Objective:To investigate the interacting effects of co-administration of methanol leaf extract of Catharanthus roseus（C.roseus） on the hypoglycemic activity of metformin as well as glibenclamide using experimental r...Objective:To investigate the interacting effects of co-administration of methanol leaf extract of Catharanthus roseus（C.roseus） on the hypoglycemic activity of metformin as well as glibenclamide using experimental rats.Methods:Phytochemical analysis as well as acute toxicity and lethality(LD<sub>50</sub>) test were carried out on its methanol leaf extract.The alloxan model for experimental induction of diabetes in rats was employed.Six groups comprising five rats each were used.GroupsⅡ,ⅢandⅣreceived 250 mg/kg of extract,100 mg/kg of metformin and 1 mg/kg of glibenclamide respectively,while V and VI were administered metformin-extract and glibenclamide-extract combinations respectively at doses as above.Group I served as negative control and received only distilled water.All administration was done once daily for seven days. Fasting blood glucose was determined at 2,12,24,72 and 168 h using a glucometer.One-way ANOVA with post-hoc tests was used to assess for significant difference due to administration of drug alone and with co-administration of drug and extract.Results:The LD<sub>50</sub> was 2 121.32 mg/kg. The phytochemical studies indicated the presence of saponins,tannins,alkaloids,phlotatannins, flavonoids,triterpenoids,reducing sugars,anthraquinones and glycosides.All medicaments significantly reduced blood glucose levels when compared with control alone（P【0.05） with the highest percentage reduction in blood glucose（64.86%） exhibited by metformin-extract combination.Conclusions:The leaf extract of C.roseus significandy increases the hypoglycemic effect of metformin.展开更多
血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选...血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选应急通用桥接复苏液,已发展成为大趋势。我们对全血在严重失血患者的应用历史和现状做一介绍,并提出我国采用LTOWB作为首选应急通用血液的进一步论证和验证研究建议。展开更多
文摘Objective:To investigate the interacting effects of co-administration of methanol leaf extract of Catharanthus roseus（C.roseus） on the hypoglycemic activity of metformin as well as glibenclamide using experimental rats.Methods:Phytochemical analysis as well as acute toxicity and lethality(LD<sub>50</sub>) test were carried out on its methanol leaf extract.The alloxan model for experimental induction of diabetes in rats was employed.Six groups comprising five rats each were used.GroupsⅡ,ⅢandⅣreceived 250 mg/kg of extract,100 mg/kg of metformin and 1 mg/kg of glibenclamide respectively,while V and VI were administered metformin-extract and glibenclamide-extract combinations respectively at doses as above.Group I served as negative control and received only distilled water.All administration was done once daily for seven days. Fasting blood glucose was determined at 2,12,24,72 and 168 h using a glucometer.One-way ANOVA with post-hoc tests was used to assess for significant difference due to administration of drug alone and with co-administration of drug and extract.Results:The LD<sub>50</sub> was 2 121.32 mg/kg. The phytochemical studies indicated the presence of saponins,tannins,alkaloids,phlotatannins, flavonoids,triterpenoids,reducing sugars,anthraquinones and glycosides.All medicaments significantly reduced blood glucose levels when compared with control alone（P【0.05） with the highest percentage reduction in blood glucose（64.86%） exhibited by metformin-extract combination.Conclusions:The leaf extract of C.roseus significandy increases the hypoglycemic effect of metformin.
文摘血液复苏是严重失血患者的关键急救措施。血液复苏液体的选用,经历了从全血到晶体液/胶体液、晶体液联合红细胞、等比例血液成分的演进过程。近20年来,采用低效价抗体O型全血(low titer group O whole blood,LTOWB)作为血液复苏的首选应急通用桥接复苏液,已发展成为大趋势。我们对全血在严重失血患者的应用历史和现状做一介绍,并提出我国采用LTOWB作为首选应急通用血液的进一步论证和验证研究建议。
