Optimization of Extraction Process of Clerodendrum philippinum Schauer var. simplex Mlodenke Total Flavonoids(CPTF) by Central Composite Design-Response Surface Methodology

[Objectives] The research aimed to optimize extraction process of Clerodendrum philippinum Schauer var. simplex Mlodenke total flavonoids( CPTF),and provide reference for its development and utilization. [Methods] Based on single-factor test,ethanol concentration,extraction temperature and extraction time were taken as independent variables,and total flavonoids yield was taken as dependent variable. The test was conducted according to central composite design principle. Multivariate linear regression and binomial equation fitting of the result were conducted,and extraction process of CPTF was optimized by using response surface methodology. [Results]The optimal extraction process of CPTF was as below: ethanol concentration 54. 76%,extraction temperature 83. 92℃,extraction time 102. 64 min,solid-liquid ratio 1:20,extraction for twice. [Conclusions] The extraction process of CPTF by central composite design-response surface methodology was simple and feasible,with reliable prediction result,which was suitable for industrial production.

Optimization of Extraction Process of Total Flavonoids from Akebia trifoliata (Thunb.) Koidz. by Central Composite Design-Response Surface Methodology

[Objectives]To optimize extraction process of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,so as to provide references for development and use of Akebia trifoliata( Thunb.) Koidz. [Methods]The extraction rate of total flavonoids of Akebia trifoliata( Thunb.)Koidz. was taken as observation indicator. On the basis of single factor experiment,central composite design( CCD) was used to evaluate the effects of the extraction temperature,extraction time,and ethanol concentration on the extraction process. Multiple linear regression and binomial fitting were used,and response surface methodology( RSM) was used to select the optimum extraction process. [Results] The optimum extraction process conditions for total flavonoids of Akebia trifoliata( Thunb.) Koidz. was extraction temperature: 83. 92 ℃; extraction time:96. 47 min; ethanol concentration: 63. 92%; extraction times: two times; solid to liquid ratio: 1 ∶ 20; extraction rate of total flavonoids:4. 55%. [Conclusions] The central composite design-response surface methodology( CCD-RSM) is simple,convenient,and feasible for extraction of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,and the prediction results are reliable.

星点设计-效应面法优选金荞麦提取工艺

目的优选金荞麦中表儿茶素的提取工艺。方法以微波功率、乙醇浓度、料液比、提取时间为自变量,表儿茶素提取率为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用星点设计-效应面法选取最佳工艺,并进行预测分析。结果最佳工艺条件为微波功率211.89 W,乙醇浓度55.11%,料液比1∶11.43,提取时间6.16 min,金荞麦中表儿茶素提取率的最大估计值为2.041 4%。实验结果与模型预测值相符。结论该方法简便合理,稳定,可预测性较优,可作为金荞麦的提取工艺。

响应面法优化可溶性花生粕蛋白提取工艺

在单因素试验基础上,采用响应面法确定乙醇溶液-酶法提取花生粕蛋白的最佳条件。中心组合设计的独立变量包括乙醇体积分数、液料比、提取时间和pH值,以蛋白提取率为响应值,建立了二阶多项式非线性回归方程和数值模型。结果显示,蛋白提取率二阶模型相关系数为96.9%。响应量主要受pH值、提取时间和液料比的影响。乙醇体积分数为60%、pH值为6.4、提取时间为70 min和液料比为23∶1(m L/g)时可获得最大提取率(63.16%),该最佳提取条件获得了试验结果的充分证实。研究表明,中心组合设计-响应面法可应用于花生粕蛋白提取工艺的优化分析。

Characterization of stereostructure by X-ray and technology of extracting in combination hydrolysis in situ of acankoreanogenin from leaves of Acanthopanax gracilistylus W.W.Smith

To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax gracilistylus W.W.Smith(AGS),the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ(ECHS) was compared with these of traditional methods.The crystal belongs to the monoclinic system,space group P21,with unit cell parameters: a=(8.3652±0.0006) nm,b=(24.721±0.002) nm,and c=(14.5587±0.0011) nm,α=90 °,β=97.850(4) °,γ=90 °,V=2982.51 nm3,Dc = 1.179 mg/m3,and the molecular number(Z) of elementary structures was 2.The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods.Then,central composite design-response surface methodology(CCD-RSM) was adopted for optimizing the extraction rate of ECHS methods.The optimized values of extraction parameters are as follows: for the for extraction process of acid hydrolysis are that extraction time 110.8 min,solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min,solvent-herb ratio 8.7 and the alkali content 8.79%.Finally,the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%.

星点设计-效应面法优选米泔水漂白术炮制工艺

目的优选樟帮特色米泔水漂白术炮制工艺,为米泔水漂白术炮制规范提供科学依据。方法采用星点设计-效应面法进行米泔水漂白术炮制工艺设计,以米泔水用量、漂洗时间、漂洗温度为考察因素;采用HPLC法测定白术内酯Ⅰ、Ⅱ、Ⅲ等成分量,进行加权综合评分计算OD值(归一化值),采用Design Expert进行2次项回归拟合,优选其最佳工艺并进行验证。结果最佳炮制工艺漂洗条件为米泔水用量为9倍、漂洗时间55 h、漂洗温度26℃,在此条件下,白术内酯Ⅰ、Ⅱ、Ⅲ等成分量综合评分OD值为0.960。结论米泔水漂白术在此条件下稳定可行,能为米泔水漂白术饮片生产与质量标准规范提供参考。

响应面法-中心复合试验优选白芍土炒工艺

目的:优选土炒白芍的炮制工艺参数。方法:以芍药苷、芍药内酯苷含量的综合评分为响应值,土炒温度、时间及加土量为自变量,通过响应面法-中心复合试验优选土炒白芍的工艺参数。采用HPLC测定芍药苷、芍药内酯苷含量,流动相乙腈-0.1%磷酸溶液(14∶86),检测波长230 nm。结果:白芍最佳土炒工艺为温度200℃,炒制时间11 min,饮片与灶心土用量比10∶3;芍药苷和芍药内酯苷平均质量分数分别为3.198%,0.800%。结论:白芍土炒后芍药苷和芍药内酯苷含量高于生品。优选的工艺参数稳定可靠,为土炒白芍的质量控制提供参考。