Spatio-temporal variability of surface chlorophyll a in the Yellow Sea and the East China Sea based on reconstructions of satellite data of 2001-2020

Chlorophyll-a(Chl-a)concentration is a primary indicator for marine environmental monitoring.The spatio-temporal variations of sea surface Chl-a concentration in the Yellow Sea(YS)and the East China Sea(ECS)in 2001-2020 were investigated by reconstructing the MODIS Level 3 products with the data interpolation empirical orthogonal function(DINEOF)method.The reconstructed results by interpolating the combined MODIS daily+8-day datasets were found better than those merely by interpolating daily or 8-day data.Chl-a concentration in the YS and the ECS reached its maximum in spring,with blooms occurring,decreased in summer and autumn,and increased in late autumn and early winter.By performing empirical orthogonal function(EOF)decomposition of the reconstructed data fields and correlation analysis with several potential environmental factors,we found that the sea surface temperature(SST)plays a significant role in the seasonal variation of Chl a,especially during spring and summer.The increase of SST in spring and the upper-layer nutrients mixed up during the last winter might favor the occurrence of spring blooms.The high sea surface temperature(SST)throughout the summer would strengthen the vertical stratification and prevent nutrients supply from deep water,resulting in low surface Chl-a concentrations.The sea surface Chl-a concentration in the YS was found decreased significantly from 2012 to 2020,which was possibly related to the Pacific Decadal Oscillation(PDO).

Spatial correlations in time and frequency domains between chlorophyll-a concentration and environmental factors in the Bohai Sea

Based on the reconstructed MODIS data and ECMWF reanalysis data from 2003 to 2021,spatial correlations between chlorophyll a(Chl a)and sea surface temperature(SST),photosynthetically available radiation(PAR),aerosol optical thickness(AOT),and wind speed(WS)in the Bohai Sea were analyzed from the perspective of time domain and frequency domain.Results indicate that the frequency domain analysis was more conducive to revealing the correlations between Chl a and environmental factors.The spatial pattern of time-domain correlations was similar to the isobaths of the Bohai Sea,which was positive in shallow waters and negative in deep waters for SST,PAR,and AOT,and was reversed for WS.Frequency-domain correlations were obtained by performing Fourier Transform and were higher than correlations in time domain.The spatial distributions indicated that the effects of SST and PAR on Chl a were greater than AOT and WS in the Bohai Sea.Additionally,cross-spectrum analysis was applied to explore the response relationships.A depth-dependent pattern was shown in correlations and time lags,indicating that the influential mechanism of environmental factors on Chl-a concentration is related to seawater depth.

Ethylene accelerates maize leaf senescence in response to nitrogen deficiency by regulating chlorophyll metabolism and autophagy

Leaf senescence is an orderly and highly coordinated process,and finely regulated by ethylene and nitrogen(N),ultimately affecting grain yield and nitrogen-use efficiency(NUE).However,the underlying regulatory mechanisms on the crosstalk between ethylene-and N-regulated leaf senescence remain a mystery in maize.In this study,ethylene biosynthesis gene ZmACS7 overexpressing(OE-ZmACS7)plants were used to study the role of ethylene regulating leaf senescence in response to N deficiency,and they exhibited the premature leaf senescence accompanied by increased ethylene release,decreased chlorophyll content and F_v/F_m ratio,and accelerated chloroplast degradation.Then,we investigated the dynamics changes of transcriptome reprogramming underlying ethylene-accelerated leaf senescence in response to N deficiency.The differentially expressed genes(DEGs)involved in chlorophyll biosynthesis were significantly down-regulated,while DEGs involved in chlorophyll degradation and autophagy processes were significantly up-regulated,especially in OE-ZmACS7 plants in response to N deficiency.A gene regulatory network(GRN)was predicted during ethylene-accelerated leaf senescence in response to N deficiency.Three transcription factors(TFs)ZmHSF4,Zmb HLH106,and ZmEREB147 were identified as the key regulatory genes,which targeted chlorophyll biosynthesis gene ZmLES22,chlorophyll degradation gene ZmNYC1,and autophagy-related gene ZmATG5,respectively.Furthermore,ethylene signaling key genes might be located upstream of these TFs,generating the signaling cascade networks during ethylene-accelerated leaf senescence in response to N deficiency.Collectively,these findings improve our molecular knowledge of ethylene-accelerated maize leaf senescence in response to N deficiency,which is promising to improve NUE by manipulating the progress of leaf senescence in maize.

Degree of shade tolerance shapes seasonality of chlorophyll, nitrogen and phosphorus levels of trees and herbs in a temperate deciduous forest

Forest productivity is closely linked to seasonal variations and vertical differentiation in leaf traits.However,leaf structural and chemical traits variation among co-existing species,and plant functional types within the canopy are poorly quantified.In this study,the seasonality of leaf chlorophyll,nitrogen(N),and phosphorus(P)were quantified vertically along the canopy of four major tree species and two types of herbs in a temperate deciduous forest.The role of shade tolerance in shaping the seasonal variation and vertical differentiation was examined.During the entire season,chlorophyll content showed a distinct asymmetric unimodal pattern for all species,with greater chlorophyll levels in autumn than in spring,and the timing of peak chlorophyll per leaf area gradually decreased as shade tolerance increased.Chlorophyll a:b ratios gradually decreased with increasing shade tolerance.Leaf N and P contents sharply declined during leaf expansion,remained steady in the mature stage and decreased again during leaf senescence.Over the seasons,the lower canopy layer had significantly higher chlorophyll per leaf mass but not chlorophyll per leaf area than the upper canopy layer regardless of degree of shade tolerance.However,N and P per leaf area of intermediate shade-tolerant and fully shade-tolerant tree species were significantly higher in the upper canopy than in the lower.Seasonal variations in N:P ratios suggest changes in N or P limitation.These findings indicate that shade tolerance is a key feature shaping inter-specific differences in leaf chlorophyll,N,and P contents as well as their seasonality in temperate deciduous forests,which have significant implications for modeling leaf photosynthesis and ecosystem production.

Antioxidase System of a Rice Mutant with Low Chlorophyll b

[Objective] The mitigative effect of antioxidase system of a rice mutant with low chlorophyll b on photooxidative damage was studied.[Method] A rice mutant with low chlorophyll b and its wild type were taken as experimental materials to comparatively research their peroxide (H2O2) contents, the activity and isozymes of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in chloroplast.[Result] Compared with the wild type, there were many kinds of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant, and the activity of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant was also correspondingly higher. Under intense light condition, the H2O2 content of chloroplast in mutant was less than that in the wild type. [Conclusion] The higher activity of scavenging active oxygen can relieve the photooxidative damage made by excessive light energy of intense light on photosynthetic membrane, which is an important reason for higher photosystem Ⅱ (PS II) stability of this mutant.

Study on a Mutant with Low Content Chlorophyll b in a High Yielding Rice and Its Photosynthesis Properties

A high yielding rice mutant ( Oryza sativa L. cv. Zhenhui 249) with low chlorophyll b was recently discovered in the field. The mutant was mainly characterized by the decrease of the content of extrinsic antennae complex. This variation was shown in the stage when the leaves were expanding. When the leaves are at the final developmental stage, the content would approach to that of the wild type. It was discovered that only moderate amount of chlorophyll b decreased in this mutant. The photosynthetic apparatus of the mutant was rather stable in the whole life span of the leaf. The extrinsic antennae complex of the mutant might make efficient use of light and meanwhile reduce the production of O -· 2.

基于无人机高光谱遥感的水体CHL-a反演

无人机平台搭载高光谱传感器获取的高光谱数据具有高分辨率、波谱丰富的特点,本研究基于实测采样点光谱特征、采样点CHL-a浓度,采用相关性分析的方法选取最优波段,构建水体CHL-a反演模型,应用于无人机高光谱数据进行CHL-a的反演监测。结果表明:通过分析单波段、一阶微分值与CHL-a参数,发现使用674 nm、570nm波长构建的一阶微分与水体CHL-a构建的线性反演模型,具有较高的相关性与精度,可满足对小中型地表水体叶绿素的快速反演监测。基于反演结果构建空间分布图,实现水质参数的可视化。

Correlation Analysis of SPAD Value with Chlorophyll Content and Economic Yield Traits of Brassica napus L.

[Objective] The aim was to compare differences of SPAD value, chloro- phyll content, agronomic characters, economic characters and yield traits to analyze correlation of SPAD value with other indices and establish regression functions. [Method] Based on 34 Brassica napus L. varieties, SPAD value, chlorophyll content, agronomic characters, economic characters and yield traits were measured and re- gression functions were established according to correlations. [Result] SPAD value, chlorophyll content, agronomic and economic characters and yield traits all achieved significant level in differences among 34 varieties. Specifically, SPAD value was of extremely significant correlation with chlorophyll a and b, total chlorophyll and carotenoid, and the correlation from high to low was chl-b〉chl-z〉chl-a〉chl-x. SPAD value was of significantly positive correlation with total pod number per plant, plant height, seed number per pod, yield per plant and harvest yield, and of insignificant correlation with branch point height, effective branch number, pod density of main stem, and pod length. [Conclusion] It is simple and rapid to predict chlorophyll con- tent, economic characters and yields of Brassica napus L. with SPAD value and re- gression functions.

Grey Relational Analysis between Chlorophyll a and Environmental Factors in ShaHu Lake

[Objective] The aim was to explore effects of environmental factors on the content of Chlorophyll a in ShaHu Lake.[Method] Based on the data in Shahu Lake from November in 2007 to September in 2008,the relationship between chlorophyll a and environmental factors like water temperature,pH,secchi-depth （SD）,total nitrogen,total phosphorus and potassium permanganate index was studied by grey relational analysis method.[Result] The main environmental factors affecting the content of Chlorophyll a in ShaHu Lake were in order of water temperature potassium permanganate index 〉total nitrogen 〉pH〉 total phosphorus 〉SD.[Conclusion] The research provides reference for the control of eutrophication and the reasonable development and utilization of Shahu Lake.

Characteristics of Chlorophyll Fluorescence and Membrane-lipid Peroxidation of Various High-yield Rices Under Photooxidation Conditions

Using various high-yield rices (Oryza sativa L.) such as japonica cultivar 9516, two parental line hybrid rice between subspecies with more japonica element Peiai 64/E32, Liangyoupeijiu (Peiai 64/9311), and indica hybrid rices X07S/Zihui 100, Gangyou 881, Shanyou 63 as the materials, the characteristics of chlorophyll fluorescence and membrane-lipid peroxidation of detached leaves at booting stage under photooxidation conditions were studied. In comparison with indica hybrid rice, after the photooxidation treatment, the primary photochemical efficiency of PS II (F-v/F-m), quantum yield of linear electron transport of PS II (Phi(PSII)) and photochemical quenching coefficient (qP) in japonica cultivar and hybrid rice with japonica decreased less. This indicated that high-yield rice with japonica was able to maintain higher capability of light energy conversion, resulting in the alleviation of photoinhibition. Meanwhile, the higher activities of protective enzymes such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) led to the less accumulation of endogenous active oxygen (O-(2)(radical anion), H2O2) and less content of the malondialdehyde (MDA) and the less decline of chlorophyll and protein contents, indicating a stronger tolerance to photooxidation. The changes in contents of chlorophyll and protein among various nee cultivars during photooxidation treatment were consistent with the decline of chlorophyll content from heading stage to maturation stage under natural conditions. Statistical analysis showed that there was a significant correlation between the indexes of tolerance to photooxidation and the rate of seed setting, implying that the cultivar tolerated to photooxidation had higher resistance to early aging of leaf. These results suggested that from a view of superhigh-yield breeding, considering both the utilization of heterosis and the resistance to early aging of leaf, introduction of japonica element tolerating to photooxidation into the rice sterile line (maternal plant) is a breeding strategy worthy to pay great attention to.

Characteristics of CO_2 Exchange and Chlorophyll Fluorescence of Transgenic Rice with C_4 Genes

The responses of photosynthesis of phosphoenopyruvate carboxylase (PEPC), pyrurate dikinase (PPDK), NADP-malic enzyme (NADP-ME) and PPDK+PEPC transgenic rice (Oryza saltiva L.) plant to light, temperature, CO 2 and the characteristics of chlorophyll fluorescence under photoinhibition conditions were studied. The results were as follows: 1. The light-saturated photosynthetic rates of transgenic rice plants were higher than that of wild type, in which the light-saturated point of PEPC and PPDK+PEPC transgenic rice plants was 200 μmol·m -2·s -1 higher than that of untransformed rice and the light-saturated photosynthetic rates were 51.6% and 58.5% respectively. The carboxylation efficiency of PEPC transgenic rice plant increased by 49.3% and the CO 2 compensation point decreased by 26.2% than that of untransformed rice. Under high temperature (35 ℃), the photosynthetic rate of PEPC transgenic rice plant was higher over 17.5% than that of untransformed rice. 2. On the 8th day after photoinhibition treatment, the PSⅡ photochemical efficiency (F v/F m) and photochemical quenching (qP) of PEPC and PPDK+PEPC transgenic rice plants decreased by about 20%-30% while the non-photochemical quenching (qN) increased by approximately 30%. But F v/F m and qP of untransformed rice decreased by over 50% while qN increased by less than 10%. The result suggested that transgenic rice plants were more tolerant to photoinhibition.

A REMOTE SENSING MODEL FOR DETERMINING CHLOROPHYLL CONTENT AND ITS DISTRIBUTION USING LANDSAT IMAGES

The uncertainty in the estimatation of chlorophyll content with the use of normalized difference vegetation index (NDVI) has been described. To determine the chlorophyll content, model 1 for LANDSAT and model 2 for NOAA AVHRR wavebands were presented and have been verified by field experiments. Model 1 was also validated by the distribution of chlorophyll content using LANDSAT images around the Yucheng remote sensing experimental station. Using these models to estimate the chlorophyll content in the vegetation community is benefitiated by the increased precision and decreased uncertainty.

Three-stage Transformation of Chlorophyll Transient Fluorescence Pattern Under Sustained Dehydration and the Discovery of Critical Water Content in Seaweeds

The chlorophyll fluorescence kinetics of marine red alga Grateloupia turutunt Yamada, green alga Ulva pertusa Kjellm and brown alga Laminaria japonica Aresch during natural sustained dehydration were monitored and investigated. The pulse amplified modulation (PAM) system was used to analyze the distinct fluorescence parameters during thallus dehydration. Results proved that the fluorescence kinetics of different seaweed all showed three patterns of transformation with sustained water loss. These were: 1) peak kinetic pattern (at the early stage of dehydration fluorescence enhanced and quenched subsequently, representing a normal physiological state). 2) plateau kinetic pattern (with sustained water loss fluorescence enhanced continuously but quenching became slower, finally reaching its maximum). 3) Platform kinetic pattern (fluorescence fell and the shape of kinetic curve was similar to plateau kinetic pattern). A critical water content (CWC) could be found and defined as the percentage of water content just prior to the fluorescence drop and to be a significant physiological index for evaluation of plant drought tolerance. Once thallus water content became lower than this value the normal peak pattern can not be recovered even through rehydration, indicating an irreversible damage to the thylakoid membrane. The CWC value corresponding to different marine species were varied and negatively correlated with their desiccation tolerance, for example. Laminaria japonica had the highest CWC value (around 90%) and the lowest dehydration tolerance of the three. In addition, a fluorescence 'burst' was found only in red algae during rehydration. The different fluorescence parameters F-o, F-v and F-v, F-m were measured and compared during water loss. Both F-o and F-v increased in the first stage of dehydration but F-v/F-m. kept almost constant. So the immediate response of in vivo chlorophyll fluorescence to dehydration was an enhancement. Later with sustained dehydration F-o increased continuously while F-v decreased and tended to become smaller and smaller. The major changes in fluorescence (including fluorescence drop during dehydration and the burst during rehydration) were all attributed to the change in F-o instead of F-v This significance of F-o indicates that it is necessary to do more research on F-o as well as on its relationship with the state of thylakoid membrane.

Effects of Salicylic Acid on Photosynthesis and Chlorophyll Fluorescence Characteristics of Fluecured Tobacco Leaf in Subdued Light

[Objective] This study aimed to test whether salicylic acid （SA） can im-prove the physiological functions of flue-cured tobacco under subdued light condition, and to determine the mechanism of its action. [Method] The tobacco plants under subdued light were foliar-sprayed with 100 mg/L of SA. Then, the physiological in-dices such as plant fresh weight and dry weight, chlorophyl content, photosynthetic parameters and chlorophyl fluorescence parameters were measured. SPSS17.0 and Excellwere adopted for variance analysis and significance test. [Result] The leaf photosynthetic rate （Pn）, stomatal conductance （Gs） and transpiration rate （Tr） of tobacco plants in subdued light were al decreased while the intercellular CO2 con-centration （Ci） was increased, suggesting that non-stomatal limitation led to the de-crease of Pn under weak light intensity stress. SA released the inhibition of tobacco plant growth in weak light, as it elevated the leaf photosynthetic rate, the maximum photochemical efficiency of PSⅡ, potential activity of PSⅡ, effective photochemical efficiency of PSⅡ and photochemical quenching coefficient in weak light significant-ly, and reduced the non-photochemical quenching coefficient. [Conclusion] SA has significant effects on the photosynthetic characteristics of flue-cured tobacco in weak light, and it can improve the synthesis or distribution of photosynthesis product, and the efficiency of light energy, conducive to plant growth and development.

Heterogeneity of Chlorophyll Fluorescence of Phyllostachys edulis cv.Pachyloen Leaves

[Objective] This study aimed to explore the heterogeneity of chlorophyll flu- orescence of Phyllostachys edulis cv. Pachyloen leaves. [Method] Using the chloro- phyll fluorescence system and fluorescence imaging system in the Mini-IMAGING- PAM, the chlorophyll fluorescence of Phyllostachys edulis cv. Pachyloen leaves were measured and the fluorescence parameters were calculated. [Result] The homo- geneities of the maximal quantum yield of PS II （Fv/Fm） and the leaf absorptivity （Abs） were higher, with CVs （Coefficient of Variation） of 1.58%-1.68% and 1.75%- 2.12% respectively, while the heterogeneities of the actual quantum yield （Y）, non- photochemical quenching （NPQ/4）, photochemical quenching（qP） and relative photo- synthetic rate （PS/50） were higher, with CVs of 9.60%-14.23%, 10.23%-13.02%, 11.92%-13.02% and 11.15%-17.74% respectively. The trends of heterogeneity change in Y, qP and PSI50 were basically the same with transversely higher het- erogeneity at two sides and lower heterogeneity in the middle, namely with larger CVs at the edges of leaves and smaller ones around the midrib. Longitudinally, the coefficients of variation of Y, qP and PS/50 decreased gradually （from top to bot- tom）, which indicated that the heterogeneity declined from the leaf tip to the base. The trends of heterogeneity change in PS/50 and NPQ/4 were opposite. [Conclusion] The fluorescence parameters of Phyllostachys edulis cv. Pachyloen leaves revealed different heterogeneity.

Variable chlorophyll fluorescence in response to water plus heat stress treatments in three coniferous tree seedlings

Effects of water and heat stress treatments on chlorophyll fluorescence of Chinese fir (Cunninghamia lanceolata), Masson pine (Pinus massoniana) and western redcedar (Thuja plicata D. Don)_seedlings were monitored during a three-cycle stress period. It was shown that ratio of variable to maximal chlorophyll fluorescence (Fv/Fm) of these three species responded differently to water stress treatments. The Fv/Fm ratio of western redcedar decreased dramatically after water stress, while that of Chinese fir had only a slight reduction and that of Masson pine had no significant change. The experiment also showed that the Fv/Fm ratio of all three species differed significantly under heat stress treatments. Concerning three different water plus heat stress cycles, it was found that the Fv/Fm ratios of Chinese fir and Masson pine measured at the end of each water plus heat stress cycle were not significantly different. However, the Fv/Fm ratio of western redcedar was diminished significantly in response to an increase of stress time. Keywords Chinese fir - Chlorophyll fluorescence - Heat stress - Masson pine - Water stress - Western redcedar CLC number Q945.17 - S791.248 Document code A Biography: Yu Fang-yuan (1965-), male, Ph. Doctor. Associate professor in College of Forest Resources and Environment, Nanjing, Forestry University, Nanjing 210037, P. R. China.Responsible editor: Zhu Hong

Effects of NaCl Stress on Chlorophyll Fluorescence Parameters in Cinnamomum japonicum var. chenii

[ Objective ] Study on the changes of chlorophyll fluorescence parameters in Cinnamomumjaponicum var. chenii under NaCl stress. [ Method ] The seedling growth increment, chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [ Result] Under NaC1 stress, seedling growth increment reduced and the chlorophyll content decreased to a stable value ; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress, most variations were observed in Fo, correlations among chlorophyll fluorescence parameters presented ＂rise-drop＂ trend （in.the treatment of 7 g/L NaCl）. [ Conclusion] Cirmamomum japonicum vat. chenii is endowed with strong salt resistance and wide adaptability.

The Influence of Surfactants on Chlorophyll Binding Status and Excitation Energy Transfer in Photosystem Ⅰ of Wheat

Surfactants are widely used in the purification and research of structure and function of the protein complexes in photosynthetic membrane. To elucidate the mechanism of interaction between surfactants and photosystem Ⅰ (PSⅠ), effects of two typical surfactants, Triton X_100 and sodium dodecyl sulfate (SDS) on PSⅠ, were studied at different concentrations. The results were: SDS led to the reduction of apparent absorption intensity and blue shift of absorption peaks; while Triton X_100 led to the decrease of apparent absorption intensity in red region and blue shift of the peak, but to the increase of apparent absorption intensity in blue region. The fourth derivative spectra show that the longwavelength (669 nm and 683 nm) absorbing chlorophyll a was affected greatly and their relative changes of absorbance were axially symmetrical. The presence of surfactant could make the long wavelength fluorescence emission decrease greatly and a new fluorescence peak appeared around 680 nm, it was obvious that the surfactant interceded the transfer of excitation energy from antenna pigments to reaction center. The surfactants might affect the microenvironment of proteins, even the structure of PSⅠ protein subunits and hence changed the binding status of pigments with protein subunits, or the pigments might be released from the subunits. All of these might affect the absorption and the transfer of excitation energy.

Response of Ficus microcarpa L.Foliage to Water Stress Determined by Chlorophyll Fluorescence Imaging Technique

[Objective] This study was to determine the response of Ficus microcarpa L. foliage to polyethylene glycol （PEG） simulated water stress using chlorophyll fluo- rescence imaging technique. [Method] The responses of detached leaves from Ficus microcarpa, Ficus benjamina and Nerium oleander to PEG-6000 simulated water stress were detected, and the chlorophyll fluorescence imaging technique was used to detect and analyze the stress at different spots of a single leaf simultaneously. [Result] The responses of Ficus microcarpa, Ficus benjamina and Nerium oleander to dehydration showed that： ~1~） the maximal photochemical efficiency （Fv/Fm） and non- photo-chemical quenching （NPQ） values were small in the reaction center among different detected spots of leaves, and there were great differences between relative electron transport rate （ETR）, photochemical quenching （qP） and quantum efficiency of PSII photochemistry （（φPSII）; （2） the differences of these parameters were more ob- vious among different spots of water-stressed leaves; （3） the discrete degrees of the species with strong resitances decreased significantly. [Conclusion] This study lays the foundation for the further research on the response of plants to drought stress using chlorophyll fluorescence imaging technique.

Dynamic Change Laws of Chlorophyll Fluorescence Parameters in Different Parts of Leaves of Ginkgo biloba

[Objective] The aim was to explore the dynamic change laws of chlorophyll fluorescence parameters in different parts of leaves of Ginkgo biloba.[Method] The G.biloba cultivated in North China was used as materials in this study to explore the law of daily change and ten-day change of the chlorophyll fluorescence parameters of leaves in different parts of leaves.[Result] The daily change of Fm（maximal fluorescence）,Fv（variable fluorescence）,Fv/Fm,Fm/Fo（electron transfer rate）,Fv/Fo（potential activity of PSⅡ）in leaves of G.biloba obviously presented a descending-ascending trend,the lowest value was at 12：00 and the NPQ（non-photochemical quenching）of sunny leaves arrived at the maximum at noon.The values of Fm,Fv,Fv/Fm,Fm/Fo,Fv/Fo in shade leaves of G.biloba were obviously higher than those in sunny leaves,but the peak value of NPQ of shade leaves presented earlier and higher,suggesting that the shade leaves might have more sensitive hot dissipation mechanism.Comparing to sunny leaves,shade leaves had the higher PSⅡ potential activity and inner light energy translation efficiency.[Conclusion] This study had provided theoretical basis for the protection of G.biloba resources.