Rosa rugosa is an important garden ornamental plant which belongs to the genus Rosa of the family Rosaceae. The current wild and cultivated R. rugosa are mostly purple, pink, a small amount of white, but lack of yello...Rosa rugosa is an important garden ornamental plant which belongs to the genus Rosa of the family Rosaceae. The current wild and cultivated R. rugosa are mostly purple, pink, a small amount of white, but lack of yellow, orange and so on. Flavonoids 3’-hydroxylase belongs to CYP75B subfamily of cytochrome P450, and is an essential enzyme in anthocyanins synthesis. In this experiment, RrF3’H gene was cloned from the petal of Rosa rugosa ‘Hunchun’ using RT-PCR, and bioinformatics analysis was performed. The RrF3’H gene’s full length of opening reading frame was 1687 bp, encoding 510 amino acids. The formulas of proteins encoded by RrF3’H were C2666H4149N699O734S24. The derived protein had a molecular weight of 58,506.95 Da. The aliphatic index was 90.94. It belongs to unstable hydrophilic protein. The protein consists of 46.76% α-helix, 31.04% random coil, 7.66% β-corner and 14.54% extended strand. The protein contains 21 Ser phosphorylation sites, 12 Thr phosphorylation sites, and 2 Tyr phosphorylation sites. The protein contained two O-glycosylation sites, located at positions 98 and 263 of the amino acid sequence respectively. The protein has a signal peptide site and a transmembrane structure. In addition, by comparing the expression levels of RrF3’H, we found RrF3’H was positively correlated with the depth of flower color.展开更多
抗坏血酸(Vc)是重要的抗氧化剂,在茶树体内的各种生理代谢及抵御非生物胁迫中起重要作用,Vc含量关系到绿茶品质优劣。GDP-D-甘露糖焦磷酸化酶(GMP)是茶树Vc生物合成途径中的关键酶之一。本研究通过RACE-PCR方法从茶树中克隆了GMP c DNA...抗坏血酸(Vc)是重要的抗氧化剂,在茶树体内的各种生理代谢及抵御非生物胁迫中起重要作用,Vc含量关系到绿茶品质优劣。GDP-D-甘露糖焦磷酸化酶(GMP)是茶树Vc生物合成途径中的关键酶之一。本研究通过RACE-PCR方法从茶树中克隆了GMP c DNA全长序列,共计1510 bp,其中包含1 086 bp的开放阅读框(ORF),编码361个氨基酸,推测蛋白分子量为39.599 k Da。BLAST分析表明,茶树GMP基因氨基酸序列与猕猴桃的亲缘性最高,达到96%。实时定量PCR分析表明,茶树新梢芽下第三叶中GMP基因表达量最高,嫩茎中最低,不同品种茶树新梢叶片中表达量存在明显差异。高温胁迫初期,GMP基因表达量及抗坏血酸含量迅速升高,然后逐渐下降且均低于同期对照。展开更多
文摘Rosa rugosa is an important garden ornamental plant which belongs to the genus Rosa of the family Rosaceae. The current wild and cultivated R. rugosa are mostly purple, pink, a small amount of white, but lack of yellow, orange and so on. Flavonoids 3’-hydroxylase belongs to CYP75B subfamily of cytochrome P450, and is an essential enzyme in anthocyanins synthesis. In this experiment, RrF3’H gene was cloned from the petal of Rosa rugosa ‘Hunchun’ using RT-PCR, and bioinformatics analysis was performed. The RrF3’H gene’s full length of opening reading frame was 1687 bp, encoding 510 amino acids. The formulas of proteins encoded by RrF3’H were C2666H4149N699O734S24. The derived protein had a molecular weight of 58,506.95 Da. The aliphatic index was 90.94. It belongs to unstable hydrophilic protein. The protein consists of 46.76% α-helix, 31.04% random coil, 7.66% β-corner and 14.54% extended strand. The protein contains 21 Ser phosphorylation sites, 12 Thr phosphorylation sites, and 2 Tyr phosphorylation sites. The protein contained two O-glycosylation sites, located at positions 98 and 263 of the amino acid sequence respectively. The protein has a signal peptide site and a transmembrane structure. In addition, by comparing the expression levels of RrF3’H, we found RrF3’H was positively correlated with the depth of flower color.
文摘抗坏血酸(Vc)是重要的抗氧化剂,在茶树体内的各种生理代谢及抵御非生物胁迫中起重要作用,Vc含量关系到绿茶品质优劣。GDP-D-甘露糖焦磷酸化酶(GMP)是茶树Vc生物合成途径中的关键酶之一。本研究通过RACE-PCR方法从茶树中克隆了GMP c DNA全长序列,共计1510 bp,其中包含1 086 bp的开放阅读框(ORF),编码361个氨基酸,推测蛋白分子量为39.599 k Da。BLAST分析表明,茶树GMP基因氨基酸序列与猕猴桃的亲缘性最高,达到96%。实时定量PCR分析表明,茶树新梢芽下第三叶中GMP基因表达量最高,嫩茎中最低,不同品种茶树新梢叶片中表达量存在明显差异。高温胁迫初期,GMP基因表达量及抗坏血酸含量迅速升高,然后逐渐下降且均低于同期对照。
