Different in-vitro and Clinical Studies to Evaluate the Improvement of the Eyes Cream Containing the Natural Algal Complex

A natural algal complex is prepared from the brown alga Undaria pinnatifida rich in fucoidan and the red calcareous algal Corallina officinalis. The effect of the algal complex was demonstrated by transcriptomic analysis on normal human fibroblasts through the DNA chip technology from AFFYMETRIX, combined with the following in vitro Elisa test and clinical studies. Clinical studies have been performed with a basic cream containing complex versus placebo on 2 groups of 30 Caucasian women for a period of 28 days. In the present study, the natural algal complex works on the crow’s feet, eye bags, and dark cycle through multiple ways of action, including enhancing natural immune responses, regulating the inflammatory and immunity process, and promoting the extracellular matrix synthesis. As the natural algal complex has excellent improvement on the eye circumference, we have applied it to mageline firming anti-wrinkle eye cream for further research.

A Rapid Processing Technology of Water Quality Microfluidic Chip

In this study,a unique rapid processing technology for microfluidic chips made of polymethylmethacrylate(PMMA)was realized.The common laser engraving machine is used to etch the chip reaction unit and microchannel,and the printing chip is what you see is what you get;the pressurized heat sealing technology is used to quickly complete the chip sealing and packaging;the metal electrodes and lines that need to be laid are embedded into the chip by hot melting;the contact points on the fixed base of the contact chip card are used to connect with the external equipment,so as to make the bearing sample react,detect and complex lines and chips.It is self-contained and isolated from the external equipment circuit.The chip becomes disposable and can be replaced quickly and conveniently.The chip processed by this technology has rapid,cheap and convenient improvement and innovation,which makes the production of microfluidic chip easier and more practical,and even makes the chip a disposable consumable with general interface,which will greatly promote the industrialization of microfluidic chip,and has market-oriented promotion value in the field of water quality and food detection.

Characteristic gene expression profiles in the progression from normal gastric epithelial cells to moderate gastric epithelial dysplasia and to gastric cancer

Background Gastric cancer ranks high among the most common causes of cancer-related death worldwide. This study was designed to explore key genes involved in the progression of normal gastric epithelial cells to moderate gastric epithelial dysplasia （mGED） and to gastric cancer. Methods Twelve pairs of mGED tissues, gastric cancer tissues, and normal gastric tissues were collected by gastroscopy. Total RNA was then extracted and purified. After the addition of fluorescent tags, hybridization was carried out on a Gene chip microarray slide. Significance analysis of microarrays was performed to determine significant differences in gene expression between the different tissue types. Results Microarray data analysis revealed totally 34 genes that were expressed differently： 18 highly expressed （fold change 〉2; P 〈0.01） and 16 down-regulated （fold change 〉2; P 〈0.01）. Of the 34 genes, 24 belonged to several different functional categories such as structural molecule activity, extracellular regions, structural formation, cell death, biological adhesion, developmental processes, locomotion, and biological regulation that were associated with cancer. The remaining 10 genes were not involved in cancer research. Of these genes, the expression levels of Matrix metalloproteinase-12 （MMP12）, Caspase-associated recruitment domain 14 （CARD14）, and Chitinase 3-like 1 （CHI3L1） were confirmed by semi-quantitative RT-PCR. A two-way clustering algorithm divided the 36 samples into three categories and the overall correct classification efficiency was 80.6% （29/36）. Almost all of these genes （31/34） showed constant changes in the process of normal gastric epithelial cells to mGED to gastric cancer. Conclusions The results of this study provided global gene expression profiles during the development and progression from normal gastric epithelial cells to mGED to gastric cancer. These data may provide new insights into the molecular pathology of gastric cancer which may be useful for the detection, diagnosis, and treatment.