Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regen...Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.展开更多
Sealed-off carbon dioxide lasers encounter problem of dissociation of CO2, Which causes the output power to fall. Reformation of CO2 is therefore essential for long life CO2 lasers. Au/Fe2O3 and Au/NiFe2O4 are promis...Sealed-off carbon dioxide lasers encounter problem of dissociation of CO2, Which causes the output power to fall. Reformation of CO2 is therefore essential for long life CO2 lasers. Au/Fe2O3 and Au/NiFe2O4 are promising candidates for this application.展开更多
Current use of enrichment and processing technologies of ores requires the introduction of closed circuits of water treatment. A decrease in technological properties is caused by accumulations of ion-molecular compone...Current use of enrichment and processing technologies of ores requires the introduction of closed circuits of water treatment. A decrease in technological properties is caused by accumulations of ion-molecular components in the circulating water. The objective of the simulation is to determine the maximum allowable concentrations of ions and molecules as well as the choice of conditions for deposition or adsorption.First of all, our examinations decrease the concentration of copper ions and fatty acids in the circulating water. By pre-mixing water with the highest concentration of these ions, a reduction of copper ion and fatty acid concentrations in the recycled water occurs. The results do not only ensure the achievement of the maximum permitted concentration(MPC) of copper and iron, significantly reducing the amount of oxidized copper, they also make it possible to use the united sewage as current water for the flotation process. Mixing and adding filtrate of tailings, discharges of urban wastewater treatment and effluent of ash pit of thermal power stations(TPS) to recycled water causes an increase in the capacity of the enrichment plant by 15–17%.展开更多
Studies were made of the symmetric tensile-compressive low cycle fatigue behaviour and the influence of dynamic strain aging(DSA)pre-treatment of 18-8 austenitic stainless steel. Within the testing amplitude range of ...Studies were made of the symmetric tensile-compressive low cycle fatigue behaviour and the influence of dynamic strain aging(DSA)pre-treatment of 18-8 austenitic stainless steel. Within the testing amplitude range of strain.±0.5 % to±1.5 %,the three processes of cyclic hardening,cyclic saturation and cyclic softening were observed.In the same amplitude of strain,the peak stress of the samples pre-treated by DSA is higher than that of solid-solu- tion and cold working pre-treatment,but no remarkable differences of the fatigue lives of them were found.TEM observation shows that the uniform and stable dislocation networks with high density form after DSA pre-treatment,which increases the cyclic peak stress.The cyclic softening results from the low dislocation density and elongated cell structure with low energy.展开更多
Objective:Chronic kidney disease(CKD)is a progressive disorder characterized by intricate structural and functional alterations in the kidneys,attributable to diverse causative factors.Notably,the therapeutic promise ...Objective:Chronic kidney disease(CKD)is a progressive disorder characterized by intricate structural and functional alterations in the kidneys,attributable to diverse causative factors.Notably,the therapeutic promise of miR-145-5p in addressing renal pathologies has been discerned.This investigation seeks to elucidate the functional role of miR-145-5p in injured kidneys by subjecting human glomerular mesangial cells(HGMCs)to stimulation with Angiotensin II(AngII).Materials and Methods:Cellular viability and the levels of inflammatory mediators were evaluated utilizing Cell Counting Kit-8(CCK-8),quantitative real-time polymerase chain reaction(qRT-PCR),and western blot methodologies,both in the presence of AngII incubation and in scenarios of miR-145p overexpression and downregulation.Furthermore,the cell cycle dynamics were elucidated through Fluorescence-activated Cell Sorting(FACS)analysis.Results:AngII incubation induced an upregulation of miR-145-5p and inflammatory factors including Intercellular Adhesion Molecule 1(ICAM-1),Interleukin 6(IL-6),Interleukin 8(IL-8),and Interleukin 1β(IL-1β).Additionally,it elevated the expression of Cyclin A2,Cyclin D1,and the G2/M cell cycle ratio.Conversely,inhibition of miR-145-5p heightened the levels of inflammatory factors and cell cycle regulators induced by AngII incubation.Reduced expression of miR-145-5p correlated with a downregulation of Interleukin 10(IL-10)expression,concurrently promoting HGMC proliferation under AngII stimulation.Moreover,ectopic miR-145-5p expression demonstrated a reduction in inflammatory factors,cell cyclin regulators,G2/M cell cycle ratio,and overall proliferation.Conclusion:MiR-145-5p exhibited inhibitory effects on the inflammatory response and proliferation induced by Angiotensin II in HGMCs,showcasing its potential as a therapeutic avenue for the treatment of kidney injury.展开更多
The wavelet transform method for high-quality time-frequency analysis is applied to sets of observations of relative sunspot numbers and stellar chromosphere fluxes of 10 Sun-like stars. Wavelet analysis of solar data...The wavelet transform method for high-quality time-frequency analysis is applied to sets of observations of relative sunspot numbers and stellar chromosphere fluxes of 10 Sun-like stars. Wavelet analysis of solar data shows that in a certain interval of time there are several cycles of activity with pe- riods of duration which vary considerably from each other: from quasi-biennial cycles to lO0-yr cycles. Cyclic activity was detected in almost all Sun-like stars that we examined, even those that previously were not considered as stars with cyclic activity according to analysis using a Scargle periodogram. The durations of solar and stellar cycles significantly change during the observation period.展开更多
The publication of ISO 15686-5 is significant for studies related to the life-cycle costs of buildings. Among the many economic evaluation methods, the NPV method is recommended for use by this standard. This stu...The publication of ISO 15686-5 is significant for studies related to the life-cycle costs of buildings. Among the many economic evaluation methods, the NPV method is recommended for use by this standard. This study explains the concept and method of PV, and then provides an explanation of the concept and method of NPV, and points out the limitations in the practical application of the method.展开更多
To investigate whether the Bcl- 2 gene family is involved in m odulating mechanism of apoptosis and change of cell cycle protein induced by curcumin in acute myeloid leukemia HL - 6 0 cell line and primary acute m y...To investigate whether the Bcl- 2 gene family is involved in m odulating mechanism of apoptosis and change of cell cycle protein induced by curcumin in acute myeloid leukemia HL - 6 0 cell line and primary acute m yelogenous leukem ic cells,the Bcl- 2 family member Mcl- 1,Bax and Bak and cell cycle proteins including P2 7kipl,P2 1wafl,cyclin D3and p Rbp- were selected and their ex- pression detected by SABC imm uno- histochem ical stain m ethod.The attitude of sub- G1 peak in DNA histogram was determined by FCM.The TU NEL positive cell percentage was identified by term inal deoxynucleotidyl transferase (Td T ) - m ediated Biotin d U NP end labeling technique.It was found that when HL - 6 0 cells were treated with 2 5μm ol/ L curcumin for 2 4 h,the expression level of Mcl- 1was down- regulated,but that of Bax and Bak up- regulated time- dependently.There was significant difference in the expression level of Mcl- 1,Bax and Bak between the curcumin- treated groups and control group(P<0 .0 5 - 0 .0 1) .At the sam e time,curcumin had no effect on progress of cell cycle in prim aty acute m yelogenous leukemia at newly diagnosis,but could in- crease the peak of Sub- G1 (P<0 .0 5 ) ,and down- regulate the expression of Mcl- 1and up- regulate the expression of Bax and Bak with the difference being statistically significant.The expression of P2 7kipl,P2 1wafl and p Rbp- were elevated and thatof cyclin D3decreased in the presence of curcum in. These findings suggested thatthe Bcl- 2 gene fam ily indeed participated in the regulatory process of apoptosis induced by curcumin in HL - 6 0 cells and AML cells.Curcumin can induce apoptosis of primary acute myelogenous leukemic cells and disturb cell cycle progression of HL - 6 0 cells.The m echanism appeared to be m ediated by perturbing G0 / G1 phases checkpoints which associated with up- regulation of P2 7kipl,P2 1wafl and p Rbp- expression,and down- regulation of cyclin D3.展开更多
With the use of the Royal Greenwich Observatory data set of sunspot groups, an attempt is made to examine the north-south asymmetry of solar activity in the 'extended' solar cycles. It is inferred that the asy...With the use of the Royal Greenwich Observatory data set of sunspot groups, an attempt is made to examine the north-south asymmetry of solar activity in the 'extended' solar cycles. It is inferred that the asymmetry established for individual solar cycles does not extend to the 'extended' cycles.展开更多
Novel small sulfur heterocyclic quinones(6a,16adihydrobenzo[b]naphtho[2′,3′:5,6][1,4]dithiino[2,3-i]thianthrene-5,7,9,14,16,18-hexaone(4S6Q)and benzo[b]naphtho[2′,3′:5,6][1,4]dithiino[2,3-i]thianthrene-5,9,14,18-t...Novel small sulfur heterocyclic quinones(6a,16adihydrobenzo[b]naphtho[2′,3′:5,6][1,4]dithiino[2,3-i]thianthrene-5,7,9,14,16,18-hexaone(4S6Q)and benzo[b]naphtho[2′,3′:5,6][1,4]dithiino[2,3-i]thianthrene-5,9,14,18-tetraone(4S4Q))are developed by molecule structural design method and as cathode for aqueous zincorganic batteries.The conjugated thioether(–S–)bonds as connected units not only improve the conductivity of compounds but also inhibit their dissolution by both extendedπ-conjugated plane and constructed flexible molecular skeleton.Hence,the Zn//4S6Q and Zn//4S4Q batteries exhibit satisfactory electrochemical performance based on 3.5 mol L-1(M)Zn(ClO4)2electrolyte.For instance,the Zn//4S6Q battery obtains 240 and 208.6 mAh g^(-1)of discharge capacity at 150 mA g^(-1)and 30 A g^(-1),respectively.The excellent rate capability is ascribed to the fast reaction kinetics.This system displays a superlong life of 20,000 cycles with no capacity fading at 3 A g^(-1).Additionally,the H+-storage mechanism of the 4S6Q compound is demonstrated by ex situ analyses and density functional theory calculations.Impressively,the battery can normally work at-60℃benefiting from the anti-freezing electrolyte and maintain a high discharge capacity of 201.7 mAh g^(-1),which is 86.2%of discharge capacity at 25℃.The cutting-edge electrochemical performances of these novel compounds make them alternative electrode materials for Zn-organic batteries.展开更多
To investigate the relationship between the expression of cyclooxygenase-2 (COX-2) and menstrual cycle, the regulatory effects of 17-β-estradiol (E2) and medroxyprogesterone acetate (MPA) on the expression of C...To investigate the relationship between the expression of cyclooxygenase-2 (COX-2) and menstrual cycle, the regulatory effects of 17-β-estradiol (E2) and medroxyprogesterone acetate (MPA) on the expression of COX-2 in cervical cancer Hela cells were examined. Cervical cancer specimens were obtained from 47 pre-menopausal patients. The phase of menstrual cycle was determined by case history and HE staining of uterine endometrium. COX-2 was immunohistochemically stained by SABC staining and the staining intensity was determined with computerized image analysis system. Hela cells were incubated with alcohol, E2, E2+MPA, MPA for 12, 24 and 48 h respectively. The expression of COX-2 in Hela cells was detected by Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Our results showed that the expression of COX-2 was significantly higher during proliferative phase than secretory phase (P〈0.05), but there was no difference in the positive rate between proliferative phase and secretory phase (P〉0.05). Incubation with E2 could significantly enhance the expression of COX-2 continually. On the contrary, E2+MPA and MPA alone could decrease the expression of COX-2 as compared with the control and E2 group (P〈0.05 and P〈0.01 respectively). It is concluded that the expression of COX-2 in cervical cancer of pre-menopausal patients and Hela cells was regulated by estrogen/progestogen.展开更多
Space nuclear reactor power(SNRP)using a gas-cooled reactor(GCR)and a closed Brayton cycle(CBC)is the ideal choice for future high-power space missions.To investigate the safety characteristics and develop the control...Space nuclear reactor power(SNRP)using a gas-cooled reactor(GCR)and a closed Brayton cycle(CBC)is the ideal choice for future high-power space missions.To investigate the safety characteristics and develop the control strategies for gas-cooled SNRP,transient models for GCR,energy conversion unit,pipes,heat exchangers,pump and heat pipe radiator are established and a system analysis code is developed in this paper.Then,analyses of several operation conditions are performed using this code.In full-power steady-state operation,the core hot spot of 1293 K occurs near the upper part of the core.If 0.4$reactivity is introduced into the core,the maximum temperature that the fuel can reach is 2059 K,which is 914 K lower than the fuel melting point.The system finally has the ability to achieve a new steady-state with a higher reactor power.When the GCR is shut down in an emergency,the residual heat of the reactor can be removed through the conduction of the core and radiation heat transfer.The results indicate that the designed GCR is inherently safe owing to its negative reactivity feedback and passive decay heat removal.This paper may provide valuable references for safety design and analysis of the gas-cooled SNRP coupled with CBC.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82271397(to MG),82001293(to MG),82171355(to RX),81971295(to RX)and 81671189(to RX)。
文摘Stem cell-based therapies have been proposed as a potential treatment for neural regeneration following closed head injury.We previously reported that induced neural stem cells exert beneficial effects on neural regeneration via cell replacement.However,the neural regeneration efficiency of induced neural stem cells remains limited.In this study,we explored differentially expressed genes and long non-coding RNAs to clarify the mechanism underlying the neurogenesis of induced neural stem cells.We found that H19 was the most downregulated neurogenesis-associated lnc RNA in induced neural stem cells compared with induced pluripotent stem cells.Additionally,we demonstrated that H19 levels in induced neural stem cells were markedly lower than those in induced pluripotent stem cells and were substantially higher than those in induced neural stem cell-derived neurons.We predicted the target genes of H19 and discovered that H19 directly interacts with mi R-325-3p,which directly interacts with Ctbp2 in induced pluripotent stem cells and induced neural stem cells.Silencing H19 or Ctbp2 impaired induced neural stem cell proliferation,and mi R-325-3p suppression restored the effect of H19 inhibition but not the effect of Ctbp2 inhibition.Furthermore,H19 silencing substantially promoted the neural differentiation of induced neural stem cells and did not induce apoptosis of induced neural stem cells.Notably,silencing H19 in induced neural stem cell grafts markedly accelerated the neurological recovery of closed head injury mice.Our results reveal that H19 regulates the neurogenesis of induced neural stem cells.H19 inhibition may promote the neural differentiation of induced neural stem cells,which is closely associated with neurological recovery following closed head injury.
文摘Sealed-off carbon dioxide lasers encounter problem of dissociation of CO2, Which causes the output power to fall. Reformation of CO2 is therefore essential for long life CO2 lasers. Au/Fe2O3 and Au/NiFe2O4 are promising candidates for this application.
文摘Current use of enrichment and processing technologies of ores requires the introduction of closed circuits of water treatment. A decrease in technological properties is caused by accumulations of ion-molecular components in the circulating water. The objective of the simulation is to determine the maximum allowable concentrations of ions and molecules as well as the choice of conditions for deposition or adsorption.First of all, our examinations decrease the concentration of copper ions and fatty acids in the circulating water. By pre-mixing water with the highest concentration of these ions, a reduction of copper ion and fatty acid concentrations in the recycled water occurs. The results do not only ensure the achievement of the maximum permitted concentration(MPC) of copper and iron, significantly reducing the amount of oxidized copper, they also make it possible to use the united sewage as current water for the flotation process. Mixing and adding filtrate of tailings, discharges of urban wastewater treatment and effluent of ash pit of thermal power stations(TPS) to recycled water causes an increase in the capacity of the enrichment plant by 15–17%.
文摘Studies were made of the symmetric tensile-compressive low cycle fatigue behaviour and the influence of dynamic strain aging(DSA)pre-treatment of 18-8 austenitic stainless steel. Within the testing amplitude range of strain.±0.5 % to±1.5 %,the three processes of cyclic hardening,cyclic saturation and cyclic softening were observed.In the same amplitude of strain,the peak stress of the samples pre-treated by DSA is higher than that of solid-solu- tion and cold working pre-treatment,but no remarkable differences of the fatigue lives of them were found.TEM observation shows that the uniform and stable dislocation networks with high density form after DSA pre-treatment,which increases the cyclic peak stress.The cyclic softening results from the low dislocation density and elongated cell structure with low energy.
基金This work was supported by Nantong Science and Technology Project(MS22022012,MS12021039,MS12018020,MS12018041,JC2020040)Jiangsu Provincial Laboratory Animal Association(DWXH202116)+1 种基金the Doctoral Scientific Research Foundation of Nantong University(135420505015,135422505037)National College Students’Innovation and Entrepreneurship Training Program(202110304036Z).
文摘Objective:Chronic kidney disease(CKD)is a progressive disorder characterized by intricate structural and functional alterations in the kidneys,attributable to diverse causative factors.Notably,the therapeutic promise of miR-145-5p in addressing renal pathologies has been discerned.This investigation seeks to elucidate the functional role of miR-145-5p in injured kidneys by subjecting human glomerular mesangial cells(HGMCs)to stimulation with Angiotensin II(AngII).Materials and Methods:Cellular viability and the levels of inflammatory mediators were evaluated utilizing Cell Counting Kit-8(CCK-8),quantitative real-time polymerase chain reaction(qRT-PCR),and western blot methodologies,both in the presence of AngII incubation and in scenarios of miR-145p overexpression and downregulation.Furthermore,the cell cycle dynamics were elucidated through Fluorescence-activated Cell Sorting(FACS)analysis.Results:AngII incubation induced an upregulation of miR-145-5p and inflammatory factors including Intercellular Adhesion Molecule 1(ICAM-1),Interleukin 6(IL-6),Interleukin 8(IL-8),and Interleukin 1β(IL-1β).Additionally,it elevated the expression of Cyclin A2,Cyclin D1,and the G2/M cell cycle ratio.Conversely,inhibition of miR-145-5p heightened the levels of inflammatory factors and cell cycle regulators induced by AngII incubation.Reduced expression of miR-145-5p correlated with a downregulation of Interleukin 10(IL-10)expression,concurrently promoting HGMC proliferation under AngII stimulation.Moreover,ectopic miR-145-5p expression demonstrated a reduction in inflammatory factors,cell cyclin regulators,G2/M cell cycle ratio,and overall proliferation.Conclusion:MiR-145-5p exhibited inhibitory effects on the inflammatory response and proliferation induced by Angiotensin II in HGMCs,showcasing its potential as a therapeutic avenue for the treatment of kidney injury.
文摘The wavelet transform method for high-quality time-frequency analysis is applied to sets of observations of relative sunspot numbers and stellar chromosphere fluxes of 10 Sun-like stars. Wavelet analysis of solar data shows that in a certain interval of time there are several cycles of activity with pe- riods of duration which vary considerably from each other: from quasi-biennial cycles to lO0-yr cycles. Cyclic activity was detected in almost all Sun-like stars that we examined, even those that previously were not considered as stars with cyclic activity according to analysis using a Scargle periodogram. The durations of solar and stellar cycles significantly change during the observation period.
文摘The publication of ISO 15686-5 is significant for studies related to the life-cycle costs of buildings. Among the many economic evaluation methods, the NPV method is recommended for use by this standard. This study explains the concept and method of PV, and then provides an explanation of the concept and method of NPV, and points out the limitations in the practical application of the method.
基金This project wassupport by a grantfrom National NaturalSciences Foundation ofChina(No. 39770 934)
文摘To investigate whether the Bcl- 2 gene family is involved in m odulating mechanism of apoptosis and change of cell cycle protein induced by curcumin in acute myeloid leukemia HL - 6 0 cell line and primary acute m yelogenous leukem ic cells,the Bcl- 2 family member Mcl- 1,Bax and Bak and cell cycle proteins including P2 7kipl,P2 1wafl,cyclin D3and p Rbp- were selected and their ex- pression detected by SABC imm uno- histochem ical stain m ethod.The attitude of sub- G1 peak in DNA histogram was determined by FCM.The TU NEL positive cell percentage was identified by term inal deoxynucleotidyl transferase (Td T ) - m ediated Biotin d U NP end labeling technique.It was found that when HL - 6 0 cells were treated with 2 5μm ol/ L curcumin for 2 4 h,the expression level of Mcl- 1was down- regulated,but that of Bax and Bak up- regulated time- dependently.There was significant difference in the expression level of Mcl- 1,Bax and Bak between the curcumin- treated groups and control group(P<0 .0 5 - 0 .0 1) .At the sam e time,curcumin had no effect on progress of cell cycle in prim aty acute m yelogenous leukemia at newly diagnosis,but could in- crease the peak of Sub- G1 (P<0 .0 5 ) ,and down- regulate the expression of Mcl- 1and up- regulate the expression of Bax and Bak with the difference being statistically significant.The expression of P2 7kipl,P2 1wafl and p Rbp- were elevated and thatof cyclin D3decreased in the presence of curcum in. These findings suggested thatthe Bcl- 2 gene fam ily indeed participated in the regulatory process of apoptosis induced by curcumin in HL - 6 0 cells and AML cells.Curcumin can induce apoptosis of primary acute myelogenous leukemic cells and disturb cell cycle progression of HL - 6 0 cells.The m echanism appeared to be m ediated by perturbing G0 / G1 phases checkpoints which associated with up- regulation of P2 7kipl,P2 1wafl and p Rbp- expression,and down- regulation of cyclin D3.
基金the 973 project (G2000078408), theNational Science FOundations of China (10073019 and l9976301), the Science FOundation ofYu
文摘With the use of the Royal Greenwich Observatory data set of sunspot groups, an attempt is made to examine the north-south asymmetry of solar activity in the 'extended' solar cycles. It is inferred that the asymmetry established for individual solar cycles does not extend to the 'extended' cycles.
基金the National Natural Science Foundation of China(22279063 and 21835004)the National Key R&D Program of China(2016YFB0901500)+1 种基金Ministry of Education of China(B12015 and IRT13R30)the Haihe Laboratory of Sustainable Chemical Transformations for financial support。
文摘Novel small sulfur heterocyclic quinones(6a,16adihydrobenzo[b]naphtho[2′,3′:5,6][1,4]dithiino[2,3-i]thianthrene-5,7,9,14,16,18-hexaone(4S6Q)and benzo[b]naphtho[2′,3′:5,6][1,4]dithiino[2,3-i]thianthrene-5,9,14,18-tetraone(4S4Q))are developed by molecule structural design method and as cathode for aqueous zincorganic batteries.The conjugated thioether(–S–)bonds as connected units not only improve the conductivity of compounds but also inhibit their dissolution by both extendedπ-conjugated plane and constructed flexible molecular skeleton.Hence,the Zn//4S6Q and Zn//4S4Q batteries exhibit satisfactory electrochemical performance based on 3.5 mol L-1(M)Zn(ClO4)2electrolyte.For instance,the Zn//4S6Q battery obtains 240 and 208.6 mAh g^(-1)of discharge capacity at 150 mA g^(-1)and 30 A g^(-1),respectively.The excellent rate capability is ascribed to the fast reaction kinetics.This system displays a superlong life of 20,000 cycles with no capacity fading at 3 A g^(-1).Additionally,the H+-storage mechanism of the 4S6Q compound is demonstrated by ex situ analyses and density functional theory calculations.Impressively,the battery can normally work at-60℃benefiting from the anti-freezing electrolyte and maintain a high discharge capacity of 201.7 mAh g^(-1),which is 86.2%of discharge capacity at 25℃.The cutting-edge electrochemical performances of these novel compounds make them alternative electrode materials for Zn-organic batteries.
文摘To investigate the relationship between the expression of cyclooxygenase-2 (COX-2) and menstrual cycle, the regulatory effects of 17-β-estradiol (E2) and medroxyprogesterone acetate (MPA) on the expression of COX-2 in cervical cancer Hela cells were examined. Cervical cancer specimens were obtained from 47 pre-menopausal patients. The phase of menstrual cycle was determined by case history and HE staining of uterine endometrium. COX-2 was immunohistochemically stained by SABC staining and the staining intensity was determined with computerized image analysis system. Hela cells were incubated with alcohol, E2, E2+MPA, MPA for 12, 24 and 48 h respectively. The expression of COX-2 in Hela cells was detected by Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Our results showed that the expression of COX-2 was significantly higher during proliferative phase than secretory phase (P〈0.05), but there was no difference in the positive rate between proliferative phase and secretory phase (P〉0.05). Incubation with E2 could significantly enhance the expression of COX-2 continually. On the contrary, E2+MPA and MPA alone could decrease the expression of COX-2 as compared with the control and E2 group (P〈0.05 and P〈0.01 respectively). It is concluded that the expression of COX-2 in cervical cancer of pre-menopausal patients and Hela cells was regulated by estrogen/progestogen.
基金the National Natural Science Foundation of China(Grant No.U1967203)the National Key R&D Program of China(Grant No.2019YFB1901100)and China Postdoctoral Science Foundation(Grant No.2019M3737).
文摘Space nuclear reactor power(SNRP)using a gas-cooled reactor(GCR)and a closed Brayton cycle(CBC)is the ideal choice for future high-power space missions.To investigate the safety characteristics and develop the control strategies for gas-cooled SNRP,transient models for GCR,energy conversion unit,pipes,heat exchangers,pump and heat pipe radiator are established and a system analysis code is developed in this paper.Then,analyses of several operation conditions are performed using this code.In full-power steady-state operation,the core hot spot of 1293 K occurs near the upper part of the core.If 0.4$reactivity is introduced into the core,the maximum temperature that the fuel can reach is 2059 K,which is 914 K lower than the fuel melting point.The system finally has the ability to achieve a new steady-state with a higher reactor power.When the GCR is shut down in an emergency,the residual heat of the reactor can be removed through the conduction of the core and radiation heat transfer.The results indicate that the designed GCR is inherently safe owing to its negative reactivity feedback and passive decay heat removal.This paper may provide valuable references for safety design and analysis of the gas-cooled SNRP coupled with CBC.