The bisphosphatase domain of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphos-phosphatase was expressed in high yield Escherichia coli and purified to homogeneity. Single crystals of chicken liver bisphopha...The bisphosphatase domain of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphos-phosphatase was expressed in high yield Escherichia coli and purified to homogeneity. Single crystals of chicken liver bisphophatase domain suitable for X-ray diffraction were obtained by the hanging drop vapor diffusion method. The crystals belong to tetragonal space group P41212 or P43213 with two molecules per asymmetric unit. The determined cell dimensions are: a=b= 10.02 nm, c= 13.98 nm, α=β=г=90°. EHffraction data were collected on Weissenberg camera with synchrotron radiation at 0.32nm resolution.展开更多
Three novel amprolium HCl（AMP）-selective electrodes were investigated with 2-nitrophenyl octylether as a plasticiser in a polymeric matrix of polyvinyl chloride（PVC）. Sensor I was fabricated using potassium tetra...Three novel amprolium HCl（AMP）-selective electrodes were investigated with 2-nitrophenyl octylether as a plasticiser in a polymeric matrix of polyvinyl chloride（PVC）. Sensor I was fabricated using potassium tetrakis（4-chlorophenyl） borate（Tp ClPB） as a cationic exchanger without incorporation of an ionophore.Sensor Ⅱ used 2-hydroxy propyl β-cyclodextrin as an ionophore while sensor Ⅲ used p-tert-butylcalix[8]arene as an ionophore. The three proposed sensors showed Nernestian response slopes of 29.2±0.8,29.3±0.6 and 30.2±0.4 m V/decade over the concentration range from 10–6 to 10–2 mol L_（-1）,respectively. The proposed sensors displayed useful analytical characteristics for the determination of AMP in bulk powder, different pharmaceutical formulations, and chicken liver and in the presence of ethopabate. The proposed method was validated according to ICH guidelines for its linearity, accuracy,precision and robustness.展开更多
To assess the effects ofcreatine pyruvate （Cr-Pyr） on lipid and protein metabolism in broiler chickens, a total of 400 1-day-old male birds （Aconred） were randomly allocated to four groups, with each group replica...To assess the effects ofcreatine pyruvate （Cr-Pyr） on lipid and protein metabolism in broiler chickens, a total of 400 1-day-old male birds （Aconred） were randomly allocated to four groups, with each group replicating four times and each replicate involving 25 birds. The broilers were provided with a commercial diet supplemented with Cr-Pyr at 0, 1, 5, or 10% of the diet, respectively, for a period of 3 wk ad libitum （from 22 to 42 d）. In the present study, body weight （BW） and average daily gain （ADG） of broilers decreased in 10% Cr-Pyr group （P〈0.01）, whereas the relative leg and pectoral muscle weights were significantly higher than they were in the control group （P〈0.05）. 5 or 10% Cr-Pyr of diets decreased the abdominal fat rate （AFR, abdominal fat/live weight） of the broilers. The serum or hepatic triglyceride （TG） concentrations were significantly lower in the 5 and 10% groups （P〈0.01）. In contrast, Cr-Pyr caused a marked increase in the serum nonesterified fatty acid （NEFA）, high-density lipoprotein cholesterol （HDL-C） and creatine kinase （CK） concentrations （P〈0.01）. Supplementation with Cr-Pyr （5 and 10%） in the diet also increased glucagons （GLU）, insulin （INS） or leptin （LEP） contents （P〈0.01）. The expression of hepatic peroxisomal proliferators-activated receptor α （PPAR-α） and carnitine palmitoyl transferase-I （CPT-I）, muscle insulin-like growth factor I （IGF-I） were significantly elevated and myostatin mRNA level was reduced in the 5 and 10% groups （P〈0.05）. It was found that supplementation with 5% Cr-Pyr improves both lipid and protein metabolism by regulating various metabolic parameters of broilers, while not adversely affects growth performance in broiler chickens.展开更多
Background： A leptin-like immunoreactive substance has been found in chicken eggs and has been implicated in serving as a maternal signal to program offspring growth and metabolism. In the present study, we investiga...Background： A leptin-like immunoreactive substance has been found in chicken eggs and has been implicated in serving as a maternal signal to program offspring growth and metabolism. In the present study, we investigated the effects of in ovo leptin administration on hatch weight, serum and hepatic concentrations of metabolites and hormones, as well as on the expression of genes involved in hepatic lipid metabolism and the predicted microRNAs （miRNAs） targeting the affected genes. To this end we injected fertile eggs with either 0.5 μg of recombinant murine leptin or vehicle （PBS） before incubation.Results： Prenatally leptin-exposed chicks showed lower hatch weight, but higher liver weight relative to the body weight, compared to the control group. In ovo leptin treatment increased the hepatic content and serum concentration of leptin in newly hatched chickens. The hepatic contents of triglycerides （TG） and total cholesterol （Tch） were decreased, whereas the serum levels of TG, Tch and apolipoprotein B （ApoB） were increased. The hepatic mRNA expression of sterol regulator element binding protein 1 （SREBP-1c）, SREBP-2, hydroxy-3-methylglutaryl coenzyme A reductase （HMGCR） and cholesterol 7a-hydroxylase 1 （CYP7A1） was significantly up-regulated, as was the protein content of both SREBP-1 c and SREBP-2 in hepatic nuclear extracts of leptin-treated chickens. Moreover, out of 12 miRNAs targeting SREBP-1c and/or HMGCR, five were significantly up-regulated in liver of leptin-treated chicks, including gga-miR-200b and gga-miR-429, which target both SREBP-1c and HMGCR.Conclusions： These results suggest that leptin in ovo decreases hatch weight, and modifies hepatic leptin secretion and lipid metabolism in newly hatched broiler chickens, possibly via microRNA-mediated gene regulation.展开更多
基金Project supported by the Science Foundation of Chinese Academy of Sciences (KZ85-04), the Climbing Project of the State Commission of Science and Technologythe National Natural Science Foundation of China.
文摘The bisphosphatase domain of chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphos-phosphatase was expressed in high yield Escherichia coli and purified to homogeneity. Single crystals of chicken liver bisphophatase domain suitable for X-ray diffraction were obtained by the hanging drop vapor diffusion method. The crystals belong to tetragonal space group P41212 or P43213 with two molecules per asymmetric unit. The determined cell dimensions are: a=b= 10.02 nm, c= 13.98 nm, α=β=г=90°. EHffraction data were collected on Weissenberg camera with synchrotron radiation at 0.32nm resolution.
文摘Three novel amprolium HCl（AMP）-selective electrodes were investigated with 2-nitrophenyl octylether as a plasticiser in a polymeric matrix of polyvinyl chloride（PVC）. Sensor I was fabricated using potassium tetrakis（4-chlorophenyl） borate（Tp ClPB） as a cationic exchanger without incorporation of an ionophore.Sensor Ⅱ used 2-hydroxy propyl β-cyclodextrin as an ionophore while sensor Ⅲ used p-tert-butylcalix[8]arene as an ionophore. The three proposed sensors showed Nernestian response slopes of 29.2±0.8,29.3±0.6 and 30.2±0.4 m V/decade over the concentration range from 10–6 to 10–2 mol L_（-1）,respectively. The proposed sensors displayed useful analytical characteristics for the determination of AMP in bulk powder, different pharmaceutical formulations, and chicken liver and in the presence of ethopabate. The proposed method was validated according to ICH guidelines for its linearity, accuracy,precision and robustness.
基金supported by the the National Natural Science Foundation of China (30600439)
文摘To assess the effects ofcreatine pyruvate （Cr-Pyr） on lipid and protein metabolism in broiler chickens, a total of 400 1-day-old male birds （Aconred） were randomly allocated to four groups, with each group replicating four times and each replicate involving 25 birds. The broilers were provided with a commercial diet supplemented with Cr-Pyr at 0, 1, 5, or 10% of the diet, respectively, for a period of 3 wk ad libitum （from 22 to 42 d）. In the present study, body weight （BW） and average daily gain （ADG） of broilers decreased in 10% Cr-Pyr group （P〈0.01）, whereas the relative leg and pectoral muscle weights were significantly higher than they were in the control group （P〈0.05）. 5 or 10% Cr-Pyr of diets decreased the abdominal fat rate （AFR, abdominal fat/live weight） of the broilers. The serum or hepatic triglyceride （TG） concentrations were significantly lower in the 5 and 10% groups （P〈0.01）. In contrast, Cr-Pyr caused a marked increase in the serum nonesterified fatty acid （NEFA）, high-density lipoprotein cholesterol （HDL-C） and creatine kinase （CK） concentrations （P〈0.01）. Supplementation with Cr-Pyr （5 and 10%） in the diet also increased glucagons （GLU）, insulin （INS） or leptin （LEP） contents （P〈0.01）. The expression of hepatic peroxisomal proliferators-activated receptor α （PPAR-α） and carnitine palmitoyl transferase-I （CPT-I）, muscle insulin-like growth factor I （IGF-I） were significantly elevated and myostatin mRNA level was reduced in the 5 and 10% groups （P〈0.05）. It was found that supplementation with 5% Cr-Pyr improves both lipid and protein metabolism by regulating various metabolic parameters of broilers, while not adversely affects growth performance in broiler chickens.
基金supported by the NSFC-Guangdong Joint Fund (U0931004)the Sino-German Cooperation in Agriculture (Project No. 28/04-05CHN7,2010-2011)+4 种基金the Special Fund for Agro-scientific Research in the Public Interest (201003011)the National "948" project (2011-S11)the Natural Science Foundation of Jiangsu Province (BK2010296)the Doctor Foundation of the Institute of Poultry Science of Jiangsu Province (JQ201001)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Background： A leptin-like immunoreactive substance has been found in chicken eggs and has been implicated in serving as a maternal signal to program offspring growth and metabolism. In the present study, we investigated the effects of in ovo leptin administration on hatch weight, serum and hepatic concentrations of metabolites and hormones, as well as on the expression of genes involved in hepatic lipid metabolism and the predicted microRNAs （miRNAs） targeting the affected genes. To this end we injected fertile eggs with either 0.5 μg of recombinant murine leptin or vehicle （PBS） before incubation.Results： Prenatally leptin-exposed chicks showed lower hatch weight, but higher liver weight relative to the body weight, compared to the control group. In ovo leptin treatment increased the hepatic content and serum concentration of leptin in newly hatched chickens. The hepatic contents of triglycerides （TG） and total cholesterol （Tch） were decreased, whereas the serum levels of TG, Tch and apolipoprotein B （ApoB） were increased. The hepatic mRNA expression of sterol regulator element binding protein 1 （SREBP-1c）, SREBP-2, hydroxy-3-methylglutaryl coenzyme A reductase （HMGCR） and cholesterol 7a-hydroxylase 1 （CYP7A1） was significantly up-regulated, as was the protein content of both SREBP-1 c and SREBP-2 in hepatic nuclear extracts of leptin-treated chickens. Moreover, out of 12 miRNAs targeting SREBP-1c and/or HMGCR, five were significantly up-regulated in liver of leptin-treated chicks, including gga-miR-200b and gga-miR-429, which target both SREBP-1c and HMGCR.Conclusions： These results suggest that leptin in ovo decreases hatch weight, and modifies hepatic leptin secretion and lipid metabolism in newly hatched broiler chickens, possibly via microRNA-mediated gene regulation.
