Rapid differential identification of Mycobacterium species is essential for effective diagnosis and management of mycobacteriosis. The aim of this study was to develop a novel multiplex probe array based on the 16S-23...Rapid differential identification of Mycobacterium species is essential for effective diagnosis and management of mycobacteriosis. The aim of this study was to develop a novel multiplex probe array based on the 16S-23S rRNA gene internal transcribed spacer sequence for the genotyping of mycobacteria to the species level. A pair of primers and a set of genus- and species-specific probes were designed from the conserved and polymorphic regions of the 16S rRNA gene, internal transcribed spacer, and 23S rRNA gene sequences of mycobacteria. We used a novel multiplex probe array for identification of 266 clinical specimens obtained from patients with mycobaterial infection. The results showed that the overall specificity and sensitivity of our novel probe array were both 100% for the genus-specific probe and Mycobacterium tuberculosis complex- specific probe. There were 79.3 % (23/29) of nontuberculous mycobacteria which could be identified to the species level directly in the specimens from China. Some intraspecies heterogeneity in M. avium, M. intracellulare, M. chelonae and M. abscessus was observed. With the increase of sequences of internal transcribed spacer and numbers of whole microbial genomes, and further optimization of probes, the multiplex probe army will become a promising tool for the rapid and accurate identification of mycobacteria in ordinary clinical laboratories.展开更多
The clinical manifestations of paroxysmal sympathetic hyperactivity(PSH)after severe craniocerebral injury are very similar to those of grand mal seizures,which are easily misdiagnosed in the early stage,resulting in ...The clinical manifestations of paroxysmal sympathetic hyperactivity(PSH)after severe craniocerebral injury are very similar to those of grand mal seizures,which are easily misdiagnosed in the early stage,resulting in further irreversible brain damage.By comparing the similarities in clinical manifestations,seizure mechanism,drug treatment,and other differences of the above two,PSH can be identified clinically early.In this article,the common clinical manifestations and differences between PSH and grand mal seizures are listed by referring to relevant literature and combined with the nursing observation of PSH patients in the Department of Neurosurgery,the First Affiliated Hospital,Zhejiang University,School of Medicine in the past two years.Our research shows that PSH is a clinical syndrome that appears after a variety of acute brain injuries and is mainly characterized by hyperexcitatory sympathetic nerves.The clinical manifestations of PSH are complex and difficult to identify.Raising awareness of the syndrome is helpful for its early diagnosis and treatment.展开更多
文摘Rapid differential identification of Mycobacterium species is essential for effective diagnosis and management of mycobacteriosis. The aim of this study was to develop a novel multiplex probe array based on the 16S-23S rRNA gene internal transcribed spacer sequence for the genotyping of mycobacteria to the species level. A pair of primers and a set of genus- and species-specific probes were designed from the conserved and polymorphic regions of the 16S rRNA gene, internal transcribed spacer, and 23S rRNA gene sequences of mycobacteria. We used a novel multiplex probe array for identification of 266 clinical specimens obtained from patients with mycobaterial infection. The results showed that the overall specificity and sensitivity of our novel probe array were both 100% for the genus-specific probe and Mycobacterium tuberculosis complex- specific probe. There were 79.3 % (23/29) of nontuberculous mycobacteria which could be identified to the species level directly in the specimens from China. Some intraspecies heterogeneity in M. avium, M. intracellulare, M. chelonae and M. abscessus was observed. With the increase of sequences of internal transcribed spacer and numbers of whole microbial genomes, and further optimization of probes, the multiplex probe army will become a promising tool for the rapid and accurate identification of mycobacteria in ordinary clinical laboratories.
文摘The clinical manifestations of paroxysmal sympathetic hyperactivity(PSH)after severe craniocerebral injury are very similar to those of grand mal seizures,which are easily misdiagnosed in the early stage,resulting in further irreversible brain damage.By comparing the similarities in clinical manifestations,seizure mechanism,drug treatment,and other differences of the above two,PSH can be identified clinically early.In this article,the common clinical manifestations and differences between PSH and grand mal seizures are listed by referring to relevant literature and combined with the nursing observation of PSH patients in the Department of Neurosurgery,the First Affiliated Hospital,Zhejiang University,School of Medicine in the past two years.Our research shows that PSH is a clinical syndrome that appears after a variety of acute brain injuries and is mainly characterized by hyperexcitatory sympathetic nerves.The clinical manifestations of PSH are complex and difficult to identify.Raising awareness of the syndrome is helpful for its early diagnosis and treatment.