Helicobacter pylori (H. pylori) can infect into the epithelial cell to cause benign or malignant disorders. Under stressful environment, a spiral form of H. pylori is transformed into a coccoid form. The infectivity o...Helicobacter pylori (H. pylori) can infect into the epithelial cell to cause benign or malignant disorders. Under stressful environment, a spiral form of H. pylori is transformed into a coccoid form. The infectivity of the coccoid form is still controversial. Since spiral forms are transformed into two types of coccoid forms via different mechanisms, the infectivity of the two types of coccoid forms into human gastric epithelial cell was examined. A laboratory and a clinical strain of H. pyloriv were cultured in liquid medium under different conditions to produce the two types of coccoid forms. These coccoid H. pylorisv were then co-cultured with human derived gastric epithelial cell, MKN-28. Adhesion and penetration of bacteria into MKN-28 cells were monitored by scanning-, standard transmission- and immunotransmission-electron microscopy (SEM, TEM and ITEM). We observed that both coccoid forms were able to adhere onto the surface of MKN-28 cells in agminated formation and also penetrated into the gastric epithelial cells besides the spiral form of H. pyloriv. Coccoid H. pylori is not a passive entity but can actively infect the human gastric epithelial cell.展开更多
The presence of viable Helicobacter pylori(H. pylori) in the environment is considered to contribute to the levels of H. pylori found in the human population, which also aids to increase its genetic variability and it...The presence of viable Helicobacter pylori(H. pylori) in the environment is considered to contribute to the levels of H. pylori found in the human population, which also aids to increase its genetic variability and its environment adaptability and persistence. H. pylori form biofilms both within the in vitro and in vivo envi-ronment. This represents an important attribute that assists the survival of this bacterium within environ-ments that are both hostile and adverse to prolifera-tion. It is the aim of this paper to review the ability of H. pylori to form biofilms in vivo and in vitro and to address the inherent mechanisms considered to sig-nificantly enhance its persistence within the host and in external environments. Furthermore, the dissemi-nation of H. pylori in the external environment and within in the human body and its impact upon infec-tion control shall be discussed.展开更多
BACKGROUND Helicobacter pylori(H.pylori)is a spiral-shaped bacterium responsible for the development of chronic gastritis,gastric ulcer,gastric cancer(GC),and MALTlymphoma of the stomach.H.pylori can be present in the...BACKGROUND Helicobacter pylori(H.pylori)is a spiral-shaped bacterium responsible for the development of chronic gastritis,gastric ulcer,gastric cancer(GC),and MALTlymphoma of the stomach.H.pylori can be present in the gastric mucosa(GM)in both spiral and coccoid forms.However,it is not known whether the severity of GM contamination by various vegetative forms of H.pylori is associated with clinical and morphological characteristics and long-term results of GC treatment.AIM To establish the features of H.pylori infection in patients with GC and their correlations with clinical and morphological characteristics of diseases and long-term results of treatment.METHODS Of 109 patients with GC were included in a prospective cohort study.H.pylori in the GM and tumor was determined by rapid urease test and by immunohistochemically using the antibody to H.pylori.The results obtained were compared with the clinical and morphological characteristics and prognosis of GC.Statistical analysis was performed using the Statistica 10.0 software.RESULTS H.pylori was detected in the adjacent to the tumor GM in 84.5%of cases,of which a high degree of contamination was noted in 50.4%of the samples.Coccoid forms of H.pylori were detected in 93.4%of infected patients,and only coccoid-in 68.9%.It was found that a high degree of GM contamination by the coccoid forms of H.pylori was observed significantly more often in diffuse type of GC(P=0.024),in poorly differentiated GC(P=0.011),in stage T3-4(P=0.04)and in N1(P=0.011).In cases of moderate and marked concentrations of H.pylori in GM,a decrease in 10-year relapse free and overall survival from 55.6%to 26.3%was observed(P=0.02 and P=0.07,respectively).The relationship between the severity of the GM contamination by the spiral-shaped forms of H.pylori and the clinical and morphological characteristics and prognosis of GC was not revealed.CONCLUSION The data obtained indicates that H.pylori may be associated not only with induction but also with the progression of GC.展开更多
AIM To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS Blood samples were collected from 278 patients with gastric diseases. A 3 day old culture of H. pylori...AIM To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS Blood samples were collected from 278 patients with gastric diseases. A 3 day old culture of H. pylori on chocolate blood agar was used to provide spiral form. ‘Synchronous’ coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0 4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori . RESULTS Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non ulcer dyspepsia (NUD) (44%) and other diseases (45%) ( P <0 05). IgG antibodies against spiral and coccoid antigens were detected in 50 7% (141/278) and 49 6% (138/278) , respectively. CONCLUSION The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.展开更多
目的探讨螺杆状和球形 H ．pylori 在胃和十二指肠球部黏膜中的分布特点及其意义。方法纳入2014年11月1日至2015年3月30日行胃镜检查的101例有上腹部不适、疼痛等消化道症状的患者，钳取胃角、胃体、胃窦和十二指肠球部黏膜，采用 HE ...目的探讨螺杆状和球形 H ．pylori 在胃和十二指肠球部黏膜中的分布特点及其意义。方法纳入2014年11月1日至2015年3月30日行胃镜检查的101例有上腹部不适、疼痛等消化道症状的患者，钳取胃角、胃体、胃窦和十二指肠球部黏膜，采用 HE 染色、银染色和免疫组织化学染色检测两种形态 H ．pylori。统计学方法采用卡方检验。结果螺杆状 H ．pylori 阳性率为47．5％（48／101），球形 H ．pylori 阳性率为62．4％（63／101）。螺杆状 H ．pylori 阳性者中，十二指肠球部黏膜螺杆状H ．pylori阳性率为29．2％（14／48），低于胃角[83．3％（40／48）]、胃体[83．3％（40／48）]和胃窦[77．0％（37／48）]，差异均有统计学意义（χ2＝14．447、10．318、12．314，P 均＜0．01）。单纯球形 H ．pylori 感染的19例患者中，胃窦黏膜球形 H ．pylori 阳性率（16／19）高于胃角（7／19）和胃体（8／19），差异均有统计学意义（χ2＝15．784、7．238，P 均＜0．01）。胃窦黏膜螺杆状与球形 H ．pylori 重度感染率分别为45．9％（17／37）和16．7％（9／54），分别高于胃角[17．5％（7／40）和0（0／40）]、胃体[22．5％（9／40）和0（0／48）]和十二指肠球部黏膜[0／14，2．4％（1／41）]，差异均有统计学意义（χ2＝7．230、4．711、9．636，7．373、10．066、5．083，P 均＜0．05）。胃体、胃窦和十二指肠球部黏膜细胞内或间质中的中重度球形 H ．pylori感染率分别为56．0％（14／25）、68．6％（24／35）和53．1％（17／32），分别高于黏液中[17．4％（4／23）、4／19、0／9]，差异均有统计学意义（χ2＝7．619、11．138、6．126，P 均＜0．01）。结论螺杆状 H ．pylori 主要分布于胃，单纯球形 H ．pylori 感染以胃窦为主，两种形态 H ．pylori 感染强度均以胃窦黏膜严重。胃体、胃窦和十二指肠球部球形H ．pylori定植深度与感染强度有关。展开更多
文摘Helicobacter pylori (H. pylori) can infect into the epithelial cell to cause benign or malignant disorders. Under stressful environment, a spiral form of H. pylori is transformed into a coccoid form. The infectivity of the coccoid form is still controversial. Since spiral forms are transformed into two types of coccoid forms via different mechanisms, the infectivity of the two types of coccoid forms into human gastric epithelial cell was examined. A laboratory and a clinical strain of H. pyloriv were cultured in liquid medium under different conditions to produce the two types of coccoid forms. These coccoid H. pylorisv were then co-cultured with human derived gastric epithelial cell, MKN-28. Adhesion and penetration of bacteria into MKN-28 cells were monitored by scanning-, standard transmission- and immunotransmission-electron microscopy (SEM, TEM and ITEM). We observed that both coccoid forms were able to adhere onto the surface of MKN-28 cells in agminated formation and also penetrated into the gastric epithelial cells besides the spiral form of H. pyloriv. Coccoid H. pylori is not a passive entity but can actively infect the human gastric epithelial cell.
文摘The presence of viable Helicobacter pylori(H. pylori) in the environment is considered to contribute to the levels of H. pylori found in the human population, which also aids to increase its genetic variability and its environment adaptability and persistence. H. pylori form biofilms both within the in vitro and in vivo envi-ronment. This represents an important attribute that assists the survival of this bacterium within environ-ments that are both hostile and adverse to prolifera-tion. It is the aim of this paper to review the ability of H. pylori to form biofilms in vivo and in vitro and to address the inherent mechanisms considered to sig-nificantly enhance its persistence within the host and in external environments. Furthermore, the dissemi-nation of H. pylori in the external environment and within in the human body and its impact upon infec-tion control shall be discussed.
文摘BACKGROUND Helicobacter pylori(H.pylori)is a spiral-shaped bacterium responsible for the development of chronic gastritis,gastric ulcer,gastric cancer(GC),and MALTlymphoma of the stomach.H.pylori can be present in the gastric mucosa(GM)in both spiral and coccoid forms.However,it is not known whether the severity of GM contamination by various vegetative forms of H.pylori is associated with clinical and morphological characteristics and long-term results of GC treatment.AIM To establish the features of H.pylori infection in patients with GC and their correlations with clinical and morphological characteristics of diseases and long-term results of treatment.METHODS Of 109 patients with GC were included in a prospective cohort study.H.pylori in the GM and tumor was determined by rapid urease test and by immunohistochemically using the antibody to H.pylori.The results obtained were compared with the clinical and morphological characteristics and prognosis of GC.Statistical analysis was performed using the Statistica 10.0 software.RESULTS H.pylori was detected in the adjacent to the tumor GM in 84.5%of cases,of which a high degree of contamination was noted in 50.4%of the samples.Coccoid forms of H.pylori were detected in 93.4%of infected patients,and only coccoid-in 68.9%.It was found that a high degree of GM contamination by the coccoid forms of H.pylori was observed significantly more often in diffuse type of GC(P=0.024),in poorly differentiated GC(P=0.011),in stage T3-4(P=0.04)and in N1(P=0.011).In cases of moderate and marked concentrations of H.pylori in GM,a decrease in 10-year relapse free and overall survival from 55.6%to 26.3%was observed(P=0.02 and P=0.07,respectively).The relationship between the severity of the GM contamination by the spiral-shaped forms of H.pylori and the clinical and morphological characteristics and prognosis of GC was not revealed.CONCLUSION The data obtained indicates that H.pylori may be associated not only with induction but also with the progression of GC.
文摘AIM To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS Blood samples were collected from 278 patients with gastric diseases. A 3 day old culture of H. pylori on chocolate blood agar was used to provide spiral form. ‘Synchronous’ coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0 4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori . RESULTS Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non ulcer dyspepsia (NUD) (44%) and other diseases (45%) ( P <0 05). IgG antibodies against spiral and coccoid antigens were detected in 50 7% (141/278) and 49 6% (138/278) , respectively. CONCLUSION The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.
文摘目的探讨螺杆状和球形 H ．pylori 在胃和十二指肠球部黏膜中的分布特点及其意义。方法纳入2014年11月1日至2015年3月30日行胃镜检查的101例有上腹部不适、疼痛等消化道症状的患者，钳取胃角、胃体、胃窦和十二指肠球部黏膜，采用 HE 染色、银染色和免疫组织化学染色检测两种形态 H ．pylori。统计学方法采用卡方检验。结果螺杆状 H ．pylori 阳性率为47．5％（48／101），球形 H ．pylori 阳性率为62．4％（63／101）。螺杆状 H ．pylori 阳性者中，十二指肠球部黏膜螺杆状H ．pylori阳性率为29．2％（14／48），低于胃角[83．3％（40／48）]、胃体[83．3％（40／48）]和胃窦[77．0％（37／48）]，差异均有统计学意义（χ2＝14．447、10．318、12．314，P 均＜0．01）。单纯球形 H ．pylori 感染的19例患者中，胃窦黏膜球形 H ．pylori 阳性率（16／19）高于胃角（7／19）和胃体（8／19），差异均有统计学意义（χ2＝15．784、7．238，P 均＜0．01）。胃窦黏膜螺杆状与球形 H ．pylori 重度感染率分别为45．9％（17／37）和16．7％（9／54），分别高于胃角[17．5％（7／40）和0（0／40）]、胃体[22．5％（9／40）和0（0／48）]和十二指肠球部黏膜[0／14，2．4％（1／41）]，差异均有统计学意义（χ2＝7．230、4．711、9．636，7．373、10．066、5．083，P 均＜0．05）。胃体、胃窦和十二指肠球部黏膜细胞内或间质中的中重度球形 H ．pylori感染率分别为56．0％（14／25）、68．6％（24／35）和53．1％（17／32），分别高于黏液中[17．4％（4／23）、4／19、0／9]，差异均有统计学意义（χ2＝7．619、11．138、6．126，P 均＜0．01）。结论螺杆状 H ．pylori 主要分布于胃，单纯球形 H ．pylori 感染以胃窦为主，两种形态 H ．pylori 感染强度均以胃窦黏膜严重。胃体、胃窦和十二指肠球部球形H ．pylori定植深度与感染强度有关。
