Huangdi's Internal Classics(Neijin) is one of the most important ancient medical classics, which plays far-reaching influence in medical field. More and more domestic and overseas scholars published their translat...Huangdi's Internal Classics(Neijin) is one of the most important ancient medical classics, which plays far-reaching influence in medical field. More and more domestic and overseas scholars published their translated texts on Neijing. Due to the diversity of editions and different understanding, the translating styles and contents are widely different. This study will focus on the different translating styles on culture-specific lexicon、figure of speech and four-Chinese-character structures in Neijin.展开更多
BACKGROUND: Choosing proper donor cells is one of keys in experimental and clinical studies on cell replacement therapy (CRT) for treating Parkinson disease (PD). Embryonic mesencephalic precursor cells (MPCs) ...BACKGROUND: Choosing proper donor cells is one of keys in experimental and clinical studies on cell replacement therapy (CRT) for treating Parkinson disease (PD). Embryonic mesencephalic precursor cells (MPCs) can stably differentiate into dopaminergic neuron after in vitro proliferated culture. As compared with embryonic stem cell and neural stem cell strains, cell composition of embryonic MPCs after primary culture is also the most close to that of embryonic mesencephalic ventral cell suspension without proliferated culture. Successful experience accumulated in the latter suggests that primary cultured embryonic MPCs might be the most potential donor cells in clinical application with CRT for treating PD so far. OBJECTIVE: To investigate the feasibility of primary cultured embryonic precursor cells cultured primarily as donor cells in CRT for treating PD in rats. DESIGN : A randomized and controlled trial taking SD rats as experimental animals.SETTING: Department of Neurosurgery, Huashan Hospital Affiliated to Fudan University.MATERIALS: This experiment was carried out at the Institute of Neuroscience, Shanghai Institute for Biological Science, Chinese Academy of Sciences from July 2003 to June 2004. Totally 26 female SD rats, with body mass of 200 to 220 g, were provided by Shanghai Experimental Animal Center of Chinese Academy of Sciences. METHODS : Stereotaxic injection of 6-hydroxydopamine into the medial forebrain bundle were perfored to develop PD model rat. Among 26 SD rats, 20 rats achieved a more than 5 turns/min in apomorphine induced rotation test, reaching the standard of PD model rats. Immunohistochemical detection was performed on 1 out of 20 model rats after execution, and the other 19 rats were randomly divided into control group (n=5), sham transplantation group (n=5)and cell grafted group (n=9). Primary cultured E12 MPC cell suspension (1.2×10^11 L^-1)were used as donor cells. 4μL primary cultured E12 MPC cell suspension prepared freshly was injected into the lesioned corpus striatum of rats in cell grafted group, and 4μL D-Hank's solution was injected in sham transplantation group in the same way. There was no injection in control group. Apomorphine-induced rotation rate of PD rats were recorded respectively in cell grafted group and sham transplantation group pre-operation (initial value) and at postoperative 2, 4, 6 and 16 weeks. Apomorphine-induced rotation rate of PD rats was recorded in control group at postoperative 2 months (initial value) and following 2,4,6 and 16 weeks. To determine TH antigen with immunohistological ABC method (DAB developing) at 6 months post-transplantation to investigate the differentiation and survival of donor cells in the host body.MAIN OUTCOME MEASURES: Apomorphine-induced rotation behavior before and after transplantation and the survival and differentiation of implanted cells in the host body at 6 months post-transplantation. RESULTS: Among 19 model rats, one rat died after transplantation respectively in the cell grafted group and sham transplantation group; finally 17 model rats entered the stage of result analysis. Relative apomorphine-induced rotation rate was significantly decreased in the cell grafted group as compared with that before transplantation , with significant difference (P 〈 0.01 .P 〈 0.05);the mean value of relative apomorphine-induced rotation rate was significantly decreased at postoperative 16 weeks in cell grafted group as compared with that of corresponding relative rotation rate in control group , also with significant difference (P 〈 0.05).Immunohistological results showed that donor cells could differentiate into large and multi-polar dopaminergic neurons in the host body. CONCLUSION : Primary cultured embryonic MPCs can be used as the donor cells in CRT for treating PD.展开更多
Idiom,the cream of the language,is a fixed group of words or a single word,or even a sentence with a special mean ing that cannot be guessed from the literal meaning of its components.In the long history,animals and h...Idiom,the cream of the language,is a fixed group of words or a single word,or even a sentence with a special mean ing that cannot be guessed from the literal meaning of its components.In the long history,animals and human beings coexist on the earth,interact on each other and depend on each other.While animals are closely linked to the life of human beings in the whole history,gradually,people are inclined to associate some animals with certain qualities or characteristics with human beings also with the same features,then,a number of animal idioms get into human's language.In this thesis,the author will accomplish the purpose by finding out the duality of human evolution,more animal idioms and analyze the reasons for the different figura tive images such as living environment,religions and customs.展开更多
The modern value of salt culture was analyzed firstly, and then the relationship between salt culture and sports leisure tourism landscape design was discussed. Afterwards, the cultural elements of sports leisure tour...The modern value of salt culture was analyzed firstly, and then the relationship between salt culture and sports leisure tourism landscape design was discussed. Afterwards, the cultural elements of sports leisure tourism landscape in salt culture were analyzed, and the examples of applying salt culture to sports leisure tourism landscape design were enumerated to explain how to spread salt culture through sports leisure tourism landscape.展开更多
OVER 180 years ago, a craftsman named Zhang Mingshan came to prominence in north China's Tian- jin for his colorful clay figurines. Zhang's work became so celebrated that he gained favor from the royal fam- ily of t...OVER 180 years ago, a craftsman named Zhang Mingshan came to prominence in north China's Tian- jin for his colorful clay figurines. Zhang's work became so celebrated that he gained favor from the royal fam- ily of the Qing Dynasty (1644-1911). who decided to decorate the royal palace with his work. At the time, the best craftsmen in the country were sponsored by the stateand separated from the business world, so throughout his life most of Zhang's pieces went to the palace or to friends; few were sold for profit.展开更多
CONFUCIUS, one of the most influential philosophers in Chinese history, is a house-hold name all over the modern world. His thought has exerted a far-reaching influence on generations of Chinese in almost all
文摘Huangdi's Internal Classics(Neijin) is one of the most important ancient medical classics, which plays far-reaching influence in medical field. More and more domestic and overseas scholars published their translated texts on Neijing. Due to the diversity of editions and different understanding, the translating styles and contents are widely different. This study will focus on the different translating styles on culture-specific lexicon、figure of speech and four-Chinese-character structures in Neijin.
文摘BACKGROUND: Choosing proper donor cells is one of keys in experimental and clinical studies on cell replacement therapy (CRT) for treating Parkinson disease (PD). Embryonic mesencephalic precursor cells (MPCs) can stably differentiate into dopaminergic neuron after in vitro proliferated culture. As compared with embryonic stem cell and neural stem cell strains, cell composition of embryonic MPCs after primary culture is also the most close to that of embryonic mesencephalic ventral cell suspension without proliferated culture. Successful experience accumulated in the latter suggests that primary cultured embryonic MPCs might be the most potential donor cells in clinical application with CRT for treating PD so far. OBJECTIVE: To investigate the feasibility of primary cultured embryonic precursor cells cultured primarily as donor cells in CRT for treating PD in rats. DESIGN : A randomized and controlled trial taking SD rats as experimental animals.SETTING: Department of Neurosurgery, Huashan Hospital Affiliated to Fudan University.MATERIALS: This experiment was carried out at the Institute of Neuroscience, Shanghai Institute for Biological Science, Chinese Academy of Sciences from July 2003 to June 2004. Totally 26 female SD rats, with body mass of 200 to 220 g, were provided by Shanghai Experimental Animal Center of Chinese Academy of Sciences. METHODS : Stereotaxic injection of 6-hydroxydopamine into the medial forebrain bundle were perfored to develop PD model rat. Among 26 SD rats, 20 rats achieved a more than 5 turns/min in apomorphine induced rotation test, reaching the standard of PD model rats. Immunohistochemical detection was performed on 1 out of 20 model rats after execution, and the other 19 rats were randomly divided into control group (n=5), sham transplantation group (n=5)and cell grafted group (n=9). Primary cultured E12 MPC cell suspension (1.2×10^11 L^-1)were used as donor cells. 4μL primary cultured E12 MPC cell suspension prepared freshly was injected into the lesioned corpus striatum of rats in cell grafted group, and 4μL D-Hank's solution was injected in sham transplantation group in the same way. There was no injection in control group. Apomorphine-induced rotation rate of PD rats were recorded respectively in cell grafted group and sham transplantation group pre-operation (initial value) and at postoperative 2, 4, 6 and 16 weeks. Apomorphine-induced rotation rate of PD rats was recorded in control group at postoperative 2 months (initial value) and following 2,4,6 and 16 weeks. To determine TH antigen with immunohistological ABC method (DAB developing) at 6 months post-transplantation to investigate the differentiation and survival of donor cells in the host body.MAIN OUTCOME MEASURES: Apomorphine-induced rotation behavior before and after transplantation and the survival and differentiation of implanted cells in the host body at 6 months post-transplantation. RESULTS: Among 19 model rats, one rat died after transplantation respectively in the cell grafted group and sham transplantation group; finally 17 model rats entered the stage of result analysis. Relative apomorphine-induced rotation rate was significantly decreased in the cell grafted group as compared with that before transplantation , with significant difference (P 〈 0.01 .P 〈 0.05);the mean value of relative apomorphine-induced rotation rate was significantly decreased at postoperative 16 weeks in cell grafted group as compared with that of corresponding relative rotation rate in control group , also with significant difference (P 〈 0.05).Immunohistological results showed that donor cells could differentiate into large and multi-polar dopaminergic neurons in the host body. CONCLUSION : Primary cultured embryonic MPCs can be used as the donor cells in CRT for treating PD.
文摘Idiom,the cream of the language,is a fixed group of words or a single word,or even a sentence with a special mean ing that cannot be guessed from the literal meaning of its components.In the long history,animals and human beings coexist on the earth,interact on each other and depend on each other.While animals are closely linked to the life of human beings in the whole history,gradually,people are inclined to associate some animals with certain qualities or characteristics with human beings also with the same features,then,a number of animal idioms get into human's language.In this thesis,the author will accomplish the purpose by finding out the duality of human evolution,more animal idioms and analyze the reasons for the different figura tive images such as living environment,religions and customs.
基金Sponsored by the Project of the Key Research Base of Humanities and Social Sciences in Sichuan Province-China Salt Culture Research Center,Sichuan University of Science&Engineering(YWHY18-04)(Salt Culture Research)Project Funded by Sichuan Provincial Humanities and Social Sciences Key Research Base(YWHY17-09)
文摘The modern value of salt culture was analyzed firstly, and then the relationship between salt culture and sports leisure tourism landscape design was discussed. Afterwards, the cultural elements of sports leisure tourism landscape in salt culture were analyzed, and the examples of applying salt culture to sports leisure tourism landscape design were enumerated to explain how to spread salt culture through sports leisure tourism landscape.
文摘OVER 180 years ago, a craftsman named Zhang Mingshan came to prominence in north China's Tian- jin for his colorful clay figurines. Zhang's work became so celebrated that he gained favor from the royal fam- ily of the Qing Dynasty (1644-1911). who decided to decorate the royal palace with his work. At the time, the best craftsmen in the country were sponsored by the stateand separated from the business world, so throughout his life most of Zhang's pieces went to the palace or to friends; few were sold for profit.
文摘CONFUCIUS, one of the most influential philosophers in Chinese history, is a house-hold name all over the modern world. His thought has exerted a far-reaching influence on generations of Chinese in almost all