Effects of dietary Clostridium butyricum and rumen protected fat on meat quality,oxidative stability,and chemical composition of finishing goats

Background Clostridium butyricum(CB)is a probiotic that can regulate intestinal microbial composition and improve meat quality.Rumen protected fat(RPF)has been shown to increase the dietary energy density and provide essential fatty acids.However,it is still unknown whether dietary supplementation with CB and RPF exerts beneficial effects on growth performance and nutritional value of goat meat.This study aimed to investigate the effects of dietary CB and RPF supplementation on growth performance,meat quality,oxidative stability,and meat nutritional value of finishing goats.Thirty-two goats(initial body weight,20.5±0.82 kg)were used in a completely randomized block design with a 2 RPF supplementation(0 vs.30 g/d)×2 CB supplementation(0 vs.1.0 g/d)factorial treatment arrangement.The experiment included a 14-d adaptation and 70-d data and sample collection period.The goats were fed a diet consisted of 400 g/kg peanut seedling and 600 g/kg corn-based concentrate(dry matter basis).Result Interaction between CB and RPF was rarely observed on the variables measured,except that shear force was reduced(P<0.05)by adding CB or RPF alone or their combination;the increased intramuscular fat(IMF)content with adding RPF was more pronounced(P<0.05)with CB than without CB addition.The pH24h(P=0.009),a*values(P=0.007),total antioxidant capacity(P=0.050),glutathione peroxidase activities(P=0.006),concentrations of 18:3(P<0.001),20:5(P=0.003)and total polyunsaturated fatty acids(P=0.048)were increased,whereas the L*values(P<0.001),shear force(P=0.050)and malondialdehyde content(P=0.044)were decreased by adding CB.Furthermore,CB supplementation increased essential amino acid(P=0.027),flavor amino acid(P=0.010)and total amino acid contents(P=0.024)as well as upregulated the expression of lipoprotein lipase(P=0.034)and peroxisome proliferator-activated receptorγ(PPARγ)(P=0.012),and downregulated the expression of stearoyl-CoA desaturase(SCD)(P=0.034).The RPF supplementation increased dry matter intake(P=0.005),averaged daily gain(trend,P=0.058),hot carcass weight(P=0.046),backfat thickness(P=0.006),concentrations of 16:0(P<0.001)and c9-18:1(P=0.002),and decreased the shear force(P<0.001),isoleucine(P=0.049)and lysine content(P=0.003)of meat.In addition,the expressions of acetyl-CoA carboxylase(P=0.003),fatty acid synthase(P=0.038),SCD(P<0.001)and PPARγ(P=0.022)were upregulated due to RPF supplementation,resulting in higher(P<0.001)content of IMF.Conclusions CB and RPF could be fed to goats for improving the growth performance,carcass traits and meat quality,and promote fat deposition by upregulating the expression of lipogenic genes of Longissimus thoracis muscle.

Effects of Different Exogenous Substances on Seed Germination of Isatis indigotica Under Drought Stress and Chemical Composition of Isatis indigotica leaves

This study was to investigate the effects of three exogenous substances on chemical constituents of Isatis indigotica leavesand their efficacy in alleviating drought stress, and explore the methods of applying exogenous substances to efficient cultivationof Isatis indigotica. Polyethylene glycol (PEG) was used to simulate drought stress to deal with seeds of Isatis indigotica at thegermination stage (concentration: 0, 10%, 15%, and 20%). Simultaneous operation of exogenous growth regulators [microbialinoculum (MI), γ-aminobutyric acid (GABA) and salicylic acid (SA)] and PEG were implemented in seeds of Isatis indigotica.The effects of drought stress and the mitigation of exogenous substances were observed by statistics of seed germination potential,germination rate, hypocotyl length, and radicle length of each treatment. The effects of exogenous substances on the content ofalkaloids, crude protein and free amino acids in the leaves of Isatis indigotica grown in a greenhouse were determined after sprayingexogenous substances on the plants. The differences of germination potential, germination rate, hypocotyl length, and radicle lengthamong 15% PEG stress treatment, 10% PEG stress treatment and the control were significant (P<0.05). According to the predesignedgermination standard, the seeds did not germinate under 20% PEG stress treatment. When the PEG concentration was 15%, the resultsof seed germination potential and germination rate after adding MI were significantly different from those under stress alone (P<0.05).When exposed to 10% PEG stress, the supplementation of GABA led to a notable increase in radicle length of Isatis indigotica seeds,showing significant differences compared to other three treatments. The application of MI and GABA under 15% PEG stress resultedin a significant increase in the radicle and hypocotyl length of Isatis indigotica seeds compared to other two treatments. The contentof the total alkaloids in leaves of Isatis indigotica was significantly increased after spraying GABA. Meanwhile, the contents of crudeprotein and the total free amino acids were kept constant after spraying exogenous substances. Application of MI and GABA couldalleviate drought stress of Isatis indigotica. The content of the total alkaloids in leaves of Isatis indigotica could significantly increaseafter spraying GABA.

Chemical Composition of Pineapple Press Residues (Ananas comosus) and Effect on Growth Performance of Rabbit (Oryctolagus cuniculus)

With a view to improving rabbit production performance, a trial on the chemical composition of pineapple press residue (Ananas comosus) and the effect of its incorporation in the ration on rabbit growth performance (Oryctolagus cuniculus) was carried out at the KUATE Cunicole Farm in Bandjoun, in Western Cameroon. To do this, 36 rabbits of the local breed, aged 53 days with an average weight of 1337 ± 119 g were distributed and randomly assigned to 3 experimental rations corresponding respectively to treatments or batches T0, T1 and T2. The animals in treatment T0 received a ration containing no pineapple press residue, while those in treatments T1 and T2 received a ration containing 20% and 40% pineapple press residue, respectively. These residues were dried and ground for chemical composition analysis. The feed served as well as refusals from the previous day were weighed each morning to assess feed intake. The animals were weighed every 7 days to assess weight performance. At the end of the trial which lasted 7 weeks, the animals were fasted for 24 hours, then sacrificed to evaluate carcass characteristics and the relative weights of some digestive organs. The results of this study showed that pineapple press residues had a high crude fiber content (19.2%) and energy (2500 Kcal/kg DM). Their incorporation had no significant effect on feed intake and feed conversion ratio. The average live weight, weight gain and average daily weight gain of the animals receiving the ration with 20% inclusion of pineapple press residue were comparable to those of the control group and significantly higher than those of animals fed with 40% inclusion of pineapple residue. The highest carcass yields were obtained with rabbits fed 20% pineapple press residue in their ration. The cost of feed for the production of a kilogram live weight of rabbit tends to decrease with the ration incorporated with 20% pineapple press residue. Pineapple press residues constitute a by-product that can be recycled and their incorporation at 20% can increase rabbit growth performance and reduce production costs.

Chromatographic Analysis of Chemical Composition of the Genus Rhododendron Plants Growing on the Mountain of Evota （South Yakutia）

The data on chemical compounds of three Rododendron species （Rh. adamsii Rehd., Rh. aureum Georgi. and Rh. lapponicum subsp, parvifolium （Adams） T. Yamaz.） from the mountain of Evota top in South Yakutia are shown. Extracts of these plants was analyzed by method of planar chromatography in thin layers using different specific detectors to exposure of some groups of biologically active matters. During the researches, it was established that polyphenol compounds of different degree of condensment prevail in rhododendron composition; catechines, flavonoids, coumarins, saponins, essential oils, phenol carboxylic acids and arbutin are present too. Due to detected features, all the researched species of rhododendrons are perspective for practical using in food industry and as adaptogenes for maintenance of people health in the conditions of north.

A Comparison Analysis of Chemical Composition of Aerosols in the Dust and Non-Dust Periods in Beijing

Dust events occurred frequently in Beijing in recent years. In this work, 120 aerosol samples were collected in two typical dust events (21-22 March and 15 May) and a non-dust period in Beijing from March to May 2001. Samples were analyzed for major elemental components by the Proton Induced X-ray Emission (PIXE) method. Results show that the enrichment factors of crustal elements such as Mg, Al, and Ti had little differences between the dust period and the non-dust period in Beijing, while the enrichment factors of other elements that have a relation to anthropogenic emissions were very low during the dust period. The results derived by using multivariate factor analysis from the observation data show that the sources such as soil dust, industry, and fuel combustion were among the major contributors to the particles in Beijing.

The Impact of the South Asia High Bimodality on the Chemical Composition of the Upper Troposphere and Lower Stratosphere

The South Asia High （SAH） is the dominant feature of the circulation in the upper troposphere and lower stratosphere （UTLS） during the boreal summer, and the upper tropospheric anticyclonic circulation extends into the lower stratosphere. The preferred locations of the center of the SAH occur in two different regions, and the center can be located over the Iranian Plateau or over the Tibetan Plateau. This bimodality has an impact on the distribution of chemical constituents in the UTLS region. We analyzed water vapor （H20）, carbon monoxide （CO）, and ozone （03） data derived from the Aura Microwave Limb Sounder （MLS） and total column ozone data from the Ozone Monitoring Instrument （OMI）. For the Iranian Plateau mode of the SAH, the tropospheric tracers exhibited a positive anomaly over the Iranian Plateau and a negative anomaly over the Tibetan Plateau, whereas the stratospheric tracer exhibited a negative and a positive anomaly over the Iranian Plateau and the Tibetan Plateau, respectively. For the Tibetan Plateau mode, however, the distribution of the anomaly was the reverse of that found for the chemical species in the UTLS region. Furthermore, the locations of the extrema within the anomaly seemed to differ across chemical species. The anomaly extrema for H20 occurred in the vicinity of the SAH ridgeline, whereas CO and O3 exhibited a northward shift of 4-8 degrees. These impacts of the variation in the SAH on the chemical constitutes in the UTLS region can be attributed in part to the dynamical structure delineated by the tropopause field and the temperature field at 100 hPa.

Chemical Composition of Essential Oil from Chimonanthus praecox Flower

Objective] To analyze the aroma constituents of essential oils from Chi-monanthus praecox. [Method] Extracted by microwave-assisted hydrodistil ation （MAHD）, aroma constituents of essential oils of Chimonanthus praecox flowers were analyzed by gas chromatography-mass spectrometry （GC-MS）. Normalization method was used to determine the constituents quantitatively. [Result] Total y 27 compounds were identified, accounting for 98.85% of total amounts. The main aroma con-stituents are terpenes, aldehydes, esters, alcohols and hydrocarbons. Main compo-nents with relative percentage over 5% are germacrene D （25.62%）, bornyl acetate （16.71%）, caryophyl ene （10.51%）, cis-α-ocimene （5.18%）, γ-elemene （8.05%）, β-linalool （5.01%）, 1-ethenyl-1-methyl-2,4-bis （1-methylethenyl）-cyclohexane （7.18%） and 2,3,4,4α,5,6-hexahydro-1,4αdimethyl-7-（1-methylethyl）-naphthalene （5.20%）. [Conclu-sion] The study provided a theoretical reference for the development and utilization of Chimonanthus praecox resources.

Crystal Structure and Chemical Composition of Ludwigite from Vranovac Ore Deposit (Boranja Mountain,Serbia)

The crystal structure of ludwigite from Vranovac ore deposit （Boranja Mt., Serbia） was refined using the X-ray powder diffraction （XRPD） Rietveld method in the space group Pbam to a final RB=7.45% and RF=5.26%. It has the unit cell dimensions of： a=9.2515（2） A; b=12.3109（2） A; c=3.03712 （7） A; and V=345.91（1） A3. The calculated distances and angles are mostly in good agreement with the Mg2＋-Fe2＋ substitutions across the M（1） and M（3） sites, as well as with the Fe3＋-AI3＋ replacement in the M（4） site. However, the mean observed M（2）-O distance is considerably shorter than prescribed, due to a slight increase of the Fe3＋ content in the M（2） site. Such replacement was compensated by slight increase of the Fe2＋ content in the M（4） site, resulting in the （Mg1.4sFe2＋o.46Fe3＋o.osMno.o2）2.o1 （Fe3＋o.94Fe2＋0.04Al0.02）1.00B1.00Os composition. The formation temperature was estimated to be about 500- 600C. The influences of the various chemical compositions to the crystallographic parameters, M-O distances, M（3） and M（4） sites shift, distortion parameters and estimated valences, were also studied and compared with other reference samples.

Long-Term Frozen Storage Impact on the Antioxidant Capacity and Chemical Composition of Sardinian Myrtle （Myrtus communis L,） Berries

The effects of frozen storage at -18 ℃ up to 6 months, on the antioxidant properties, chemical composition and other physicochemical properties of myrtle berries （Myrtus comrnunis L.） has been evaluated. Berries showed a good resistance to storage decay, and weight loss remained after six months in the 15% range. Results showed that frozen storage up to 6 months affect individual antocyanins content in different ways. Nevertheless the total anthocyanins content was at the end of storage higher than in fresh fruits. The antioxidant capacity calculated with 2,2-diphenyl-l-picrylhidrazyl （DPPH） and 2,2＇-Azino-bis （3-ethylbenzothiazoline-6-sulfonic acid） （ABTS） increased during storage and the calculated r2 showed a positive correlation with the total anthocyanins content. Gluconic and fumaric acid did not changed, quinic acid decreased while malic and shikimic acid increased. The total organic acid content during storage was higher than in fresh fruits causing a slight decrease in pH. Fructose and glucose increased with the same rate and the ratio fructose/glucose remained unchanged （1.06 ± 0.01）, CIE L^＊a^＊b^＊ coordinates showed a shift of the colour to tawny brown after six months storage.

Study on chemical composition of Viscum coloratum and its HPLC fingerprint in different harvest periods

The purpose of this study was to evaluate the similarity of chemical profile of Viscum loloratum harvested in different months using high performance liquid chromatography(HPLC)fingerprint and multivariate statistical analysis,and to determine the optimal harvest time of Viscum coloratum.8 compounds were isolated from Viscum coloratum by a variety of isolation techniques,3 of which were isolated from the genus Viscum L.for the first time.The fingerprint of Viscum coloratum was established by HPLC,and 16 common peaks were obtained,4 of which were identified by comparison with the reference substances.Results showed that the Viscum coloratum harvested in 6 months could be roughly divided into three groups based on the chemical profiles,and the Viscum coloratum harvested in January had higher levels of the marked compounds.The HPLC fingerprint objectively reveals the characteristics of Viscum coloratum harvested in different months,which can be used to evaluate and control the quality of Viscum coloratum harvested in different months.

Chemical Composition of Summertime PM_(2.5) and Its Relationship to Aerosol Optical Properties in Guangzhou,China

Urban aerosols have a large effect on the deterioration of air quality and the degradation of atmospheric visibility.Characterization of the chemical composition of PM 2.5 and in situ measurements of the optical properties of aerosols were conducted in July 2008 at an urban site in Guangzhou,Southern China.The mean PM 2.5 concentration for the entire period was 53.7±23.2 μg m 3.The mean PM 2.5 concentration (82.7±25.4 μg m 3) on hazy days was roughly two times higher than that on clear days (38.8±8.7 μg m 3).The total water-soluble ion species and the total average carbon accounted for 47.9%±4.3% and 35.2%±4.5%,respectively,of the major components of PM 2.5.The increase of secondary and carbonaceous aerosols,in particular ammonium sulfate,played an important role in the formation of haze pollution.The mean absorption and scattering coefficients and the single scattering albedo over the whole period were 53±20 M m 1,226±111 M m 1,and 0.80±0.04,respectively.PM 2.5 had a high linear correlation with the aerosol extinction coefficient,elemental carbon (EC) was correlated with aerosol absorption,and organic carbon (OC) and SO 4 2 were tightly linked to aerosol scattering.

Effect of Water Depth on Seasonal Variation in the Chemical Composition of Akamoku, Sargassum horneri (Turner) C. Agardh

The brown alga, Akamoku, also known as Sargassum horneri, was harvested at depths of 1 - 2 and 5 m in the oceanic areas of the Chikuzen Sea off the coast of Fukuoka, Japan, and its chemical composition (moisture, protein, lipid, ash, carbohydrate and total dietary fiber content) was investigated. The aim of this study was to elucidate the relationship between the chemical components and the growth of S. horneri as recyclable resource for a sustainable future. S. horneri at an ocean depth of 5 m grew rapidly. The biomass was abundant from January until April. After maturation and gamete release at the middle of April, plant senescence began occurring, and only short plants remained at the sea bottom. On the other hand, S. horneri at an ocean depth of 1 - 2 m grew slowly and could be harvested until early June. The protein, lipid and total dietary fiber content of S. horneri at an ocean depth of 5 m showed apparent gender-based differences.

Chemical Composition of Some Saline Lakes in the Tuva Region(Russia)

1 Introduction Salt lakes are very interesting natural objects which can be found in different places in the world.Russia is not an exception.Climate conditions are various in Russia,but the climate is not very hot.In Russia there are a lot of places where salt lakes are spread.One of them is the Tuva region where lakes with different compositions are

Chemical Composition of Ancient Celadon Material(1127-1279 A.D.) from Zhejiang,China and Its Implication

The microprobe EDXRF equipment was used for analysis of the major and trace elements in glaze layer-transitive layer-body layer of the celadon from the Altar Yao （Kiln） and Laohudong Yao in the Southern Song Dynasty （1127-1279 A.D.）, Zhejiang, China. The K values of the discriminant factor for the celadon wares are larger than 8, which means the celadon of the Altar Yao and Laohudong Yao are different from that of the Longquan Yao. The former two belong to the Guan Yao system （the Chinese imperial kilns）, but the latter to the Min Yao system （the Chinese popular kilns）. The principle component analysis shows their relationship between the Altar and Laohudong wares with provenance postulation. The thickness of the transitive layer in the Altar and Laohudong wares is obviously different, which reveals the microstructure characteristics of the celadon even though both kinds of wares belong to the imperial kiln system.

Studies on the chemical composition of some planktonic copepods in the Taiwan Strait

The chemical composition of marine zooplankton was studied though two seasonal cruises (August 1994and February 1995) in the Taiwan Strait. The carbon(C), nitrogen(N) and hydrogen(H) dry weight contents ofplanktonic copepods were in the range of 36. 24% -45. 63%,8 .50% -11. 37% and 4. 23%-6. 50% repectively.Data comparison among the three southern sea areas of China showed the same subtropical characteristics in the chemical composition of planktonic crustaceans. The seasonal changes of C and N contents and C/N ratio in the TaiwanStrait implied the different nutritional conditions in the open sea from those in the inner gulf.

Chemical Composition of Potato Tubers in Relation to Crop Production System and Environmental Conditions

In the years 2008-2010, a study on the quality of tubers grown in the two crop production systems, i.e., organic and integrated in two locations： Jadwisin--Central and Osiny--South-Eastern of Poland were conducted. The influence of such factors as the crop production system （organic and integrated）, the place of production （soil quality）, variety and years of research on the chemical composition, i.e., tuber dry matter, starch, vitamin C, nitrates and glycoalkaloids were tested. It has been found that the greatest impact on the chemical composition of tubers had the climatic conditions during the growing season and genotype. They affected the contents of all tested components. The place of production affected the contents of dry matter, starch, vitamin C and nitrates. This factor had no effect on the content of glycoalkaloids. The least effect on the chemical tubers composition was in crop production system. It only affected the contents of dry matter, starch and vitamin C. There was no significant effect of this factor on the nitrate and glycoalkaloids content.

Chemical Composition of Artemisia subdigitata Mattf.

[Objectives] The aim was to study the chemical composition of the aboveground part of Artemisia subdigitata Mattf. [Methods]Using the systemic preparative test,the water extract,ethanol extract and petroleum ether extract of the aboveground part of A. subdigitata were subjected to physiochemical identification,and the contained chemical constituents were identified preliminarily. [Results] The preliminary comparisons of preparative test results of the aboveground part of A. subdigitata suggested that the whole plant may contain saponins,tannins,phenols,flavonoids,anthraquinones,coumarins,lactones,alkaloids,sterides or triterpenes,volatile oils and oils. [Conclusions]A. subdigitata contains a variety of chemical constituents. This study provides certain experimental basis for the further research and development and utilization of A. subdigitata.

Chemical Composition of Sphyraena barracuda with Different Area, Size and Season, in Sudanese Red Sea Coast

This study aims to determine the effect of area, size and season on chemical composition of Sphyraena barracuda from Sudanese Red Sea Coast. A total of 180 fish samples were collected from Port Sudan and Sawakin fishing sites during the winter and summer of 2020-2021. The fishes sample ranged between (30 - 50) cm in total length and (250 - 1000) gm in body weight of small size, and ranged between (60 - 90) cm in total length and (1250 - 250) gm in body weight for large size. Fish flesh was subject to gross chemical analysis. Moisture, protein, fat, ash, acidity and pH were determined. The results indicated a significant effect by area, season and size. The different areas had a significant effect on crude protein and ash;higher parameter value is found in Sawakin. Higher chemical parameters were showed in winter than in summer. Also, size had a significant effect in all parameters (except moisture), fat and ash were high in large size. The study has concluded that the large size fishes of S. barracuda with high nutritional value when caught in winter from the southern region of Sawakin.

Distribution and Chemical Composition of Monocarpic Plants of the Apiaceae Family in Tien Shan Mountain

The article presents materials on the distribution and data on the chemical composition of monocarpic plants of the Apiaceae family in the Tien Shan Mountains.

Effects of Different Levels of Fertilization with Hen Droppings on the Production and Chemical Composition of <i>Pennisetum clandestinum</i>(Poaceae)

The use of organic manures, constitute an alternative to that of polluting artificial fertilizers. In this study, the effect of various levels of hen droppings fertilization on the production of biomass, chemical composition and carrying capacity of P. clandestinum were evaluated at the Research and Experimental Farm (REF) and at the Animal Nutrition and Feeding Laboratory of the University of Dschang, between April and September 2016. A completely randomized block design comparing three nitrogen level of fertilization in hen droppings form (0;100 and 200 kg N/ha) on 4 m2 plot of P. clandestinum were used. Each treatment was carried out in four replications for a total of 12 experimental plots. Then a sample of 500 g of forage has been collected in the center of each plot, then dried and used for the evaluation of the biomass and the chemical composition of P. clandestinum, according to the level of fertilization and the cutting frequency. The results showed that the fertilization with the hen droppings positively influenced (p < 0.05) regeneration at the first cutting frequency and the highest value (47.12 cm) was obtained with of 200 kg N/ha rate of fertilization. The biomass of this fodder increased significantly (p < 0.05) with the level of fertilization with hen droppings. Besides, it decreases with the increasing cutting of frequency whatever the level of fertilization. Independently of the fertilization and the cutting frequency, the maximum biomass (6.22 t DM/ha) was obtained with the first cutting at the 200 kg N/ha of hen droppings fertilization. The fertilization influenced in variable way the chemical composition of P. clandestinum. The dry matter and organic matter content decrease with the level of fertilization and the highest values (83.79% and 89.39% respectively for the DM and OM) were obtained on the control plot at the third and the first cutting. Moreover, the ash and crude protein contents increased significantly (p < 0.05) with the cutting frequency of cutting and the level of fertilization applied. The highest ash (19.34% DM) and CP (20.04% DM) contents were obtained with of 200 kg N/ha of hen droppings at the second cutting frequency.