A new cobalt(Ⅱ)-radical complex:[Co(im4-py)_(2)(PNB)_(2)](im4-py=2-(4'-pyridyl)-4,4,5,5-tetramethylimidazole-1-oxyl,HPNB=p-nitrobenzoic acid)has been synthesized and characterized by X-ray diffraction analysis,el...A new cobalt(Ⅱ)-radical complex:[Co(im4-py)_(2)(PNB)_(2)](im4-py=2-(4'-pyridyl)-4,4,5,5-tetramethylimidazole-1-oxyl,HPNB=p-nitrobenzoic acid)has been synthesized and characterized by X-ray diffraction analysis,elemental analysis,IR,and magnetic properties.X-ray diffraction analysis shows that the complex exists as mononuclear molecules and Co(Ⅱ)ion is four-coordinated with two radicals and two PNB-ligands.The magnetic susceptibility study indicates the complex exhibits weak ferromagnetic interactions between cobalt(Ⅱ)and im4-py radical.The magnetic property is explained by the magnetic and structure exchange mechanism.CCDC:976028.展开更多
目的探究三萜皂苷黄芪皂苷Ⅱ(AstragalosideⅡ,ASⅡ)对三阴乳腺癌(Triple nega⁃tive breast cancer,TNBC)细胞增殖及侵袭转移的作用及可能的机制。方法采用MTT法检测黄芪皂苷Ⅱ(0.78μM、1.56μM、3.12μM、6.25μM、12.5μM、25μM、50...目的探究三萜皂苷黄芪皂苷Ⅱ(AstragalosideⅡ,ASⅡ)对三阴乳腺癌(Triple nega⁃tive breast cancer,TNBC)细胞增殖及侵袭转移的作用及可能的机制。方法采用MTT法检测黄芪皂苷Ⅱ(0.78μM、1.56μM、3.12μM、6.25μM、12.5μM、25μM、50μM、100μM)对MDA-MB-231细胞增殖的影响;采用Transwell法及划痕实验分别检测黄芪皂苷Ⅱ对MDA-MB-231细胞转移及侵袭的影响;采用Western blot检测PI3K/AKT/mTOR通路蛋白的表达水平,从而阐明其调控PI3K/AKT/mTOR通路的分子机制。结果25μM黄芪皂苷Ⅱ显著抑制MDA-MB-231细胞增殖,选用未影响细胞增殖的药物浓度3.12μM、6.25μM、12.5μM作为低、中、高药物浓度;与对照组相比,黄芪皂苷Ⅱ呈浓度依赖性地抑制MDA-MB-231细胞的侵袭与转移;同时,该成分抑制MDA-MB-231细胞PI3K、AKT、mTOR蛋白磷酸化水平。结论黄芪皂苷Ⅱ可能通过调控PI3K/AKT/mTOR通路抑制MDA-MB-231细胞增殖、侵袭及转移。展开更多
This work reveals the significant effects of cobalt(Co)on the microstructure and impact toughness of as-quenched highstrength steels by experimental characterizations and thermo-kinetic analyses.The results show that ...This work reveals the significant effects of cobalt(Co)on the microstructure and impact toughness of as-quenched highstrength steels by experimental characterizations and thermo-kinetic analyses.The results show that the Co-bearing steel exhibits finer blocks and a lower ductile-brittle transition temperature than the steel without Co.Moreover,the Co-bearing steel reveals higher transformation rates at the intermediate stage with bainite volume fraction ranging from around 0.1 to 0.6.The improved impact toughness of the Co-bearing steel results from the higher dense block boundaries dominated by the V1/V2 variant pair.Furthermore,the addition of Co induces a larger transformation driving force and a lower bainite start temperature(BS),thereby contributing to the refinement of blocks and the increase of the V1/V2 variant pair.These findings would be instructive for the composition,microstructure design,and property optimization of high-strength steels.展开更多
文摘A new cobalt(Ⅱ)-radical complex:[Co(im4-py)_(2)(PNB)_(2)](im4-py=2-(4'-pyridyl)-4,4,5,5-tetramethylimidazole-1-oxyl,HPNB=p-nitrobenzoic acid)has been synthesized and characterized by X-ray diffraction analysis,elemental analysis,IR,and magnetic properties.X-ray diffraction analysis shows that the complex exists as mononuclear molecules and Co(Ⅱ)ion is four-coordinated with two radicals and two PNB-ligands.The magnetic susceptibility study indicates the complex exhibits weak ferromagnetic interactions between cobalt(Ⅱ)and im4-py radical.The magnetic property is explained by the magnetic and structure exchange mechanism.CCDC:976028.
文摘目的探究三萜皂苷黄芪皂苷Ⅱ(AstragalosideⅡ,ASⅡ)对三阴乳腺癌(Triple nega⁃tive breast cancer,TNBC)细胞增殖及侵袭转移的作用及可能的机制。方法采用MTT法检测黄芪皂苷Ⅱ(0.78μM、1.56μM、3.12μM、6.25μM、12.5μM、25μM、50μM、100μM)对MDA-MB-231细胞增殖的影响;采用Transwell法及划痕实验分别检测黄芪皂苷Ⅱ对MDA-MB-231细胞转移及侵袭的影响;采用Western blot检测PI3K/AKT/mTOR通路蛋白的表达水平,从而阐明其调控PI3K/AKT/mTOR通路的分子机制。结果25μM黄芪皂苷Ⅱ显著抑制MDA-MB-231细胞增殖,选用未影响细胞增殖的药物浓度3.12μM、6.25μM、12.5μM作为低、中、高药物浓度;与对照组相比,黄芪皂苷Ⅱ呈浓度依赖性地抑制MDA-MB-231细胞的侵袭与转移;同时,该成分抑制MDA-MB-231细胞PI3K、AKT、mTOR蛋白磷酸化水平。结论黄芪皂苷Ⅱ可能通过调控PI3K/AKT/mTOR通路抑制MDA-MB-231细胞增殖、侵袭及转移。
基金supported by the National Natural Science Foundation of China(No.52271089)the financial support from the C hina Postdoctoral Science Foundation(No.2023M732192)。
文摘This work reveals the significant effects of cobalt(Co)on the microstructure and impact toughness of as-quenched highstrength steels by experimental characterizations and thermo-kinetic analyses.The results show that the Co-bearing steel exhibits finer blocks and a lower ductile-brittle transition temperature than the steel without Co.Moreover,the Co-bearing steel reveals higher transformation rates at the intermediate stage with bainite volume fraction ranging from around 0.1 to 0.6.The improved impact toughness of the Co-bearing steel results from the higher dense block boundaries dominated by the V1/V2 variant pair.Furthermore,the addition of Co induces a larger transformation driving force and a lower bainite start temperature(BS),thereby contributing to the refinement of blocks and the increase of the V1/V2 variant pair.These findings would be instructive for the composition,microstructure design,and property optimization of high-strength steels.
