AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting cells.METHODS:Cinnamon extract was used to treat murine macrophage cel...AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting cells.METHODS:Cinnamon extract was used to treat murine macrophage cell line(Raw 264.7),mouse primary antigen-presenting cells(APCs,MHCII+) and CD11c+dendritic cells to analyze the effects of cinnamon extract on APC function.The mechanisms of action of cinnamon extract on APCs were investigated by analyzing cytokine production,and expression of MHC antigens and co-stimulatory molecules by quantitative real-time PCR and flow cytometry.In addition,the effect of cinnamon extract on antigen presentation capacity and APC-dependent T-cell differentiation were analyzed by [H3]-thymidine incorporation and cytokine analysis,respectively.To confirm the anti-inflammatory effects of cinnamon extract in vivo,cinnamon or PBS was orally administered to mice for 20 d followed by induction of experimental colitis with 2,4,6 trinitrobenzenesulfonic acid.The protective effects of cinnamon extract against experimental colitis were measured by checking clinical symptoms,histological analysis and cytokine expression prof iles in inflamed tissue.RESULTS:Treatment with cinnamon extract inhibited maturation of MHCII+ APCs or CD11c+ dendritic cells(DCs) by suppressing expression of co-stimulatory molecules(B7.1,B7.2,ICOS-L),MHCII and cyclooxygenase(COX)-2.Cinnamon extract induced regulatory DCs(rDCs) that produce low levels of pro-inflammatory cytokines [interleukin(IL)-1β,IL-6,IL-12,interferon(IFN)-γ and tumor necrosis factor(TNF)-α] while expressing high levels of immunoregulatory cytokines(IL-10 and transforming growth factor-β).In addition,rDCs generated by cinnamon extract inhibited APC-dependent T-cell proliferation,and converted CD4+ T cells into IL-10high CD4+ T cells.Furthermore,oral administration of cinnamon extract inhibited development and progression of intestinal colitis by inhibiting expression of COX-2 and pro-inflammatory cytokines(IL-1β,IFN-γ and TNF-α),while enhancing IL-10 levels.CONCLUSION:Our study suggests the potential of cinnamon extract as an anti-inflammatory agent by targeting the generation of regulatory APCs and IL-10+ regulatory T cells.展开更多
The contribution of cinnamon extract on leaching of aluminum (Al) cook wares was investigated using two aluminum alloys (Indian and Egyptian) and pure Al. The cinnamon (Cin) was extracted by heating the Cin sticks at ...The contribution of cinnamon extract on leaching of aluminum (Al) cook wares was investigated using two aluminum alloys (Indian and Egyptian) and pure Al. The cinnamon (Cin) was extracted by heating the Cin sticks at 90°C in distilled water for an hour to make the 10% stock solution. This study was done in aqueous solutions in presence and absence of 1% NaCl using weight loss at 90°C. Moreover surface study (SEM and EDX) and electrochemical methods (Open Circuit Potential and Tafel plot) were applied. The addition of Cin solutions to 1% NaCl decreased the corrosion rates in weight loss and electrochemical method compared to 1% NaCl solutions which showed an inhibitive property of Cin solution. The inhibition was found to obey the modified Langmuir isotherm with a negative Langmuir ΔGads indicating the spontaneous nature of adsorption even at 90°C. The EDX surface analysis of the Al surface immersed in Cin + NaCl revealed the composition of the pits formed. From Tafel method, it was found that the corrosion current density of pure Al was more than that of the Egyptian alloy. The apparent activation energy values for Cin, Cin + NaCl and NaCl solution were evaluated and discussed.展开更多
滤泡辅助性T细胞(Follicular helper CD4 T cells,Tfh)是重要的CD4^(+)T淋巴细胞亚群之一,与多种疾病的发生和发展密切相关,其参与疾病发生的机制尚有待深入研究。该研究将分离小鼠脾脏细胞,应用贝克曼Moflo分选系统分选出CD4^(+)T淋巴...滤泡辅助性T细胞(Follicular helper CD4 T cells,Tfh)是重要的CD4^(+)T淋巴细胞亚群之一,与多种疾病的发生和发展密切相关,其参与疾病发生的机制尚有待深入研究。该研究将分离小鼠脾脏细胞,应用贝克曼Moflo分选系统分选出CD4^(+)T淋巴细胞。使用细胞因子刺激Tfh细胞分化并检测其分化比例,同时采用流式细胞术检测和分析肉桂活性成分对滤泡辅助性T细胞分化的影响。结果表明,贝克曼Moflo XDP分选系统可以显著提高CD4^(+)T淋巴细胞的纯度,且肉桂活性成分可以显著抑制Tfh细胞分化。为新药在Tfh细胞的开发提供一定实验基础,并且为研发肉桂作为新的增强免疫疗效药物提供可靠的实验依据。展开更多
探讨桂皮多酚的最佳提取工艺条件及多酚的稳定性。分别通过单因素实验和正交实验设计优化桂皮多酚的最佳提取工艺;并通过改变介质温度、pH、氧化剂浓度、还原剂浓度,考察桂皮多酚的稳定性。结果表明,桂皮多酚的最佳提取工艺条件为:提取...探讨桂皮多酚的最佳提取工艺条件及多酚的稳定性。分别通过单因素实验和正交实验设计优化桂皮多酚的最佳提取工艺;并通过改变介质温度、pH、氧化剂浓度、还原剂浓度,考察桂皮多酚的稳定性。结果表明,桂皮多酚的最佳提取工艺条件为:提取温度70℃,料液比1∶40(g/mL),乙醇体积分数60%,提取时间70min,在此条件下多酚提取率为3.87%;桂皮多酚在温度低于40℃、中性偏弱酸条件下比较稳定,低浓度的还原剂Na 2 SO 3对桂皮多酚有一定的保护作用,低浓度的氧化剂H 2 O 2对桂皮多酚影响不大,桂皮多酚具有一定的抗氧化性。展开更多
基金Supported by Grants from the BioGreen 21 Program, Rural Development Administration (PJ007054)Regional Technology Innovation Program of the MOCIE (RTI05-01-01)Korea Healthcare Technology R&D Project, Ministry of Health and Welfare (A080588-20)
文摘AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting cells.METHODS:Cinnamon extract was used to treat murine macrophage cell line(Raw 264.7),mouse primary antigen-presenting cells(APCs,MHCII+) and CD11c+dendritic cells to analyze the effects of cinnamon extract on APC function.The mechanisms of action of cinnamon extract on APCs were investigated by analyzing cytokine production,and expression of MHC antigens and co-stimulatory molecules by quantitative real-time PCR and flow cytometry.In addition,the effect of cinnamon extract on antigen presentation capacity and APC-dependent T-cell differentiation were analyzed by [H3]-thymidine incorporation and cytokine analysis,respectively.To confirm the anti-inflammatory effects of cinnamon extract in vivo,cinnamon or PBS was orally administered to mice for 20 d followed by induction of experimental colitis with 2,4,6 trinitrobenzenesulfonic acid.The protective effects of cinnamon extract against experimental colitis were measured by checking clinical symptoms,histological analysis and cytokine expression prof iles in inflamed tissue.RESULTS:Treatment with cinnamon extract inhibited maturation of MHCII+ APCs or CD11c+ dendritic cells(DCs) by suppressing expression of co-stimulatory molecules(B7.1,B7.2,ICOS-L),MHCII and cyclooxygenase(COX)-2.Cinnamon extract induced regulatory DCs(rDCs) that produce low levels of pro-inflammatory cytokines [interleukin(IL)-1β,IL-6,IL-12,interferon(IFN)-γ and tumor necrosis factor(TNF)-α] while expressing high levels of immunoregulatory cytokines(IL-10 and transforming growth factor-β).In addition,rDCs generated by cinnamon extract inhibited APC-dependent T-cell proliferation,and converted CD4+ T cells into IL-10high CD4+ T cells.Furthermore,oral administration of cinnamon extract inhibited development and progression of intestinal colitis by inhibiting expression of COX-2 and pro-inflammatory cytokines(IL-1β,IFN-γ and TNF-α),while enhancing IL-10 levels.CONCLUSION:Our study suggests the potential of cinnamon extract as an anti-inflammatory agent by targeting the generation of regulatory APCs and IL-10+ regulatory T cells.
文摘The contribution of cinnamon extract on leaching of aluminum (Al) cook wares was investigated using two aluminum alloys (Indian and Egyptian) and pure Al. The cinnamon (Cin) was extracted by heating the Cin sticks at 90°C in distilled water for an hour to make the 10% stock solution. This study was done in aqueous solutions in presence and absence of 1% NaCl using weight loss at 90°C. Moreover surface study (SEM and EDX) and electrochemical methods (Open Circuit Potential and Tafel plot) were applied. The addition of Cin solutions to 1% NaCl decreased the corrosion rates in weight loss and electrochemical method compared to 1% NaCl solutions which showed an inhibitive property of Cin solution. The inhibition was found to obey the modified Langmuir isotherm with a negative Langmuir ΔGads indicating the spontaneous nature of adsorption even at 90°C. The EDX surface analysis of the Al surface immersed in Cin + NaCl revealed the composition of the pits formed. From Tafel method, it was found that the corrosion current density of pure Al was more than that of the Egyptian alloy. The apparent activation energy values for Cin, Cin + NaCl and NaCl solution were evaluated and discussed.
文摘探讨桂皮多酚的最佳提取工艺条件及多酚的稳定性。分别通过单因素实验和正交实验设计优化桂皮多酚的最佳提取工艺;并通过改变介质温度、pH、氧化剂浓度、还原剂浓度,考察桂皮多酚的稳定性。结果表明,桂皮多酚的最佳提取工艺条件为:提取温度70℃,料液比1∶40(g/mL),乙醇体积分数60%,提取时间70min,在此条件下多酚提取率为3.87%;桂皮多酚在温度低于40℃、中性偏弱酸条件下比较稳定,低浓度的还原剂Na 2 SO 3对桂皮多酚有一定的保护作用,低浓度的氧化剂H 2 O 2对桂皮多酚影响不大,桂皮多酚具有一定的抗氧化性。
