The aim of present investigation is to study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. The bioactivities of blood and spleen T lym...The aim of present investigation is to study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. The bioactivities of blood and spleen T lymphocytes including lymphoproliferation responses, cytotoxicitic responses, and histopathology of spleen were detected in broiler chickens singly- or co-infected with REV and ALV-J at different days post inoculation and the virus expressions in spleen of infected broiler chickens were detected with immunofluorescence assay (IFA). The results indicated that blood and spleen T lymphocytes proliferation responses and cytotoxicity in broilers infected with REV or/and ALV-J were inhibited in the whole observed period compared with controls. In the co-infected chickens they were highly inhibited than in the single-infected. The histopathology of spleen in infected chickens at 17 and 37 d post inoculation (dpi) indicated that cell interium increased, the numbers of lymphocytes decreased, and the regrowth were destroyed or decreased, especially more significantly at 17 than at 37 dpi. The different numbers of virus were detected in spleen lymphocytes in REV- infected and/or ALV-J-infected chickens. In the spleen of co-infected chicken, both REV and ALV-J were detected and the total numbers of viruses were more than in chickens singly-infected with REV or ALV-J. Thus, the co-effect of REV and ALV-J caused more immunosuppression on T lymphocytes bioactivities in broiler chickens than single-effect of ALV-J or REV, which contributed to the sever histopathology and the product of tumor cells. This study will be helpful for understanding the effect of co-infection with many viruses and control them in poultry.展开更多
Reticuloendotheliosis virus(REV)causes the atrophy of immune organs and immuno-suppression in chickens,but the underlying molecular mechanism of the immune response after infection by REV is not well understood.Presen...Reticuloendotheliosis virus(REV)causes the atrophy of immune organs and immuno-suppression in chickens,but the underlying molecular mechanism of the immune response after infection by REV is not well understood.Presently,the RNA-seq was used to analyze the regulation of immune response to REV in chicken lymphocytes from peripheral blood.Overall,134 differentially expressed long non-coding RNAs(lncRNAs)between cells with REV infection or without in vitro were screened.Based on the differentially expressed protein-coding genes,the nucleotide-binding oligomerization domain(NOD)-like receptor pathway related to immune regulation was enriched.Two lncRNAs(L11530 and L09863)were predicted to target the NOD1 and tumor necrosis factor receptor-associated factor 5(TRAF5)gene,respectively,which are involved in the NOD-like receptor pathway with cis-regulation way.The in vitro results revealed the significantly up-regulated(P<0.01)levels of lncRNA-L11530 and its target gene,NOD1,and the significantly down-regulated(P<0.05)levels of lncRNA-L09863 and its target gene,TRAF5,in lymphocytes after REV infection.These changes also occurred in vivo in blood lymphocytes of chickens infected with REV.Further,L09863 and L11530 were respectively interfered,the expression levels of their target genes NOD1 or TRAF5 were significantly down-regulated,accompanied by the change of IL-8 and IL-18 secretions in lymphocytes.The NOD-like receptor pathway appears to be important in the immune response to REV,LncRNA-11530 and lncRNA-09863 might involve in the immune regulation on REV infection by targeting NOD1 or TRAF5 in blood lymphocytes of chickens.Our findings reveal a new regulation of lncRNAs(L11530 and L09863)on immunity in chicken peripheral blood lymphocytes for REV infection by changing the expression of the target genes via the NOD-like receptor pathway.展开更多
Experimental study was conducted to examine the effect of copper excess on the peripheral blood T-lymphocyte by the methods of flow cytometry (FCM) and experimental pathology.420 one-day-old Avianchickens were randoml...Experimental study was conducted to examine the effect of copper excess on the peripheral blood T-lymphocyte by the methods of flow cytometry (FCM) and experimental pathology.420 one-day-old Avianchickens were randomly divided into seven groups,and fed on diets as follows:1.controls (Cu 11mg/kg)and 2.copper excess(Cu 100mg/kg,copper excess group Ⅰ;Cu 200mg/kg,copper excess group Ⅱ;Cu300mg/kg,copper excess group Ⅲ;Cu 400mg/kg,copper excess group Ⅳ;Cu 500mg/kg,copper excessgroup Ⅴ;Cu 600mg/kg,copper excess group Ⅵ) for six weeks.The results were as follows:1) In thy-mus,lymphocytes in the medulla were decreased in number in copper excess groups Ⅲ,Ⅳ,Ⅴ and Ⅵ,and the increased and enlarged thymic corpuscles and the proliferated reticular cells were also observed inboth copper excess group Ⅴ and copper excess group Ⅵ in comparison with those of control group.2) Thepercentage of CD_(4+) T cells was markedly decreased from 2 to 6 weeks of age in copper excess groups Ⅳ,Ⅴ and Ⅵ (P<0.05 or P<0.01).3) The percentage of CD_(8+) T cell was not varied in six copper ex-cess groups during the experiment when compared with that of control group (P>0.05).4) The CD_(4+)/CD_(8+) ratio was lower from 2 to 6 weeks of age in copper excess groups Ⅳ,Ⅴ and Ⅵ than in control group(P<0.05 or P<0.01).5) It was concluded that dietary copper in excess of 300mg/kg suppressed thedevelopment of T-lymphocytes and reduced the percentage of CD_(4+) T cells and the CD_(4+)/CD_(8+) ratio,andresulted in pathological injury of the thymus.Cellular immune function was finally impaired.展开更多
Cytochrome c is a component of mitochondrial respiratory chain, located at the outer side of mitochondrial inner membrane. Its precursor, apocytochrome c, is encoded by a nuclear gene, synthesized on cytoplasmic ribos...Cytochrome c is a component of mitochondrial respiratory chain, located at the outer side of mitochondrial inner membrane. Its precursor, apocytochrome c, is encoded by a nuclear gene, synthesized on cytoplasmic ribosomes, and posttranslationally imported into mitochondria, but apocytochrome c is unique in the translocation compared with most mitochondrial proteins. It does not carry a cleavable amino terminal targeting sequence; no proteinous receptor on the mitochondrial outer membrane is identified for its import and its translocation does not compete with other preproteins for translocation machinery in the outer membrane. Besides, neither ATP nor membrane potential is required for its translocation across mitochonctria.展开更多
A series of artificial membranes called small unilaminar vesicles (SUVs) comprising different molar ratios of phosphatidylcholine (PC)/cholesterol (Ch) were constructed and exogenous PC and Ch were transferred into hu...A series of artificial membranes called small unilaminar vesicles (SUVs) comprising different molar ratios of phosphatidylcholine (PC)/cholesterol (Ch) were constructed and exogenous PC and Ch were transferred into human peripheral blood lymphocytes through incubating the cells with SUVs in order to study the effects of the changed constituents of membrane lipids on the expression of CD 71 in lymphocytes, the binding characteristics of CD 71 molecules with its ligand and its internalization. It was found that after the treatment of SUVs, the Ch content in the plasmic membrane was increased with a decrease of PC/Ch ratio. After incubation with ConA for 48 h,the percentage of CD 71-positive lymphocytes increased with the increase of the availab1e binding sites for CD 71 to its ligand, but the affinity of CD 71 with its ligand was lowered- When Ch synthesis was blocked with HMG-CoAreductase inhibitor (Lovastatin), the number of CD71 molecules was obviously reducce but the afinity of CD 71with its ligand elevated- It is believed that that the construction of the lipid environment can directly affect the properties of CD 71 molecules. Less fluid phase of the lipid environment might be suitable to the move;nent of CD 71 in the membrane but minimize the tendency for CD 71 to maintain a con formation of high affinity state.展开更多
Studied features of cellular and humoral factors of immune system and activation markers of lymphocytes at breast cancer patients.Analyzed biophysical parameters of lymphocyte superficial membranes of periphery blood ...Studied features of cellular and humoral factors of immune system and activation markers of lymphocytes at breast cancer patients.Analyzed biophysical parameters of lymphocyte superficial membranes of periphery blood of breast cancer patients.Features of immune system reaction were:expressed Т - cellular immunodeficiency,deficiency of CD4+Т-helpers/inducers against raised expression of СD8+Т-lymphocyte,suppression of immunoregulatory index,imbalance of immunity humoral link,activity of expression CD16 + and CD56 + on natural killers and rising of expression CD38 + and CD95 + on lymphocytes.Studying of biophysical parameters of lymphocytes superficial membranes has allowed to reveal statistically significant changes characterized by intensifying of electric field and rising ofmicroviscosity,obviously leading to disappearance or weakening of intercellular interactions.展开更多
AIM: To disclose the mechanisms that accelerate or limit tumor invasion and metastasis in gastric cancer patients. METHODS: The heparanase expression,continuity of basement,degree of infiltration by dendritic cells an...AIM: To disclose the mechanisms that accelerate or limit tumor invasion and metastasis in gastric cancer patients. METHODS: The heparanase expression,continuity of basement,degree of infiltration by dendritic cells and lymphocytes in gastric cancer tissues from 33 the early and late stage patients were examined by immunohistochemistry,in situ hybridization and transmission electron microscopy. RESULTS: Heparanase mRNA expression in the late stage patients with gastric cancer was stronger than that in the early stage gastric cancer patients. In the early stage gastric cancer tissues,basement membrane (BM) appeared intact,whereas in the late stage,discontinuous BM was often present. The density of S100 protein positive tumor infi ltrating dendritic cells (TIDC) in the early stage gastric cancer tissues was higher than that in the late stage. The infiltrating degree of tumor infi ltrating lymphocytes (TIL) in the early stage patients whose tumor tissues contained a high density of TIDC was signifi cantly higher than that in the late stage gastric cancer tissues patients with a low density of TIDC. There were few cancer cells penetrated through the continuous BM of cancer nests in the early stage gastric cancers,but many cancer cells were found outside of the defective BM of cancer nests in the late stage. CONCLUSION: Our results suggest that strongheparanase expression is related with the degradation of BM which allows or accelerates tumor invasion and metastasis. However,high density of TIDC and degree of infi ltration by TIL are associated with tumor progression in human gastric cancers.展开更多
Lymphocytes respond to mitogens that stimulate proliferation by increasing theirs metabolic activity. In this study we investigate L-Glutamine and L-Leucine uptake as markers of cell response to Concavalina A (ConcaA)...Lymphocytes respond to mitogens that stimulate proliferation by increasing theirs metabolic activity. In this study we investigate L-Glutamine and L-Leucine uptake as markers of cell response to Concavalina A (ConcaA) stimulation, using a high-resolution flow technique. We found that lymphocytes induced to blast transformation enhanced rate and efficiency of amino acid uptake during cell proliferation. Considering that increases in transport is the first quantifiable response of cells during malignant transformation, amino acid uptake could also be useful as an early marker of malignancy.展开更多
基金supported by the Special Fund for Agroscientific Research in the Public Interest, China(200803019)the Youth Innovation Foudation of Shandong Agricultural University, China (23477)
文摘The aim of present investigation is to study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. The bioactivities of blood and spleen T lymphocytes including lymphoproliferation responses, cytotoxicitic responses, and histopathology of spleen were detected in broiler chickens singly- or co-infected with REV and ALV-J at different days post inoculation and the virus expressions in spleen of infected broiler chickens were detected with immunofluorescence assay (IFA). The results indicated that blood and spleen T lymphocytes proliferation responses and cytotoxicity in broilers infected with REV or/and ALV-J were inhibited in the whole observed period compared with controls. In the co-infected chickens they were highly inhibited than in the single-infected. The histopathology of spleen in infected chickens at 17 and 37 d post inoculation (dpi) indicated that cell interium increased, the numbers of lymphocytes decreased, and the regrowth were destroyed or decreased, especially more significantly at 17 than at 37 dpi. The different numbers of virus were detected in spleen lymphocytes in REV- infected and/or ALV-J-infected chickens. In the spleen of co-infected chicken, both REV and ALV-J were detected and the total numbers of viruses were more than in chickens singly-infected with REV or ALV-J. Thus, the co-effect of REV and ALV-J caused more immunosuppression on T lymphocytes bioactivities in broiler chickens than single-effect of ALV-J or REV, which contributed to the sever histopathology and the product of tumor cells. This study will be helpful for understanding the effect of co-infection with many viruses and control them in poultry.
基金supported by the National Natural Science Foundation of China (31802057)the National Key Technology R&D Program of China (2015BAD03B03)
文摘Reticuloendotheliosis virus(REV)causes the atrophy of immune organs and immuno-suppression in chickens,but the underlying molecular mechanism of the immune response after infection by REV is not well understood.Presently,the RNA-seq was used to analyze the regulation of immune response to REV in chicken lymphocytes from peripheral blood.Overall,134 differentially expressed long non-coding RNAs(lncRNAs)between cells with REV infection or without in vitro were screened.Based on the differentially expressed protein-coding genes,the nucleotide-binding oligomerization domain(NOD)-like receptor pathway related to immune regulation was enriched.Two lncRNAs(L11530 and L09863)were predicted to target the NOD1 and tumor necrosis factor receptor-associated factor 5(TRAF5)gene,respectively,which are involved in the NOD-like receptor pathway with cis-regulation way.The in vitro results revealed the significantly up-regulated(P<0.01)levels of lncRNA-L11530 and its target gene,NOD1,and the significantly down-regulated(P<0.05)levels of lncRNA-L09863 and its target gene,TRAF5,in lymphocytes after REV infection.These changes also occurred in vivo in blood lymphocytes of chickens infected with REV.Further,L09863 and L11530 were respectively interfered,the expression levels of their target genes NOD1 or TRAF5 were significantly down-regulated,accompanied by the change of IL-8 and IL-18 secretions in lymphocytes.The NOD-like receptor pathway appears to be important in the immune response to REV,LncRNA-11530 and lncRNA-09863 might involve in the immune regulation on REV infection by targeting NOD1 or TRAF5 in blood lymphocytes of chickens.Our findings reveal a new regulation of lncRNAs(L11530 and L09863)on immunity in chicken peripheral blood lymphocytes for REV infection by changing the expression of the target genes via the NOD-like receptor pathway.
基金the National Natural Science Foundation of China(No.30471304)the Sichuan Education Department(2002A002)Sichuan Scientific & Technical Department(03JY029-030-2)
文摘Experimental study was conducted to examine the effect of copper excess on the peripheral blood T-lymphocyte by the methods of flow cytometry (FCM) and experimental pathology.420 one-day-old Avianchickens were randomly divided into seven groups,and fed on diets as follows:1.controls (Cu 11mg/kg)and 2.copper excess(Cu 100mg/kg,copper excess group Ⅰ;Cu 200mg/kg,copper excess group Ⅱ;Cu300mg/kg,copper excess group Ⅲ;Cu 400mg/kg,copper excess group Ⅳ;Cu 500mg/kg,copper excessgroup Ⅴ;Cu 600mg/kg,copper excess group Ⅵ) for six weeks.The results were as follows:1) In thy-mus,lymphocytes in the medulla were decreased in number in copper excess groups Ⅲ,Ⅳ,Ⅴ and Ⅵ,and the increased and enlarged thymic corpuscles and the proliferated reticular cells were also observed inboth copper excess group Ⅴ and copper excess group Ⅵ in comparison with those of control group.2) Thepercentage of CD_(4+) T cells was markedly decreased from 2 to 6 weeks of age in copper excess groups Ⅳ,Ⅴ and Ⅵ (P<0.05 or P<0.01).3) The percentage of CD_(8+) T cell was not varied in six copper ex-cess groups during the experiment when compared with that of control group (P>0.05).4) The CD_(4+)/CD_(8+) ratio was lower from 2 to 6 weeks of age in copper excess groups Ⅳ,Ⅴ and Ⅵ than in control group(P<0.05 or P<0.01).5) It was concluded that dietary copper in excess of 300mg/kg suppressed thedevelopment of T-lymphocytes and reduced the percentage of CD_(4+) T cells and the CD_(4+)/CD_(8+) ratio,andresulted in pathological injury of the thymus.Cellular immune function was finally impaired.
基金Project supported by the National Natural Science Foundation of China (Grant No. 39730130).
文摘Cytochrome c is a component of mitochondrial respiratory chain, located at the outer side of mitochondrial inner membrane. Its precursor, apocytochrome c, is encoded by a nuclear gene, synthesized on cytoplasmic ribosomes, and posttranslationally imported into mitochondria, but apocytochrome c is unique in the translocation compared with most mitochondrial proteins. It does not carry a cleavable amino terminal targeting sequence; no proteinous receptor on the mitochondrial outer membrane is identified for its import and its translocation does not compete with other preproteins for translocation machinery in the outer membrane. Besides, neither ATP nor membrane potential is required for its translocation across mitochonctria.
文摘A series of artificial membranes called small unilaminar vesicles (SUVs) comprising different molar ratios of phosphatidylcholine (PC)/cholesterol (Ch) were constructed and exogenous PC and Ch were transferred into human peripheral blood lymphocytes through incubating the cells with SUVs in order to study the effects of the changed constituents of membrane lipids on the expression of CD 71 in lymphocytes, the binding characteristics of CD 71 molecules with its ligand and its internalization. It was found that after the treatment of SUVs, the Ch content in the plasmic membrane was increased with a decrease of PC/Ch ratio. After incubation with ConA for 48 h,the percentage of CD 71-positive lymphocytes increased with the increase of the availab1e binding sites for CD 71 to its ligand, but the affinity of CD 71 with its ligand was lowered- When Ch synthesis was blocked with HMG-CoAreductase inhibitor (Lovastatin), the number of CD71 molecules was obviously reducce but the afinity of CD 71with its ligand elevated- It is believed that that the construction of the lipid environment can directly affect the properties of CD 71 molecules. Less fluid phase of the lipid environment might be suitable to the move;nent of CD 71 in the membrane but minimize the tendency for CD 71 to maintain a con formation of high affinity state.
文摘Studied features of cellular and humoral factors of immune system and activation markers of lymphocytes at breast cancer patients.Analyzed biophysical parameters of lymphocyte superficial membranes of periphery blood of breast cancer patients.Features of immune system reaction were:expressed Т - cellular immunodeficiency,deficiency of CD4+Т-helpers/inducers against raised expression of СD8+Т-lymphocyte,suppression of immunoregulatory index,imbalance of immunity humoral link,activity of expression CD16 + and CD56 + on natural killers and rising of expression CD38 + and CD95 + on lymphocytes.Studying of biophysical parameters of lymphocytes superficial membranes has allowed to reveal statistically significant changes characterized by intensifying of electric field and rising ofmicroviscosity,obviously leading to disappearance or weakening of intercellular interactions.
文摘目的 探究芪地桃蛭二蝉方联合他克莫司治疗膜性肾病的效果及对抗磷脂酶A2受体(PLA2R)、B淋巴细胞活化因子(BAFF)及白细胞介素-6(IL-6)水平的影响。方法 选取2017年11月至2020年4月收治的84例膜性肾病患者为研究对象,将其随机分为对照组与联合组,各42例。对照组给予基础治疗加他克莫司治疗,联合组在对照组基础上加芪地桃蛭二蝉方治疗。比较两组的治疗效果。结果 联合组的治疗总有效率高于对照组,差异具有统计学意义(P<0.05)。治疗前,两组的24 h尿蛋白定量(24 h UPQ)、人血清白蛋白(HSA)、血清肌酐浓度(Scr)水平比较,差异无统计学意义(P>0.05);治疗后,两组的24 h UPQ、Scr水平均明显降低,HSA水平均明显升高,且联合组优于对照组,差异具有统计学意义(P<0.05)。治疗前,两组的PLA2R、BAFF及IL-6水平比较,差异无统计学意义(P>0.05);治疗后,两组的PLA2R、BAFF及IL-6水平均明显降低,且联合组低于对照组,差异具有统计学意义(P<0.05)。联合组的不良反应总发生率低于对照组,差异具有统计学意义(P<0.05)。结论 芪地桃蛭二蝉方联合他克莫司治疗膜性肾病效果显著,能够明显降低PLA2R、BAFF及IL-6水平,安全可靠,值得推广。
文摘AIM: To disclose the mechanisms that accelerate or limit tumor invasion and metastasis in gastric cancer patients. METHODS: The heparanase expression,continuity of basement,degree of infiltration by dendritic cells and lymphocytes in gastric cancer tissues from 33 the early and late stage patients were examined by immunohistochemistry,in situ hybridization and transmission electron microscopy. RESULTS: Heparanase mRNA expression in the late stage patients with gastric cancer was stronger than that in the early stage gastric cancer patients. In the early stage gastric cancer tissues,basement membrane (BM) appeared intact,whereas in the late stage,discontinuous BM was often present. The density of S100 protein positive tumor infi ltrating dendritic cells (TIDC) in the early stage gastric cancer tissues was higher than that in the late stage. The infiltrating degree of tumor infi ltrating lymphocytes (TIL) in the early stage patients whose tumor tissues contained a high density of TIDC was signifi cantly higher than that in the late stage gastric cancer tissues patients with a low density of TIDC. There were few cancer cells penetrated through the continuous BM of cancer nests in the early stage gastric cancers,but many cancer cells were found outside of the defective BM of cancer nests in the late stage. CONCLUSION: Our results suggest that strongheparanase expression is related with the degradation of BM which allows or accelerates tumor invasion and metastasis. However,high density of TIDC and degree of infi ltration by TIL are associated with tumor progression in human gastric cancers.
文摘Lymphocytes respond to mitogens that stimulate proliferation by increasing theirs metabolic activity. In this study we investigate L-Glutamine and L-Leucine uptake as markers of cell response to Concavalina A (ConcaA) stimulation, using a high-resolution flow technique. We found that lymphocytes induced to blast transformation enhanced rate and efficiency of amino acid uptake during cell proliferation. Considering that increases in transport is the first quantifiable response of cells during malignant transformation, amino acid uptake could also be useful as an early marker of malignancy.