The study established a method to analyze clorsulon concentration in sheep plasma byreverse phase-high performance liquid chromatography (RP-HPLC) with an internal-standard.In plasma, clorsulon concentration within 0....The study established a method to analyze clorsulon concentration in sheep plasma byreverse phase-high performance liquid chromatography (RP-HPLC) with an internal-standard.In plasma, clorsulon concentration within 0.01-1.0gmL-1 and 1.0-20gmL-1 ranges had agood linear relationship (R=0.9991, 0.9958). The average recovery of the method was 98.11±3.52%. The relative standard deviation (RSD)s% of within-day and between-day assayswere less than 7 and 8% respectively. After giving clorsulon to sheep by intravenous (i.v.)or subcutaneous (s.c.) routes at a single dose of 4mgkg-1, drug concentration-time datain plasma were both fitted to a two-compartment open model. The main pharmacokineticparameters were: 1) i.v. administration: T1/2β=10.040.819h, area under the concentrationversus time curve (AUC)=81.8514.24mghL-1; 2) s.c. administration: T1/2ka=1.360.75h,T=71.0314.15%. The results indicated that clorsulon in sheep following a single s.c.administration was absorbed rapidly and eliminated slower than that following a single i.v.administration, and showed a better bioavailability.展开更多
The aim of the present study was to assess the fasciolicidal and nematicidal efficacy of a new combination of clorsulon/levamisole, which was formulated for intramuscular administration, in dairy cattle. For the study...The aim of the present study was to assess the fasciolicidal and nematicidal efficacy of a new combination of clorsulon/levamisole, which was formulated for intramuscular administration, in dairy cattle. For the study, sixteen Holstein-Freisian dairy cows were selected for inclusion in the trial based on finding Fasciola hepatica eggs or gastrointestinal nematodes (GIN) in the feces. Ten of these cows were naturally infected with F. hepatica, and 6 were naturally infected with GIN. On Day 0 (zero), all animals were treated with a combination of clorsulon (2.5 mg/kg/IM and levamisole (2.5 mg/kg/IM) administered at the dose recommended by the manufacturer (1 ml/20kg/bw). Subsequently, their feces were analyzed on days 0, 7, 14 and 21 post treatments by the Flukefinder sedimentation method and McMaster technique to determine the percentages of egg reduction in the animals, with reference to the egg load count on day 0. The results obtained in the current work indicated an efficacy of 100% that was determined by the percentage reduction of eggs of F. hepatica and/or GIN. This new fasciolicide/nematicide formulation exerted a remarkable effect and can be considered a new alternative to control F. hepatica and gastrointestinal worms in dairy cattle.展开更多
文摘The study established a method to analyze clorsulon concentration in sheep plasma byreverse phase-high performance liquid chromatography (RP-HPLC) with an internal-standard.In plasma, clorsulon concentration within 0.01-1.0gmL-1 and 1.0-20gmL-1 ranges had agood linear relationship (R=0.9991, 0.9958). The average recovery of the method was 98.11±3.52%. The relative standard deviation (RSD)s% of within-day and between-day assayswere less than 7 and 8% respectively. After giving clorsulon to sheep by intravenous (i.v.)or subcutaneous (s.c.) routes at a single dose of 4mgkg-1, drug concentration-time datain plasma were both fitted to a two-compartment open model. The main pharmacokineticparameters were: 1) i.v. administration: T1/2β=10.040.819h, area under the concentrationversus time curve (AUC)=81.8514.24mghL-1; 2) s.c. administration: T1/2ka=1.360.75h,T=71.0314.15%. The results indicated that clorsulon in sheep following a single s.c.administration was absorbed rapidly and eliminated slower than that following a single i.v.administration, and showed a better bioavailability.
文摘The aim of the present study was to assess the fasciolicidal and nematicidal efficacy of a new combination of clorsulon/levamisole, which was formulated for intramuscular administration, in dairy cattle. For the study, sixteen Holstein-Freisian dairy cows were selected for inclusion in the trial based on finding Fasciola hepatica eggs or gastrointestinal nematodes (GIN) in the feces. Ten of these cows were naturally infected with F. hepatica, and 6 were naturally infected with GIN. On Day 0 (zero), all animals were treated with a combination of clorsulon (2.5 mg/kg/IM and levamisole (2.5 mg/kg/IM) administered at the dose recommended by the manufacturer (1 ml/20kg/bw). Subsequently, their feces were analyzed on days 0, 7, 14 and 21 post treatments by the Flukefinder sedimentation method and McMaster technique to determine the percentages of egg reduction in the animals, with reference to the egg load count on day 0. The results obtained in the current work indicated an efficacy of 100% that was determined by the percentage reduction of eggs of F. hepatica and/or GIN. This new fasciolicide/nematicide formulation exerted a remarkable effect and can be considered a new alternative to control F. hepatica and gastrointestinal worms in dairy cattle.
