目的通过检测血清微小RNA-126(miR-126)在急性脑梗死介入取栓术治疗前的改变,探讨其与患者预后的相关性。方法回顾性分析2019年1月至2021年12月在湖州市第一人民医院行介入取栓术的脑梗死患者101例,随访2个月,根据改良Rankin评分(modifi...目的通过检测血清微小RNA-126(miR-126)在急性脑梗死介入取栓术治疗前的改变,探讨其与患者预后的相关性。方法回顾性分析2019年1月至2021年12月在湖州市第一人民医院行介入取栓术的脑梗死患者101例,随访2个月,根据改良Rankin评分(modified Rankin scale,mRS)分为预后良好组(mRS≤2分,56例)和预后不良组(mRS>2分,45例),比较两组患者的临床资料及miR-126在取栓术前的差异,分析血清miR-126改变对脑梗死患者预后的影响。结果取栓术前血清miR-126水平在预后良好组明显高于预后不良组[(9.31±2.14)vs.(1.36±0.28),P<0.01],且miR-126与美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评分呈负相关(r=–0.737,P<0.01),与侧支循环良好呈正相关(r=0.645,P<0.01),创建miR-126预测脑梗死取栓术预后的受试者操作特征曲线下面积为0.818,最佳截断值敏感度78.9%、特异性86.0%。结论血清miR-126水平在脑梗死取栓术前的改变与患者预后可能相关,可作为早期预测脑梗死介入治疗预后的标志物。展开更多
目的探讨血浆循环microRNA-126浓度与非小细胞肺癌(NSCLC)的相关性及其诊断价值。方法采用实时荧光定量PCR方法检测130例NSCLC患者和作为对照的170例健康人的血浆循环microRNA-126浓度。结果对照组血浆循环microRNA-126浓度显著高于NSCL...目的探讨血浆循环microRNA-126浓度与非小细胞肺癌(NSCLC)的相关性及其诊断价值。方法采用实时荧光定量PCR方法检测130例NSCLC患者和作为对照的170例健康人的血浆循环microRNA-126浓度。结果对照组血浆循环microRNA-126浓度显著高于NSCLC组(P<0.01)。血浆循环microRNA-126浓度ROC曲线下面积(area under theROC curve,AUC)为0.758,95%CI为0.704～0.811(P<0.01)。当血浆microRNA-126浓度为1051 fmol/L时,血浆microRNA-126对NSCLC诊断的特异度为90.0%,灵敏度为46.4%。其浓度与患者的年龄、性别、吸烟,病理分型和分期均无显著的相关性(P>0.05)。结论血浆循环microRNA-126浓度对NSCLC有一定的临床诊断意义,联合其他血浆microRNA或肿瘤标志物,如神经元特异性烯醇化酶、癌胚抗原和细胞角蛋白片段21-1,可以弥补单一检测的局限性,以提高肺癌的阳性检出率,为临床提供可靠的依据。展开更多
AIM：To investigate the content of serum micro RNA-126（mi R-126） and its role in screening retinal endothelial injury and early diagnosis of proliferative diabetic retinopathy.METHODS：The study included 184 serum s...AIM：To investigate the content of serum micro RNA-126（mi R-126） and its role in screening retinal endothelial injury and early diagnosis of proliferative diabetic retinopathy.METHODS：The study included 184 serum samples,59 samples from healthy individuals,44 samples from diabetes mellitus（DM） patients without diabetic retinopathy（NDR）,42 from non-proliferative diabetic retinopathy（NPDR） patients and 39 samples from proliferative diabetic retinopathy（PDR） patients.The expression of mi R-126 was evaluated using a real-time quantitative polymerase chain reaction.RESULTS：The serum content of mi R-126 declined as the damage degree in the retina.There was significant difference between the two retinopathy groups（P〈0.001）.No difference was observed in mi R-126 content between healthy individuals and NDR patients（P〉0.05）.Receiver operating characteristic curve（ROC） analyses indicated that serum mi R-126 had significant diagnostic value for PDR.It yielded an area under the curve（AUC） of ROC of 0.976 with 81.21% sensitivity and 90.34% specificity in discriminating PDR from healthy controls,and an AUC of ROC of 0.919 with 84.75% sensitivity and 94.41% specificity in discriminating NDR and NPDR from healthy controls.When the diagnostic threshold was greater than or equal to 8.43,there was an increase in the possibility of NPDR.When the content of mi R-126 was less than or equal to 5.02,the possibility of the occurrence of PDR increased.CONCLUSION：Serum mi R-126 can serve as a non-invasive biomarker for screening retinal endothelial injury and early diagnosis PDR.展开更多
Objective:To study the role of microRNA-126 in the development of lung cancer.Methods:The biological function of microRNA-126 was delected using EdU assay and CCK-8 assay:the target gene of microRNA-126 was analyzed u...Objective:To study the role of microRNA-126 in the development of lung cancer.Methods:The biological function of microRNA-126 was delected using EdU assay and CCK-8 assay:the target gene of microRNA-126 was analyzed using real time RT-PCR and Western blot assay.Results:In A549 cell line,overexpression of microRNA-126 inhibils the prolileralion rate:VEGF is the target gene of microRNA-126:microRNA-126 exerts its function via regulating VEGF protein level.Conclusions:microRNA-126 inhibits the proliferation in A549 cell line.展开更多
文摘目的通过检测血清微小RNA-126(miR-126)在急性脑梗死介入取栓术治疗前的改变,探讨其与患者预后的相关性。方法回顾性分析2019年1月至2021年12月在湖州市第一人民医院行介入取栓术的脑梗死患者101例,随访2个月,根据改良Rankin评分(modified Rankin scale,mRS)分为预后良好组(mRS≤2分,56例)和预后不良组(mRS>2分,45例),比较两组患者的临床资料及miR-126在取栓术前的差异,分析血清miR-126改变对脑梗死患者预后的影响。结果取栓术前血清miR-126水平在预后良好组明显高于预后不良组[(9.31±2.14)vs.(1.36±0.28),P<0.01],且miR-126与美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评分呈负相关(r=–0.737,P<0.01),与侧支循环良好呈正相关(r=0.645,P<0.01),创建miR-126预测脑梗死取栓术预后的受试者操作特征曲线下面积为0.818,最佳截断值敏感度78.9%、特异性86.0%。结论血清miR-126水平在脑梗死取栓术前的改变与患者预后可能相关,可作为早期预测脑梗死介入治疗预后的标志物。
文摘目的探讨血浆循环microRNA-126浓度与非小细胞肺癌(NSCLC)的相关性及其诊断价值。方法采用实时荧光定量PCR方法检测130例NSCLC患者和作为对照的170例健康人的血浆循环microRNA-126浓度。结果对照组血浆循环microRNA-126浓度显著高于NSCLC组(P<0.01)。血浆循环microRNA-126浓度ROC曲线下面积(area under theROC curve,AUC)为0.758,95%CI为0.704～0.811(P<0.01)。当血浆microRNA-126浓度为1051 fmol/L时,血浆microRNA-126对NSCLC诊断的特异度为90.0%,灵敏度为46.4%。其浓度与患者的年龄、性别、吸烟,病理分型和分期均无显著的相关性(P>0.05)。结论血浆循环microRNA-126浓度对NSCLC有一定的临床诊断意义,联合其他血浆microRNA或肿瘤标志物,如神经元特异性烯醇化酶、癌胚抗原和细胞角蛋白片段21-1,可以弥补单一检测的局限性,以提高肺癌的阳性检出率,为临床提供可靠的依据。
基金Supported by Science and Technology Planning Project of Guangdong Province(No.201 4A020212631)Science and Technology Planning Project of Guangzhou(No.201604020105)Science and Technology Planning Project of Tianhe District,Guangzhou(No.201504KW023)
文摘AIM：To investigate the content of serum micro RNA-126（mi R-126） and its role in screening retinal endothelial injury and early diagnosis of proliferative diabetic retinopathy.METHODS：The study included 184 serum samples,59 samples from healthy individuals,44 samples from diabetes mellitus（DM） patients without diabetic retinopathy（NDR）,42 from non-proliferative diabetic retinopathy（NPDR） patients and 39 samples from proliferative diabetic retinopathy（PDR） patients.The expression of mi R-126 was evaluated using a real-time quantitative polymerase chain reaction.RESULTS：The serum content of mi R-126 declined as the damage degree in the retina.There was significant difference between the two retinopathy groups（P〈0.001）.No difference was observed in mi R-126 content between healthy individuals and NDR patients（P〉0.05）.Receiver operating characteristic curve（ROC） analyses indicated that serum mi R-126 had significant diagnostic value for PDR.It yielded an area under the curve（AUC） of ROC of 0.976 with 81.21% sensitivity and 90.34% specificity in discriminating PDR from healthy controls,and an AUC of ROC of 0.919 with 84.75% sensitivity and 94.41% specificity in discriminating NDR and NPDR from healthy controls.When the diagnostic threshold was greater than or equal to 8.43,there was an increase in the possibility of NPDR.When the content of mi R-126 was less than or equal to 5.02,the possibility of the occurrence of PDR increased.CONCLUSION：Serum mi R-126 can serve as a non-invasive biomarker for screening retinal endothelial injury and early diagnosis PDR.
基金supported by National Natutal Science Foundation of China(81073036)
文摘Objective:To study the role of microRNA-126 in the development of lung cancer.Methods:The biological function of microRNA-126 was delected using EdU assay and CCK-8 assay:the target gene of microRNA-126 was analyzed using real time RT-PCR and Western blot assay.Results:In A549 cell line,overexpression of microRNA-126 inhibils the prolileralion rate:VEGF is the target gene of microRNA-126:microRNA-126 exerts its function via regulating VEGF protein level.Conclusions:microRNA-126 inhibits the proliferation in A549 cell line.
