Creatine Pyruvate Enhances Lipolysis and Protein Synthesis in Broiler Chicken

To assess the effects ofcreatine pyruvate （Cr-Pyr） on lipid and protein metabolism in broiler chickens, a total of 400 1-day-old male birds （Aconred） were randomly allocated to four groups, with each group replicating four times and each replicate involving 25 birds. The broilers were provided with a commercial diet supplemented with Cr-Pyr at 0, 1, 5, or 10% of the diet, respectively, for a period of 3 wk ad libitum （from 22 to 42 d）. In the present study, body weight （BW） and average daily gain （ADG） of broilers decreased in 10% Cr-Pyr group （P〈0.01）, whereas the relative leg and pectoral muscle weights were significantly higher than they were in the control group （P〈0.05）. 5 or 10% Cr-Pyr of diets decreased the abdominal fat rate （AFR, abdominal fat/live weight） of the broilers. The serum or hepatic triglyceride （TG） concentrations were significantly lower in the 5 and 10% groups （P〈0.01）. In contrast, Cr-Pyr caused a marked increase in the serum nonesterified fatty acid （NEFA）, high-density lipoprotein cholesterol （HDL-C） and creatine kinase （CK） concentrations （P〈0.01）. Supplementation with Cr-Pyr （5 and 10%） in the diet also increased glucagons （GLU）, insulin （INS） or leptin （LEP） contents （P〈0.01）. The expression of hepatic peroxisomal proliferators-activated receptor α （PPAR-α） and carnitine palmitoyl transferase-I （CPT-I）, muscle insulin-like growth factor I （IGF-I） were significantly elevated and myostatin mRNA level was reduced in the 5 and 10% groups （P〈0.05）. It was found that supplementation with 5% Cr-Pyr improves both lipid and protein metabolism by regulating various metabolic parameters of broilers, while not adversely affects growth performance in broiler chickens.

Effect of Manganese on DNA-Protein Crosslinks of Testicle in Chicken

In order to explore the effect of manganese on DNA-protein crosslinks （DPC） of testicle in chicken. 500, 800, 1 700 mg·kg^-1 MnC12 were added to forage to establish the model of the sub-chronic manganese poisoning. After 30, 60 and 90 d, testicles were taken out to detect hydroxyl radical inhibiting capacity and DPC content. The results showed that compared with control group, hydroxyl radical inhibiting capacity significantly decreased and DPC content notably increased, and there was a time-dose relationship. It demonstrated that manganese could decrease the inhibitng capacity of hydroxyl radical, increase the content of hydroxyl and DPC, and induce DNA damage.

Comparison of the Serum Proteins and Immune Responses of Velogenic Newcastle Disease Virus Infected Chickens and Ducks

The effect of velogenic Newcastle disease virus (vNDV) on the immune responses and serum proteins was investigated in six-week-old ducks and chickens. Results showed that weight loss was markedly significant (p < 0.05) from days 3 - 21 (PI) in chickens and mild (p < 0.05) on days 3 and 15 PI in ducks. The antibody response obtained showed significant (p < 0.05) increase in infected chickens (IC) than those of the infected ducks (ID). While the total serum protein and serum globulin increased significantly (p < 0.05) in IC on days 7 and 14 PI, they decreased significantly (p < 0.05) in ID only on day 21 PI. The immune responses and serum protein values in this experiment X-ray showed less susceptibility of ducks when compared with the chickens. This may be related to marked anorexia and severe dehydration observed in the latter consequent upon serum concentration. Ducks could be maintaining the endemicity of Newcastle disease (ND) as reservoir host.

Effects of Different Dietary Energy and Protein Levels on Meat Performance and Meat Quality of Jinghai Yellow Chickens

To investigate the effect of different dietary energy and protein levels on meat performance and meat quality of Jinghai yellow chickens, 480 43-day old Jinghai yellow chickens with similar weight were randomly divided into four experimental groups： experimental group 1 （protein 15%, metabolic energy 9.95 MJ/kg）, experimental group 2 （protein 16%, metabolic energy 10.95 MJ/kg）, experimental group 3 （protein 17%, metabolic energy 12.65 MJ/kg） and experimental group 4 （ protein 18%, metabolic energy 13.95 MJ/kg）, respectively. All chickens were slaughtered at 112-day old. The breast and leg muscles of Jinghai yellow chickens were collected, to determine the slaughter performance, conventional meat quality and muscle chemical indicators. The results indicated that dressing-out percentage and eviscerated yield percentage in four experimental groups were above 87.27% and 67.00%, respectively; other slaughter performance indicators exhibited no significant differences among various groups （P 〉 0.05 ） ; breast muscle color of hens in experimental group 4 varied significantly from that in other three groups （ P 〈 0.05 ） ; leg muscle color of hens in experimental group 2 varied extremely significantly from that in other three groups （ P 〈 0.01 ） ; water-holding capacity of breast muscles of hens in experimental group 3 was significantly higher than that in experimental group 4 （P 〈 0.05 ） ; thiamine content of breast muscles of cocks in experimental group 3 was significandy higher than that in experimental group 2 （ P 〈 0.05 ） ; however, other properties exhibited no significant differenees among various groups （P 〉 0.05 ）.

Improving the Dietary Protein Quality by Amino Acid Fortification with a High Inclusion Level of Micro Algae (<i>Spirulina platensis</i>) or Insect Meal (<i>Hermetia illucens</i>) in Meat Type Chicken Diets

The objective of this study was to measure protein quality parameters (PPV, NPU and NPUstd) of chicken diets with complete substitution of soybean meal (SBM) by Spirulina meal (SM) or partly defatted Hermetia meal (HM). N balance experiments were based on the quantitative excreta collection method, divided into starter period (10 - 20 d) and grower period (25 - 35 d). The study utilized 70 all male meat type chicken (Ross 308). Data assessment applied the exponential N utilization model of the “Goettingen approach”. The control diet was based on wheat, corn and SBM. In four experimental diets SBM was completely substituted by SM or HM, but fortified with feed amino acids (AA) both on a basic level of supplementation (Lys and Met added equal to the control diet) and on an extended level (Lys, Met, Thr, Arg, Val, Ile, His added). At a basic level of AA supplementation, complete replacement of SBM by SM or HM in chicken diets depressed dietary protein quality significantly (p 0.05). However, the extended level of AA supplementation improved protein quality parameters of the diets with both of the alternative proteins significantly (p < 0.05), but still generally not on par with the control diet. The observed responses were accentuated when the well-known effect of N intake on protein utilization was eliminated through the standardization of N intake by application of the “Goettingen approach”.

Effects of Supplemental Glutamine and Lysine on Growth Performance of Broiler Chickens

The optimum levels of Lysine and Glutamine needed for growth performance and maintenance of the chicken broilers were evaluated in a randomized 3 × 4 factorial arrangement of dietary treatments. The battery cages measured 99 × 66 × 25 cm that can be sufficient for 5 birds. Day old Chicken broilers totaling 180 were assigned to dietary treatments comprising of 3 concentrations of Lysine (0.85, 1.14, and 1.42) each in combination with 4 concentrations of Glutamine (0, 1, 2, and 3). Each dietary treatment was replicated 3 times and each replication had 5 birds. The birds were given feed and water ad libitum with a 23-hour light regimen for a period of 4 weeks. Then, the experimental birds were evaluated for body weight gain, feed consumption, and feed conversion in order to determine their optimum requirement for dietary Lysine and Glutamine. Based on the findings of this study, the highest performance was observed in birds fed the diet supplemented with 1.42 lysine and 1% glutamine, but the highest improvement in feed conversion was observed in diet contain 1.14 and 1.42 with 1% and 3% glutamine, respectively. Birds fed 1.42 lysine and 1% glutamine had the highest total body weight gain and feed consumption. The lysine requirements in the diet for Chicken are between 1.14 and 1.42 with glutamine level of 1%.

鹰嘴豆分离蛋白对减盐辽宁庄河大骨鸡鸡肉丸凝胶品质的影响

探究添加不同比例(1%、2%、4%)鹰嘴豆分离蛋白(chickpea protein isolate,CPI)对减盐大骨鸡鸡肉丸凝胶品质的影响。测定不同处理组减盐大骨鸡鸡肉丸的质构特性、色泽、水分分布、流变特性和微观结构。结果表明:(1.4%NaCl、4%CPI)处理组的减盐大骨鸡鸡肉丸的硬度、弹性、咀嚼性显著高于其他处理组(P<0.05)。低场核磁共振分析显示,CPI添加量为4%的减盐大骨鸡鸡肉丸具有最高的P21和最低的P22,证明该条件下的减盐大骨鸡鸡肉丸持水性最佳。流变学特性显示,(1.4%NaCl、4%CPI)处理组的减盐大骨鸡鸡肉丸的储能模量最大,弹性最佳。扫描电镜显示,(1.4%NaCl、4%CPI)处理组的减盐大骨鸡鸡肉丸凝胶与其他处理组相比,结构更紧实,连续性更好。综上所述,添加1.4%NaCl、4%CPI有利于提高CPI减盐大骨鸡鸡肉丸的凝胶品质。

鸡胸肉酶解液美拉德反应工艺优化及应用

为推进鸡肉蛋白高值化利用,提升其风味品质,该文以鸡胸肉酶解液为原料制备美拉德反应产物,采用单因素和正交试验确定美拉德反应的最佳工艺条件,进一步将美拉德反应产物与酵母蛋白进行复配,通过调味、调质开发一款风味口感较佳的高蛋白饮品。研究结果显示:美拉德反应的最佳工艺为反应温度95℃、反应时间1.5 h、葡萄糖与鸡胸肉酶解液质量比1∶15。通过单因素试验,确定酵母蛋白添加量为12.5%。对美拉德反应产物与酵母蛋白的混合溶液进行调味、调质,确定最佳配方为柠檬汁添加量2%、薄荷粉添加量0.03%、甘露糖醇添加量0.6%、黄原胶添加量0.06%。鸡胸肉酶解液经美拉德反应与调味后,在口感上得到较大改善。

Relationship Between Combined Genotypes of UCP Gene and Growth Traits in Chickens

The uncoupling protein （UCP） is a member of the mitochondrial membrane transporter family, which plays an important role in energy metabolism. In the present study, the UCP gene was considered as a candidate gene for chicken growth traits, and the association of UCP gene SNPs with growth rate was investigated in the eighth generation of NEAUHLF broiler lines. Two SNPs were found in chicken UCP gene, and the association analysis results showed that both the individual and combination of chicken UCPgene SNPs were significantly associated with body weight of 7 weeks （BW7） and carcass weight （CW） （P〈0.05）, and the combination had much significant effects than the single SNP. This research suggested that the UCP gene could be a candidate gene or linked to a major gene which affected growth traits in chicken

Transcriptome responses to heat stress in hypothalamus of a meat-type chicken

Background： Heat stress has resulted in great losses in poultry production. To address this issue, we systematically analyzed chicken hypothalamus transcriptome responses to thermal stress using a 44 k chicken Agilent microarray, Methods： Hypothalamus samples were collected from a control group reared at 25℃, a heat-stress group treated at 34℃ for 24 h, and a temperature-recovery group reared at 25℃ for 24 h following a heat-stress treatment. We compared the expression profiles between each pair of the three groups using microarray data. Results： A total of 1,967 probe sets were found to be differentially expressed in the three comparisons with P 〈 0.05 and a fold change （FC） higher than 1.5, and the genes were mainly involved in self-regulation and compensation required to maintain homeostasis. Consistent expression results were found for 11 selected genes by quantitative real-time PCR. Thirty-eight interesting differential expression genes were found from GO term annotation and those genes were related to meat quality, growth, and crucial enzymes. Using these genes for genetic network analysis, we obtained three genetic networks. Moreover, the transcripts of heat-shock protein, including Hsp 40 and Hsp 90, were significantly altered in response to thermal stress. Conclusions： This study provides a broader understanding of molecular mechanisms underlying stress response in chickens and discovery of novel genes that are regulated in a specific thermal-stress manner.

Experimental Study on the Antitumor Effect of Chicken Anemia Virus vp3 Gene against Liver Carcinoma In Vivo

In order to testify the antitumor effect, especially its effect against liver carcinoma in vivo, of VP3 protein, one kind of protein coded by chicken anemia virus, recombinants pcDNA-vp3 containing chicken anemia virus vp3 gene, and control vector pcDNA3 were mixed with murine liver carcinoma cell lines H22 respectively. The mixture was injected subcutaneously into Balb/C mice. Some days later, the mice were killed and the solid tumor weighed. The antitumor efficiency was evaluated. The manners of VP3 protein in vivo inducing tumor cell death were identified by using TUNEL assay. All the results suggested that the injection of pcDNA-vp3 and H22 mixture resulted in a significant reduction of tumor growth in mice when compared with the results of control groups. TUNEL assay revealed that VP3 induced apoptosis in vivo. All these indicated that CAV vp3 might be a potential new gene in reducing the growth rate of tumor cells in liver carcinoma or in other kind of solid tumors in vivo.

The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum

Chicken anaemia virus (CAV) causes a viral disease in chickens worldwide and thus has economic importance. The main aim of this study was to develop a rapid, sensitive and specific VP1-CAVI indirect ELISA for the detection of CAV infection. The CAV-VP1, was separately cloned and expressed in recombinant E. coli. The purified recombinant CAV-VP1 protein was then coated as an antigen on an ELISA plates to evaluate its reactivity against chicken sera. The resulting indirect ELISA was then compared with a commercial ELISA. The specificity and sensitivity of the indirect ELISA were measured as 93.3% and 100%, respectively. A t-test produced a t-value of 15.805 for the indirect ELISA and revealed a significant difference between CAV-positive serum and CAV-negative serum (p-value of 0.001). For the second variable (i.e., a commercial ELISA), the t-test yielded a t-value of 5.063, which revealed a significant difference between CAV-positive serum and CAV-negative serum (p-value of 0.015). This intervention produces statistically significant improvements in both variables (p-values < 0.05). The correlation coefficient for the indirect ELISA was r = 0.93. Therefore, this work can be considered as a new achievement in diagnosis for Chicken anaemia virus in chicken flocks.

饲粮代谢能和粗蛋白水平对产蛋后期双莲鸡生产性能、蛋品质和肝脏脂代谢的影响

试验旨在研究饲粮不同代谢能和粗蛋白水平对产蛋后期双莲鸡配套系生产性能、蛋品质、肝脏脂代谢的影响。试验选取240只健康、体重及产蛋率接近的40周龄双莲鸡产蛋母鸡,随机分为6组,每组4个重复,每个重复10只鸡。试验采取2×3双因素设计,代谢能(ME)设定为11.30和10.88 MJ/kg两个水平,粗蛋白(CP)设定为16.2%、15.2%和14.2%三个水平,预试期2周,正试期12周。结果显示:(1)粗蛋白水平对产蛋率、料蛋比、平均蛋重无显著影响(P>0.05),但11.30 MJ/kg代谢能水平可显著降低51~54周龄双莲鸡产蛋率和平均日产蛋量(P<0.05),并显著增加平均蛋重(P<0.05)。(2)饲粮代谢能和粗蛋白互作极显著影响46周龄鸡蛋蛋白高度和哈氏单位(P<0.01),并显著影响50周龄鸡蛋蛋黄颜色(P<0.05);10.88 MJ/kg代谢能水平54周龄鸡蛋哈氏单位、蛋黄比例显著高于11.30 MJ/kg代谢能水平(P<0.05),而46、54周龄鸡蛋蛋黄颜色和46、54周龄鸡蛋蛋壳厚度极显著低于11.30 MJ/kg代谢能水平(P<0.01),50周龄蛋壳厚度显著低于11.30 MJ/kg代谢能水平(P<0.05);16.2%粗蛋白水平46周龄鸡蛋哈氏单位显著高于14.2%粗蛋白水平(P<0.05),而54周龄鸡蛋蛋黄比例极显著低于15.2%和14.2%粗蛋白水平(P<0.01)。(3)10.88 MJ/kg代谢能水平双莲鸡血清、肝脏甘油三酯水平,肝脏相对重量和腹脂相对重量以及肝脏脂肪酸合成相关基因如SREBP-1c、ACACA、FAS、SCD1表达量均显著低于11.30 MJ/kg代谢能水平(P<0.01)。综合分析,本试验条件下双莲鸡配套系蛋鸡产蛋后期(42~54周龄)代谢能和粗蛋白水平分别以10.88 MJ/kg和16.2%为宜。

抗鸡传染性贫血病毒VP2蛋白单克隆抗体的制备及其应用研究

为制备针对鸡传染性贫血病毒(Chicken infectious anemia virus,CIAV)的单克隆抗体,以CIAV的VP2原核表达蛋白作为抗原免疫小鼠,经杂交瘤技术获得3株能稳定分泌抗VP2蛋白特异性抗体的细胞株,进一步经IFA筛选出特异性和灵敏度俱佳的杂交瘤细胞Mab-VP2-4株,制备的小鼠腹水效价达到1∶2000。结果显示,制备的抗VP2蛋白单克隆抗体可与多株CIAV感染的MDCC-MSB1细胞发生特异性反应,而对EDSV、ALV、ILTV、ARV以及空白MDCC-MSB1细胞、CEF细胞等均无交叉反应,特异性良好,且应用于IFA方法时对CIAV感染的最低检出限为5 TCID 50。以该单克隆抗体对市场上10种不同来源的禽活疫苗进行病毒分离结合单克隆抗体IFA检测,同时与《中国兽药典》规定的鸡检查法进行对比检测,结果一致,均未检出CIAV污染。本研究制备了针对CIAV VP2蛋白的特异性单克隆抗体,为该病特异性诊断方法的建立奠定了基础。

发酵茶渣蛋白的提取及其对皖南三黄鸡养分表观代谢率的影响

试验旨在研究发酵茶渣蛋白的提取及其对皖南三黄鸡养分表观代谢率的影响。试验以发酵茶渣为原料,采用碱溶酸沉法提取茶蛋白,以茶蛋白的提取率作为正交试验的评定指标,得出最优提取工艺。选取1日龄皖南三黄鸡120只,随机分成4组,每组3个重复,每个重复10只,对照组饲喂基础饲粮,试验组分别在基础饲粮中添加0.6%、0.8%和1.0%的茶蛋白。预试期7 d,正式试验期28 d。于31日龄时进行代谢试验,分析饲粮干物质(DM)、粗蛋白(CP)、粗脂肪(EE)、钙(Ca)和磷(P)的表观代谢率。结果表明:茶蛋白的最佳提取条件为NaOH浓度0.15 mol/L、提取时间80 min、提取温度75℃、液料比50 mL/g。在此工艺条件下,发酵茶渣中茶蛋白的提取率为51.09%。饲粮添加0.8%、1.0%茶蛋白皖南三黄鸡干物质、粗蛋白、粗脂肪、钙及磷的表观代谢率显著高于对照组(P<0.05)。研究表明,饲粮中添加0.8%~1.0%的茶蛋白,可以提高皖南三黄鸡对饲粮养分的表观代谢率。

解冻方式对速冻鸡肉饼品质的影响

以鸡肉饼为研究对象,探讨不同解冻方式对调理鸡肉饼品质的影响。将新鲜鸡肉饼置于-40℃的冰箱速冻贮藏12 h,然后采用室温解冻、低温解冻、水浸解冻和微波解冻4种方式进行解冻,通过对鸡肉饼的色泽、硫代巴比妥酸反应物(thiobarbituric acid reactive substances,TBARS)值、蒸煮损失率、解冻损失率和总巯基含量等指标进行测定,分析不同解冻方式鸡肉饼的品质变化规律。结果表明:各处理组鸡肉饼pH值均无显著差异(P>0.05);微波解冻组鸡肉饼红度值显著低于室温解冻组、水浸解冻组、低温解冻组(P<0.05);低温解冻组的鸡肉饼解冻损失率、蒸煮损失率、TBARS值显著低于室温解冻和微波解冻组(P>0.05);低温解冻组的鸡肉饼巯基含量均高于各解冻处理组。综上,低温解冻是4种解冻方式中的最佳解冻方式,可以有效保持鸡肉饼的品质。

能量与蛋白质水平对静原鸡生长性能、屠宰性能、血液生化指标及代谢组的影响

【目的】研究不同能量和蛋白质水平对育成期静原鸡生长性能、屠宰性能、血液生化及代谢组的影响,为确定育成期静原鸡适宜日粮能量、蛋白质水平提供依据。【方法】选取7周龄静原鸡公鸡540只,随机分为9组,每组6个重复,每个重复10只鸡。采用2因子×3水平试验设计,因子1为日粮能量水平,设有低、中、高能量组,分别为11.28、11.70、12.12 MJ/kg,因子2为日粮蛋白质水平,设有低、中、高蛋白质组,分别为14.00%、15.50%、17.00%,试验期77 d。【结果】①日粮能量、蛋白质水平及交互作用显著影响末重、平均日增重、平均日采食量和料重比(P<0.05,下同),中能量-中蛋白质组(ME-MP组)末重、平均日增重最高,料重比最低;低能量-高蛋白质组(LE-HP组)平均日采食量最低。②日粮能量水平显著影响胸肌率、腿肌率,中能量组胸肌率、腿肌率最高。日粮能量、蛋白质交互作用对屠体率、半净膛率有显著影响,中能量-中蛋白质组(ME-MP组)屠体率、胸肌率、腿肌率最高。③日粮能量水平显著影响尿素含量,中能量组尿素含量最低。日粮蛋白质水平显著影响血糖,高蛋白质组血糖含量最高。日粮能量、蛋白质交互作用显著影响血清总蛋白、白蛋白及游离脂肪酸,中能量-中蛋白质组(ME-MP组)血清总蛋白含量最高、高能量-高蛋白质组(HE-HP组)血清白蛋白含量最高、中能量-高蛋白质组(ME-HP组)游离脂肪酸含量最高。④通过血液代谢组学分析共筛选出16种显著差异代谢物,主要富集在β-丙氨酸代谢、花生四烯酸新陈代谢通路、苯丙氨酸、酪氨酸和色氨酸生物合成、初级胆汁酸生物合成、缬氨酸、亮氨酸、异亮氨酸降解等通路。【结论】日粮能量、蛋白质水平可显著影响育成期静原鸡公鸡生长性能、屠宰性能,这可能与其通过影响血清生化指标及代谢通路调控有关,能量、蛋白质需要量综合表明中能量-中蛋白质水平适宜于育成期静原鸡公鸡营养需要,其推荐水平分别为11.70 MJ/kg,蛋白质水平为15.50%。

大豆分离蛋白-黄原胶乳液替代脂肪对煎制鸡肉饼理化特性的影响

为开发蛋白质-多糖基复合乳液在低脂肉制品中的应用,优化了以大豆分离蛋白(SPI)、黄原胶(XG)和大豆油为原料的大豆分离蛋白-黄原胶(SPI-XG)乳液的制备工艺,并探究不同替代量的SPI-XG乳液对鸡肉饼理化特性的影响。结果表明:当pH值为9时,最佳的SPI-XG乳液配方为SPI 6%、XG 0.2%、油液比3∶1。使用该乳液替代脂肪,当替代量为75%时,鸡肉饼的煎制损失率降至最低。随替代量的增加,鸡肉饼的水分含量逐渐升高,蛋白质含量显著增加,同时脂肪含量降低。此外,添加SPI-XG乳液使得鸡肉饼的硬度下降,内聚性提高,L^(*)值升高,a^(*)和b^(*)值有所下降。总之,SPI-XG乳液作为一种脂肪替代物,能有效提升鸡肉饼的营养价值和烹饪性能,为未来脂肪替代物的开发提供了参考。

表达鸡传染性贫血病毒VP1蛋白的新型重组血清4型禽腺病毒的构建

为研制新型鸡传染性贫血(CIA)高效疫苗以解决国内商品鸡群中普遍存在的鸡传染性贫血病毒(CIAV)感染问题,试验利用CRISPR-Cas9技术和Cre-LoxP系统,以血清4型禽腺病毒(FAdV-4)为载体,构建表达CIAV VP1蛋白的重组FAdV-4。通过间接免疫荧光试验和蛋白免疫印迹试验对重组病毒进行验证,通过体外病毒生长曲线来研究其复制效率。结果显示:该重组病毒构建成功,能有效表达CIAV VP1蛋白,命名为rFAdV-4-CIAV-VP1,且发现rFAdV-4-CIAV-VP1在LMH细胞中的复制效率远低于野生型FAdV-4。研究表明构建的重组病毒rFAdV-4-CIAV-VP1为CIAV和FAdV-4的联防联控提供了二联候选疫苗株。

低蛋白日粮添加晶体氨基酸对21~60日龄青脚麻母鸡生长性能、血清生化指标和屠宰性能的影响

文章旨在研究低蛋白质日粮添加6种晶体氨基酸(赖氨酸、苏氨酸、色氨酸、精氨酸、缬氨酸、异亮氨酸)对青脚麻母鸡生长性能、血清生化指标和屠宰性能的影响。试验将720只21日龄的青脚麻母鸡分成对照组和试验组,每组6个重复,每个重复60只,21~40 d对照组和试验组粗蛋白质水平分别为19.5%、16.5%,D-lys为1%,按照理想氨基酸模型比例补充6种氨基酸(赖氨酸、苏氨酸、色氨酸、精氨酸、缬氨酸、异亮氨酸)达到一致水平。41~60 d对照组和试验组粗蛋白质水平分别为18%、15%,D-lys为0.85%,按照理想氨基酸模型比例补充6种氨基酸(赖氨酸、苏氨酸、色氨酸、精氨酸、缬氨酸、异亮氨酸)达到一致水平。结果表明,与对照组相比,试验组青脚麻母鸡的生长性能、血清生化指标和屠宰性能均无显著变化(P>0.05)。综上所述,在理想氨基酸模型下日粮中粗蛋白水平降低3%,21~60日龄青脚麻母鸡的生长性能、血清生化指标和屠宰性能均不受影响。