Endocrine disrupting chemicals （EDCs） are increasingly viewed as persistent pollutants, similar to natural hormones in function. This paper describes the expression profiles of 7 genes （DMRT, VTG, GnRHR, FSHR, CYP1...Endocrine disrupting chemicals （EDCs） are increasingly viewed as persistent pollutants, similar to natural hormones in function. This paper describes the expression profiles of 7 genes （DMRT, VTG, GnRHR, FSHR, CYP17A, CYP19A, and CYP19B） involved in sex steroid synthesis and action as well as sexual development in adult male and female Cynoglossus semilaevis, after exposure to different concentrations ofBisphenol A （BPA） and 17[3-estradiol （E2）. Both BPA （1, 10, 50, 125, and 250 mg/kg） and E2 （0.5, 5, and 10 mg/kg） induced changes in target gene expression, although the estrogenic effects orE2 as a model estrogen were stronger. Among the 7 genes, VTG, CYP17A and CYP19 responded strongly to BPA or E2 exposure and can thus serve as reference biomarkers for estrogenic EDCs exposure in marine teleosts. These data will provide a window to establish a hypothalamic-pituitary-gonadal model in C. semilaevis to better understand the effect pathways of EDCs.展开更多
The expression of rat Glutathione S-transferase P (GST-P) gene can be induced by many kinds of chemical inducers. We have studied the mechanisms of the expression of GST-P gene induced by 3 kinds of Chemical inducers ...The expression of rat Glutathione S-transferase P (GST-P) gene can be induced by many kinds of chemical inducers. We have studied the mechanisms of the expression of GST-P gene induced by 3 kinds of Chemical inducers using the induction of the gene expression by chemical inducers, transient transfection, reporter gene assays, electrophoretic mobility shift assays (EMSA) and Northern blot analysis. The results indicate that the effects and mechanisms of chemical inducers on the expression of GS7'-Pgene are different. The interaction of AP-1 or other corresponding transcription factors activated by phorbol ester (TPA) or glycidyl methatylate (GMA) and GPEl enhancer element induces the expression of GST-P gene, while some unknown factors activated by H2O2 interact with GPEII enhancer element.展开更多
基金Supported by the special funds for the Basic R&D Program in the Central Non-profit Research Institutes(No.2060302)
文摘Endocrine disrupting chemicals （EDCs） are increasingly viewed as persistent pollutants, similar to natural hormones in function. This paper describes the expression profiles of 7 genes （DMRT, VTG, GnRHR, FSHR, CYP17A, CYP19A, and CYP19B） involved in sex steroid synthesis and action as well as sexual development in adult male and female Cynoglossus semilaevis, after exposure to different concentrations ofBisphenol A （BPA） and 17[3-estradiol （E2）. Both BPA （1, 10, 50, 125, and 250 mg/kg） and E2 （0.5, 5, and 10 mg/kg） induced changes in target gene expression, although the estrogenic effects orE2 as a model estrogen were stronger. Among the 7 genes, VTG, CYP17A and CYP19 responded strongly to BPA or E2 exposure and can thus serve as reference biomarkers for estrogenic EDCs exposure in marine teleosts. These data will provide a window to establish a hypothalamic-pituitary-gonadal model in C. semilaevis to better understand the effect pathways of EDCs.
文摘The expression of rat Glutathione S-transferase P (GST-P) gene can be induced by many kinds of chemical inducers. We have studied the mechanisms of the expression of GST-P gene induced by 3 kinds of Chemical inducers using the induction of the gene expression by chemical inducers, transient transfection, reporter gene assays, electrophoretic mobility shift assays (EMSA) and Northern blot analysis. The results indicate that the effects and mechanisms of chemical inducers on the expression of GS7'-Pgene are different. The interaction of AP-1 or other corresponding transcription factors activated by phorbol ester (TPA) or glycidyl methatylate (GMA) and GPEl enhancer element induces the expression of GST-P gene, while some unknown factors activated by H2O2 interact with GPEII enhancer element.
