Expression and Purification of Zinc Finger Domain and Central Domain of MDMD2

[ Objective] This study aimed to construct the His-tagged prokaryotic expression vectors harboring zinc finger domain （ZF） and central domain （ acidic domain ＆ zinc finger domain, CT） of MDM2, respectively, and preliminarily identified biologic activity of the purified fusion proteins. [ Method ] ZF and CT cod- ing regions were amplified from MDM2 cDNA library by PCR and separately inserted into prokaryotie expression vector pET28b to construct the recombinant plas- mids. After verification by enzyme digestion, the recombinant plasmids were separately transformed into E. coli DH5c~ competent cells. The expressed recombinant plasmids were purified using Ni-NTA magnetic beads and identified by SDS-PAGE and Western blotting. [ Result] The molecular weight of His-ZF and His-CT fu- sion proteins was 21 and 31 kD, respectively. E Conclusion] Recombinant fusion proteins containing ZF and CT of MDM2 were obtained successfully, which laid the foundation for subsequent protein crystallization and three-dimensional structure analysis.

Mutational analysis of the structure basis for the multimerization function of NifA central domain

In Klebsiella pneumoniae (Kp) NifA central domain, when theconservative amino acid residue Thr-290 in C3 region was replaced by Val, the function of NifA for activating the transcription of nif genes was lost. Thus the conservative Thr-290 residue seems critical for the activation function of NifA central domain. This point mutant of NifA central domain is used to examine the putative multimerization function of NifA central domain by merodiploid experiment. The results showed that the NifA central domain bore the multimerization determinants of NifA protein. A series of truncated mutants of NifA were constructed to determine the structural elements at the central domain critical for multimerization. It demonstrates that amino acid residues 252-453 are involved in the multimerization function of NifA central domain.

Lu-Hf Isotopic Data of the Mbé-Sassa-Mbersi Tonalite (Central Cameroon Domain): Indicator of ca. 1.0 Ga Juvenile Tonian Magmatism in the Region

The Pan-African/Brasiliano orogenic belts are a part of numerous Neoproterozoic orogenic belts that belong to the long-lived orgenic cycle that distancing phase started at the Tonian around 1.0 Ga. The Tonian magmatism fairly documented in the Neoproterozoic belts of Borborema Province (NE-Brazil), seemed so far inexistent in the Central African Orogenic Belt (CAOB) although these two belts were geologically correlated. Through the Lu-Hf geochronological analysis on zircon of tonalite, the present work, coupled with the previous data, suggests the existence of a Tonian age magmatism in the Central Cameroon Domain of the CAOB although the latter is much reworked. The Nguesseck tonalite outcrops in the northern part of the Mbé-Sassa-Mbersi region, in the northern edge of Central Cameroon Domain of the CAOB and in the Tcholliré-Banyo shear zone (TBSZ). The Lu-Hf data obtain on the zircon grains of this tonalite reveal juvenile HfTDM age of ca. 1.0 Ga. This age, combined to the previous geochemical data suggests that the protholites of this tonalite would have extracted from the source during the distancing phase (rifting and oceanization) of the Pan-african/Brasiliano orogeny at the early Neoproterozoic.

Comparison of central macular thickness between two spectral-domain optical coherence tomography in elderly non-mydriatic eyes

AIM: To compare central macular thickness (CMT) measurements obtained by two spectral-domain optical coherence tomography (SD-OCT) exams, and to evaluate measurement reproducibility and agreement between these two exams, and to investigate the relationship between CMT and possible influencing factors such as age, sex, eye (OD/OS), and operators in elderly non-mydriatic eyes. METHODS: Seventy-two normal subjects were included. Every subject underwent CMT measurement twice using one of two SD-OCT (OSE-2000, Moptim, Shenzhen, China & 3-D OCT-1000, Topcon, Tokyo, Japan) instruments respectively where we randomly chose one eye in each patient for the test; these exams were performed by two operators over an hour period with a brief rest between sessions. Comparison of the OSE-2000 and 3-D OCT-1000 CMT measurements was based on paired- t test The mean difference between the CMT measurements was calculated. General linear model analyzed the relationships among eye (OD/OS), operator, sex, and CMT values using age as co-variant. All tests were considered statistically significant at P <0.05. The main outcome measures included CMT. RESULTS: When evaluated with general linear model analysis, CMT measurements were found to have high reproducibility across the two instruments between the two operators for the OSE-2000 single line scan and 3-D OCT-1000 macular scans (P=0.731; P=0.443). There was statistically significant difference in CMT values between the two instruments (P<0.001) and the mean difference was -46.83 mu m at 95% confidence limits (-49.15,-44.51). Age was positively correlated with CMT (beta coefficient =0.516, P=0.001; beta coefficient=0.453, P =0.009) and sex was correlated with CMT from the OSE-2000 (P=0.021) but not with the 3-D OCT-1000 (P=0.056). According to the actual thickness measurements, the CMT of the male was thicker than the female's but there was no statistical difference. There was interaction between sex and eye in OSE-2000 and not in 3-D OCT-1000 (P=0.02; P =0.374). No significant correlation was found between CMT and the influencing factor of eye in both of the instruments (P=0.884; P=0.492). CONCLUSION: Reproducibility of CMT measurement using the two SD-OCTs is excellent in normal eyes according to the operator factor analysis. OSE-2000 has a different posterior retinal boundary of CMT measurement, which results in the CMT value differences, compared with the 3-D OCT-1000. Age is positively correlated with CMT measurement while sex is correlated with CMT in the OSE-2000 but not in the 3-D OCT-1000 and eye (OD/OS) had no correlation with CMT values. Mydriatic drops may not be necessary for CMT measurement using high scan rate SD-OCT in normal eyes in dark room.

FSAC赛车电子电气架构设计

针对传统分布式赛车电子电气架构存在线束杂乱和整车复用性低等问题,提出了FSAC赛车中央网关+域控制器电子电气架构的设计方案。根据FSAC赛事规则进行中央网关和域控制器的硬件设计及软件设计,对此架构进行离线丢包测试和时延测试,并将中央网关和域控制器布置于赛车上进行基础功能测试和功能性验证。结果表明:中央网关以太网在数据间隔25ms以上时未出现丢包,在通信过程中平均延时小于3ms,满足可靠性、实时性要求;赛通信良好未出现数据的误传、漏传、阻塞、系统卡死等现象,中央网关+域控制器在相同动态任务下对资源占用率明显低于传统分布式架构。

汽车电子电气架构的发展及趋势

随着汽车功能的日益增加,传统的电子电气(EE)架构面临很大的挑战,从而迫使整车的电子电气架构不断地演进,从传统的分布式向集中式转变,从面向信号的软件架构向面向服务的软件架构转变,从控制局域网络(CAN)、局域互联网络(LIN)总线向车载以太网通信架构转变。介绍了汽车电子电气架构的现状以及面临的挑战,概述了汽车电子电气架构的演进路线及具体方案技术,结合电子电气架构的演进趋势,对电子电气架构未来的发展方向提出展望。

基于数据-模型混合驱动的电力系统机电暂态快速仿真方法

数据驱动建模方法改变了发电机传统的建模范式,导致传统的机电暂态时域仿真方法无法直接应用于新范式下的电力系统。为此,该文提出一种基于数据-模型混合驱动的机电暂态时域仿真(data and physics driven time domain simulation,DPD-TDS)算法。算法中发电机状态变量与节点注入电流通过数据驱动模型推理计算,并通过网络方程完成节点电压计算,两者交替求解完成仿真。算法提出一种混合驱动范式下的网络代数方程组预处理方法,用以改善仿真的收敛性;算法设计一种中央处理器单元-神经网络处理器单元(central processing unit-neural network processing unit,CPU-NPU)异构计算框架以加速仿真,CPU进行机理模型的微分代数方程求解;NPU作协处理器完成数据驱动模型的前向推理。最后在IEEE-39和Polish-2383系统中将部分或全部发电机替换为数据驱动模型进行验证,仿真结果表明,所提出的仿真算法收敛性好,计算速度快,结果准确。

New Constraints from Pb-Evaporation Zircon Ages of the Méiganga Amphibole-Biotite Gneiss, Central Cameroon, on Proterozoic Crustal Evolution

The amphibole biotite gneiss (ABGn) in the Méiganga area forms part of a meta volcano-sedimentary sequence of the Adamawa Yade domain (AYD), Central African Fold Belt (CAFB). This sequence shows affinity with immature sediments (greywackes, arkoses) with intercalation of mafic lavas or tuffs. New 207Pb/206Pb zircon evaporation ages for two ABGn samples range from 1887 - 2339 Ma and from 675 - 889 Ma, respectively. These ages and evidence from internal zircon structures indicate that igneous rocks of Archean to Paleoproterozoic and of early Neoproterozoic age contributed to the detritus of the sedimentary sequence. The deposition of detritus took place prior to 614 - 619 Ma which represent the syntectonic emplacement of the Méiganga metadiorite. Leucogranites north to the Méiganga area were generated by melting of crust identical to that which provided the source of the ABGn. The metasedimentary sequence investigated in this study is similar to that of the southern part of the AYD and in the Borborema Province, NE Brazil. The tectonic and geochronologic characteristics of the AYD in the Méiganga area support the idea that during the Proterozoic, Central Africa and NE Brazil were part of the same continental landmass.

动态负荷下中央空调能耗全局控制方法

为了进一步降低中央空调能耗,提出一种动态负荷下中央空调能耗全局控制方法。建立时间序列采集空调用户环境和机组设备负载率等参数,利用单指数平滑算法处理时间序列,减少异常冗余数据,完成中央空调动态能耗计算。在获取中央空调动态能耗数值的基础上,构建能耗控制多重约束模型,运用目标函数计算负荷率约束和进出水温约束,根据时域条件求解模型,实现中央空调设备能耗全局控制。实验结果表明,该方法的冷负荷预测绝对误差值在-0.3~0.3 kW范围内,能够准确预测空调的动态负荷。由此证明,该方法可以实现动态负荷下中央空调能耗全局控制,有效减少机组的功耗。

中亚成矿域锂矿床成矿规律及成矿模式

中亚成矿域发育一系列锂矿床,这些矿床主要分布在西伯利亚克拉通南部的造山带中,矿床的形成具有多期性,包括前寒武纪(1.85~1.83Ga)、晚寒武世-早奥陶世(494~483Ma)、早二叠世(294~272Ma)、晚三叠世-早白垩世(220~180Ma)和早白垩世(139~121Ma)等5个成矿期,矿床类型主要为伟晶岩型和花岗岩型。基于成矿构造背景和锂成矿特征的研究,以重要构造线为界,将成矿域划分为2个成矿省和7个成矿带:(1)阿尔泰-东萨彦成矿省,位于成矿域的西部,包括阿尔泰、桑吉伦高地和东萨彦等3个成矿带,主要发育伟晶岩型锂矿床,成矿作用集中在上述前4个成矿期,矿床形成与西伯利亚克拉通和古亚洲洋2个构造体系有关。(2)蒙古-鄂霍茨克成矿省,位于成矿域的东部,包括东外贝加尔成矿带以及Gobi Ugtaal-Baruun Urt和大兴安岭等2个锂远景成矿带,主要发育早白垩世花岗岩型锂矿床,矿床形成主要与蒙古-鄂霍次克构造体系有关。此外,在中国东天山发育少量晚三叠世伟晶岩型锂矿床,构成东天山锂远景成矿带,矿床形成与哈萨克斯坦-准噶尔板块和塔里木板块碰撞有关。中亚成矿域稀有金属花岗岩和大多数稀有金属伟晶岩为花岗质岩浆高分异结晶的产物,其结晶分异的驱动机制主要是热驱动。富含稀有金属的花岗岩和伟晶岩岩浆的形成温度偏低(~650℃),压力变化较大(500~170MPa);锂富集机理为岩浆结晶分异作用和流体不混溶作用。本次研究分别提出了阿尔泰伟晶岩型锂矿床的成矿模式“地壳熔融→深部花岗岩岩基→浅部稀有金属花岗岩岩枝-伟晶岩岩脉”和东外贝加尔花岗岩型锂矿床的成矿模式“地壳熔融→深部花岗岩岩基→浅部花岗岩-稀有金属花岗岩岩株”。

Disorganization of the retinal inner layers as a prognostic factor in eyes with central retinal artery occlusion

AIM: To evaluate baseline foveal disorganisation of retinal inner layers(DRIL) as a prognostic factor in eyes with central retinal artery occlusion(CRAO). METHODS: Twenty-eight CRAO patients who were followed-up between 2010 and 2016 were retrospectively investigated. Demographic characteristics and detailed ophthalmological examination findings of all patients were recorded. Macular thicknesses(MTs) from 5 separate spots and DRIL were measured with spectral-domain optic coherence tomography(SD-OCT). Correlations between DRIL score and logMAR converted visual acuity(VA), change in VA, patient reference time(RT), number of hyperbaric oxygen therapy(HBOT) sessions, MT and MT change were investigated.RESULTS: There was a positive correlation between the DRIL score and the final VA(r=0.787) and a negative correlation with the change in VA(r=-0.763). The RT and MT were closely related to the DRIL score. A negative correlation was found between the number of HBOT sessions and the DRIL score(r=-0.341).CONCLUSION: The DRIL score is a parameter assessed by SD-OCT, which can provide us reliable information regarding the prognosis of visual functions and response to the treatment for CRAO patients at acute phase.

特种车辆跨域集中式中央计算系统的设计

伴随特种车辆功能要求的日渐复杂,高可靠性及稳定性是汽车电子发展的必然趋势。本研究对特种车跨域集中式中央计算系统的设计进行探讨,实现对车身控制、遥控驾驶、中央充放气、悬架调节等多种系统的控制,依托综合硬件资源共享满足特种车的需求,促进特种车智能化操控,推动汽车产业发展。

中心性浆液性脉络膜视网膜病变Henle纤维层频域OCT影像特征

目的观察中心性浆液性脉络膜视网膜病变(CSC)Henle纤维层(HFL)频域光相干断层扫描(SD-OCT)的影像特征。方法采用横断面研究,纳入2017年1月至2021年11月在青岛市第三人民医院眼科确诊的CSC患者35例35眼。其中男23例23眼,女12例12眼;年龄24~60岁,平均(41.14±8.19)岁;病程1 d~6个月。所有患者均应用SD-OCT对黄斑部进行水平单线扫描,分析和总结视网膜下液(SRF)区HFL的影像特征。结果CSC视网膜神经上皮层呈规则圆顶形脱离者26眼,SRF区HFL呈边界1型25眼,占96.15%;呈边界2型7眼,占26.92%;呈完全高反射型17眼,占65.38%。病程≤21 d 21眼,均呈边界1型,部分合并完全高反射型或边界2型;病程>21 d 5眼,均呈完全高反射型,部分合并边界1型或边界2型。其中鼻侧和颞侧脱离区基本对称15眼,6眼为水平位图像,两侧SRF区HFL均呈基本对等的光反射信号;3眼为鼻侧抬高位图像,鼻侧SRF区HFL光反射信号均高于颞侧;6眼为颞侧抬高位图像,颞侧SRF区HFL光反射信号均高于鼻侧。鼻侧和颞侧脱离区不对称11眼,其中3眼为水平位图像,颞侧脱离范围均大于鼻侧,颞侧SRF区HFL光反射信号均高于鼻侧;4眼为鼻侧抬高位图像,3眼颞侧脱离范围大于鼻侧,两侧SRF区HFL呈基本对等的光反射信号,1眼鼻侧脱离范围大于颞侧,鼻侧SRF区HFL光反射信号高于颞侧;4眼为颞侧抬高位图像,3眼鼻侧脱离范围大于颞侧,两侧SRF区HFL呈基本对等的光反射信号,1眼鼻侧脱离范围和高度均大于颞侧,鼻侧SRF区HFL光反射信号高于颞侧。CSC视网膜神经上皮层呈不规则形脱离9眼,SRF区HFL呈边界1型7眼,占77.78%;呈边界2型5眼,占55.56%;呈完全高反射型6眼,占66.67%;呈低反射型4眼,占44.44%;呈模糊型2眼,占22.22%。其中7眼脱离的视网膜神经上皮层走行欠平滑,HFL表现类型随视网膜神经上皮层走向的改变而改变;2眼视网膜神经上皮层脱离低平,抬高侧光反射信号略低于压低侧。结论CSC的SD-OCT图像中SRF区HFL主要呈边界1型和完全高反射型。HFL表现类型受OCT图像倾斜方位,病程,视网膜神经上皮层脱离区的对称性、范围、高度和走向特征的影响而呈规律性改变。

基于新架构的车身域控制器方案研究与设计

随着汽车各项功能和性能要求的提升,汽车分布式电气架构已不能适应市场需求,汽车正在从电子控制单元(ECU)分布式电气架构向域集中式电气架构转变。文章对当前的车身域控制器设计方案进行研究总结,结合当前的市场需求,分析采用域控制器方案的诸多优点。并设计了一种车身域控制器,基于实车功能需求进行了车身域控制器的方案设计,通过台架以及搭载实车进行功能测试,验证了方案的可行性,为该方案的量产奠定基础。

阿姆河盆地别什肯特坳陷及邻区中下侏罗统烃源岩地球化学特征与勘探意义

阿姆河盆地别什肯特坳陷及邻区中下侏罗统发育一套潜在烃源岩,此烃源岩也是一套潜在的含油气系统,但是目前研究仍然薄弱。为此,通过钻井岩心和岩屑有机地球化学分析及盆地模拟方法,研究了别什肯特坳陷及邻区中下侏罗统烃源岩的有机质丰度、类型与成熟度等地球化学特征及烃源岩的形成环境,并明确了油气勘探的方向。研究结果表明:①中下侏罗统海陆过渡相泥岩段烃源岩有机质丰度介于0.50%~3.53%(平均值为1.27%),有机质类型为偏腐殖混合型,有机质热演化程度为介于0.7%~1.1%,为有效烃源岩;②泥岩段上部的浅海相泥质石灰岩段有机质丰度低,为非烃源岩;③泥岩烃源岩可溶有机物以低碳数正构烷烃为主,呈典型单峰,C27甾烷含量高于C29甾烷,三环萜烷和四环萜烷含量高,但是饱/芳比低,孕甾烷含量高,芳烃硫芴含量高,因此,有机质可能来自浅水的宏观底栖藻类,形成于潟湖环境。结论认为,别什肯特坳陷中下侏罗统烃源岩在早白垩世晚期已成熟,以生气为主,生油为辅,现今仍处在生烃高峰阶段,与构造圈闭形成时期配置好,具备较大的成藏潜力;致密砂岩气是该套含油气系统的重要勘探方向。

跨域数据授权运营研究及应用

随着大数据和云计算的发展,数据管理正在打破“数据孤岛”,从面向单域的孤立服务发展到跨域的数据共享与协同服务。基于公共数据授权运营框架,给出了跨域数据授权运营全链路结构,并探讨了跨域数据加工过程中数据隐私和效率的挑战。针对这些挑战,提出了集中式和隐私计算两种数据加工模式,能够在保护数据隐私的同时提高数据加工效率。最后,给出了一个实际场景下跨域数据授权运营平台的应用案例。

粤港澳大湾区建设面临的机遇、挑战及应对策略

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