甲壳胺制剂对真鲷幼鱼的促生长作用研究

报道了甲壳胺制剂对真鲷幼鱼的良好促生长效果。试验中所用基础饵料的成分为鱼粉、小麦粉、豆粉等。在对真鲷幼鱼的试验中 ,在体重增长方面 ,甲壳胺制剂增重效果明显 ,增重率高达2 8.0 3% 。

壳聚糖碘液体外对乙型肝炎病毒灭活的实验研究

采用酶联免疫吸附实验(ELISA)和荧光定量PCR的方法检测壳聚糖碘液对血清HBsAg和HBV DNA的破坏作用。实验结果表明1.56250 g/L和0.78125 g/L的壳聚糖碘液分别作用5 m in和10 m in能完全破坏HBsAg的抗原性;6.25000 g/L和3.12500 g/L的壳聚糖碘液分别作用5 m in和15 m in能较好地破坏血清中的HBV DNA。提示壳聚糖碘液具有体外灭活乙型肝炎病毒作用。

虾壳综合加工工艺的研究

本文对虾仁的副产品-虾壳的综合加工工艺进行了初步探讨,用此工艺可获取甲壳素24%,壳聚糖22%,氨基葡萄糖盐酸盐13%,碳酸钙43%,蛋白粉4.4%。与文献相比较,具有一定优势。

壳聚糖对运动过氧化损伤的康复作用研究

目的 探讨补充壳聚糖对不同负荷运动大鼠骨骼肌自由基代谢的影响。方法 以 SD大鼠为实验对象，随机分成壳聚糖服用组和对照组。每大组群又分成安静组、一次性有氧运动组、一次性大强度运动组和连续运动组。处死后，测定比目鱼肌和腓肠肌的 SOD(超氧化物岐化酶 )MAD丙二醛。结果 1.比目鱼肌和腓肠在一次性有氧运动后即刻和一次性大强度运动后即刻， MDA值均较安静对照组有显著性升高 (P < 0.01)。 2.腓肠肌的脂质过氧化反应程度明显高于比目鱼肌；腓肠肌的 MDA与安静对照组相比高出 85.7％ (P< 0.01)。 3.连续运动＋服壳聚糖组的 MDA显著下降。结论 1.脂质过氧化加强，与运动强度有关。 SOD活性相对下降可能是运动后骨骼肌细胞脂质过氧化加强的原因之一。 2.不同骨骼肌脂质过氧化水平是不同的。 3.随着运动负荷加大，壳聚糖消除体内自由基的作用越明显。

Optimization and evaluation of microencapsulated artificial diet for mass rearing the predatory ladybird Propylea japonica （Coleoptera： Coccinellidae）

Artificial diet optimization is a key aspect in mass rearing of natural enemies since it influences the quality and feeding effectiveness, and thus the success of the biological control program. Here, we introduced the microencapsulation method to package liquid artificial diet for feeding of the ladybird Propyleajaponica. An orthogonal test of the quality of microencapsulated artificial diets （ADMs） was performed on key variables in production; Ca-alginate concentration, chitosan concentration and weight ratio of wall material to inner diet. We compared the development and reproduction of P. japonica fed on the ADMs under different cold-stored periods with those fed on fresh aphids and liquid artificial diets, in addition to a comparison of respiration, locomotion and predation. Our results indicated that chitosan concentration and ratio of shell to core significantly influence the quality of ADMs. The optimal recipe is 1.0% Ca-alginate, 1.6% chitosan and shell ： core = 1 ： 2. Insects reared on fresh optimized ADMs were similar to those fed on fresh prey in all developmental and reproductive characteristics except for survival ratio and female fertility. ADMs appeared more beneficial than using a liquid artificial diet, although this may decrease with the prolonging of the cold-storage period. P.japonica fed either on fresh ADMs or fresh prey showed improved respiration and predation abilities compared to where liquid artificial diet was used. Our study indicates advantages of microencapsulation in the production of artificial diet for predatory ladybird rearing. A microencapsulated diet can directly increase the efficiency and stability of mass rearing .

姜黄素壳聚糖微球的制备及其在体内外释放度的研究

目的:研究响应面法优化姜黄素壳聚糖微球制备的工艺参数,提高姜黄素的溶出度。方法:采用离子交联法制备姜黄素缓释微球,以微球的载药量和包封率为考察指标,采用星点设计考察配制壳聚糖的醋酸浓度、药物载体的比例以及交联剂浓度对微球制备工艺的影响,对结果进行二次多项式拟合,并根据最佳数学模型进行预测。结果:姜黄素壳聚糖微球最优制备工艺参数为:醋酸的浓度为1%,载体药物比例为0.83,交联剂的浓度为0.15%,载药量和包封率的预测值和理论值偏差分别为0.47%和3.2%。结论:响应面法优化姜黄素壳聚糖微球制剂处方具有很好的预测性,体内外药物释放度研究表明,最优条件下制备的微球可以在提高姜黄素溶出度的前提下缓慢释放达12 h。