Plants were regenerated from leaves of cherry rootstock Colt by two methods,the frequencies were 48 3% and 21 3%.Leaves were cultured on MS medium supplemented with NAA 1 0mg/L,KT 3 0mg/L and ZT 0 25mg/L.After 5...Plants were regenerated from leaves of cherry rootstock Colt by two methods,the frequencies were 48 3% and 21 3%.Leaves were cultured on MS medium supplemented with NAA 1 0mg/L,KT 3 0mg/L and ZT 0 25mg/L.After 5～7 days leaves dedifferentiated,and formed callus.About 25 days later,callus grew into greenish or pink compact ones,the induction frequency was 100%.The color and structural feature of callus depended on the medium,culture condition and physiology phase of leaves.To induce callus,NAA was the main factor.Leaves rooted in medium only with NAA.In order to inhibit rooting and make callus grow fast,KT and ZT were added to the medium.Leaves cultured without light could quickly form callus during the initial stage of dedifferentiation.Young leaves dedifferentiated more easily than old leaves.After had been cultured about 50 days,callus were transferred to MS medium supplemented with NAA 0 2mg/L,IAA 0 5mg/L,6 BA 0 5mg/L,KT 1 0 mg/L and GA 0 5mg/L,and redifferentiated at a frequency of 21 3%.The callus of Colt leaves were difficult to redifferentiate.GA was important in the redifferentiation of callus.The result showed that callus could redifferentiate in all the mediums with GA,and could not redifferentiate without GA.The ability of redifferentiation of big callus was higher than that of small ones.And the physiology character of callus was also important.High regeneration frequency was obtained from greenish or pink compact callus.The frequency of regeneration of petioles was higher than that of leaves.Leaf petioles' regeneration need high concentration of cytokinin(more than 5 0mg/L).Leaf petioles were cultured on MS medium supplement with 6 BA 6 0mg/L,NAA 1 0mg/L and GA 0 5mg/L,and regenerated at a frequency of 48 3%.展开更多
基金TheprojectwassupportedbytheLocalKeyProjectofChinainLiaoningProvince (No .97-2 1 -0 4)
文摘Plants were regenerated from leaves of cherry rootstock Colt by two methods,the frequencies were 48 3% and 21 3%.Leaves were cultured on MS medium supplemented with NAA 1 0mg/L,KT 3 0mg/L and ZT 0 25mg/L.After 5～7 days leaves dedifferentiated,and formed callus.About 25 days later,callus grew into greenish or pink compact ones,the induction frequency was 100%.The color and structural feature of callus depended on the medium,culture condition and physiology phase of leaves.To induce callus,NAA was the main factor.Leaves rooted in medium only with NAA.In order to inhibit rooting and make callus grow fast,KT and ZT were added to the medium.Leaves cultured without light could quickly form callus during the initial stage of dedifferentiation.Young leaves dedifferentiated more easily than old leaves.After had been cultured about 50 days,callus were transferred to MS medium supplemented with NAA 0 2mg/L,IAA 0 5mg/L,6 BA 0 5mg/L,KT 1 0 mg/L and GA 0 5mg/L,and redifferentiated at a frequency of 21 3%.The callus of Colt leaves were difficult to redifferentiate.GA was important in the redifferentiation of callus.The result showed that callus could redifferentiate in all the mediums with GA,and could not redifferentiate without GA.The ability of redifferentiation of big callus was higher than that of small ones.And the physiology character of callus was also important.High regeneration frequency was obtained from greenish or pink compact callus.The frequency of regeneration of petioles was higher than that of leaves.Leaf petioles' regeneration need high concentration of cytokinin(more than 5 0mg/L).Leaf petioles were cultured on MS medium supplement with 6 BA 6 0mg/L,NAA 1 0mg/L and GA 0 5mg/L,and regenerated at a frequency of 48 3%.
