional(2D)eEF.It was found that the electro-kinetic effect caused by the introduction of an electric field played an important role in the dynamic adsorption of bovine serum albumin to the adsorbents.The dynamic binding capacity(DBC)in the presence of 2D eEF was higher than in the presence of a one-dimensional eEF.The effect of flow velocity on the DBC of the two adsorbents was also demonstrated.It was found that the effect of electric field on the DEAE-MC column was more remarkable than that on the DEAE-SC column at the same flow rate,whereas the DEAE-SC column showed higher DBC and adsorption efficiency(AE)than the DEAE-MC column.With increasing flow rate,the DEAE-SC column could still offer high DBC and AE in the presence of the 2D eEF.For example,a DBC of 21.4mg/mL and an AE of 57.7%were obtained even at a flow rate as high as 900cm/h.The results indicate that the 2D electrochromatography packed with the superporous cellulose adsorbent is promising for high-speed protein chromatography.

Development of Porous Spherical Cellulose Bead Production from Corn Cob as an Exfoliating Agent for Cosmetic Industries

The aim of this research was to develop the porous spherical cellulose production from corn cob as an exfoliating agent for cosmetic industries. In process development of the porous spherical cellulose production from corn cob, it was found that alkaline extraction with 10% sodiumhydroxide gave 85.86% purity of cellulose and the crystallinity index of the pulp of 76.08%. Then, the obtained pulp was extracted with acid sodium chlorite. It was found that the acid sodium chlorite extraction increased the cellulose from 85.86% to 91.86%. The obtained pulp was hydrolyse dwithdilute hydrochloric solution. The result shows that the crystallinity increased from 71.13% to 86.31% with smaller crystallite size compared to that obtained acid sodium chlorite extraction. The hydrolysis pulp was used to prepare porous spherical cellulose which has homogeneous and rough surface. The porosity and pore volume of the porous spherical cellulose increased when the amount of calcium carbonate as porogen was increased. The porous spherical cellulose was prepared from the optimized conditions and the physical and chemical properties were analysed and compared with standard commercial beads （Sephadex G-25）. It was found that the porous spherical cellulose had particle size and pH value similar to the standard value. Hazardous substances such as arsenic, lead and mercury were not found in the obtained porous spherical cellulose. The consumers test shows that 87~/＇0 of consumers accepted the product and the overall complacency was great.

ADSORPTION AND RELEASING PROPERTIES OF BEAD CELLULOSE

The adsorption of some dyes on samples of bead cellulose obtained in the Unit of Research-Production 'Cuba 9' was studied. Methylene blue, alizarin red and congo red fitted the adsorption isotherm of Langmuir. Adsorption kinetics at pH = 6 was linear with the square root of time indicating the diffusion is the controlling step. At pH = 12 a non-Fickian trend was observed and adsorption was higher for the first two dyes. Experiments carried out to release the methylene blue occluded in the cellulose beads gave a kinetic behavior of zero order. The study of cytochrome C adsorption was included to test a proteinic material. Crosslinking of bead cellulose was performed with epichlorohydrin decreasing its adsorption capacity in acidic or alkaline solution.