Objective:To investigate the mutagenic potential of Trois using the bacterial reverse mutation assay(Ames test)and in vitro chromosomal aberration test.Methods:The ability of Trois to induce reverse mutations was eval...Objective:To investigate the mutagenic potential of Trois using the bacterial reverse mutation assay(Ames test)and in vitro chromosomal aberration test.Methods:The ability of Trois to induce reverse mutations was evaluated in Salmonella lyphimurium(TA 98,TA100,TAI535 and TA1537)and Escherichia coli(WP2 uvrA)with and without metabolic activation system(S9 mix)at the dose range of 313 to 5000μg/plate.Chromosomal aberrations were evaluated in Chinese hamster lung(CHL)cell line at the dose levels of 15,7.5,3.7,1.9 and 0.9 mg/mL in the absence and presence of S9 mix.Results:There were no increases in the number of revertant colonies at any concentrations of Trois used in the study with and without S9 mix in all tester strains.Trois did not produce any structural aberration in CHL cells in the presence or absence of S9 mix.Conclusions:Results of this study suggest that Trois is non-mutagenic.展开更多
Propoxur is a widely used dithiocarbamate insecticide. In this study, the clastogenic effect of propoxur has been evaluated using chromosomal aberration assay in mouse bone marrow cells. Single i. p. administration of...Propoxur is a widely used dithiocarbamate insecticide. In this study, the clastogenic effect of propoxur has been evaluated using chromosomal aberration assay in mouse bone marrow cells. Single i. p. administration of propoxur, at 25 mg/kg b.wt., a maximum tolerated dose (MTD) and 12 .5mg/kg b.wt (50% of MTD) have significantly induced different types of aberrations after 24 h of treatment. The aberrations were dose and time dependent and reached a maximum after 24 h of exposure. The sresult suggest a genotoxic potential of propoxur.展开更多
基金supported by Venus Medicine Research Centre,Werne,Germany(Grant No.VPG-115-2012)
文摘Objective:To investigate the mutagenic potential of Trois using the bacterial reverse mutation assay(Ames test)and in vitro chromosomal aberration test.Methods:The ability of Trois to induce reverse mutations was evaluated in Salmonella lyphimurium(TA 98,TA100,TAI535 and TA1537)and Escherichia coli(WP2 uvrA)with and without metabolic activation system(S9 mix)at the dose range of 313 to 5000μg/plate.Chromosomal aberrations were evaluated in Chinese hamster lung(CHL)cell line at the dose levels of 15,7.5,3.7,1.9 and 0.9 mg/mL in the absence and presence of S9 mix.Results:There were no increases in the number of revertant colonies at any concentrations of Trois used in the study with and without S9 mix in all tester strains.Trois did not produce any structural aberration in CHL cells in the presence or absence of S9 mix.Conclusions:Results of this study suggest that Trois is non-mutagenic.
文摘Propoxur is a widely used dithiocarbamate insecticide. In this study, the clastogenic effect of propoxur has been evaluated using chromosomal aberration assay in mouse bone marrow cells. Single i. p. administration of propoxur, at 25 mg/kg b.wt., a maximum tolerated dose (MTD) and 12 .5mg/kg b.wt (50% of MTD) have significantly induced different types of aberrations after 24 h of treatment. The aberrations were dose and time dependent and reached a maximum after 24 h of exposure. The sresult suggest a genotoxic potential of propoxur.
