The effect of foliar spray of young lemon trees with Chitosan (CH) (200 - 300 - 400 ppm), on antioxidant enzymes activities (peroxidase POD, polyphenol oxidase PPO) and total phenolic content, and their influence on l...The effect of foliar spray of young lemon trees with Chitosan (CH) (200 - 300 - 400 ppm), on antioxidant enzymes activities (peroxidase POD, polyphenol oxidase PPO) and total phenolic content, and their influence on leaf miner (Phyllocnistis citrella) activity (tunnel length and damage density) under field condition was investigated. The results showed that treatment with chitosan 300 ppm enhanced the total phenolic content (5.975 mg/g) and the POD activity (0.533 min<sup>-1</sup> mg<sup>-1</sup> protein), while PPO activity was increased by chitosan 200 ppm (1.394 ΔA/min/g FW). The results demonstrated as well that chitosan treatment has a beneficial effect in reducing leaf miner activity, by decreasing tunnel length and damage density.展开更多
AIM:To establish a reliable method for assay of chitosan.METHODS:Spectrophotometry of a resultant green complex between bromocresol green and chitosan was systemically evaluated for the determination of chitosan.RESUL...AIM:To establish a reliable method for assay of chitosan.METHODS:Spectrophotometry of a resultant green complex between bromocresol green and chitosan was systemically evaluated for the determination of chitosan.RESULTS:The reaction between bromocresol green and chitosan arrived its end in 5 min at 37 ℃.The formed complex showed maximal absorption wavelength of 620 nm which could remain stable in 2 hours.The assay indicated a linear range up to 10.0 g/L with a correlation coefficient of 0.999 6.Coefficients of variation of the assay at low,middle,and high levels were 0.81%,0.78% and 0.26%,respectively.The averaged recovery was 99.17%.It was also found that the cell wasn′t stained with the pigments and there was no cross-over containment.However,interferences resulted from surfactant,metalion,and albumin.CONCLUSION:This method was reliable,simple,and rapid.It could meet the need of chitosan determination.展开更多
文摘The effect of foliar spray of young lemon trees with Chitosan (CH) (200 - 300 - 400 ppm), on antioxidant enzymes activities (peroxidase POD, polyphenol oxidase PPO) and total phenolic content, and their influence on leaf miner (Phyllocnistis citrella) activity (tunnel length and damage density) under field condition was investigated. The results showed that treatment with chitosan 300 ppm enhanced the total phenolic content (5.975 mg/g) and the POD activity (0.533 min<sup>-1</sup> mg<sup>-1</sup> protein), while PPO activity was increased by chitosan 200 ppm (1.394 ΔA/min/g FW). The results demonstrated as well that chitosan treatment has a beneficial effect in reducing leaf miner activity, by decreasing tunnel length and damage density.
文摘AIM:To establish a reliable method for assay of chitosan.METHODS:Spectrophotometry of a resultant green complex between bromocresol green and chitosan was systemically evaluated for the determination of chitosan.RESULTS:The reaction between bromocresol green and chitosan arrived its end in 5 min at 37 ℃.The formed complex showed maximal absorption wavelength of 620 nm which could remain stable in 2 hours.The assay indicated a linear range up to 10.0 g/L with a correlation coefficient of 0.999 6.Coefficients of variation of the assay at low,middle,and high levels were 0.81%,0.78% and 0.26%,respectively.The averaged recovery was 99.17%.It was also found that the cell wasn′t stained with the pigments and there was no cross-over containment.However,interferences resulted from surfactant,metalion,and albumin.CONCLUSION:This method was reliable,simple,and rapid.It could meet the need of chitosan determination.
