猪脑组织中磷脂的综合提取研究

介绍了利用哺乳动物猪脑组织提取神经醇磷脂、卵磷脂和脑磷脂的工艺流程，并对某些关键环节进行了分析讨论，不仅为研究磷脂的生物学效应提供了原料，也为猪脑组织的综合利用开辟了一条可行的途径．

大豆脑磷脂乙酰化改性及其乳化性能测定

以乙酸酐为乙酰化试剂,嘧啶作为弱碱性催化剂,对大豆脑磷脂进行乙酰化改性。通过正交试验优化反应条件:嘧啶用量为30mmol/100g·LEIF,温度为56℃,反应时间为50min,乙酸酐用量为60mmol/100g·LEIF时,脑磷脂乙酰化率(PECD)可达96.4%。对脑磷脂乙酰化之前与之后的乳化性能进行比较表明,乙酰化脑磷脂的乳化稳定性优于高脑磷脂含量的大豆磷脂。

水、甲醇、乙醇对含水脑磷脂液晶结构影响的X射线衍射研究

用小角X射线散射法(SAXS)对含水脑磷脂分别用水、甲醇和乙醇实验所形成的液晶体系结构进行了研究。实验结果证明:在脑磷脂-水体系中,随着水含量增加,脑磷脂和水形成的双分子层液晶体系的层间距变大;在脑磷脂-甲醇体系中,随着甲醇含量增加,它们的层间距变小。在脑磷脂-乙醇体系中,随着乙醇含量的增加,它们的层间距先由小到大,继而又由大变小,然后液晶相逐渐消失,最后变成液态。水和甲醇、乙醇相比,水有使层间距变大的趋势,醇类有使层间距变小的趋势,随着醇中碳链的增长,层间距减小的趋势增大。

从大豆磷脂中分离高纯脑磷脂的研究

研究了用有机溶剂法从大豆磷脂中提取制备高纯度脑磷脂的工艺,大豆磷脂先用丙酮萃洗溶解去除其中的油脂和游离脂肪酸,然后用无水乙醇溶解除去卵磷脂,最后用石油醚萃取沉淀物得高纯度的脑磷脂。对石油醚萃取高纯度脑磷脂的工艺进行了探讨,得到最佳工艺参数为:料液比为1:4(w/V);萃取次数为3次;萃取温度为30℃。在此工艺条件下脑磷脂的得率为91.9%,纯度为93.5%。

抗GM_1抗体和抗脑磷脂抗体与运动神经元疾病

目的通过分析运动神经元疾病MND）患者血清中抗神经节苷脂GM1抗体和抗脑磷脂抗体，探讨MND的免疫机制。方法用ELISA法测定了22例MND患者血清的抗神经节苷脂GM1抗体和抗脑磷脂抗体。结果MND患者抗GM1抗体阳性7例（32％），抗脑磷脂抗体阳性5例（23％），两种抗体均阳性者2例（9％）；在下运动神经元损害为主的MND（U＋L－MND）患者16例中，7例（44％）抗GM1抗体阳性，5例（32％）抗脑磷脂抗体阳性，而上运动神经元损害的MND（U－MND）患者中二种抗体阳性率均为17％（1例），两者比较亦有显著性差异（P＜0．01）。结论MND可能与自身免疫功能失调有关，尤其在L－MND和U＋L－MND中，自身免疫机制可能参与其疾病的发生和发展。

Hypercoagulability in the Context of Pre-Eclampsia: Case-Control Study at the Laquintinie Douala Hospital (Cameroon)

Introduction: Preeclampsia is one of the major causes of maternal and neonatal morbidity and mortality in the world. The complexity of its etio-pathogenesis involves, among other things, hypercoagulability, which alone accounts for about 15% of his deaths. Our objective was to study the parameters of coagulation (prothrombin level, activated cephalin time) in pregnant women with preeclampsia and non-preeclampsia at Laquintinie Hospital in Douala. Methodology: We carried out an analytical cross-sectional case-control study from November 01st, 2018 to May 31st, 2019, in the gynecology and obstetrics department of Laquintinie Hospital in Douala. We included preeclampsia and non-preeclampsia pregnant women with a gestational age greater than or equal to 20 weeks amenorrhea. The variables of interest were age, pregnancy, parity, gestational age, marital status and body mass index, prothrombin level (PL) and activated cephalin time (ACT). Hypercoagulability was defined by the presence of at least one of the following abnormalities: PL > 100%, ACT < 25 seconds. Statistical tests were considered significant for a p-value < 0.05. Results: We recruited 150 pregnant women including 50 preeclampsia matched with 100 non-preeclampsia. The majority age group in both groups was 25 - 30 years (32% versus 37%). We found a high PL in 58% of preeclampsia versus 22% of non-preeclampsia patients (p = <0.001), a low PL in 8% of preeclampsia versus 0% in non-preeclampsia patients (p = 0.004). The ACT was prolonged in 12% of the preeclampsia versus 0% in the non-preeclampsia patients (p = <0.001). Pregnant women with preeclampsia were 4.89 times more likely to develop hypercoagulability than pregnant women without preeclampsia (OR 4.89;CI 2.34 - 10.20;p = <0.001). In linear regression, preeclampsia was significantly associated with PL (correlation coefficient 0.07;p = 0.008). We did not find risk factors associated with hypercoagulability in preeclampsia. Conclusion: Our study confirms the existence of hypercoagulability associated with preeclampsia, in particular in relation to an increase in the level of prothrombin.

从大豆磷脂中分离高纯度脑磷脂的研究

研究了用有机溶剂法从大豆磷脂中提取制备高纯度脑磷脂的工艺,大豆磷脂先用丙酮萃洗溶解去除其中的油脂和游离脂肪酸,然后用无水乙醇溶解除去卵磷脂,最后用石油醚萃取沉淀物得高纯度的脑磷脂。对石油醚萃取高纯度脑磷脂的工艺进行了探讨,得到最佳工艺参数为:料液比为1∶4(w/V);萃取次数为3次;萃取温度为30℃。在此工艺条件下脑磷脂的得率为91.9%,纯度为93.5%。

大通县牦牛脑中磷脂含量的测定

采用紫外分光光度法对10头大通县牦牛进行了脑中磷脂含量的测定,结果测得牦牛脑组织中的磷脂含量为6.79%;同时利用薄层色谱(TLC)对磷脂进行定性分离,测得其主要成分为PC和PE。

子痫前期血清FGF21、ITGβ-1及FOXA1的表达情况及与妊娠结局的相关性

目的探讨子痫前期血清成纤维细胞生长因子-21(FGF21)、整合素β1(ITGβ-1)及叉头盒蛋白A1(FOXA1)的表达情况及与妊娠结局相关性。方法选择2021年3月至2023年2月南京市妇幼保健院接诊的150例子痫前期患者为研究对象,设为病例组,并选择同期在本院孕检的健康孕妇100例作为对照组,比较两组血清FGF21、ITGβ-1及FOXA1水平变化情况,妊娠结局及其之间的相关性分析。结果病例组血清FGF21、ITGβ-1及FOXA1水平较对照组更高,差异均有统计学意义(P<0.05);轻度组患者血清FGF21、ITGβ-1及FOXA1水平均显著低于重度组患者,差异有统计学意义(P<0.05);病例组不良妊娠结局发生率显著高于对照组,新生儿体重及Apgar评分显著低于对照组,差异有统计学意义(P<0.05);相关性分析结果显示,血清FGF21、ITGβ-1及FOXA1和新生儿窒息、早产儿、低出生体重儿之间均呈正相关,和新生儿体重、Apgar评分均呈负相关(P<0.05)。结论在子痫前期患者中血清FGF21、ITGβ-1及FOXA1的表达和不良妊娠结局间关系密切。