AIM To prospectively investigate the efficacy and safety of clipflap assisted endoscopic submucosal dissection(ESD) for gastric tumors.METHODS From May 2015 to October 2016, we enrolled 104 patients with gastric cance...AIM To prospectively investigate the efficacy and safety of clipflap assisted endoscopic submucosal dissection(ESD) for gastric tumors.METHODS From May 2015 to October 2016, we enrolled 104 patients with gastric cancer or adenoma scheduled for ESD at Shiga University of Medical Science Hospital. We randomized patients into two subgroups using the minimization method based on location of the tumor(upper, middle or lower third of the stomach), tumor size(< 20 mm or > 20 mm) and ulcer status: ESD using an endoclip(the clip-flap group) and ESD without an endoclip(the conventional group). Therapeutic efficacy(procedure time) and safety(complication: Gastrointestinal bleeding and perforation) were assessed. RESULTS En bloc resection was performed in all patients. Four patients had delayed bleeding(3.8%) and two had perforation(1.9%). No significant differences in en bloc resection rate(conventional group: 100%, clip flap group: 100%), curative endoscopic resection rate(conventional group: 90.9%, clip flap group: 89.8%, P = 0.85), procedure time(conventional group: 70.8 ± 46.2 min, clip flap group: 74.7 ± 53.3 min, P = 0.69), area of resected specimen(conventional group: 884.6 ± 792.1 mm^2, clip flap group: 1006.4 ± 1004.8 mm^2, P = 0.49), delayed bleeding rate(conventional group: 5.5%, clip flap group: 2.0%, P = 0.49), or perforation rate(conventional group: 1.8%, clip flap group: 2.0%, P = 0.93) were found between the two groups. Lessexperienced endoscopists did not show any differences in procedure time between the two groups.CONCLUSION For patients with early-stage gastric tumors, the clipflap method has no advantage in efficacy or safety compared with the conventional method.展开更多
This paper presents a novel robust S transform algorithm based on the clipping method to process signals corrupted by impulsive noise.The proposed algorithm is introduced to determine the clipping threshold value acco...This paper presents a novel robust S transform algorithm based on the clipping method to process signals corrupted by impulsive noise.The proposed algorithm is introduced to determine the clipping threshold value according to the characteristics of the signal samples.Signals in various impulsive noise models are considered to illustrate that the robust S transform can achieve better performance than the standard S transform.Moreover,mean square errors for instantaneous frequency estimation of the robust S transform are compared with that of the standard S transform,showing that the robust S transform can achieve significantly improved instantaneous frequency estimation for the signals in impulsive noise.展开更多
BACKGROUND We recently developed a new endoscopic closure technique using only conventional endo-clips for colorectal lesions.Little is known about the feasibility of the endoscopic mucosa-submucosa clip closure metho...BACKGROUND We recently developed a new endoscopic closure technique using only conventional endo-clips for colorectal lesions.Little is known about the feasibility of the endoscopic mucosa-submucosa clip closure method for gastric lesions.AIM To elucidate the efficacy of the endoscopic mucosa-submucosa clip closure method after gastric endoscopic submucosal dissection(ESD).METHODS Twenty-two patients who underwent gastric ESD and mucosa-submucosa clip closure were included in this study.In this method,endo-clips are placed at the edges of a mucosal defect.Additional endo-clips are then applied in the same way to facilitate reduction of the defect size.Additional endo-clips are applied to both sides of the mucosal defect.Complete closure can be achieved.We have also developed a“location score”and“closure difficulty index”for assessment purposes.RESULTS Complete closure was achieved in 68.2%of the patients(15/22).The location score in the failure group was significantly larger than that in the complete closure group(P=0.023).The closure difficulty index in the failure group was significantly higher than that in the complete closure group(P=0.007).When the cutoff value of the closure difficulty index was set at 99,the high closure difficulty index predicted failure with a sensitivity of 57.1%,specificity of 100%,and accuracy of 86.3%.CONCLUSION The endoscopic mucosa-submucosa clip closure method was unreliable after gastric ESD,especially in cases with a high closure difficulty index.展开更多
Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition a...Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.Results:(1)The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2)There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3)The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.展开更多
Behavior and fitness are important ecological traits frequently measured in insect bioassays.A common method to measure them in soft-bodied herbivorous insects involves confining individuals to plant leaves using clip...Behavior and fitness are important ecological traits frequently measured in insect bioassays.A common method to measure them in soft-bodied herbivorous insects involves confining individuals to plant leaves using clip cages.Although studies have pre-viously highlighted the negative effects of clip cages on leaf physiology,little is known about the impact that using this confinement method has on insect fitness.The responses of different aphid genotypes/clones to different containment methods have not previously been investigated.Here we measured key fitness traits(intrinsic rate of natural increase,mean relative growth rate,time to reach reproductive adulthood and population doubling time)in the potato aphid,Macrosiphum euphorbiae Thomas(Hemiptera:Aphididae),when confined to plants using two methods:(1)clip cages to confine aphids to individual strawberry leaves and(2)a mesh bag to confine aphids to whole strawberry plants.Our study identified a strong negative impact on all the measured aphid fitness traits when using clip cages instead of mesh bags.We also identified genotype-specific differences in response to confinement method,where clip cage confinement differentially affected the fitness of a given aphid genotype compared to the same genotype on whole plants.These results suggest that clip cage use should be carefully considered when experiments seek to quantify insect fitness and that whole plants should be used wherever possible.Given the prevalence of clip cage use in insect bioassays,our results highlight the need for cau-tion when interpreting the existing literature as confinement method significantly impacts aphid fitness depending on their genotype.展开更多
目的研究前交通动脉瘤患者显微手术中不同的手术夹闭方式与预后的相关性。方法回顾性分析2018年1月~2020年12月徐州医科大学附属连云港医院收治的116例前交通动脉瘤患者的临床资料,根据患者出院后3~6个月的格拉斯哥预后评分(Glasgow com...目的研究前交通动脉瘤患者显微手术中不同的手术夹闭方式与预后的相关性。方法回顾性分析2018年1月~2020年12月徐州医科大学附属连云港医院收治的116例前交通动脉瘤患者的临床资料,根据患者出院后3~6个月的格拉斯哥预后评分(Glasgow coma scale,GCS评分)分为两组,即预后良好组(GCS评分为4~5分,n=93)和预后不良组(GCS评分为1~3分,n=23),采用多因素Logistic回归分析评价前交通动脉瘤预后不良的影响因素。结果GCS评分低、Hunt-Hess分级Ⅲ~Ⅳ级、改良Fisher分级Ⅲ~Ⅳ级、术后脑梗死、术后脑血管痉挛、术后脑积水和单线夹闭(动脉瘤夹夹片在一条虚拟线上)与预后不良相关(P<0.05)。多因素Logistic回归分析结果显示,Hunt-Hess分级≥Ⅲ级、术后脑梗死和术后脑积水与预后不良呈正相关(OR值分别为10.899、10.300、2.483、14.360,P<0.05)。多线夹闭(动脉瘤夹夹片在两条及以上虚拟线上)与预后不良呈负相关(OR=0.089,P<0.05)。结论Hunt-Hess分级≥Ⅲ级、术后脑梗死和术后脑积水是前交通动脉瘤显微手术患者预后不良的独立危险因素,多线夹闭是预后良好的一个保护因素。展开更多
文摘AIM To prospectively investigate the efficacy and safety of clipflap assisted endoscopic submucosal dissection(ESD) for gastric tumors.METHODS From May 2015 to October 2016, we enrolled 104 patients with gastric cancer or adenoma scheduled for ESD at Shiga University of Medical Science Hospital. We randomized patients into two subgroups using the minimization method based on location of the tumor(upper, middle or lower third of the stomach), tumor size(< 20 mm or > 20 mm) and ulcer status: ESD using an endoclip(the clip-flap group) and ESD without an endoclip(the conventional group). Therapeutic efficacy(procedure time) and safety(complication: Gastrointestinal bleeding and perforation) were assessed. RESULTS En bloc resection was performed in all patients. Four patients had delayed bleeding(3.8%) and two had perforation(1.9%). No significant differences in en bloc resection rate(conventional group: 100%, clip flap group: 100%), curative endoscopic resection rate(conventional group: 90.9%, clip flap group: 89.8%, P = 0.85), procedure time(conventional group: 70.8 ± 46.2 min, clip flap group: 74.7 ± 53.3 min, P = 0.69), area of resected specimen(conventional group: 884.6 ± 792.1 mm^2, clip flap group: 1006.4 ± 1004.8 mm^2, P = 0.49), delayed bleeding rate(conventional group: 5.5%, clip flap group: 2.0%, P = 0.49), or perforation rate(conventional group: 1.8%, clip flap group: 2.0%, P = 0.93) were found between the two groups. Lessexperienced endoscopists did not show any differences in procedure time between the two groups.CONCLUSION For patients with early-stage gastric tumors, the clipflap method has no advantage in efficacy or safety compared with the conventional method.
基金supported by the National Natural Science Foundation of China(6110216461272224)the Scientific Research Fund of Hangzhou Normal University(2011QDL021)
文摘This paper presents a novel robust S transform algorithm based on the clipping method to process signals corrupted by impulsive noise.The proposed algorithm is introduced to determine the clipping threshold value according to the characteristics of the signal samples.Signals in various impulsive noise models are considered to illustrate that the robust S transform can achieve better performance than the standard S transform.Moreover,mean square errors for instantaneous frequency estimation of the robust S transform are compared with that of the standard S transform,showing that the robust S transform can achieve significantly improved instantaneous frequency estimation for the signals in impulsive noise.
文摘BACKGROUND We recently developed a new endoscopic closure technique using only conventional endo-clips for colorectal lesions.Little is known about the feasibility of the endoscopic mucosa-submucosa clip closure method for gastric lesions.AIM To elucidate the efficacy of the endoscopic mucosa-submucosa clip closure method after gastric endoscopic submucosal dissection(ESD).METHODS Twenty-two patients who underwent gastric ESD and mucosa-submucosa clip closure were included in this study.In this method,endo-clips are placed at the edges of a mucosal defect.Additional endo-clips are then applied in the same way to facilitate reduction of the defect size.Additional endo-clips are applied to both sides of the mucosal defect.Complete closure can be achieved.We have also developed a“location score”and“closure difficulty index”for assessment purposes.RESULTS Complete closure was achieved in 68.2%of the patients(15/22).The location score in the failure group was significantly larger than that in the complete closure group(P=0.023).The closure difficulty index in the failure group was significantly higher than that in the complete closure group(P=0.007).When the cutoff value of the closure difficulty index was set at 99,the high closure difficulty index predicted failure with a sensitivity of 57.1%,specificity of 100%,and accuracy of 86.3%.CONCLUSION The endoscopic mucosa-submucosa clip closure method was unreliable after gastric ESD,especially in cases with a high closure difficulty index.
文摘Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.Results:(1)The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2)There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3)The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.
基金The authors would like to thank the Biotechnology and Biological Sciences Research Council and the Fruit Crop Science CTP for funding this projectthe Rural and Environment Science and Analytical Services(RESAS)Division of the Scottish Government through the Strategic Research Programme(2022-2027)+2 种基金the Underpinning National Capacity programme(Service 8.2 Maintenance of pest collections)for supporting the project through the James Hutton InstituteGaynor Malloch at the James Hutton Institute and Danielle Henderson-Holdings at Harper Adams University for their support with molecular workDr Edwin Harris at Harper Adams University for his support with data analysis.
文摘Behavior and fitness are important ecological traits frequently measured in insect bioassays.A common method to measure them in soft-bodied herbivorous insects involves confining individuals to plant leaves using clip cages.Although studies have pre-viously highlighted the negative effects of clip cages on leaf physiology,little is known about the impact that using this confinement method has on insect fitness.The responses of different aphid genotypes/clones to different containment methods have not previously been investigated.Here we measured key fitness traits(intrinsic rate of natural increase,mean relative growth rate,time to reach reproductive adulthood and population doubling time)in the potato aphid,Macrosiphum euphorbiae Thomas(Hemiptera:Aphididae),when confined to plants using two methods:(1)clip cages to confine aphids to individual strawberry leaves and(2)a mesh bag to confine aphids to whole strawberry plants.Our study identified a strong negative impact on all the measured aphid fitness traits when using clip cages instead of mesh bags.We also identified genotype-specific differences in response to confinement method,where clip cage confinement differentially affected the fitness of a given aphid genotype compared to the same genotype on whole plants.These results suggest that clip cage use should be carefully considered when experiments seek to quantify insect fitness and that whole plants should be used wherever possible.Given the prevalence of clip cage use in insect bioassays,our results highlight the need for cau-tion when interpreting the existing literature as confinement method significantly impacts aphid fitness depending on their genotype.
文摘目的研究前交通动脉瘤患者显微手术中不同的手术夹闭方式与预后的相关性。方法回顾性分析2018年1月~2020年12月徐州医科大学附属连云港医院收治的116例前交通动脉瘤患者的临床资料,根据患者出院后3~6个月的格拉斯哥预后评分(Glasgow coma scale,GCS评分)分为两组,即预后良好组(GCS评分为4~5分,n=93)和预后不良组(GCS评分为1~3分,n=23),采用多因素Logistic回归分析评价前交通动脉瘤预后不良的影响因素。结果GCS评分低、Hunt-Hess分级Ⅲ~Ⅳ级、改良Fisher分级Ⅲ~Ⅳ级、术后脑梗死、术后脑血管痉挛、术后脑积水和单线夹闭(动脉瘤夹夹片在一条虚拟线上)与预后不良相关(P<0.05)。多因素Logistic回归分析结果显示,Hunt-Hess分级≥Ⅲ级、术后脑梗死和术后脑积水与预后不良呈正相关(OR值分别为10.899、10.300、2.483、14.360,P<0.05)。多线夹闭(动脉瘤夹夹片在两条及以上虚拟线上)与预后不良呈负相关(OR=0.089,P<0.05)。结论Hunt-Hess分级≥Ⅲ级、术后脑梗死和术后脑积水是前交通动脉瘤显微手术患者预后不良的独立危险因素,多线夹闭是预后良好的一个保护因素。
