In our study, two experimental plantations, respectively, with 24 and 32 new clones of P tomentosa, were established in Weixian County, Hebei Province and Wuzhi County, Henan Province using a completely randomized blo...In our study, two experimental plantations, respectively, with 24 and 32 new clones of P tomentosa, were established in Weixian County, Hebei Province and Wuzhi County, Henan Province using a completely randomized block design. A comparative study was conducted on the continuous 5-year-old height and diameter at breast height （DBH） of new clones in the two plantations. As well, based on genetic correlation over the years of testing of these clones, a preliminary study of early selection was carried out. Results indicate that the growth traits of the new clones in Weixian were better than those in Wuzhi. The traits show weak correlation between the two plantations. In some stands, the height, DBH and seedling volume of 5-year-old clones presented statistically significant differences among clones. In both plantations, the new clones showed over 0.6 repeatability of height, DBH and volume, as well as larger coefficients of variation （CV）. The fact that these clones achieved the largest repeatability and CV in the second year suggests that these traits are highly controlled by heredity. Thus, based on the growth traits of the second year, the new clones B305, B307, B303, H75, BT18, BT17 and 21J-1 were considered suitable in Weixian. In Wuzhi, the new clones had variable repeatability and CVs in various years and their correlation of growth traits among different years was not high. We conclude that early selection of new clones was not feasible in Wuzhi.展开更多
Twenty-five new clones belong to Populus Aigeiros of both domestic and foreign origin hadbeen introduced and tested at nursery stage in Shandong province. Results showed that height (H),diameter at stem base (D0) and ...Twenty-five new clones belong to Populus Aigeiros of both domestic and foreign origin hadbeen introduced and tested at nursery stage in Shandong province. Results showed that height (H),diameter at stem base (D0) and survival rate (SR) varied significantly and genetic variation were very largeamong the clones. CVg and broad-sense heritability (h2) of H, D0 and SR of 1-year-old stock nursery were7.43%, 9.25%, 18.78% and 78.91%, 96.31%, 95.93%, respectively, showing high genetic control on thetested traits. 11 superior clones with characteristics of high growth rate and medium or high SR wereprimarily selected and genetic gains (△G) of H, D0 and SR were 16.89%, 16.08% and 13.08%, respectively.Rooting habits test of some selected clones were also conducted based on the cutting culture in watercontainer and annual growth increment measured. The date of first root emergence, number of main roots,number of lateral roots, length of main roots and the emergence date of growth peak varied to certain degreeamong the selected clones.展开更多
In the present study,AFP-specific immune RNA(iRNA) was shown to induceplaque-forming cells(PFC)from mouse splenocytes with a minimal effective dose of 31 μg/mland an optimal dose plateau ranging from 125 μg/ml to 2...In the present study,AFP-specific immune RNA(iRNA) was shown to induceplaque-forming cells(PFC)from mouse splenocytes with a minimal effective dose of 31 μg/mland an optimal dose plateau ranging from 125 μg/ml to 250 μg/ml.This action of iRNA may beobserved on the first day of in vitro culture of mouse spleen cells.The iRNA preparation wasproved sensitive to RNase,but not to DNase and pronase.In addition immunofluorescencetest revealed that AFP-specific iRNA is capable of inducing the expression of membranousanti-AFP antibodies or AFP receptors in mouse spleen cells.展开更多
Objective Genotypes(G)1,3,and 5 of the Japanese encephalitis virus(JEV)have been isolated in China,but the dominant genotype circulating in Chinese coastal areas remains unknown.We searched for G5 JEV-infected cases a...Objective Genotypes(G)1,3,and 5 of the Japanese encephalitis virus(JEV)have been isolated in China,but the dominant genotype circulating in Chinese coastal areas remains unknown.We searched for G5 JEV-infected cases and attempted to elucidate which JEV genotype was most closely related to human Japanese encephalitis(JE)in the coastal provinces of China.Methods In this study,we collected serum specimens from patients with JE in three coastal provinces of China(Guangdong,Zhejiang,and Shandong)from 2018 to 2020 and conducted JEV cross-neutralization tests against G1,G3,and G5.Results Acute serum specimens from clinically reported JE cases were obtained for laboratory confirmation from hospitals in Shandong(92 patients),Zhejiang(192 patients),and Guangdong(77 patients),China,from 2018 to 2020.Seventy of the 361 serum specimens were laboratory-confirmed to be infected with JEV.Two cases were confirmed to be infected with G1 JEV,32 with G3 JEV,and two with G5 JEV.Conclusion G3 was the primary infection genotype among JE cases with a definite infection genotype,and the infection caused by G5 JEV was confirmed serologically in China.展开更多
Thirty poplar clones of either domestic or foreign origin in Aigeiros section were introduced for regional planting test contrasting with I-69 (Populus deltoides ‘Lux’). Twelve experimental plots were planted separa...Thirty poplar clones of either domestic or foreign origin in Aigeiros section were introduced for regional planting test contrasting with I-69 (Populus deltoides ‘Lux’). Twelve experimental plots were planted separately in southwest, south, east, and northwest regions within Shandong Province. Combined variance analysis of multiple experimental sites showed that there were significant differences within sites and clones. The interaction between clone and environment was significant in the southwest region of Shandong Province, while those weren’t in other regions. With a analysis of multiple contrast (Duncan’s method), effective value of clone (C_i), coefficient of variation for interaction effect variance (_iCV) and regression coefficient (b_i), some superior clones with the characteristics of fast growth rate, higher genetic stability and growth adaptability, were screened out for each poplar cultivation region in Shandong Province.展开更多
基金the National Project of ScienceTechnology for the 11th Five-Year Plan (Grant No.2006BAD01A1502)
文摘In our study, two experimental plantations, respectively, with 24 and 32 new clones of P tomentosa, were established in Weixian County, Hebei Province and Wuzhi County, Henan Province using a completely randomized block design. A comparative study was conducted on the continuous 5-year-old height and diameter at breast height （DBH） of new clones in the two plantations. As well, based on genetic correlation over the years of testing of these clones, a preliminary study of early selection was carried out. Results indicate that the growth traits of the new clones in Weixian were better than those in Wuzhi. The traits show weak correlation between the two plantations. In some stands, the height, DBH and seedling volume of 5-year-old clones presented statistically significant differences among clones. In both plantations, the new clones showed over 0.6 repeatability of height, DBH and volume, as well as larger coefficients of variation （CV）. The fact that these clones achieved the largest repeatability and CV in the second year suggests that these traits are highly controlled by heredity. Thus, based on the growth traits of the second year, the new clones B305, B307, B303, H75, BT18, BT17 and 21J-1 were considered suitable in Weixian. In Wuzhi, the new clones had variable repeatability and CVs in various years and their correlation of growth traits among different years was not high. We conclude that early selection of new clones was not feasible in Wuzhi.
文摘Twenty-five new clones belong to Populus Aigeiros of both domestic and foreign origin hadbeen introduced and tested at nursery stage in Shandong province. Results showed that height (H),diameter at stem base (D0) and survival rate (SR) varied significantly and genetic variation were very largeamong the clones. CVg and broad-sense heritability (h2) of H, D0 and SR of 1-year-old stock nursery were7.43%, 9.25%, 18.78% and 78.91%, 96.31%, 95.93%, respectively, showing high genetic control on thetested traits. 11 superior clones with characteristics of high growth rate and medium or high SR wereprimarily selected and genetic gains (△G) of H, D0 and SR were 16.89%, 16.08% and 13.08%, respectively.Rooting habits test of some selected clones were also conducted based on the cutting culture in watercontainer and annual growth increment measured. The date of first root emergence, number of main roots,number of lateral roots, length of main roots and the emergence date of growth peak varied to certain degreeamong the selected clones.
文摘In the present study,AFP-specific immune RNA(iRNA) was shown to induceplaque-forming cells(PFC)from mouse splenocytes with a minimal effective dose of 31 μg/mland an optimal dose plateau ranging from 125 μg/ml to 250 μg/ml.This action of iRNA may beobserved on the first day of in vitro culture of mouse spleen cells.The iRNA preparation wasproved sensitive to RNase,but not to DNase and pronase.In addition immunofluorescencetest revealed that AFP-specific iRNA is capable of inducing the expression of membranousanti-AFP antibodies or AFP receptors in mouse spleen cells.
基金supported by the National Key Research and Development Program[2022YFC2302700].
文摘Objective Genotypes(G)1,3,and 5 of the Japanese encephalitis virus(JEV)have been isolated in China,but the dominant genotype circulating in Chinese coastal areas remains unknown.We searched for G5 JEV-infected cases and attempted to elucidate which JEV genotype was most closely related to human Japanese encephalitis(JE)in the coastal provinces of China.Methods In this study,we collected serum specimens from patients with JE in three coastal provinces of China(Guangdong,Zhejiang,and Shandong)from 2018 to 2020 and conducted JEV cross-neutralization tests against G1,G3,and G5.Results Acute serum specimens from clinically reported JE cases were obtained for laboratory confirmation from hospitals in Shandong(92 patients),Zhejiang(192 patients),and Guangdong(77 patients),China,from 2018 to 2020.Seventy of the 361 serum specimens were laboratory-confirmed to be infected with JEV.Two cases were confirmed to be infected with G1 JEV,32 with G3 JEV,and two with G5 JEV.Conclusion G3 was the primary infection genotype among JE cases with a definite infection genotype,and the infection caused by G5 JEV was confirmed serologically in China.
文摘目的:基于血常规和颈动脉斑块构建一种个性化nomogram风险预测模型预测颈动脉粥样硬化(carotid atherosclerosis, CAS)患者发生缺血性脑卒中(cerebral ischemic stroke, CIS)的风险。方法:选取2021年3月1日至2022年3月1日在上海市第八人民医院神经内科住院的CAS患者214例,收集患者的基本特征、血常规指标及影像学检查数据。根据是否发生缺血性脑卒中分别分为两组,随机抽取全部数据按7∶3的比例拆分为建模组和验证组。采用单因素logistic回归和lasso回归筛选CAS患者发生缺血性脑卒中的独立风险预测因子,将其导入R软件构建nomogram预测模型。ROC曲线下面积(AUC)、校准曲线和DCA决策曲线对模型进行内部验证。结果:单因素logistic回归和lasso回归分析结果显示,红细胞分布宽度、大型血小板比率、血小板计数是CAS患者发生缺血性脑卒中的独立风险预测因子(P<0.05),由于年龄对于CIS具有重要临床意义,最终也将其纳入模型。基于上述预测因子导入R软件构建nomogram预测模型并进行模型内部验证。建模组受试者工作特征曲线下面积(area under the curve, AUC)为0.644,验证组AUC为0.677,表示该nomogram模型预测能力较好。Hosmer-Lemeshow拟合优度检验(P=0.058),表明该模型具有较好的区分度。DCA曲线显示风险阈值为8%~45%时使用该模型具有临床实用价值。结论:本研究构建并验证了一个预测CAS患者发生缺血性脑卒中的nomogram风险预测模型,该模型预测能力和区分能力较好,对临床评估CAS患者发生缺血性脑卒中具有较高的临床实用价值。
文摘Thirty poplar clones of either domestic or foreign origin in Aigeiros section were introduced for regional planting test contrasting with I-69 (Populus deltoides ‘Lux’). Twelve experimental plots were planted separately in southwest, south, east, and northwest regions within Shandong Province. Combined variance analysis of multiple experimental sites showed that there were significant differences within sites and clones. The interaction between clone and environment was significant in the southwest region of Shandong Province, while those weren’t in other regions. With a analysis of multiple contrast (Duncan’s method), effective value of clone (C_i), coefficient of variation for interaction effect variance (_iCV) and regression coefficient (b_i), some superior clones with the characteristics of fast growth rate, higher genetic stability and growth adaptability, were screened out for each poplar cultivation region in Shandong Province.
