Interactions between Cd 2+ ions and salmon sperm DNA were investigated via UV Vis, Circular Dichroism(CD) and FT Raman spectroscopies. The experimental results show that Cd 2+ ions can coordinate with the phosphate gr...Interactions between Cd 2+ ions and salmon sperm DNA were investigated via UV Vis, Circular Dichroism(CD) and FT Raman spectroscopies. The experimental results show that Cd 2+ ions can coordinate with the phosphate groups of DNA and thus induce conformation changes of the DNA from B to A type.展开更多
Detailed circular dichroism(CD) and Fourier transform infrared(FTIR) studies have been carried out to monitor thermal unfolding of horseradish peroxidase isoenzyme C(HRP) inhibited by CN -(HRP CN). The results suggest...Detailed circular dichroism(CD) and Fourier transform infrared(FTIR) studies have been carried out to monitor thermal unfolding of horseradish peroxidase isoenzyme C(HRP) inhibited by CN -(HRP CN). The results suggest that HRP CN is quite different from native HRP with different spin states of Fe of heme and different coordinated states. Cyanide becomes the sixth ligand of Fe(Ⅲ) of heme and the hydrogen binding network is destroyed partly at the same time, which cause the drastic decrease of thermal stability of HRP. The FTIR and Soret CD spectra analysis demonstrate that during the heating process there is an intermediate state(I) which has both partly destroyed secondary and tertiary structures of native HRP, then it is the appearance of protein aggregation state(A) after fully unfolding. The unfolding pathway thus can be shown as follows: IIUA.展开更多
文摘Interactions between Cd 2+ ions and salmon sperm DNA were investigated via UV Vis, Circular Dichroism(CD) and FT Raman spectroscopies. The experimental results show that Cd 2+ ions can coordinate with the phosphate groups of DNA and thus induce conformation changes of the DNA from B to A type.
文摘Detailed circular dichroism(CD) and Fourier transform infrared(FTIR) studies have been carried out to monitor thermal unfolding of horseradish peroxidase isoenzyme C(HRP) inhibited by CN -(HRP CN). The results suggest that HRP CN is quite different from native HRP with different spin states of Fe of heme and different coordinated states. Cyanide becomes the sixth ligand of Fe(Ⅲ) of heme and the hydrogen binding network is destroyed partly at the same time, which cause the drastic decrease of thermal stability of HRP. The FTIR and Soret CD spectra analysis demonstrate that during the heating process there is an intermediate state(I) which has both partly destroyed secondary and tertiary structures of native HRP, then it is the appearance of protein aggregation state(A) after fully unfolding. The unfolding pathway thus can be shown as follows: IIUA.
