Objective To study the regulating effects of a novel CpG oligodeoxynuleotide and the synergistic effect of chitosan-nanoparticles （CNP） with CpG on immune responses of mice, which were used to develop a novel immuno...Objective To study the regulating effects of a novel CpG oligodeoxynuleotide and the synergistic effect of chitosan-nanoparticles （CNP） with CpG on immune responses of mice, which were used to develop a novel immunoadjuvant to boost immune response to conventional vaccines. Methods A novel CpG ODN containing 11 CpG motifs was synthesized and its bioactivities to stimulate the proliferation of lymphocytes of pig in vitro were detected. Then it was entrapped with CNP prepared in our laboratory by the method of ionic cross linkage, and immunized Kunming mice were co-inoculated with paratyphoid vaccine. The peripheral blood was collected weekly from the tail vein of inoculated mice to detect the contents of IgG, IgA, IgM, and specific antibody against salmonella as well as the levels of interleukin-2 （IL2）, IL-4, and IL-6 by SABC-ELISA assay. The numbers of leucocytes, monocytes, granuloytes, and lymphocytes were calculated separately using the routine method. The experimental mice were orally challenged with virulent salmonella 35 days after inoculation. Results This CpG ODN could remarkably provoke the proliferation of lymphocytes of pig in vitro in contrast with the control （P〈0.05）. Compared with those of the control, immunoglobulins, including IgG, IgA, IgM, and specific antibodies to paratyphoid vaccine, increased significantly in sera from the CpG or CpG-CNP-vaccinated mice （P〈0.05）. IL-2, IL-4, and IL-6 increased remarkably in sera from immunized mice （P〈0.05）. The leucocytes, monocytes, granuloytes, and lymphocytes of the mice immunized with CpG or CpG-CNP were also increased in number （P〈0.05）. After the challenge, these immunity values were elevated in the mice vaccinated with CpG or CpG-CNP. The immunized mice all survived, while the control mice fell ill with evident lesions with diffuse hemorrhage in stomach, small intestine, and peritoneum. Conclusions CpG ODN entrapped with CNP is a promising effective immunoadjuvant for vaccination, which promotes humoral and cellular immune responses, enhances immunity and resistance against salmonella by co-administration with paratyphoid vaccine. Key words：展开更多
目的探讨高浓度葡萄糖(简称高糖)对C型钠尿肽(CNP)致胃窦环形肌舒张的影响及其可能机制。方法取大鼠40只制备胃窦环形肌条,肌条收缩活动稳定后,对照1、2组分别加入不同浓度CNP(1×10-8、3×10-8、1×10-7mol/L)及硝普钠(SNP...目的探讨高浓度葡萄糖(简称高糖)对C型钠尿肽(CNP)致胃窦环形肌舒张的影响及其可能机制。方法取大鼠40只制备胃窦环形肌条,肌条收缩活动稳定后,对照1、2组分别加入不同浓度CNP(1×10-8、3×10-8、1×10-7mol/L)及硝普钠(SNP)(1×10-7、3×10-7、1×10-6mol/L),观察其效应,每次加药观察20 m in,冲洗3次;高糖1、2组预加葡萄糖使浴槽终浓度为30 mmol/L,其后操作同对照1、2组;甘露醇组预加甘露醇30mmol/L,其后操作同对照1组;均采用多道生理信号采集处理系统计算CNP(SNP)致平滑肌张力的抑制率。结果对照1、2组产生浓度依赖的胃窦环形肌平滑肌舒张效应,高糖1组可明显抑制CNP和硝普钠产生的平滑肌条舒张效应明显减弱;甘露醇(30 mmol/L)组CNP产生的平滑肌条舒张效应明显减弱。结论高糖抑制CNP引起的胃窦环形肌舒张效应;该效应可能与细胞内外渗透压改变有关。展开更多
文摘Objective To study the regulating effects of a novel CpG oligodeoxynuleotide and the synergistic effect of chitosan-nanoparticles （CNP） with CpG on immune responses of mice, which were used to develop a novel immunoadjuvant to boost immune response to conventional vaccines. Methods A novel CpG ODN containing 11 CpG motifs was synthesized and its bioactivities to stimulate the proliferation of lymphocytes of pig in vitro were detected. Then it was entrapped with CNP prepared in our laboratory by the method of ionic cross linkage, and immunized Kunming mice were co-inoculated with paratyphoid vaccine. The peripheral blood was collected weekly from the tail vein of inoculated mice to detect the contents of IgG, IgA, IgM, and specific antibody against salmonella as well as the levels of interleukin-2 （IL2）, IL-4, and IL-6 by SABC-ELISA assay. The numbers of leucocytes, monocytes, granuloytes, and lymphocytes were calculated separately using the routine method. The experimental mice were orally challenged with virulent salmonella 35 days after inoculation. Results This CpG ODN could remarkably provoke the proliferation of lymphocytes of pig in vitro in contrast with the control （P〈0.05）. Compared with those of the control, immunoglobulins, including IgG, IgA, IgM, and specific antibodies to paratyphoid vaccine, increased significantly in sera from the CpG or CpG-CNP-vaccinated mice （P〈0.05）. IL-2, IL-4, and IL-6 increased remarkably in sera from immunized mice （P〈0.05）. The leucocytes, monocytes, granuloytes, and lymphocytes of the mice immunized with CpG or CpG-CNP were also increased in number （P〈0.05）. After the challenge, these immunity values were elevated in the mice vaccinated with CpG or CpG-CNP. The immunized mice all survived, while the control mice fell ill with evident lesions with diffuse hemorrhage in stomach, small intestine, and peritoneum. Conclusions CpG ODN entrapped with CNP is a promising effective immunoadjuvant for vaccination, which promotes humoral and cellular immune responses, enhances immunity and resistance against salmonella by co-administration with paratyphoid vaccine. Key words：
文摘目的探讨高浓度葡萄糖(简称高糖)对C型钠尿肽(CNP)致胃窦环形肌舒张的影响及其可能机制。方法取大鼠40只制备胃窦环形肌条,肌条收缩活动稳定后,对照1、2组分别加入不同浓度CNP(1×10-8、3×10-8、1×10-7mol/L)及硝普钠(SNP)(1×10-7、3×10-7、1×10-6mol/L),观察其效应,每次加药观察20 m in,冲洗3次;高糖1、2组预加葡萄糖使浴槽终浓度为30 mmol/L,其后操作同对照1、2组;甘露醇组预加甘露醇30mmol/L,其后操作同对照1组;均采用多道生理信号采集处理系统计算CNP(SNP)致平滑肌张力的抑制率。结果对照1、2组产生浓度依赖的胃窦环形肌平滑肌舒张效应,高糖1组可明显抑制CNP和硝普钠产生的平滑肌条舒张效应明显减弱;甘露醇(30 mmol/L)组CNP产生的平滑肌条舒张效应明显减弱。结论高糖抑制CNP引起的胃窦环形肌舒张效应;该效应可能与细胞内外渗透压改变有关。
