目的:探讨着丝点蛋白B(centromere protein B,CENP-B)抗体阳性的原发性干燥综合征(primary Sj9gren’s syndrome,pSS)患者的临床和免疫学特征。方法:采用横断面研究的研究设计,回顾性纳入2016年1月至2022年8月就诊于北京大学口腔医院并...目的:探讨着丝点蛋白B(centromere protein B,CENP-B)抗体阳性的原发性干燥综合征(primary Sj9gren’s syndrome,pSS)患者的临床和免疫学特征。方法:采用横断面研究的研究设计,回顾性纳入2016年1月至2022年8月就诊于北京大学口腔医院并确诊为pSS的患者,收集分析患者的一般及临床特征、唾液腺造影、唇腺活检病理、血清免疫学及生化检查等资料。将pSS患者分为CENP-B抗体阳性组和阴性组,用SPSS 23.0软件对数据进行描述性分析、单因素及相关性分析,并将CENP-B抗体阳性组分为CENP-B抗体单阳性组及合并其他抗体阳性组进行亚组分析。结果:共纳入288例pSS患者,其中CENP-B抗体阳性组75例(26.0%),阴性组213例(74.0%)。CENP-B抗体阳性组的发病年龄较大,罹患自身免疫性肝病的比例较高,唾液腺肿大的比例较低,SSA/Ro60、Ro52、SSB抗体的阳性率均较低,免疫球蛋白G、类风湿因子的水平较低,免疫球蛋白M的水平较高,总蛋白水平较低,而白蛋白/球蛋白比值、谷丙转氨酶、谷草转氨酶、碱性磷酸酶、γ-谷氨酰转移酶、乳酸脱氢酶的水平较高。亚组分析显示,CENP-B抗体单阳性组的总蛋白、免疫球蛋白A水平低于合并其他抗体阳性组。结论:CENP-B抗体阳性的pSS患者具有独特的临床和免疫学特征,表现为疾病活动性较低,较少发生唾液腺肿大,但易罹患自身免疫性肝病,肝功能生化指标水平偏高,提示CENP-B抗体可能是pSS亚型分类的特异性标志物。展开更多
In order to study the change of the expression of centromere protein B (CENP-B) caused by antisense transfection, proto-eukaryotically expressed fused protein GST-CENP-B (65 ku) was injected into mouse, and a peculiar...In order to study the change of the expression of centromere protein B (CENP-B) caused by antisense transfection, proto-eukaryotically expressed fused protein GST-CENP-B (65 ku) was injected into mouse, and a peculiar anti-CENP-B serum MaCenpB was collected. A strain of transfected HeLa Tet-off cell HaCb, which contains antisense CENP-B expressing vector pBI-EGFP-as-CenpB, was prepared. Northern blot and Western blot were used to analyze the repression of internal CENP-B in transfected cells. According to the growth curve, the proliferation of HeLa (Tet-off) is repressed by antisense CENP-B, and the multiplication time is prolonged for 32.81 h. The analysis of flow cytometry revealed that, compared with HeLa (Tet-off), the G1 cell population of HaCb is increased (AG1=9%) while S fraction is decreased (AS=11%), but the G2/M phase is nearly unchanged (AG2/M=3%). In the meanwhile, the mi-totic index of HaCb declines greatly compared with that of HeLa (Tet-off). Immunofluorescence showed that the展开更多
基金This work was supported by the State Major Basic Research Development Project of China (Grant No. G1999053901)the National Natural Science Foundation of China (Grant Nos. 39430080 and 39970361).
文摘In order to study the change of the expression of centromere protein B (CENP-B) caused by antisense transfection, proto-eukaryotically expressed fused protein GST-CENP-B (65 ku) was injected into mouse, and a peculiar anti-CENP-B serum MaCenpB was collected. A strain of transfected HeLa Tet-off cell HaCb, which contains antisense CENP-B expressing vector pBI-EGFP-as-CenpB, was prepared. Northern blot and Western blot were used to analyze the repression of internal CENP-B in transfected cells. According to the growth curve, the proliferation of HeLa (Tet-off) is repressed by antisense CENP-B, and the multiplication time is prolonged for 32.81 h. The analysis of flow cytometry revealed that, compared with HeLa (Tet-off), the G1 cell population of HaCb is increased (AG1=9%) while S fraction is decreased (AS=11%), but the G2/M phase is nearly unchanged (AG2/M=3%). In the meanwhile, the mi-totic index of HaCb declines greatly compared with that of HeLa (Tet-off). Immunofluorescence showed that the
