OBJECTIVE： To study the effects of berberine on ac- tivity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549. METHODS： N-acetyltransferase antibodies wereprepared. The human lung cancer ...OBJECTIVE： To study the effects of berberine on ac- tivity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549. METHODS： N-acetyltransferase antibodies wereprepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate, and divided into the control group, and berberine 0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups, with 3 wells for each group. 24 h later, A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse tran- scription polymerase chain reaction. RESULTS The N-acetyltransferase content in hu- man lung cancer A549 cells decreased with the in- creasing of berberine concentration, significantly lower than that in the control group （P〈O.05 or P〈 0.001）; and the mRNA expression of N-acetyltrans- ferase also decreased with the increasing of berber- ine concentration, significantly lower in Huanglian- su treatment groups （P〈0.O01）. CONCLUSION： Berberine can inhibit the activity of N-acetyltransferase in human lung cancer cell line A549, and shows negative correlations of dose and time in a certain extent. The inhibited gene expres- sion of N-acetyltransferase in human lung cancer A549 cell may probably represent one of the mech- anisms for its antineoplastic effect.展开更多
基金Supported by Xiamen Science and Technology Key Program Plan Grant(No.3502Z20100006)Scientific Research Starting Foundation for New Teacher of Xiamen University(No.ZK1014)
文摘OBJECTIVE： To study the effects of berberine on ac- tivity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549. METHODS： N-acetyltransferase antibodies wereprepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate, and divided into the control group, and berberine 0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups, with 3 wells for each group. 24 h later, A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse tran- scription polymerase chain reaction. RESULTS The N-acetyltransferase content in hu- man lung cancer A549 cells decreased with the in- creasing of berberine concentration, significantly lower than that in the control group （P〈O.05 or P〈 0.001）; and the mRNA expression of N-acetyltrans- ferase also decreased with the increasing of berber- ine concentration, significantly lower in Huanglian- su treatment groups （P〈0.O01）. CONCLUSION： Berberine can inhibit the activity of N-acetyltransferase in human lung cancer cell line A549, and shows negative correlations of dose and time in a certain extent. The inhibited gene expres- sion of N-acetyltransferase in human lung cancer A549 cell may probably represent one of the mech- anisms for its antineoplastic effect.
