Microbial diversity in the saline-alkali soil of a coastal Tamarix chinensis woodland at Bohai Bay, China

Soil salinization or alkalization is a form of soil desertification. Coastal saline-alkali soil represents a type of desert and a key system in the network of ecosystems at the continent-ocean interface. Tamarix chinensis is a drought-tolerant plant that is widely distributed in the coastal saline-alkali soil of Bohai Bay, China. In this study, we used 454 pyrosequencing techniques to investigate the characteristics and distribution of the microbial diversity in coastal saline-alkali soil of the T. chinensis woodland at Bohai Bay. A total of 20,315 sequences were obtained, representing 19 known bacterial phyla and a large proportion of unclassified bacteria at the phylum level. Proteobacteria, Acidobacteria and Actinobacteria were the predominant phyla. The coverage of T. chinensis affected the microbial composition. At the phylum level, the relative abundance of y-Proteobacteria and Bacteroidetes decreased whereas Actinobacteria increased with the increasing coverage of T. chinensis. At the genus level, the proportions of Steroidobacter, Lechevalieria, Gp3 and Gp4 decreased with the increase of the vegetation coverage whereas the proportion of Nocardioides increased. A cluster analysis showed that the existing T. chinensis changed the niches for the microorganisms in the coastal saline-alkali soil, which caused changes in the microbial community. The analysis also distinguished the microbial community structure of the marginal area from those of the dense area and sparse area. Furthermore, the results also indicated that the distance to the seashore line could also affect certain groups of soil bacteria in this coastal saline-alkali soil, such as the family Cryomorphaceae and class Flavobacteria, whose population decreased as the distance increased. In addition, the seawater and temperature could be the driving factors that affected the changes.

Straw interlayer improves sunflower root growth:Evidence from moisture and salt migration and the microbial community in saline-alkali soil

A straw interlayer added to soil can effectively reduce soil salinity effects on plant growth,however,the effects of soil moisture,salt and microbial community composition on plant growth under a straw interlayer are unclear.A rhizobox study was conducted to investigate the role of straw interlayer thickness on soil moisture,salt migration,microbial community composition,as well as root growth in sunflower.The study included four treatments:Control(no straw interlayer);S3(straw interlayer of 3.0 cm);S5(straw interlayer of 5.0 cm);S7(straw interlayer of 7.0 cm).Straw interlayer treatments increased soil moisture by 8.2–11.0%after irrigation and decreased soil salt content after the bud stage in 0–40 cm soil.Total root length,total root surface area,average root diameter,total root volume and the number of root tips of sunflower plants were higher under straw interlayer treatments than in the control,and were the highest under the S5 treatment.This stimulated root growth was ascribed to the higher abundance of Chloroflexi and Verrucomicrobia bacteria in soil with a straw interlayer,which was increased by 55.7 and 54.7%,respectively,in the S5 treatment.Addition of a straw interlayer of 5 cm thickness is a practical and environmentally feasible approach for improving sunflower root growth in saline-alkali soil.

Effects of Vegetation Restoration Age on Soil C:N:P Stoichiometry in Yellow River Delta Coastal Wetland of China

Vegetation restoration can alter carbon(C),nitrogen(N),and phosphorus(P)cycles in coastal wetlands affecting C:N:P stoichiometry.However,the effects of restoration age on soil C:N:P stoichiometry are unclear.In this study,we examined the re-sponses of soil C,N,and P contents and their stoichiometric ratios to vegetation restoration age,focusing on below-ground processes and their relationships to aboveground vegetation community characteristics.We conducted an analysis of temporal gradients based on the'space for time'method to synthesize the effects of restoration age on soil C:N:P stoichiometry in the Yellow River Delta wetland of China.The findings suggest that the combined effects of restoration age and soil depth create complex patterns of shifting soil C:N:P stoichiometry.Specifically,restoration age significantly increased all topsoil C:N:P stoichiometries,except for soil total phosphorus(TP)and the C:N ratio,and slightly affected subsoil C:N:P stoichiometry.The effects of restoration age on the soil C:N ratio was well constrained owing to the coupled relationship between soil organic carbon(SOC)and total nitrogen(TN)contents,while soil TP con-tent was closely related to changes in plant species diversity.Importantly,we found that the topsoil C:N:P stoichiometry was signific-antly affected by plant species diversity,whereas the subsoil C:N:P stoichiometry was more easily regulated by pH and electric con-ductivity(EC).Overall,this study shows that vegetation restoration age elevated SOC and N contents and alleviated N limitation,which is useful for further assessing soil C:N:P stoichiometry in coastal restoration wetlands.

Effects of Microbial Fertilizers on Soil Improvement and Wheat Growth Characteristics in Saline-alkali Land

[Objectives]This study was conducted to further enrich the research on saline-alkali land improvement,and explore the effects of biological bacterial fertilizers containing Bacillus subtilis and Bacillus velezensis HM-3 in saline-alkali land improvement and crop growth promotion.[Methods]Wheat was planted in saline-alkali land in Huanghua City,Hebei Province,and a mixed application experiment was carried out using biological agents from Hemiao Biotechnology Co.,Ltd.[Results]Compared with the field of control check(CK),water-soluble salts and pH value in the experimental fields decreased,and living bacteria count in the soil increased.Meanwhile,the economic characters of wheat in the experimental fields showed excellent performance,with yields increasing by 39.09%and 27.49%compared with the CK.It could be seen that the application of biological bacterial fertilizers achieved obvious effects of improving saline-alkali soil and increasing wheat yield.[Conclusions]In this study,the effects of biological bacterial fertilizers on saline-alkali land and wheat growth characters were clarified,providing some technical support and theoretical guidance for wheat planting in Huanghua saline-alkali land.

Study on the Landscaping Technologies for Coastal Saline-Alkali Land in Tanghai County of Tangshan City

Tanghai County is one of the counties in Tangshan City with the highest saline-alkali degree, with 80% of its land being covered by saline-alkali soils. Through studying landscaping technologies for coastal saline-alkali land in Tanghai County, 3 dominant modes for landscaping engineering in such land were summarized as shallow underground pipe desalination technology, banding soil replacement technology and hole membrane soil replacement technology, which are different in application scope and also in cost (respectively 260, 210 and 170 yuan). 3 landscaping measures were also proposed, including promotion of suitable plant cultivation technologies, application of saline-alkali tolerant plants and biological improvement of soils. To explore economic and practical planting patterns for saline-alkali land, low-cost landscaping tests were conducted from the perspectives of improving landscaping engineering mode and optimizing landscaping measures, and the results showed that it was practical to apply low-cost landscaping patterns, and comprehensive ecological measures should be adopted to realize the sustained utilization of soil.

Effect of Mirabilis jalapa (Linn.) Growth on Microbial Community in Bioremediation of Petroleum-contaminated Saline-alkali Soil

Microbial biomass and species in the rhizosphere soil of Mirabilis jalapa（Linn.）（the saline-alkali soil contaminated by total petroleum hydrocarbon（TPH））were studied with the technology of phospholipid fatty acids（PLFAs） analysis,to explore the effects of Mirabilis jalapa（Linn.） growth on the structure characteristics of microbial communities and degradation of TPH in the petroleum-contaminated salinealkali soil.The result showed that compared with the CK soil without Mirabilis jalapa（Linn.）,the kind change rates of PLFAs were 71.4%,69.2% and 33.3% in spring,summer and autumn,respectively,and the degradation of TPH increased by 47.6%,28.3%,and 18.9% in the rhizosphere soil in spring,summer and autumn,respectively.Correlation analysis was used to determine the correlation between the degradation of TPH and the soil microbial communities：77.8% of the microbial PLFAs showed positive correlation（the correlation coefficient r﹥0） with the degradation of TPH,and 55.6% of the PLFAs had high positive correlation with the degradation of TPH with a correlation coefficient r ≥0.8.In addition,the relative contents of SAT and MONO had high correlation with the degradation of TPH in the CK soil,and the correlation coefficients were 0.92 and 0.60,respectively;but in the rhizosphere soil,42.1% of the PLFAs had positive correlation with it,and only21.1% had high positive correlation with the degradation of TPH,the relative contents of TBSAT,MONO and CYCLO had moderate or low positive correlation with the degradation of TPH,and the correlation coefficients were 0.56,0.50 and 0.07 respectively.It was shown that the growth of mirabilis jalapa（Linn.） highly affected the microbial community structure and TPH degradation speed in the rhizosphere soil,providing a theoretical basis for the research on phytoremediation of petroleumcontaminated saline-alkali soil.

Present situation and tendency of saline-alkali soil in west Jilin Province

Taking west Jilin Province as an example, this paper put forward the assessment index of salinization, and based on it, the authors present the distribution characteristics of saline-alkali soil in the 1980s and the 1990s in west Jilin and analyze its physical and chemical properties in detail. The developing tendency of salinization was also inferred by comparing the saline-alkali soil of the 1980s with that of the 1990s. Finally, the natural and human factors leading to salinization are analyzed.

Effects of combined drip irrigation and sub-surface pipe drainage on water and salt transport of saline-alkali soil in Xinjiang, China

Developing effective irrigation and drainage strategies to improve the quality of saline-alkali soil is vital for enhancing agricultural production and increasing economic returns. In this study, we explored how irrigation and drainage modes (flood irrigation, drip irrigation, and sub-surface pipe drainage under drip irrigation) improve the saline-alkali soil in Xinjiang, China. We aimed to study the transport characteristics of soil water and salt under different irrigation and drainage modes, and analyze the effects of the combination of irrigation and drainage on soil salt leaching, as well as its impacts on the growth of oil sunflower. Our results show that sub-surface pipe drainage under drip irrigation significantly reduced the soil salt content and soil water content at the 0–200 cm soil depth. Under sub-surface pipe drainage combined with drip irrigation, the mean soil salt content was reduced to below 10 g/kg after the second irrigation, and the soil salt content decreased as sub-surface pipe distance decreased. The mean soil salt content of flood irrigation exceeded 25 g/kg, and the mean soil desalination efficiency was 3.28%, which was lower than that of drip irrigation. The mean soil desalination rate under drip irrigation and sub-surface pipe drainage under drip irrigation was 19.30% and 58.12%, respectively. After sub-surface drainage regulation under drip irrigation, the germination percentage of oil sunflower seedlings was increased to more than 50%, which further confirmed that combined drip irrigation and sub-surface pipe drainage is very effective in improving the quality of saline-alkali soil and increasing the productivity of agricultural crops.

SUSTAINABLE AGRICULTURE DEVELOPMENT IN SALINE-ALKALI SOIL AREA OF SONGNEN PLAIN,NORTHEAST CHINA

There is great potential for agriculture in saline-alkali soil area in Songnen Plain, Northeast China. But the sustainable crop production in this area has been restricted by a few of main factors, such as less precipitation, h igher evaporation and frequent drought, high salinity and alkalinity, high excha ngeable sodium content and poor infiltration of the soil, and insufficiency and low availability in nutrition. It is also considered that there are a few of fav orable conditions for agricultural development in this region, such as sufficien t light and heat resources, rich ground water resources, plenty of manure produc ed by livestock, and so on. At the same time, scientific management and measurem ents have been employed; rational irrigation and drainage system has been establ ished; reclamation, amendment and fertilization of soil, and suitable strategies of cropping practices have been made for the sustainable development of agricul ture. Great progress has been made during 1996-2000.

Effects of forest cover types and environmental factors on soil respiration dynamics in a coastal sand dune of subtropical China

Trees on sand dunes are more sensitive to environmental changes because sandy soils have extremely low water holding capacity and nutrient availability. We investigated the dynamics of soil respiration(Rs) for secondary natural Litsea forest and plantations of casuarina,pine, acacia and eucalyptus. Results show that significant diurnal variations of Rsoccurred in autumn for the eucalyptus species and in summer for the pine species, with higher mean soil respiration at night. However, significant seasonal variations of Rswere found in all five forest stands. Rschanged exponentially with soil temperatures at the 10-cm depth; the models explain 43.3–77.0% of Rs variations. Positive relationships between seasonal Rsand soil moisture varied with stands. The correlations were significant only in the secondary forest, and the eucalyptus and pine plantations. The temperature sensitivity parameter(Q10 value) of Rsranged from 1.64 in casuarina plantation to 2.32 the in secondary forest; annual Rswas highest in the secondary forest and lowest in the pine plantation. The results indicate that soil temperatures and moisture are the primary environmental controls of soil respiration and mainly act through a direct influence on roots and microbial activity. Differences in root biomass, quality of litter,and soil properties(pH, total N, available P, and exchangeable Mg) were also significant factors.

The impact of land use change on quality evolution of soil genetic layers on the coastal plain of south Hangzhou Bay

Land use change plays an important part in the studies of global environmental change and regional sustainable development. The change of soil quality can particularly reflect the impacts of human socio-economic activities on environment. Taking the coastal plain of south Hangzhou Bay as a study case, we analyzed the effects of land use changes on organic matter （OM）, total nitrogen （TN）, total phosphorus （TP）, available phosphorus （AP）, available potassium （AK）, total salinity （TS）, pH value in soil genetic layers, and assessed soil quality change related to different land use types from 1982 to 2003. The results show that： （1） The general change tendency of soil quality in the coastal plain of south Hangzhou Bay declined obviously in A layer and slightly rise in B （or P） layer and C （or W） layer. The contents of TP decreased generally in all soil genetic layers, but the variety difference of other soil quality indices was relatively great. （2） The change of soil quality in the areas where land use changed is far more remarkable than that with land use unchanged. The value of quality variety is A layer 〉B （or P） layer 〉C （or W） layer. （3） The changes of soil tillage, cultivation, fertilization, irrigation and drainage activities related to land use may make some soil-forming processes disappeared and bring in other new processes which will affect the soil quality and soil genetic layers directly.

Effects of water application intensity of microsprinkler irrigation on water and salt environment and crop growth in coastal saline soils

Laboratory and field experiments were conducted to investigate the effects of water application intensity（WAI） on soil salinity management and the growth of Festuca arundinacea（festuca） under three stages of water and salt management strategies using microsprinkler irrigation in Hebei Province, North China. The soil water content（è） and salinity of homogeneous coastal saline soils were evaluated under different water application intensities in the laboratory experiment. The results indicated that the WAI of microsprinkler irrigation influenced the è, electrical conductivity（ECe） and p H of saline soils. As the WAI increased, the average values of è and ECe in the 0–40 cm profile also increased, while their average values in the 40–60 cm profile decreased. The p H value also slightly decreased as depth increased, but no significant differences were observed between the different treatments. The time periods of the water redistribution treatments had no obvious effects. Based on the results for è, ECe and p H, a smaller WAI was more desirable. The field experiment was conducted after being considered the results of the technical parameter experiment and evaporation, wind and leaching duration. The field experiment included three stages of water and salt regulation, based on three soil matric potentials（SMP）, in which the SMP at a 20-cm depth below the surface was used to trigger irrigation. The results showed that the microsprinkler irrigation created an appropriate environment for festuca growth through the three stages of water and salt regulation. The low-salinity conditions that occurred at 0–10 cm depth during the first stage（-5 k Pa） continued to expand through the next two stages. The average p H value was less than 8.5. The tiller number of festuca increased as SMP decreased from the first stage to the third stage. After the three stages of water and salt regulation, the highly saline soil gradually changed to a low-saline soil. Overall, based on the salt desalinization, the microsprinkler irrigation and three stages of water and salt regulation could be successfully used to cultivate plants for the reclamation of coastal saline land in North China.

Fertilization Affects Biomass Production of Suaeda salsa and Soil Organic Carbon Pool in East Coastal Region of China

Land use practice significantly affects soil properties. Soil is a major sink for atmospheric carbon, and soil organic carbon （SOC） is considered as an essential indicator of soil quality. The objective of this study was to assess the effects of N and P applied to Suaeda salsa on biomass production, SOC concentration, labile organic carbon （LOC） concentration, SOC pool and carbon management index （CMI） as well as the effect of the land use practice on soil quality of coastal tidal lands in east coastal region of China. The study provided relevant references for coastal exploitation, tidal land management and related study in other countries and regions. The field experiment was laid out in a randomized complete block design, consisting of four N-fertilization rates （0 （NO）, 60 （N1）, 120 （N2） and 180 kg ha-1 （N3））, three P-fertilization rates （0 （P0）, 70 （P1） and 105 kg ha-~ （P2）） and bare land without vegetation. N and P applied to S. salsa on coastal tidal lands significantly affected biomass production （above-ground biomass and roots）, bulk density （Pb）, available N and P, SOC, LOC, SOC pool and CMI. Using statistical analysis, significantly interactions in N and P were observed for biomass production and the dominant factor for S. salsa production was N in continuous 2-yr experiments. There were no significant interactions between N and P for SOC concentration, LOC concentration and SOC pool. However, significant interaction was obtained for CMI at the 0-20 cm depth and N played a dominant role in the variation of CMI. There were significant improvements for soil measured attributes and parameters, which suggested that increasing the rates of N and P significantly decreased Pb at the 0-20 cm depth and increased available N and P, SOC, LOC, SOC pool as well as CMI at both the 0-20 and 20-40 cm depth, respectively. By correlation analysis, there were significantly positive correlations between biomass （above- ground biomass and roots） and SOC as well as LOC in 2010 and 2011 across all soil depth, respectively. The treatment with N at 180 kg ha-~ and P at 105 kg ha-1 was superior to the other treatments. The results from the 2-yr continuous experiments indicated that, in short-term, there were a few accumulation of SOC and LOC concentrations by means of N and P application to S. salsa, whereas in the long run, S. salsa with N and P application was recommended for coastal tidal lands because of its great potential of carbon sequestration, improvements of soil nutrition status and promotion of soil quality.

Soil Carbon, Nitrogen and Phosphorus Concentrations and Stoichiometries Across a Chronosequence of Restored Inland Soda Saline-Alkali Wetlands, Western Songnen Plain, Northeast China

Soil carbon(C), nitrogen(N) and phosphorus(P) concentrations and stoichiometries can be used to evaluate the success indicators to the effects of wetland restoration and reflect ecosystem function. Restoration of inland soda saline-alkali wetlands is widespread, however, the soil nutrition changes that follow restoration are unclear. We quantified the recovery trajectories of soil physicochemical properties, including soil organic carbon(SOC), total nitrogen(TN), and total phosphorus(TP) pools, for a chronosequence of three restored wetlands(7 yr, 12 yr and 21 yr) and compared these properties to those of degraded and natural wetlands in the western Songnen Plain, Northeast China. Wetland degradation lead to the loss of soil nutrients. Relative to natural wetlands, the mean reductions of in SOC, TN, and TP concentrations were 89.6%, 65.5% and 52.5%, respectively. Nutrients recovered as years passed after restoration. The SOC, TN, and TP concentrations increased by 2.36 times, 1.15 times, and 0.83 times, respectively in degraded wetlands that had been restored for 21 yr, but remained 29.2%, 17.3%, and 12.8% lower, respectively, than those in natural wetlands. The soil C∶N(RC N), C∶P(R CP), and N∶P(R NP) ratios increased from 5.92 to 8.81, 45.36 to 79.19, and 7.67 to 8.71, respectively in the wetland that had been restored for 12 yr. These results were similar to those from the natural wetland and the wetland that had been restored for 21 yr(P > 0.05). Soil nutrients changes occurred mainly in the upper layers(≤ 30 cm), and no significant differences were found in deeper soils(> 30 cm). Based on this, we inferred that it would take at least 34 yr for SOC, TN, and TP concentrations and 12 yr for RC N, R CP, and RN P in the top soils of degraded wetlands to recover to levels of natural wetlands. Soil salinity negatively influenced SOC(r =-0.704, P < 0.01), TN(r =-0.722, P < 0.01), and TP(r =-0.882, P < 0.01) concentrations during wetland restoration, which indicates that reducing salinity is beneficial to SOC, TN, and TP recovery. Moreover, plants were an important source of soil nutrients and vegetation restoration was conducive to soil nutrient accumulation. In brief, wetland restoration increased the accumulation of soil biogenic elements, which indicated that positive ecosystem functions changes had occurred.

Coastal afforestation effects on soil properties at Hatiya in Bangladesh

An exploratory study was conducted in the coastal plantation （12- and 17-year-old Sonneratia apetala） of Char Alim and Char Piya and on their adjacent barren lands at Char Rehania and Char Nurul Islam in Hatiya of Noakhali district, in Bangladesh to determine afforestation effects on soil properties. At soil depths of 0-10, 10-30 and 30-40 cm across three different land strips viz. inland, middle and sea side in 12- and 17-year-old keora （Sonneratia apetala） plantations, soil moisture, particle density, organic matter and C, total N, pH, available P, K, Na, Ca and Mg were significantly （p≤0.05, p≤0.01, p≤0.001） higher, and soil salinity significantly （p〈0.001） lower than that in their adjacent barren lands. Soil moisture, particle density, organic matter and C, total N, pH, soil salinity, available P, K, Na, Ca and Mg of surface soil in Char Alim plantation at inland were 31.09%, 2.24 g.cm^-3, 2.41%, 4.14%, 0.58%, 7.07, 0.09 dS＇cm^-1, 28.06 mg.L^-1, 0.50 mg-L^- 1 11.5 mg-L^-1, 3.30 mg·L^-1 and 2.7 mmol.kg^-1, respectively. Their corresponding values for the same depth and land position at adjacent Char Rehania barren land were 16.69%, 1.25g.cm^-3, 0.43%, 0.74%, 0.25%, 6.57, 0.13 dS.cm^-1, 13.07mg-L^-1, 0.30 mg.L^-1, 1.4 mg.L^-1, 0.30 mmol·kg^-1 and 0.50 mg.L^-1, respectively. Soil moisture, particle density, organic matter and C, total N, pH, available P, K and Ca decreased, and soil salinity, available Na and Mg increased from inland towards sea side in the plantations. Although soil texture did not differ in most soil depths between plantation and adjacent barren land, proportion of sand particle was significantly （p≤0.01） lower and silt particle significantly （p〈0.001） in the plantations higher than that in their adjacent barren lands. In the study, evaluation of all the parameters was also done for the other pair of lands.

Combined influence of sedimentation and vegetation on the soil carbon stocks of a coastal wetland in the Changjiang estuary

Coastal wetlands play an important role in the global carbon cycle. Large quantities of sediment deposited in the Changjiang （Yangtze） estuary by the Changjiang River promote the propagation of coastal wetlands, the expansion of saltmarsh vegetation, and carbon sequestration. In this study, using the Chongming Dongtan Wetland in the Changjiang estuary as the study area, the spatial and temporal distribution of soil organic carbon （SOC） stocks and the influences of sedimentation and vegetation on the SOC stocks of the coastal wetland were examined in 2013. There was sediment accretion in the northern and middle areas of the wetland and in the Phragmites australis marsh in the southern area, and sediment erosion in the Scirpus mariqueter marsh and the bare mudflat in the southem area. More SOC accumulated in sediments of the vegetated marsh than in the bare mudflat. The total organic carbon （TOC） stocks increased in the above-ground biomass from spring to autumn and decreased in winter; in the below-ground biomass, they gradually increased from spring to winter. The TOC stocks were higher in the below-ground biomass than in the above-ground biomass in the P. australis and Spartina alterniflora marshes, but were lower in the below-ground biomass in S. mariqueter marsh. Stocks of SOC showed temporal variation and increased gradually in all transects from spring to winter. The SOC stocks tended to decrease from the high marsh down to the bare mudflat along the three transects in the order： P. australis marsh 〉 S. alterniflora marsh 〉 S. mariqueter marsh 〉 bare mudflat. The SOC stocks of the same vegetation type were higher in the northern and middle transects than in the southern transect. These results suggest that interactions between sedimentation and vegetation regulate the SOC stocks in the coastal wetland in the Changjiang estuary.

Sufficiency and Deficiency Indices of Soil Available Zinc for Rice in the Alluvial Soil of the Coastal Yellow Sea

To determine the sufficiency and deficiency indices of soil available Zn by the Agro Services International （ASI） method （ASI-Zn） for Zn fertilizer recommendation in rice production in the alluvial soil of the coastal Yellow Sea, the relationship between relative rice yield and soil available ASI-Zn concentration was analyzed from a ten-field experiment with various soil test classes ranging from low to high fertility in 2005 and 2006, and nine Zn fertilizer application rates （0, 7.5 15, 22.5, 30, 37.5, 45, 52.5 and 60 kg Zn/ha） arranged at random with three replications in each field. There was a significant quadratic relationship between soil available ASI-Zn and rice yield, and a significant linear relationship between soil available ASI-Zn concentration and Zn fertilization rate. For rice variety Wuyujing 3, soil available ASI-Zn was deficient when the value was at lower than 1 mg Zn/L, low at 1 to 2 mg Zn/L, sufficient at 1 to 2 mg Zn/L, excessive at higher than 7.5 mg Zn/L. Thus, Zn fertilizer recommendation could be done according to the sufficiency and deficiency indices of soil ASI-Zn. For most of alluvial soils of the coastal Yellow Sea in the study, the available ASI-Zn was lower than 1 mg Zn/L, and then the optimum application rate of Zn fertilizer was about 20 kg Zn/ha.

Effect of different water application intensity and irrigation amount treatments of microirrigation on soil-leaching coastal saline soils of North China

In coastal regions, Bohai Gulf is one of the most affected areas by salinization. To study the effects of mocrosprinkler irrigation on the characteristics of highly saline sandy loam soil（ECe（saturated paste extract）=22.3 d S m^–1; SAR（sodium adsorption ratio）=49.0） of North China, a laboratory experiment was conducted. Five water application intensity（WAI） treatments（1.7, 3.1, 5.3, 8.8, and 10.1 mm h^–1）, five irrigation amount（IA） treatments（148, 168, 184, 201, and 223 mm） and three time periods of water redistribution（0, 24 and 48 h） were employed in the study. A compounding microsprinkler system was used for the WAI treatments, and a single microsprinkler was used for the IA treatments. The results indicated that, as soil depth increased, soil water content（θ） increased and then slightly decreased; with WAI and IA consistently increasing, the relatively moist region expanded and the average θ increased. Meanwhile, soil ECe increased as soil depth increased, and the zone with low soil salinity expanded as WAI and IA increased. Although the reduction of the average SAR was smaller than that of the average electrical conductivity of the ECe, these variables decreased in similar fashion as WAI and IA increased under microsprinkler irrigation. The average p H decreased as soil depth increased. Longer time periods of water redistribution led to lower salinity and slight expansion of the SAR zone. Considering the effects of leached salts in coastal saline soils, greater WAI and IA values are more advantageous under unsaturated flow conditions, as they cause better water movement in the soil. After leaching due to microsprinkler irrigation, highly saline soil gradually changes to moderately saline soil. The results provide theoretical and technological guidance for the salt leaching and landscaping of highly saline coastal environments.

Significant changes in arbuscular mycorrhizal community and soil physicochemical properties during the saline-alkali grassland vegetation succession

Arbuscular mycorrhizal(AM)fungi are widely distributed in various habitats,and the community composition varies in response to the changing environmental conditions.To explore the response of community composition to the succession of saline-alkali land,soil samples were collected from three succession stages of Songnen saline-alkali grassland.Subsequently,the soil characteristics were determined and the AM fungi in soil samples were analyzed by high-throughput sequencing.Then,the response relationship between community composition and soil characteristics was studied by Canonical correlation and Pearson analyses.The soil properties improved with the succession of saline-alkali grassland.There was no significant difference in alpha diversity between the first and second succession stage(Suaeda glauca and Puccinellia tenuiflora,respectively),and the microbial community had a dense association network at the third stage(Leymus chinensis);in addition,each succession stage had significantly enriched amplicon sequence variants(ASVs)and functional pathways.All the soil properties except cellulase activity had significant effects on community composition.Furthermore,the pH,organic carbon,organic matter,and sucrase activity significantly correlated with alpha diversity indices.These results provide a theoretical basis for realizing the significant changes in AM fungal community and soil properties during the saline-alkali grassland vegetation succession.

Effects of Reclamation on Soil Carbon and Nitrogen in Coastal Wetlands of Liaohe River Delta,China

To evaluate the influence of wetland reclamation on vertical distribution of carbon and nitrogen in coastal wetland soils, we measured the soil organic carbon(SOC), soil total nitrogen(STN) and selected soil properties at five sampling plots(reed marsh, paddy field, corn field, forest land and oil-polluted wetland) in the Liaohe River estuary in September 2013. The results showed that reclamation significantly changed the contents of SOC and STN in the Liaohe River estuary(P < 0.001). The SOC concentrations were in the order: oil-polluted wetland > corn field > paddy field > forest land > reed marsh, with mean values of 52.17, 13.14, 11.46, 6.44 and 6.16 g/kg, respectively. STN followed a similar order as SOC, with mean values of 1351.14, 741.04, 632.32, 496.17 and 390.90 mg/kg, respectively. Interaction of reclamation types and soil depth had significant effects on SOC and STN, while soil depth had significant effects on SOC, but not on STN. The contents of SOC and STN were negatively correlated with pH and redox potential(Eh) in reed marsh and corn field, while the SOC and STN in paddy field had positive correlations with electrical conductivity(EC). Dissolved organic carbon(DOC), ammonium nitrogen(NH_4^+-N) and nitrate nitrogen(NO_3~–-N) were also significantly changed by human activities. NH_4^+-N and NO_3~–-N increased to different degrees, and forest land had the highest NO_3~–-N concentration and lowest DOC concentration, which could have been caused by differences in soil aeration and fertilization. Overall, the results indicate that reed harvest increased soil carbon and nitrogen release in the Liaohe River Estuary, while oil pollution significantly increased the SOC and STN; however, these cannot be used as indicators of soil fertility and quality because of the serious oil pollution.