In this work, the interaction between chelerythrine （CHE） and guanosine is studied using UV-vis and fluorescence measurements at various temperatures. The UV-vis spectra show that the increasing guanosine concentrat...In this work, the interaction between chelerythrine （CHE） and guanosine is studied using UV-vis and fluorescence measurements at various temperatures. The UV-vis spectra show that the increasing guanosine concentrations result in the decreasing absorption intensity and red shift of CHE E absorption band （267 nm）. The fluorescence spectra are fitted to linear analysis, yielding a binding constant of 1.04×10^4 L/tool at 298.15 K of CHE with guanosine. Besides, with △rHm^θ = - 8.26 kJ/mol, △rGm^θ = -22.90 kJ/mol, and △rSm^θ = 49.38 J/（mol K） the interaction should be entropy-driven and exterothermic.展开更多
The binding of chelerythrine （CHE） iminium to calf-thymus （CT） DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polariz...The binding of chelerythrine （CHE） iminium to calf-thymus （CT） DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polarization, and the perturbation of the CD spectra of DNA. These changes are characteristic of intercalation. Scatchard plots constructed from fluorescence titration data gave a binding constant of 5.77×10^5 and a binding site size of 3,4 base pairs at 25 ℃. The intercalative interaction is exothermic with a Van＇t Hoff enthalpy of -33.7 kJ/mol. In addition, △G =-32.9 kJ/mol, and △S =-2.9 J/mol/K. The intercalation of CHE into the DNA helix is enthalpy-driven.展开更多
To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV–vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculat...To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV–vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculations. The binding of CHE to cytidine could be characterized by the hypochromic and bathochromic effects in the absorption bands, and the quenching of fluorescence intensity. The spectral data were fit by linear analysis, yielding a binding constant of 2.49 × 10(4) L mol(?1)at 25 °C of CHE and cytidine, and a van’t Hoff enthalpy of ?20.02 kJ/mol for the exothermic interaction in the standard state. In addition, with and , the interactions should be entropy-driven.展开更多
[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Be...[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Besides,the effects of water quality,light source,oxidant,temperature,and pH on stability were investigated.[Results]The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good;the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable,but they are unstable in tap water whether they are protected from light or not;oxidants have a great influence on the stability of sanguinarine and chelerythrine;sanguinarine and chelerythrine are stable at room temperature lower than 54℃;sanguinarine is stable in pH 2.5-7.0,while chelerythrine is stable in pH 2.5-8.0.[Conclusions]The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.展开更多
Nitidine, Chelerythrine and Sanguinarine, all these three alkaloids are benzophenanthridine alkaloids. Nitidine was used as an anti-HIV, anti-malarial and anti-cancer. Chelerythrine had anti-cancer and anti-inflammato...Nitidine, Chelerythrine and Sanguinarine, all these three alkaloids are benzophenanthridine alkaloids. Nitidine was used as an anti-HIV, anti-malarial and anti-cancer. Chelerythrine had anti-cancer and anti-inflammatory activities. Sanguinarine was widely used as an anti-plaquestic and anti-cancer. High performance thin layer chromatography (HPTLC) method was used for simultaneous quantification of Nitidine, Chelerythrine and Sanguinarine in callus extract of Zanthoxylum rhetsa by using Silica gel 60 F254 as stationary phase and ethyl acetate:methanol:water:diethylamine (30:5:2:0.5 v/v) as mobile phase at 280 nm. The linearity concentration range was 5 - 160 μg/band of each alkaloid. The Rf values of Nitidine, Chelerythrine and Sanguinarine were found to be 0.28, 0.49 and 0.73. The limit of detection and limit of quantification were found to be 0.026, 0.088 μg/spot and 0.010 and 0.033 μg/spot, 0.0104 and 0.035 μg/spot respectively for Nitidine, Chelerythrine and Sanguinarine. HPTLC method was developed and validated according to ICH guidelines for simultaneous estimation of Nitidine, Chelerythrine and Sanguinarine and proved to be simple, specific, accurate, robust and rapid.展开更多
目的探讨精神分裂症与抑郁症患者治疗前后血清胆碱酯酶(ChE)水平及PNASS、HAMD量表的变化情况。方法对首发的68例精神分裂症患者(精分组)与50例抑郁症患者(抑郁组)分别于入院第2d、治疗后12周检测其血清ChE水平,并与50例健康体检者(对照...目的探讨精神分裂症与抑郁症患者治疗前后血清胆碱酯酶(ChE)水平及PNASS、HAMD量表的变化情况。方法对首发的68例精神分裂症患者(精分组)与50例抑郁症患者(抑郁组)分别于入院第2d、治疗后12周检测其血清ChE水平,并与50例健康体检者(对照组)进行对照研究。对精分组采用阳性和阴性症状量表(PANSS)、抑郁组采用汉密顿抑郁量表(Hamilton Depression Scale,HAMD)评定病情。结果治疗前精分组、抑郁组、对照组男患者和女患者平均血清ChE水平分别为(13658.12±5236.13 vs 12363.23±4526.32)、(4522.03±1263.52 vs 3802.65±1203.45)、(5698.56±2013.24 vs 4563.56±2033.12)U/L,精分组与抑郁组男、女患者间血清ChE水平比较差异显著(P<0.05),精分组男、女患者血清ChE水平高于对照组,抑郁组男、女患者血清ChE水平低于对照组,差异显著(均P<0.05)。治疗后精分组男、女患者血清ChE水平为(6596.36±2635.12 vs 5365.22±2256.33)U/L,低于治疗前;抑郁组男、女患者血清ChE水平为(5463.12±1265.98 vs 4698.42±2013.65)U/L,高于治疗前,差异显著(P<0.05)。精分组治疗后PANSS量表阳性症状评分、阴性症状评分、精神病理评分及总分较治疗前减少,差异显著(P<0.05);抑郁组治疗后HAMD量表评分低于治疗前,差异显著(P<0.05)。结论精神分裂症患者血清ChE水平显著升高,抑郁症患者显著降低,经治疗后两者血清ChE水平均向好发展。展开更多
基金This project is financed by Jiangxi Boyuan Industry Co.,Ltd.(Jiangxi province,China)
文摘In this work, the interaction between chelerythrine （CHE） and guanosine is studied using UV-vis and fluorescence measurements at various temperatures. The UV-vis spectra show that the increasing guanosine concentrations result in the decreasing absorption intensity and red shift of CHE E absorption band （267 nm）. The fluorescence spectra are fitted to linear analysis, yielding a binding constant of 1.04×10^4 L/tool at 298.15 K of CHE with guanosine. Besides, with △rHm^θ = - 8.26 kJ/mol, △rGm^θ = -22.90 kJ/mol, and △rSm^θ = 49.38 J/（mol K） the interaction should be entropy-driven and exterothermic.
文摘The binding of chelerythrine （CHE） iminium to calf-thymus （CT） DNA is characterized by hypochromism and bathochromism in the absorption band, enhancement of fluorescence intensity, increase in fluorescence polarization, and the perturbation of the CD spectra of DNA. These changes are characteristic of intercalation. Scatchard plots constructed from fluorescence titration data gave a binding constant of 5.77×10^5 and a binding site size of 3,4 base pairs at 25 ℃. The intercalative interaction is exothermic with a Van＇t Hoff enthalpy of -33.7 kJ/mol. In addition, △G =-32.9 kJ/mol, and △S =-2.9 J/mol/K. The intercalation of CHE into the DNA helix is enthalpy-driven.
文摘To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV–vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculations. The binding of CHE to cytidine could be characterized by the hypochromic and bathochromic effects in the absorption bands, and the quenching of fluorescence intensity. The spectral data were fit by linear analysis, yielding a binding constant of 2.49 × 10(4) L mol(?1)at 25 °C of CHE and cytidine, and a van’t Hoff enthalpy of ?20.02 kJ/mol for the exothermic interaction in the standard state. In addition, with and , the interactions should be entropy-driven.
基金Supported by Post-doctoral Foundation Project of Heilongjiang Province(LBHZ16094)Special Research Fund Project for Scientific and Technological Innovation Talents in Harbin City(2017RAQXJ115)Doctoral Starting up Project of Harbin University of Commerce(2019DS114).
文摘[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and chelerythrine in seven solvents were measured by HPLC.Besides,the effects of water quality,light source,oxidant,temperature,and pH on stability were investigated.[Results]The solubility and stability of sanguinarine and chelerythrine in methanol and ethanol are good;the stability of sanguinarine and chelerythrine in distilled water and rainwater is not affected by light and is very stable,but they are unstable in tap water whether they are protected from light or not;oxidants have a great influence on the stability of sanguinarine and chelerythrine;sanguinarine and chelerythrine are stable at room temperature lower than 54℃;sanguinarine is stable in pH 2.5-7.0,while chelerythrine is stable in pH 2.5-8.0.[Conclusions]The good stability of sanguinarine and chelerythrine under specific conditions shows that they have broad development prospects and value.
文摘Nitidine, Chelerythrine and Sanguinarine, all these three alkaloids are benzophenanthridine alkaloids. Nitidine was used as an anti-HIV, anti-malarial and anti-cancer. Chelerythrine had anti-cancer and anti-inflammatory activities. Sanguinarine was widely used as an anti-plaquestic and anti-cancer. High performance thin layer chromatography (HPTLC) method was used for simultaneous quantification of Nitidine, Chelerythrine and Sanguinarine in callus extract of Zanthoxylum rhetsa by using Silica gel 60 F254 as stationary phase and ethyl acetate:methanol:water:diethylamine (30:5:2:0.5 v/v) as mobile phase at 280 nm. The linearity concentration range was 5 - 160 μg/band of each alkaloid. The Rf values of Nitidine, Chelerythrine and Sanguinarine were found to be 0.28, 0.49 and 0.73. The limit of detection and limit of quantification were found to be 0.026, 0.088 μg/spot and 0.010 and 0.033 μg/spot, 0.0104 and 0.035 μg/spot respectively for Nitidine, Chelerythrine and Sanguinarine. HPTLC method was developed and validated according to ICH guidelines for simultaneous estimation of Nitidine, Chelerythrine and Sanguinarine and proved to be simple, specific, accurate, robust and rapid.
文摘目的探讨精神分裂症与抑郁症患者治疗前后血清胆碱酯酶(ChE)水平及PNASS、HAMD量表的变化情况。方法对首发的68例精神分裂症患者(精分组)与50例抑郁症患者(抑郁组)分别于入院第2d、治疗后12周检测其血清ChE水平,并与50例健康体检者(对照组)进行对照研究。对精分组采用阳性和阴性症状量表(PANSS)、抑郁组采用汉密顿抑郁量表(Hamilton Depression Scale,HAMD)评定病情。结果治疗前精分组、抑郁组、对照组男患者和女患者平均血清ChE水平分别为(13658.12±5236.13 vs 12363.23±4526.32)、(4522.03±1263.52 vs 3802.65±1203.45)、(5698.56±2013.24 vs 4563.56±2033.12)U/L,精分组与抑郁组男、女患者间血清ChE水平比较差异显著(P<0.05),精分组男、女患者血清ChE水平高于对照组,抑郁组男、女患者血清ChE水平低于对照组,差异显著(均P<0.05)。治疗后精分组男、女患者血清ChE水平为(6596.36±2635.12 vs 5365.22±2256.33)U/L,低于治疗前;抑郁组男、女患者血清ChE水平为(5463.12±1265.98 vs 4698.42±2013.65)U/L,高于治疗前,差异显著(P<0.05)。精分组治疗后PANSS量表阳性症状评分、阴性症状评分、精神病理评分及总分较治疗前减少,差异显著(P<0.05);抑郁组治疗后HAMD量表评分低于治疗前,差异显著(P<0.05)。结论精神分裂症患者血清ChE水平显著升高,抑郁症患者显著降低,经治疗后两者血清ChE水平均向好发展。
