期刊文献+
共找到2,435篇文章
< 1 2 122 >
每页显示 20 50 100
Phylogenetic study on Scenedesmacae with the description of a new genus Coccoidesmus gen.nov.(Chlorophyceae,Chlorophyta)and chloroplast genome analyses
1
作者 Qinghua WANG Ying HOU +2 位作者 Yanhui LI Ying SHI Guoxiang LIU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第4期1272-1285,共14页
Members of the family Scenedesmaceae are some of the most common algal taxa in inland ecosystems,and they are widely distributed in freshwaters,aerial,and sub-aerial habitats.With the continuous updating of methods,th... Members of the family Scenedesmaceae are some of the most common algal taxa in inland ecosystems,and they are widely distributed in freshwaters,aerial,and sub-aerial habitats.With the continuous updating of methods,the classic morphological taxonomy of this family needs to be revised.In recent years,many genera of Scenedesmaceae have been established via the use of molecular methods.The phylogenetic relationships within Scenedesmaceae were analyzed using different molecular markers and morphological data,and the new freshwater genus Coccoidesmus Wang,Hou et Liu gen.nov.was described.Two new species in this genus were also described.Phylogenetic analysis based on tufA genes revealed that the new genus formed an independent clade closely related to Comasiella.However,these two genera are characterized by significant morphological differences in colony arrangement and cell shape.The chloroplast genome of the type species was assembled and annotated,and analyses of genome structure and sequences were conducted.More genome data could help clarify the phylogenetic relationships within this family. 展开更多
关键词 PHYLOGENETIC Scenedesmaceae Coccoidesmus morphology chloroplast genome
下载PDF
A Golden2-like transcription factor, BnGLK1a, improves chloroplast development, photosynthesis, and seed weight in rapeseed
2
作者 Qianwei zhang Yuanyi Mao +11 位作者 Zikun Zhao Xin Hu Ran Hu Nengwen Yin Xue Sun Fujun Sun Si Chen Yuxiang jiang Liezhao Liu Kun Lu Jiana Li Yu Pan 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第5期1481-1493,共13页
Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.In this study,we demonstrate that Golde... Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.In this study,we demonstrate that Golden2-like 1a(BnGLK1a)plays an important role in regulating chloroplast development and photosynthetic efficiency.Overexpressing BnGLK1a resulted in significant increases in chlorophyll content,the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus,while knocking down BnGLK1a transcript levels through RNA interference(RNAi)had the opposite effects.A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-LIKE 1-2(BnPYL1-2)and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit(BnCSN5A),which play essential roles in regulating chloroplast development and photosynthesis.Consistent with this,BnGLK1a-RNAi lines of B.napus display hypersensitivity to the abscisic acid(ABA)response.Importantly,overexpression of BnGLK1a resulted in a 10%increase in thousand-seed weight,whereas seeds from BnGLK1a-RNAi lines were 16%lighter than wild type.We propose that BnGLK1a could be a potential target in breeding for improving rapeseed productivity.Our results not only provide insights into the mechanisms of BnGLK1a function,but also offer a potential approach for improving the productivity of Brassica species. 展开更多
关键词 Brassica napus BnGLK1a chloroplast development photosynthetic efficiency YIELD
下载PDF
Comparative and Phylogenetic Analysis of the Complete Chloroplast Genomes of 19 Species in Rosaceae Family
3
作者 Riwa Mahai Rongpeng Liu +3 位作者 Xiaolang Du Zejing Mu Xiaoyun Wang Jun Yuan 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第6期1203-1219,共17页
Rosaceae represents a vast and complex group of species,with its classification being intricate and contentious.The taxonomic placement of many species within this family has been a subject of ongoing debate.The study ... Rosaceae represents a vast and complex group of species,with its classification being intricate and contentious.The taxonomic placement of many species within this family has been a subject of ongoing debate.The study utilized the Illumina platform to sequence 19 plant species from 10 genera in the Rosaceae.The cp genomes,vary-ing in size from 153,366 to 159,895 bp,followed the typical quadripartite organization consisting of a large single-copy(LSC)region(84,545 to 87,883 bp),a small single-copy(SSC)region(18,174 to 19,259 bp),and a pair of inverted repeat(IR)regions(25,310 to 26,396 bp).These genomes contained 132–138 annotated genes,including 87 to 93 protein-coding genes(PCGs),37 tRNA genes,and 8 rRNA genes using MISA software,52 to 121 simple sequence repeat(SSR)loci were identified.D.arbuscular contained the least of SSRs and did not have hexanotides,A.lineata contained the richest SSRs.Long terminal repeats(LTRs)were primarily composed of palindromic and forward repeat sequences,meanwhile,The richest LTRs were found in Argentina lineata.Except for Argentina lineata,Fragariastrum eriocarpum,and Prunus trichostoma,which varied in gene type and position on both sides of the boundary,the remaining species were found to be mostly conserved according to IR boundary analysis.The examination of the Ka/Ks ratio revealed that only the infA gene had a value greater than 1,indicating that this gene was primarily subjected to positive selection during evolution.Additionally,9 hotspots of variation were identified in the LSC and SSC regions.Phylogenetic analysis confirmed the scientific validity of the genus Prunus L.sensu lato(s.l.)within the Rosaceae family.The separation of the three genera Argentina Hill,Fragariastrum Heist.ex Fabr.and Dasiphora Raf.from Potentilla L.may be a more scientific classification.These results offer fresh perspectives on the taxonomy of the Rosaceae. 展开更多
关键词 ROSACEAE chloroplast genomes comparative genomes PHYLOGENY
下载PDF
Structural Characterization of Chloroplast Genome in Alpinia japonica(Thunb.)Miq.,a Medicinal Plant of the Genus Alpinia
4
作者 Wentao Sheng Xi Lei +1 位作者 Xinjie Chen Quan Kuang 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第8期1897-1911,共15页
The analysis of chloroplast gene characteristics in Alpinia japonica(Thunb.)Miq.is of great significance for developing relevant genetic resources.The high-throughput sequencing and bioinformatic research were perform... The analysis of chloroplast gene characteristics in Alpinia japonica(Thunb.)Miq.is of great significance for developing relevant genetic resources.The high-throughput sequencing and bioinformatic research were performed to analyze the chloroplast genome characteristics of A.japonica.The total chloroplast genome length of A.japonica was 161,906 bp,with a typical circular tetrameric structure.And 133 genes were annotated,comprising 87 protein-coding,38 tRNA,and 8 rRNA genes.Furthermore,22 genes contained two copies,and 18 genes owned introns.Repeat sequence analysis showed that it contains 321 simple sequence repeats(SSRs)and 37 long segment repeats.Compared with the chloroplast genomes of eight representative plants in the genus Alpinia,the gene structure,type,and quantity were relatively conservative.Rps12 was the highest variation site in the entire chloroplast gene.A phylogenetic tree showed that the genus Alpinia was the most closely related to the genus Amomum.Meanwhile,A.japonica is the most closely related to Alpinia chinensis belonging to the genus Alpinia.Overall,the chloroplast genome of a new species was reported in the genus Alpinia,and a basis was provided for the utilization of Alpinia plants as a medical resource. 展开更多
关键词 The genus Alpinia Alpinia japonica(Thunb.)Miq chloroplast genome phylogenetic tree
下载PDF
基于叶绿体atpB-rbcL序列探讨猬草属和赖草属植物的系统发育和母系起源 被引量:5
5
作者 刘静 张海琴 +3 位作者 凡星 沙莉娜 曾建 周永红 《草业学报》 CSCD 北大核心 2012年第5期77-85,共9页
对小麦族猬草属、赖草属(NsXm)、披碱草属(StH)及含不同染色体组(St、H、Ns、Eb、Ee、P、F、V、W)的近缘二倍体属共46个类群的叶绿体基因间隔区atpB-rbcL序列进行系统发育分析,探讨猬草属和赖草属植物系统发育关系及母系起源。结果显示... 对小麦族猬草属、赖草属(NsXm)、披碱草属(StH)及含不同染色体组(St、H、Ns、Eb、Ee、P、F、V、W)的近缘二倍体属共46个类群的叶绿体基因间隔区atpB-rbcL序列进行系统发育分析,探讨猬草属和赖草属植物系统发育关系及母系起源。结果显示,1)猬草属模式种H.patula与拟鹅观草属和披碱草属物种聚为一支,表明H.patula与披碱草属植物亲缘关系近,其母本来源为含St染色体组的拟鹅观草属物种;2)猬草、长芒猬草、4个新麦草属物种及滨麦和所有欧亚分布的赖草属物种聚在一支,说明猬草、长芒猬草与欧亚赖草亲缘关系较近,其母本供体来自含Ns染色体组的新麦草属物种;3)高丽猬草和东北猬草与北美赖草及冰草属、旱麦草属、大麦属物种处于同一分支,表明高丽猬草和东北猬草与北美赖草具有较近亲缘关系,其母本供体为未知来源的Xm染色体组。研究结果支持将H.patula组合到披碱草属中,猬草、长芒猬草、高丽猬草和东北猬草应组合到赖草属中。 展开更多
关键词 猬草属 赖草属 atpb-rbcl 系统发育 母系起源 染色体组
下载PDF
基于叶绿体DNA atpB-rbcL区序列探讨石蒜属种间系统发育关系 被引量:2
6
作者 魏磊 庞磊 +1 位作者 杨旭 史树德 《热带亚热带植物学报》 CAS CSCD 北大核心 2013年第2期109-115,共7页
为了探讨石蒜属(Lycoris Herb.)植物的种间系统发育关系,对石蒜属植物95份材料,包括15种4变种及2个人工杂种的叶绿体DNAatpB-rbcL间隔区进行了测序,结合花部形态和核型特征,探讨了石蒜属种间系统关系及其可能的杂交起源。系统发育树的... 为了探讨石蒜属(Lycoris Herb.)植物的种间系统发育关系,对石蒜属植物95份材料,包括15种4变种及2个人工杂种的叶绿体DNAatpB-rbcL间隔区进行了测序,结合花部形态和核型特征,探讨了石蒜属种间系统关系及其可能的杂交起源。系统发育树的分析结果表明,矮小石蒜(L.radiata var.pumila)和换锦花(L.sprengeri)与2个人工杂交种、麦秆石蒜(L.straminea)、江苏石蒜(L.houdyshelii)、短蕊石蒜(L.caldwellii)和乳白石蒜(L.albiflora)具有密切的亲缘关系。atpB-rbcL序列揭示的石蒜属种间关系与染色体核型的分类结果部分一致,主要表现在具有近端部着丝粒(A)染色体的种与具有中部(M)和端部(T)着丝粒染色体的种各成一支,与形态和染色体分类结果一致;不同之处在于具有中部、端部和近端部着丝粒染色体的种分散在两个主要分支内,进一步验证了具有中部、端部和近端部3种着丝粒类型染色体组的石蒜,如麦秆石蒜、江苏石蒜、短蕊石蒜和乳白石蒜等,是杂交起源的假设,结合2个人工杂交种分析,揭示了短蕊石蒜和乳白石蒜的近端部着丝粒染色体来源于换锦花;麦秆石蒜和江苏石蒜近端部着丝粒染色体来源于矮小石蒜。 展开更多
关键词 石蒜属 叶绿体atpb-rbcl序列 种间系统发育关系 杂交起源 系统发育树
下载PDF
叶绿体atpB-rbcL序列分析在核果类果树系统发育研究中的应用 被引量:3
7
作者 王化坤 陶建敏 +4 位作者 渠慎春 马瑞娟 房经贵 娄晓鸣 章镇 《果树学报》 CAS CSCD 北大核心 2010年第2期213-220,共8页
为重建核果类果树的系统发育,选择桃、李、梅、杏、樱桃各4~5个主要种或变种,共24个基因型,测定了其叶绿体非编码区atpB-rbcL序列。以桂樱为外种群,用PAUP软件计算数据集的得分,Modeltest筛选最佳模型和参数,Mega计算遗传距离、变异,... 为重建核果类果树的系统发育,选择桃、李、梅、杏、樱桃各4~5个主要种或变种,共24个基因型,测定了其叶绿体非编码区atpB-rbcL序列。以桂樱为外种群,用PAUP软件计算数据集的得分,Modeltest筛选最佳模型和参数,Mega计算遗传距离、变异,最大简约法构建系统发育树。结果表明,1)atpB-rbcL在核果类果树各组间分子进化速率不同,差异公布不均衡;2)樱桃较其他核果类果树原始;李、梅、杏亲缘关系较近,梅、杏关系最近。核果类果树是一个单系群,由一个共同的祖先沿着2个方向进化,一枝进化为樱桃,另一枝沿不同的途径产生桃、李、梅、杏等核果类果树。 展开更多
关键词 核果类果树 系统发育 atpb-rbcl 分子进化
下载PDF
Effects of High K^+ and Alkaline pH on Ultrastructure of Dunaliella salina Chloroplasts 被引量:2
8
作者 马淑英 黄阳成 +1 位作者 杨小贺 武维华 《Acta Botanica Sinica》 CSCD 1999年第12期1342-1344,共3页
ItwasreportedthatthegrowthofDunaliellasalinaTeod .culturedinmediumcontaining 1mol/L NaClwasalmostcompletelyinhibitedbytheadditionof 10 0mmol/LKCl.ThehighK+ (10 0mmol/LKCl) treatmentalsosignificantlyinhibitedthephoto... ItwasreportedthatthegrowthofDunaliellasalinaTeod .culturedinmediumcontaining 1mol/L NaClwasalmostcompletelyinhibitedbytheadditionof 10 0mmol/LKCl.ThehighK+ (10 0mmol/LKCl) treatmentalsosignificantlyinhibitedthephotosyntheticrateofD .salinaanddecreasedchlorophyllcontentsin algae .ThisstudyfocusesonpossibleeffectsofhighK+ oralkalinepHontheultrastructuralchangeofchloro plastsinD .salina .AfterD .salinawasculturedinamediumcontaining 10 0mmol/LKClorinamedium withalkalinepHfor 8to 10days ,dramaticultrastructuralchangesoccurredinthechloroplastsincludingthy lakoidswelling ,volumeincreaseofchloroplast,andsignificantaccumulationofstarchgrainsinchloroplasts . Theresultsareconsistentwithourpreviousreportindicatingthattheultrastructuralchangesinchloroplastun derhighK+ oralkalinepHmayleadtoaninhibitoryeffectsonphotosynthesisandoverallgrowthofD .sali na . 展开更多
关键词 Dunaliellasalina POTASSIUM chloroplast Starchgrain Osmoticregulation
下载PDF
Expression of the Thylakoid Membrane Localized PPF1 in Transgenic Arabidopsis Affects Chloroplast Development 被引量:3
9
作者 徐云剑 王大勇 朱玉贤 《Acta Botanica Sinica》 CSCD 2002年第11期1314-1320,共7页
PPF1 is a vegetative growth related gene that encodes a putative membrane protein having high homology with Arabidopsis chloroplast thylakoid protein ALB3. Immunoelectron microscopic assay showed that PPF1 was mainly ... PPF1 is a vegetative growth related gene that encodes a putative membrane protein having high homology with Arabidopsis chloroplast thylakoid protein ALB3. Immunoelectron microscopic assay showed that PPF1 was mainly localized in the thylakold membrane and was highly expressed in well-developed chloroplasts of short day (SD) grown G2 pea while having a very low abundance in chloroplasts of long day (LD) grown plants two weeks after flowering. Comparison of the leaf senescence processes in transgenic Arabidopsis and wild type plants revealed that overexpression of PPF1 delayed leaf senescence, while the depression of its Arabidopsts homologue (ALB3) with PPF1 antisense mRNA accelerated leaf senescence obviously. Ultrastructural analysis of transgenic Arabidopsis plants showed that when PPF1 was overexpressed in Arabidopsis, the chloroplasts were bigger and had much more grana and stroma thylakoid membranes than those of wild type plants. On the contrary, when PPF1 was expressed in antisense orientation to reduce the level of PPF1 homologue in Arabidopsis, the transgenic plants had smaller chloroplasts With less grana. and poorly developed thylakoid membrane systems. These results suggested that the developmental status of chloroplasts was positively correlated with the level of PPF1 or its Arabidopsts homologue, ALB3. Our results suggested that PPF1 gene might regulate plant development by controlling chloroplast development. 展开更多
关键词 PPF1 IMMUNOLOCALIZATION chloroplast development SENESCENCE
下载PDF
cpSSR: a New Tool to Analyze Chloroplast Genome of Citrus Somatic Hybrids 被引量:8
10
作者 程运江 郭文武 邓秀新 《Acta Botanica Sinica》 CSCD 2003年第8期906-909,共4页
Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs ... Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs from pine (Pinus thunbergii Parl.), rice (Otyza sativa L.) and tobacco (Nicotiana tabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gel electrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was then analyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gel electrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzed Citrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. It was also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids in higher plants, compared with the conventional RFLP or CAPS analyses. 展开更多
关键词 Citrus somatic hybrids MICROSATELLITE simple sequence repeat (SSR) chloroplast genome
下载PDF
Inheritance of Chloroplast and Mitochondrial DNA in Chinese Fir (Cunninghamia lanceolata) 被引量:8
11
作者 奇文清 杨慧君 +1 位作者 薛勇彪 胡适宜 《Acta Botanica Sinica》 CSCD 1999年第7期695-699,共5页
The inheritance of mitochondrial (mt) DNA and chloroplast (cp) DNA was investigated in intergeneric hybrids from crossing between Cunninghamia lanceolata (Lamb.) Hook. and Cryptomeria fortunei Hooibrenk. The c... The inheritance of mitochondrial (mt) DNA and chloroplast (cp) DNA was investigated in intergeneric hybrids from crossing between Cunninghamia lanceolata (Lamb.) Hook. and Cryptomeria fortunei Hooibrenk. The chloroplast trnL trnF region and one intra genic segment of the mitochondrial gene, Cox Ⅲ, were amplified from those of the parents and hybrids by PCR using gene specific primers. Cp and mtDNA polymorphisms of the amplified regions were detected between the parents after restriction digestions. Restriction fragment length polymorphism (RFLP) analysis revealed that all the F 1 individuals possessed Cox Ⅲ restriction fragment patterns (characteristic of the paternal parent Cryptomeria fortunei ) and the trnL trnF region (identical to the maternal parent Cunninghamia lanceolata ) showing that a different mode of inheritance for organelle DNA has occurred in the hybrids. Furthermore, the maternal inheritance of chloroplast DNA is reported here for the first time in coniferophyta. 展开更多
关键词 Cunninghamia lanceolata Cryptomeria fortunei Inheritance of chloroplast DNA Inheritance of mitochondrial DNA Restriction fragment length polymorphism
下载PDF
Genetic Diversity of Indocalamus Determined by Chloroplast DNA Sequence 被引量:1
12
作者 牟少华 郄光发 彭镇华 《Agricultural Science & Technology》 CAS 2010年第4期50-52,89,共4页
[Objective]The aim was to research the relationship and genetic diversity of Indocalamus.[Method]Using 13 samples of Indocalamus and 3 samples of Sasa as materials,the intergenic regions of trnL-trnF gene in chloropla... [Objective]The aim was to research the relationship and genetic diversity of Indocalamus.[Method]Using 13 samples of Indocalamus and 3 samples of Sasa as materials,the intergenic regions of trnL-trnF gene in chloroplast were amplified by PCR,and sequence analysis and phylogenetic trees construction were carried out.[Result]Using the universal primer,the intergenic regions of trnL-trnF were amplified,the lengths of the segments varied from 1 008 bp to 1 103 bp,of which 940 bp was compared.The dendrogram of trnL-trnF sequences showed that Indocalamus and Sasa were clustered together and they were homologous by 99%.All the samples were divided into five groups,the first group included 12 samples such as Indosalamus pedalis,I.pumilus,I.victorialis,I.longiauritus,I.tessellatus,Sasa sinica,Sasa pygmaea,I.barbatus,I.guangdongensis,I.herklotsii,I.Hirtivaginatus and S.fortunei.I.decorus,I.lacunosus,I.Latifolius and I.Migoi were respectively divided into four groups.[Conclusion]The high homology of all samples showed the low evolution speed and little information sites which suggested that the phylogeny of Indocalamus could not be well resolved by the intergenic region of trnL-trnF. 展开更多
关键词 INDOCALAMUS chloroplast DNA SEQUENCE DIVERSITY
下载PDF
MECHANISM OF CHANGE IN THE CATION INDUCED EXCITA TION ENERGY DISTRIBUTION BETWEEN PSⅡ AND PSⅠ IN THE CHLOROPLAST MEMBRANE FROM ZOSTERA MARINA * 被引量:1
13
作者 高振泮 娄清香 +4 位作者 马红 翟小京 马桂芝 毛大璋 李良璧 《Acta Botanica Sinica》 CSCD 1995年第11期833-841,共9页
The interrelations between thylakoid polypeptide components and Mg 2+ induced Chl a fluorescence and thylakoid surface charge changes were investigated in Zostera marina chloroplasts treated with Ca 2+ and... The interrelations between thylakoid polypeptide components and Mg 2+ induced Chl a fluorescence and thylakoid surface charge changes were investigated in Zostera marina chloroplasts treated with Ca 2+ and trypsin. It was observed that:1. The increase of Mg 2+ induced PSⅡ fluorescence intensity was closely related to the decrease of Mg 2+ induced surface charge density of the thylakoid membrane in the normal chloroplast; 2. Removal of the 32~34 kD polypeptides of the thylakoid surface by Ca 2+ extraction of the chloroplast did not affect the Mg 2+ induced phenomena; 3. If the Ca 2+ treated chloroplast was further digested by trypsin to remove the 26 kD polypeptide of the membrane surface, the Mg 2+ induced phenomena disappeared completely. These results clearly indicated that the 26 kD polypeptide of thylakoid surface is the specific acting site of the cation that induced these two correlated phenomena in the chloroplast from Zostera marina. The mechanism on the regulating effect of the cation on excitation energy distribution between PSⅡ and PSⅠ was discussed. 展开更多
关键词 chloroplast PHOTOSYSTEM Excitation energy distribution
下载PDF
Chloroplast Genetic Engineering in Higher Plants 被引量:1
14
作者 张景昱 张嫄 宋艳茹 《Acta Botanica Sinica》 CSCD 2003年第5期509-516,共8页
Chloroplast genetic engineering, with several advantages over nuclear genetic engineering, is now regarded as an attractive new technology in basic and applied research, including deepening our understanding of plasti... Chloroplast genetic engineering, with several advantages over nuclear genetic engineering, is now regarded as an attractive new technology in basic and applied research, including deepening our understanding of plastid genome, engineering plant metabolic system, generating transplastomic plants with higher resistance to insect, disease, drought and herbicide and bioproducing of antibodies and vaccines. In this review, the principle and operating system for chloroplast genetic engineering and its application in higher plants have been discussed. 展开更多
关键词 chloroplast genetic engineering transplastomic plants plastid genome
下载PDF
Changes of Chorophyll protein Complexes and Photosynthetic Activities of Chloroplasts from Lotus (Nelumbo nucifera) Seeds Germinating in Light 被引量:1
15
作者 唐崇钦 左宝玉 +5 位作者 李国清 张泉 姜桂珍 冯丽洁 彭德川 匡廷云 《Acta Botanica Sinica》 CSCD 1999年第6期608-612,共5页
The changes of chlorophyll_protein complexes and photosynthetic activities of chloroplast isolated from lotus ( Nelumbo nucifera Gaertn.) seeds germinating under illumination were studied. SDS PAGE analysis of c... The changes of chlorophyll_protein complexes and photosynthetic activities of chloroplast isolated from lotus ( Nelumbo nucifera Gaertn.) seeds germinating under illumination were studied. SDS PAGE analysis of chlorophyll_protein complexes showed that there was only the light harvesting chlorophyll a/b protein complex from PSⅡ (LHCⅡ) precursor in chloroplast from lotus seeds germinated for 2 to 6 days, while LHC Ⅱ 1, and the chlorophyll_protein complex of PSⅠ (CPⅠ) appeared on the 8th day of germination and PSⅡ reaction center complex appeared later. Studies on the polypeptides composition of the chloroplast revealed the following results: 1) Small amount of the 27 kD polypeptide was synthesized in invisible light; 2) The 30 kD polypeptide existed previously in the plumules of the dry seeds; 3) The amount of the 30 kD polypeptide was more than any other polypeptides before germination and decreased gradually throughout germination, while the 27 kD polypeptide changed in the opposite way; 4) In the process of germination, measurement of the electron transport rate and the fluorescence induction kinetics at room temperature showed that PSⅡ activities and efficiency of primary light energy transformation were only experimentally measurable after 7 days of germination and gradually increased afterwards. At the same time, the chl a/b ratio rose from the lower value to normal; 5) The changes of chloroplast membrane components and its functions are concomitant in concert with that of the ultrastructure of chloroplast membranes during germination, as shown in our earlier work . The results have proved again that a different developmental pathway of chloroplast is likely to exist in the lotus plumules, which might provide an important clue for N. nucifera in having an unique position in the phylogeny of the angiosperm. 展开更多
关键词 LOTUS Plumule germination chloroplast development Chlorophyll protein complex POLYPEPTIDE Electron transfer rate
下载PDF
Construction of Double Cross-over Expression Vector for Chloroplast Multicistron in Brassica napus L. 被引量:1
16
作者 武玉永 姚庆收 马立新 《Agricultural Science & Technology》 CAS 2013年第3期402-406,共5页
[Objective] This study aimed to construct Brassica napus chloroplast multi- cistron double cross-over expression vector, to lay the foundation for the genetic engi- neering research of Brassica napus chloroplast. [Met... [Objective] This study aimed to construct Brassica napus chloroplast multi- cistron double cross-over expression vector, to lay the foundation for the genetic engi- neering research of Brassica napus chloroplast. [Method] Two primers were designed based on the known Brassica napus chloroplast DNA sequences AF267640 and Z50868 in GenBank. By using PCR method, two Brassica napus L. chloroplast DNA fragments were obtained, which were named RbcL and ACCD. The two Brassica na- pus chloroplast DNA homologous fragments were then cloned into plasmid pMD18-T to obtain recombinant plasmid pHBM715. Tandem expression cassette harboring spectinomycin-resistant gene aadA, mannanase gene man and green fluorescent pro- tein gene gfp was cloned into the plasmid pHBM715, thereby constructing Brassica napus chloroplast multicistron double cross-over expression vector pHBM716, which was transformed into Escherichia coil for expression and identification. [Result] Plate qualitative analysis was conducted for the functional identification of expression cas- sette in the constructed Brassica napus chloroplast multicistron double cross-over ex- pression vector, results showed that the three genes of the same multicistron were all expressed in E. coil [Conclusion] This study successfully constructed Brassica napus chloroplast multicistron double cross-over expression vector, which laid the foundation for the genetic engineering of Brassica napus chloroplast. 展开更多
关键词 Brassica napus L. chloroplast DNA Multicistron Double cross-over Expression vector Functional identification
下载PDF
桫椤atpB-rbcL序列2种测序方法的比较分析 被引量:2
17
作者 李媛 侯可雷 +2 位作者 张静 赵涛 刘志兵 《安徽农业科学》 CAS 北大核心 2009年第20期9405-9406,共2页
[目的]比较2种方法进行桫椤atpB-rbcL序列测定的测序效果。[方法]通过PCR产物直接测序和克隆测序对桫椤叶绿体atpB-rbcL基因间隔序列进行测定与分析。[结果]结果表明,桫椤atpB-rbcL序列直接测序所得峰形紊乱,PolyT后出现多重峰;克隆测... [目的]比较2种方法进行桫椤atpB-rbcL序列测定的测序效果。[方法]通过PCR产物直接测序和克隆测序对桫椤叶绿体atpB-rbcL基因间隔序列进行测定与分析。[结果]结果表明,桫椤atpB-rbcL序列直接测序所得峰形紊乱,PolyT后出现多重峰;克隆测序结果峰形清晰,无干扰。[结论]对于桫椤atpB-rbcL序列测定,克隆测序优于直接测序。 展开更多
关键词 桫椤 PCR产物直接测序 PCR产物克隆测序 atpb-rbcl非编码区
下载PDF
Effects of Doubled_CO_2 Concentration on Ultrastructure, Supramolecular Architecture and Spectral Characteristics of Chloroplasts from Wheat 被引量:1
18
作者 左宝玉 张泉 +2 位作者 姜桂珍 白克智 匡廷云 《Acta Botanica Sinica》 CSCD 2002年第8期908-912,共5页
Wheat ( Triticum aestivum L.) plants were grown under ambient and doubled_CO 2(plus 350 μL/L) concentration in cylindrical open_top chamber to examine their effects on the ultrastructure, supramolecular architect... Wheat ( Triticum aestivum L.) plants were grown under ambient and doubled_CO 2(plus 350 μL/L) concentration in cylindrical open_top chamber to examine their effects on the ultrastructure, supramolecular architecture, absorption spectrum and low temperature (77 K) fluorescence emission spectrum of the chloroplasts from wheat leaves. The results were briefly summarized as follows: (1) The wheat leaves possessed normally developed chloroplasts with intact grana and stroma thylakoid membranes; The grana intertwined with stroma thylakoid membranes and increased slightly in stacking degree and the width of granum, in spite of more accumulated starch grains within the chloroplasts than those in control; (2) The particle density in the stacked region of the endoplasmic fracture face (EFs) and protoplasmic fracture face (PFs) and in the unstacked region the endoplasmic fracture face (EFu) and the protoplasmic fracture face (PFu) was significantly higher than that of control. Furthermore, in some cases many more particles on EFs faces of thylakoid membranes appeared as a paracrystalline particle array; (3) The variations in the structure of chloroplasts were consistent with the absorption spectra and the low temperature (77 K) fluorescence emission spectra of the chloroplasts developed under the doubled_CO 2 concentration. Results indicate that the capability of light energy absorption of chloroplasts and regulative capability of excitation energy distribution between PSⅡ and PSⅠ were raised by doubled_CO 2 concentration. This is very favorable for final productivity of wheat. 展开更多
关键词 chloroplast wheat ( Triticum aestivum ) doubled_CO 2 concentration ultrastructure and supramolecular architecture absorption spectra low temperature (77 K) fluorescence emission spectra
下载PDF
基于ITS、atpB-rbcL序列的醉翁榆(Ulmus gaussenii)与相近榆属植物的亲缘关系
19
作者 韩雪 智颖飙 +5 位作者 张保卫 周忠泽 何家庆 王再岚 李红丽 李静敏 《安徽大学学报(自然科学版)》 CAS 北大核心 2011年第6期98-107,共10页
濒危植物保护生物学研究已成为生态学领域的研究热点之一.为了廓清我国特有濒危植物醉翁榆(Ulmus gaussenii)与相近榆属植物的系统地位,用分子标记ITS和atpB-rbcL手段分析醉翁榆与其他榆属相关种的亲缘关系,结果表明:24种榆属植物atpB-r... 濒危植物保护生物学研究已成为生态学领域的研究热点之一.为了廓清我国特有濒危植物醉翁榆(Ulmus gaussenii)与相近榆属植物的系统地位,用分子标记ITS和atpB-rbcL手段分析醉翁榆与其他榆属相关种的亲缘关系,结果表明:24种榆属植物atpB-rbcL扩增片段长度为735~834 bp,G+C的含量在29.3%~31.6%之间,并没有发生很大的偏差;基于ITS(包括5.8 s)扩增片段,醉翁榆与黑榆系的毛果旱榆、栓枝春榆以及榆系中的大果榆、脱皮榆(U.lamellosa)相近;基于atpB-rbcL扩增片段,醉翁榆与榆系中的大果榆、脱皮榆以及明陵榆的关系比较近,两种标记都表现出醉翁榆与黑榆系中变种毛果旱榆、栓枝春榆的关系比较近;atpB-rbcL的NJ和Bayesian树完全支持榆属组下的分类以及榆属系统的演化关系. 展开更多
关键词 醉翁榆 遗传随机性 atpb-rbcl ITS 系统树
下载PDF
Research Progress of Sugarcane Chloroplast Genome
20
作者 吴杨 周会 《Agricultural Science & Technology》 CAS 2013年第12期1693-1697,1706,共6页
Along with the development of modern molecular biology technologies, complete chloroplast genomes have been sequenced in various plant species to date, and the structure, function and expression of these genes have be... Along with the development of modern molecular biology technologies, complete chloroplast genomes have been sequenced in various plant species to date, and the structure, function and expression of these genes have been deter-mined. The chloroplast genome structure in most higher plants is stable, since the gene number, arrangement and composition are conservative. The determination of sugarcane chloroplast genome sequence laid a good foundation for sugarcane chloroplast related research. This article gives a review on the research progress of sugarcane chloroplast genome through the chloroplast genome map, gene structure, function, chloroplast RNA editing, and phylogenetic analysis in Saccharum and relat-ed genera. This study held great potential to clarify more directions in researches, including sugarcane chloroplast genetic transformation, complete chloroplast nu-cleotide sequence determination in Saccharum and closely related genera, cpSSRs development and application. 展开更多
关键词 SUGARCANE chloroplast genome RNA editing Phylogenetic analysis chloroplast transformation
下载PDF
上一页 1 2 122 下一页 到第
使用帮助 返回顶部