Renewable portfolio standard （RPS） schedule requires a specified percentage of renewable electricity development by a specified date, and therefore directly determines the pace of renewable electricity in one area. ...Renewable portfolio standard （RPS） schedule requires a specified percentage of renewable electricity development by a specified date, and therefore directly determines the pace of renewable electricity in one area. The article aims to explore the ways the natural resource endowment and population characteristic affect the adoption of a RPS schedule. We used logistic regression to analyze the data of RPS dynamic schedules of 29 states and Washington, D.C. in the United States. Results show that local renewable potential and the interaction performance between citizen ideology and the league of conversation voters scoreboard are main factors affecting the choice of an RPS schedule, and the effect of the interaction performance between citizen ideology and LCV scoreboard has a higher significance. The finding suggests that strategies for developing renewable energy should be based on resources rather than economical level, and nublic education for environmental protection should be emphasized.展开更多
Since the first intein (Sce VMA) was found in Saccharomydes cerevisiae ATPases gene in 1990, more and more inteins were identified. It is necessary to analyze the new inteins to understand the sequence charateristics ...Since the first intein (Sce VMA) was found in Saccharomydes cerevisiae ATPases gene in 1990, more and more inteins were identified. It is necessary to analyze the new inteins to understand the sequence charateristics of inteins. By searching protein and nucleic acid database systematically, 101 inteins were found, of which 69 inteins contain homing endonuclease motifs. We only analyze the 69 inteins since most inteins are the classic inteins with homing endonuclease motifs. We found that the distribution of these inteins is particular among species and protein. By multiple sequence alignment, some new sequence characteristics were found and the motifs described previously were revised.展开更多
By heterologous expression of a gene from Palaeococcus ferrophilus,a novel recombinant enzyme designated AMPf was obtained and proved to be an amylolytic enzyme with unique catalytic characteristics.The optimal temper...By heterologous expression of a gene from Palaeococcus ferrophilus,a novel recombinant enzyme designated AMPf was obtained and proved to be an amylolytic enzyme with unique catalytic characteristics.The optimal temperature and pH of AMPf were 50℃ and 7.0 respectively.Although sequence analysis and acarbose hydrolysis ability indicated that AMPf belongs to the subfamily GH13_20,interestingly,this enzyme hardly acts on cyclodextrins and pullulan distinguishing it from most enzymes in this subfamily.AMPf hydrolyzes starches to glucose,maltose,maltotriose,and maltotetrose as main products.AMPf mainly liberates glucose from starch with the concentration of 1%(w/v),while it shows malto-oligosaccharide forming ability with higher starch concentration of 4%(w/v).Also,the 4,6-ethylidene-4-nitrophenyl-maltoheptaose hydrolysis ability further indicates the unique combination endo-acting and glucose releasing exo-acting activtity of AMPf.AMPf could utilize maltose and maltotriose to produce malto-oligosaccharides by transglycosylation activity.It was proven AMPf has application protential in malto-oligosaccharides production.展开更多
文摘Renewable portfolio standard （RPS） schedule requires a specified percentage of renewable electricity development by a specified date, and therefore directly determines the pace of renewable electricity in one area. The article aims to explore the ways the natural resource endowment and population characteristic affect the adoption of a RPS schedule. We used logistic regression to analyze the data of RPS dynamic schedules of 29 states and Washington, D.C. in the United States. Results show that local renewable potential and the interaction performance between citizen ideology and the league of conversation voters scoreboard are main factors affecting the choice of an RPS schedule, and the effect of the interaction performance between citizen ideology and LCV scoreboard has a higher significance. The finding suggests that strategies for developing renewable energy should be based on resources rather than economical level, and nublic education for environmental protection should be emphasized.
基金This work was supported by the National Natural Science Foundation of China (Grant Nos. 39770418 and 39570395).
文摘Since the first intein (Sce VMA) was found in Saccharomydes cerevisiae ATPases gene in 1990, more and more inteins were identified. It is necessary to analyze the new inteins to understand the sequence charateristics of inteins. By searching protein and nucleic acid database systematically, 101 inteins were found, of which 69 inteins contain homing endonuclease motifs. We only analyze the 69 inteins since most inteins are the classic inteins with homing endonuclease motifs. We found that the distribution of these inteins is particular among species and protein. By multiple sequence alignment, some new sequence characteristics were found and the motifs described previously were revised.
基金financially supported by National Natural Science Foundation of China(32072268)The Science&Technology Pillar Program of Jiangsu Province(BE2018304)+3 种基金National First class Discipline Program of Food Science and Technology(JUFSTR20180203)National Natural Science Foundation of China(32072164)National Key Research and Development Program of China(2020YFC1606804)National Natural Science Foundation of China(32101990).
文摘By heterologous expression of a gene from Palaeococcus ferrophilus,a novel recombinant enzyme designated AMPf was obtained and proved to be an amylolytic enzyme with unique catalytic characteristics.The optimal temperature and pH of AMPf were 50℃ and 7.0 respectively.Although sequence analysis and acarbose hydrolysis ability indicated that AMPf belongs to the subfamily GH13_20,interestingly,this enzyme hardly acts on cyclodextrins and pullulan distinguishing it from most enzymes in this subfamily.AMPf hydrolyzes starches to glucose,maltose,maltotriose,and maltotetrose as main products.AMPf mainly liberates glucose from starch with the concentration of 1%(w/v),while it shows malto-oligosaccharide forming ability with higher starch concentration of 4%(w/v).Also,the 4,6-ethylidene-4-nitrophenyl-maltoheptaose hydrolysis ability further indicates the unique combination endo-acting and glucose releasing exo-acting activtity of AMPf.AMPf could utilize maltose and maltotriose to produce malto-oligosaccharides by transglycosylation activity.It was proven AMPf has application protential in malto-oligosaccharides production.
