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汉英温觉词“冷/cold”的隐喻对比研究
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作者 吕兆格 唐远远 《开封文化艺术职业学院学报》 2024年第5期62-66,共5页
温度是人类最早的认知领域之一。汉语和英语中存在大量描述温度感觉的词语,它们既指身体感知到的温度概念,又能映射到人类的心理态度等范畴,表达由温度义引申出的其他概念。文章从认知语言学的视角出发,借助隐喻理论,系统梳理汉英温觉... 温度是人类最早的认知领域之一。汉语和英语中存在大量描述温度感觉的词语,它们既指身体感知到的温度概念,又能映射到人类的心理态度等范畴,表达由温度义引申出的其他概念。文章从认知语言学的视角出发,借助隐喻理论,系统梳理汉英温觉词“冷/cold”基于身体认知的隐喻映射,得出语域映射的基本模式和结论。此外,受语言文化等因素的影响,“冷/cold”的隐喻映射也呈现出一种同异并存的现象。 展开更多
关键词 cold 温觉词 隐喻 汉英对比
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Atmospheric-Pressure DBD Cold Plasma for Preparation of High Active Au/P25 Catalysts for Low-Temperature CO Oxidation 被引量:3
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作者 底兰波 詹志彬 +2 位作者 张秀玲 亓滨 徐伟杰 《Plasma Science and Technology》 SCIE EI CAS CSCD 2016年第5期544-548,共5页
Cold plasma generated by dielectric barrier discharge (DBD) at atmospheric pressure was adopted for preparation of commercial TiO2 Degussa P25 supported Au catalysts (Au/P25- P) with the assistance of the depositi... Cold plasma generated by dielectric barrier discharge (DBD) at atmospheric pressure was adopted for preparation of commercial TiO2 Degussa P25 supported Au catalysts (Au/P25- P) with the assistance of the deposition-precipitation procedure. The influences of the plasma reduction time and calcination on the performance of the Au/P25-P catalysts were investigated. CO oxidation was performed to investigate the catalytic activity of the Au/P25 catalysts. The results show that DBD cold plasma for the fabrication of Au/P25-P catalysts is a fast process, and Au/P25-P (4 min) exhibited the highest CO oxidation activity due to the complete reduction of Au compounds and less consumption of oxygen vacancies. In order to form more oxygen vacancies active species, Au/P25-P was calcined to obtain Au/P25-PC catalysts. Interestingly, Au/P25- PC exhibited the highest activity for CO oxidation among the Au/P25 samples. The results of transmission electron microscopy (TEM) indicated that the smaller size and high distribution of Au nanoparticles are the mean reasons for a high performance of Au/P25-PC. Atmospheric- pressure DBD cold plasma was proved to be of great efficiency in preparing high performance supported Au catalysts. 展开更多
关键词 atmospheric-pressure cold plasma dielectric barrier discharge (DBD) Au TiO2 CO oxidation
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Partially crystallized Pd nanoparticles decorated TiO_2 prepared by atmospheric-pressure cold plasma and its enhanced photocatalytic performance 被引量:2
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作者 Zhijian Xu Bin Qi +1 位作者 Lanbo Di Xiuling Zhang 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2014年第6期679-683,共5页
TiO2 decorated with partially crystallized Pd nanoparticles (Pd/TiO2-P) was successfully prepared by atmospheric-pressure dielectric barrier discharge cold plasma. The XRD and XPS analyses proved that Pd ions were r... TiO2 decorated with partially crystallized Pd nanoparticles (Pd/TiO2-P) was successfully prepared by atmospheric-pressure dielectric barrier discharge cold plasma. The XRD and XPS analyses proved that Pd ions were reduced to partially crystallized metallic Pd nanoparticles in Pd/TiO2-P. The XPS spectra also indicated that an enhanced metal-support interaction was formed due to the existence of partially crystallized Pd nanoparticles with lower coordination number in Pd/TiO2-P. Photocatalytic activity of Pd/TiO2-P was much higher than that of TiO2 samples decorated with well crystallized Pd nanoparticles. 展开更多
关键词 Pd/TiO2 Pd with poor crystallinity PHOTOCATALYSIS atmospheric-pressure cold plasma dielectric barrier discharge
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Tuning Effect of N_2 on Atmospheric-Pressure Cold Plasma CVD of TiO_2 Photocatalytic Films 被引量:2
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作者 底兰波 李小松 +3 位作者 赵天亮 常大磊 刘倩倩 朱爱民 《Plasma Science and Technology》 SCIE EI CAS CSCD 2013年第1期64-69,共6页
To deposit TiO2 films through plasma CVD, the partial pressure ratio of O2 to TIC14 should be greater than the stoichiometric ratio (PO2/PTiCl4 〉 1). However, this may lead to the formation of powder instead of fil... To deposit TiO2 films through plasma CVD, the partial pressure ratio of O2 to TIC14 should be greater than the stoichiometric ratio (PO2/PTiCl4 〉 1). However, this may lead to the formation of powder instead of film on the substrate when using volume dielectric barrier discharge (volume-DBD) at atmospheric pressure. In this study, by adding N2 into the working gas Ar, TiO2 photocatalytic films were successfully fabricated in the presence of excess O2 (PO2/PTiC14 = 2.6) by using a wire-to-plate atmospheric-pressure volume-DBD. The tuning effect of N2 on the deposition of TiO2 film was studied in detail. The results showed that by increasing the N2 content, the deposition rate and particle size of the TiO2 film were reduced, and its photocatalytic activity was enhanced. The tuning mechanism of N2 is further discussed. 展开更多
关键词 plasma CVD TiO2 photocatalytic films atmospheric-pressure cold plasma dielectric barrier discharge (DBD) optical emission spectra (OES)
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Frontogenesis and Frontolysis of a Cold Filament Driven by the Cross-Filament Wind and Wave Fields Simulated by a Large Eddy Simulation 被引量:1
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作者 Guojing LI Dongxiao WANG +3 位作者 Changming DONG Jiayi PAN Yeqiang SHU Zhenqiu ZHANG 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第3期509-528,共20页
The variations of the frontogenetic trend of a cold filament induced by the cross-filament wind and wave fields are studied by a non-hydrostatic large eddy simulation. Five cases with different strengths of wind and w... The variations of the frontogenetic trend of a cold filament induced by the cross-filament wind and wave fields are studied by a non-hydrostatic large eddy simulation. Five cases with different strengths of wind and wave fields are studied.The results show that the intense wind and wave fields further break the symmetries of submesoscale flow fields and suppress the levels of filament frontogenesis. The changes of secondary circulation directions—that is, the conversion between the convergence and divergence of the surface cross-filament currents with the downwelling and upwelling jets in the filament center—are associated with the inertial oscillation. The filament frontogenesis and frontolysis caused by the changes of secondary circulation directions may periodically sharpen and smooth the gradient of submesoscale flow fields.The lifecycle of the cold filament may include multiple stages of filament frontogenesis and frontolysis. 展开更多
关键词 cold filament FRONTOGENESIS FRONTOLYSIS large eddy simulation
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Synthesis of Cu-Doped Mixed-Phase TiO_2 with the Assistance of Ionic Liquid by Atmospheric-Pressure Cold Plasma
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作者 詹志彬 底兰波 +1 位作者 张秀玲 李燕春 《Plasma Science and Technology》 SCIE EI CAS CSCD 2016年第5期494-499,共6页
An atmospheric-pressure dielectric barrier discharge (DBD) gas-liquid cold plasma was employed to synthesize Cu-doped TiO~ nanoparticles in an aqueous solution with the assistance of [C2MIM]BF4 ionic liquid (IL) a... An atmospheric-pressure dielectric barrier discharge (DBD) gas-liquid cold plasma was employed to synthesize Cu-doped TiO~ nanoparticles in an aqueous solution with the assistance of [C2MIM]BF4 ionic liquid (IL) and using air as the working gas. The influences of the discharge voltage, IL and the amount of copper nitrite were investigated. X-ray diffraction, N2 adsorption-desorption measurements and UV-Vis spectroscopy were adopted to characterize the samples. The results showed that the specific surface area of TiO2 was promoted with Cu-doping (from 57.6 m^2.g^-1 to 106.2 m^2.g^-1 with 3% Cu-doping), and the content of anatase was increased. Besides, the band gap energy of TiO~ with Cu-doping decreased according to the UV-Vis spec- troscopy test. The 3%Cu-IL-TiO2 samples showed the highest eificiency in degrading methylene blue (MB) dye solutions under simulated sunlight with an apparent rate constant of 0.0223 min-1, which was 1.2 times higher than that of non-doped samples. According to the characterization results, the reasons for the high photocatalytic activity were discussed. 展开更多
关键词 dielectric barrier discharge (DBD) gas-liquid cold plasma atmospheric pres-sure mixed-phase Ti02 Cu-doped
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Extreme Antarctic Cold of Late Winter 2023 被引量:1
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作者 Anastasia J.TOMANEK David E.MIKOLAJCZYK +7 位作者 Matthew A.LAZZARA Stefano DI BATTISTA Minghu DING Mariana FONTOLAN LITELL David H.BROMWICH Taylor P.NORTON Linda M.KELLER Lee J.WELHOUSE 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第10期1873-1880,共8页
Extreme cold temperatures were observed in July and August 2023,coinciding with the WINFLY(winter fly-in)period of mid to late August into September 2023,meaning aircraft operations into McMurdo Station and Phoenix Ai... Extreme cold temperatures were observed in July and August 2023,coinciding with the WINFLY(winter fly-in)period of mid to late August into September 2023,meaning aircraft operations into McMurdo Station and Phoenix Airfield were adversely impacted.Specifically,with temperatures below−50℃,safe flight operation was not possible because of the risk of failing hydraulics and fuel turning to gel onboard the aircraft.The cold temperatures were measured across a broad area of the Antarctic,from East Antarctica toward the Ross Ice Shelf,and stretching across West Antarctica to the Antarctic Peninsula.A review of automatic weather station measurements and staffed station observations revealed a series of sites recording new record low temperatures.Four separate cold phases were identified,each a few days in duration and occurring from mid-July to the end of August 2023.A brief analysis of 500-hPa geopotential height anomalies shows how the mid-tropospheric atmospheric environment evolves in relation to these extreme cold temperatures.The monthly 500-hPa geopotential height anomalies show strong negative anomalies in August.Examination of composite geopotential height anomalies during each of the four cold phases suggests various factors leading to cold temperatures,including both southerly off-content flow and calm atmospheric conditions.Understanding the atmospheric environment that leads to such extreme cold temperatures can improve prediction of such events and benefit Antarctic operations and the study of Antarctic meteorology and climatology. 展开更多
关键词 ANTARCTICA extreme cold temperature automatic weather station networks
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MIR1868 negatively regulates rice cold tolerance at both the seedling and booting stages 被引量:1
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作者 Yang Shen Xiaoxi Cai +7 位作者 Yan Wang Wanhong Li Dongpeng Li Hao Wu Weifeng Dong Bowei Jia Mingzhe Sun Xiaoli Sun 《The Crop Journal》 SCIE CSCD 2024年第2期375-383,共9页
Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive s... Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive stages.This study revealed a rice-specific 24-nt miRNA,miR1868,whose accumulation was suppressed by cold stress.Knockdown of MIR1868 increased seedling survival,pollen fertility,seed setting,and grain yield under cold stress,whereas its overexpression conferred the opposite phenotype.Knockdown of MIR1868 increased reactive oxygen species(ROS)scavenging and soluble sugar content under cold stress by increasing the expression of peroxidase genes and sugar metabolism genes,and its overexpression produced the opposite effect.Thus,MIR1868 negatively regulated rice cold tolerance via ROS scavenging and sugar accumulation. 展开更多
关键词 RICE cold tolerance MIRNA ROS scavenging Soluble sugar accumulation
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PHD17 acts as a target of miR1320 to negatively control cold tolerance via JA-activated signaling in rice 被引量:1
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作者 Yan Wang Yang Shen +6 位作者 Weifeng Dong Xiaoxi Cai Junkai Yang Yue Chen Bowei Jia Mingzhe Sun Xiaoli Sun 《The Crop Journal》 SCIE CSCD 2024年第5期1447-1458,共12页
Plant Homeo Domain(PHD)proteins are involved in diverse biological processes during plant growth.However,the regulation of PHD genes on rice cold stress response remains largely unknown.Here,we reported that PHD17 neg... Plant Homeo Domain(PHD)proteins are involved in diverse biological processes during plant growth.However,the regulation of PHD genes on rice cold stress response remains largely unknown.Here,we reported that PHD17 negatively regulated cold tolerance in rice seedlings as a cleavage target of miR1320.PHD17 expression was greatly induced by cold stress,and was down-regulated by miR1320 overexpression and up-regulated by miR1320 knockdown.Through 5'RACE and dual luciferase assays,we found that miR1320 targeted and cleaved the 3'UTR region of PHD17.PHD17 was a nuclearlocalized protein and acted as a transcriptional activator in yeast.PHD17 overexpression reduced cold tolerance of rice seedlings,while knockout of PHD17 increased cold tolerance,partially via the CBF cold signaling.By combining transcriptomic and physiological analyses,we demonstrated that PHD17 modulated ROS homeostasis and flavonoid accumulation under cold stress.K-means clustering analysis revealed that differentially expressed genes in PHD17 transgenic lines were significantly enriched in the jasmonic acid(JA)biosynthesis pathway,and expression of JA biosynthesis and signaling genes was verified to be affected by PHD17.Cold stress tests applied with MeJA or IBU(JA synthesis inhibitor)further suggested the involvement of PHD17 in JA-mediated cold signaling.Taken together,our results suggest that PHD17 acts downstream of miR1320 and negatively regulates cold tolerance of rice seedlings through JA-mediated signaling pathway. 展开更多
关键词 RICE cold tolerance PHD protein miR1320 JA signaling
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Endogenous serotonin induced by cold acclimation increases cold tolerance by reshaping the MEL/ROS/RNS redox network in Kandelia obovata
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作者 Junjian Li Huiyu Zhang +4 位作者 Danfei Yue Siyi Chen Yaxin Yin Chunfang Zheng Yan Chen 《Journal of Forestry Research》 SCIE EI CAS CSCD 2024年第6期73-85,共13页
Cold stress severely limits the distribution of mangrove species worldwide and it remains unclear how mangroves respond and adapt to cold temperatures.In this study,we investigated the effects of cold acclimation and/... Cold stress severely limits the distribution of mangrove species worldwide and it remains unclear how mangroves respond and adapt to cold temperatures.In this study,we investigated the effects of cold acclimation and/or inhibition of serotonin levels on reactive oxygen species(ROS),reactive nitrogen species(RNS),melatonin(MEL)and serotonin(SER)accumulation during cold stress in Kandelia obovata.Morphologic observation and param-eter analysis revealed that cold acclimation mitigated the photoinhibition of photosystem I(PSI)and photosystem II(PSII),maintained optimal ROS and RNS redox homeosta-sis,and increased the contents of SER and MEL in leaves.This suggests that cold acclimation reshapes the MEL/ROS/RNS redox network.In particular,the tryptophan/tryptamine/Ser/N-acetylserotonin/MER pathway was identi-fied as a branch of the MEL synthesis pathway.Inhibition of endogenous SER exacerbated damage caused by cold stress,indicating the crosstalk of SER synthesis and cold acclima-tion.In this study,we report a coordinated regulation of cold stress by a complex defense network in K.obovata. 展开更多
关键词 cold acclimation SEROTONIN MELATONIN cold stress MANGROVES
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Microstructure and Properties of Low-pressure Cold Sprayed Al-based Composite Coatings on Magnesium Alloy Surface
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作者 LIU Hongjin DING Minghui +1 位作者 WANG Haoxuan YAN Pengwei 《上海航天(中英文)》 CSCD 2022年第1期84-91,共8页
Pure Al and Al-30%Al_(2)O_(3)composite coatings are prepared on the surface of AZ31B magnesium alloy by low-pressure cold spraying.The morphology and structure of the coatings are analyzed by scanning electron microsc... Pure Al and Al-30%Al_(2)O_(3)composite coatings are prepared on the surface of AZ31B magnesium alloy by low-pressure cold spraying.The morphology and structure of the coatings are analyzed by scanning electron microscope (SEM),energy dispersive spectroscopy (EDS),and X-ray diffraction (XRD),and the effects of the addition of Al_(2)O_(3)on the microstructure of the Al-based coatings are discussed.The mechanical properties and corrosion resistance of the coatings are fully evaluated by the micro-hardness tester,electronic tensile machine,and electrochemical workstation.The results show that the coating structure is more uniform and denser,and the porosity is significantly reduced after the addition of Al_(2)O_(3).The interfaces between the coatings and the magnesium alloy substrate are distinct,and the coatings and the substrate are mechanically combined.Compared with the pure Al coating,the microhardness of the Al-Al_(2)O_(3)composite coating is increased to 61.1 HV_(0.2),and the bonding strength reaches above 53.1 MPa.The self-corrosion potential of the two coatings is higher than that of the magnesium alloy,and the self-corrosion current density is significantly lower than that of the magnesium alloy substrate.The Al-based coatings prepared by low-pressure cold spraying have high hardness,good bonding strength,and good corrosion resistance,which can be used for the repair and protection of magnesium alloy structural parts. 展开更多
关键词 AL Al-Al_(2)O_(3) low-pressure cold spray mechanical property corrosion resistance
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Effect of β-Glucan (Angel Yeast) Compared to a Placebo on Cold and Flu Incidence and Symptoms in an Adult Population—A Double Blind, Randomised Controlled Trial
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作者 David Briskey Haibo Zhang +1 位作者 Zhixian Chen Amanda Rao 《Food and Nutrition Sciences》 CAS 2024年第6期484-497,共14页
Background: 1-3, 1-6 β-glucan derived from Baker’s yeast (Saccharomyces cerevisiae) has been widely studied for its immune stimulatory capabilities and safety. Previous studies found β-glucan to have efficacy at re... Background: 1-3, 1-6 β-glucan derived from Baker’s yeast (Saccharomyces cerevisiae) has been widely studied for its immune stimulatory capabilities and safety. Previous studies found β-glucan to have efficacy at reducing incidence of URTIs as well as being a low risk for negative side effects. The current study aimed to examine the effects of yeast β-glucan (Angel Yeast) on cold and flu incidences and symptoms in healthy adults. Methods: Two hundred and thirty-one males and females aged 18 to 65 years old supplemented with either β-glucan or a placebo for 3-months. Participants completed a general health questionnaire every 4 weeks and in addition, if participants experienced any cold or flu symptoms, these were recorded daily (along with severity) until resolved or up to 2 weeks. Results: Supplementation with β-glucan reduced the self-reported severity of sore throats and improved sleep quality compared to the placebo group. Conclusions: Yeast β-glucan supplementation appears to be able to help reduce certain symptoms experienced during a cold or flu episode and is safe and well tolerated. 展开更多
关键词 BETA-GLUCAN cold FLU Baker’s Yeast
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The UDP-glycosyltransferase OsUGT706D2 positively regulates cold and submergence stress tolerance in rice
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作者 Qing Liu Lanlan Zhang +12 位作者 Shuwei Lyu Hang Yu Wenjie Huang Liqun Jiang Jing Zhang Bingrui Sun Xingxue Mao Pingli Chen Junlian Xing Wenfeng Chen Zhilan Fan Shijuan Yan Chen Li 《The Crop Journal》 SCIE CSCD 2024年第3期732-742,共11页
In a genome-wide association study,we identified a rice UDP-glycosyltransferase gene,OsUGT706D2,whose transcription was activated in response to cold and submergence stress and to exogenous abscisic acid(ABA).OsUGT706... In a genome-wide association study,we identified a rice UDP-glycosyltransferase gene,OsUGT706D2,whose transcription was activated in response to cold and submergence stress and to exogenous abscisic acid(ABA).OsUGT706D2 positively regulated the biosynthesis of tricin-4’-O-(syringyl alcohol)ether-7-O-glucoside at both the transcriptional and metabolic levels.OsUGT706D2 mediated cold and submergence tolerance by modulating the expression of stress-responsive genes as well as the abscisic acid(ABA)signaling pathway.Gain of function of OsUGT706D2 increased cold and submergence tolerance and loss of function of OsUGT706D2 reduced cold tolerance.ABA positively regulated OsUGT706D2-mediated cold tolerance but reduced submergence tolerance.These findings suggest the potential use of OsUGT706D2 for improving abiotic stress tolerance in rice. 展开更多
关键词 UDP-glycosyltransferase RICE cold tolerance Submergence tolerance ABA
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Boosting thermoelectric efficiency of Ag_(2)Se through cold sintering process with Ag nano-precipitate formation
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作者 Dejwikom Theprattanakorn Thanayut Kaewmaraya Supree Pinitsoontorn 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第12期2760-2769,共10页
Silver selenide(Ag_(2)Se)stands out as a promising thermoelectric(TE)material,particularly for applications near room temper-atures.This research presents a novel approach for the fabrication of bulk Ag_(2)Se samples ... Silver selenide(Ag_(2)Se)stands out as a promising thermoelectric(TE)material,particularly for applications near room temper-atures.This research presents a novel approach for the fabrication of bulk Ag_(2)Se samples at a relatively low temperature(170℃)using the cold sintering process(CSP)with AgNO_(3)solution as a transient liquid agent.The effect of AgNO_(3)addition during CSP on the micro-structure and TE properties was investigated.The results from phase,composition and microstructure analyses showed that the introduc-tion of AgNO_(3)solution induced the formation of Ag nano-precipitates within the Ag_(2)Se matrix.Although the nano-precipitates do not af-fect the phase and crystal structure of orthorhombicβ-Ag_(2)Se,they suppressed crystal growth,leading to reduced crystallite sizes.The samples containing Ag nano-precipitates also exhibited high porosity and low bulk density.Consequently,these effects contributed to sig-nificantly enhanced electrical conductivity and a slight decrease in the Seebeck coefficient when small Ag concentrations were incorpor-ated.This resulted in an improved average power factor from~1540μW·m^(−1)·K^(−2)for pure Ag_(2)Se to~1670μW·m^(−1)·K^(−2)for Ag_(2)Se with additional Ag precipitates.However,excessive Ag addition had a detrimental effect on the power factor.Furthermore,thermal conductiv-ity was effectively suppressed in Ag_(2)Se fabricated using AgNO_(3)-assisted CSP,attributed to enhanced phonon scattering at crystal inter-faces,pores,and Ag nano-precipitates.The highest figure-of-merit(zT)of 0.92 at 300 K was achieved for the Ag_(2)Se with 0.5wt%Ag dur-ing CSP fabrication,equivalent to>20%improvement compared to the controlled Ag_(2)Se without extra Ag solution.Thus,the process outlined in this study presents an effective strategy to tailor the microstructure of bulk Ag_(2)Se and enhance its TE performance at room temperature. 展开更多
关键词 THERMOELECTRIC silver selenide CHALCOGENIDE cold sintering process nano-precipitate
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Transcription factor CabHLH035 promotes cold resistance and homeostasis of reactive oxygen species in pepper
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作者 Huafeng Zhang Jiangbai Guo +7 位作者 Xiaoqing Chen Yunyun Zhou Yingping Pei Lang Chen Saeed ul Haq Mingke Zhang Haijun Gong Rugang Chen 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第3期823-836,共14页
Plant basic helix-loop-helix(bHLH)transcription factors(TFs)play central roles in various abiotic stresses.However,its role in plant cold resistance is largely unknown.Previously,we characterised CaNAC035 in pepper,wh... Plant basic helix-loop-helix(bHLH)transcription factors(TFs)play central roles in various abiotic stresses.However,its role in plant cold resistance is largely unknown.Previously,we characterised CaNAC035 in pepper,which positively regulates tolerance to cold,salt and drought stresses tolerance.Here,we identified CabHLH035,a CaNAC035-interacting protein in pepper.To explore its functions in cold stress tolerance,we silenced the gene in pepper via virus-induced gene silencing(VIGS)and overexpressed the gene in Arabidopsis.The results showed that CabHLH035 expression was induced by cold treatment,and silencing of CabHLH035 decreased cold stress tolerance.Conversely,overexpression of CabHLH035 in Arabidopsis increased cold stress tolerance.To investigate homologs genes of C-repeat binding factor(CBF)pathway proteins and reactive oxygen species(ROS)marker gene expression blocking by CabHLH035,we performed yeast one-hybrid(Y1H),dual luciferase and electrophoretic mobility shift assay experiments.The results showed that CabHLH035 bound to the region upstream of the CaCBF1A and CaAPX promoters.Additionally,CaCBF1A bound to the CaDHN4 promoter.Taken together,our results showed that CabHLH035 plays a crucial role in cold stress tolerance and its potential as a target for breeding cold-resistant crops.The findings provide a basis for studying the functions and regulatory network of cold stress tolerance in pepper. 展开更多
关键词 PEPPER CabHLH035 SILENCING Overexpression VIGS cold tolerance
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QTL mapping by GWAS and functional analysis of OsbZIP72 for cold tolerance at rice seedling stage
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作者 Yunsong Gu Haifeng Guo +15 位作者 Huahui Li Runbin Su Najeeb Ullah Khan Jin Li Shichen Han Weitong Zhao Wei Ye Shilei Gao Andong Zou Meng Zhang Xingming Sun Zhanying Zhang Hongliang Zhang Pingrong Yuan Jinjie Li Zichao Li 《The Crop Journal》 SCIE CSCD 2024年第6期1697-1708,共12页
Rice is a major crop susceptible to chilling stress.The identification of quantitative trait loci and genes for cold tolerance is crucial for the rice breeding.Of 30 quantitative-trait loci affecting seedling cold tol... Rice is a major crop susceptible to chilling stress.The identification of quantitative trait loci and genes for cold tolerance is crucial for the rice breeding.Of 30 quantitative-trait loci affecting seedling cold tolerance identified in a genome-wide association study of 540 rice accessions,OsbZIP72 was assigned as the causative gene for one,qCTS9.1.A single-nucleotide polymorphism in its promoter accounted for variation in expression between indica and japonica subspecies.The favorable haplotype of OsbZIP72 originated in wild rice and contributed to the expansion of japonica rice to colder habitats.OsbZIP72 positively regulates genes coding reactive oxygen species(ROS)-scavenging proteins and maintains intracellular ROS homeostasis.These findings not only enhanced our understanding of environmental adaptation but also provide novel genetic resources and potential targets for molecular design breeding for cold tolerance in rice. 展开更多
关键词 Rice GWAS cold tolerance OsbZIP72 ROS
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Miniature laser frequency stabilization module for cold atom sensing
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作者 Qingzhe Gou Ning Wei +4 位作者 Haixu Tao Kang Cheng Yongtao Fan Jiajing He Jun Wang 《Chinese Optics Letters》 SCIE EI CAS CSCD 2024年第10期65-69,共5页
In this study,a miniature laser frequency stabilization module for cold atom sensing applications was designed and realized.The module can lock the laser frequency to the saturated absorption spectrum of the D2 line o... In this study,a miniature laser frequency stabilization module for cold atom sensing applications was designed and realized.The module can lock the laser frequency to the saturated absorption spectrum of the D2 line of rubidium(Rb)atoms using the frequency modulation spectroscopy method.The module core is a tiny Rb cell,co-packaged with the saturation absorption optical setup,temperature control circuit,and detection circuit through a joint optical-mechanical-electrical design for a total volume of only 40 mm×15 mm×30 mm.The frequency fluctuation of the 780 nm laser after frequency stabilization by the module was within 1 MHz over 1000 s,which is adequate for magneto-optical trap experiments.The results verify the feasibility of the module as a frequency reference and provide a light source for portable cold atom sensing devices. 展开更多
关键词 frequency stabilization MINIATURIZATION cold atoms saturation absorption spectrum
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Melatonin mitigates cold-induced damage to pepper seedlings by promoting redox homeostasis and regulating antioxidant profiling
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作者 Muhammad Ahsan Altaf Yuanyuan Hao +9 位作者 Huangying Shu Weiheng Jin Chuhao Chen Lin Li Yu Zhang Muhammad Ali Mumtaz Huizhen Fu Shanhan Cheng Guopeng Zhu Zhiwei Wang 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期532-544,共13页
This study assessed the influence of exogenous ME in the mitigation of cold damage in pepper seedlings. Melatonin(ME) is a dynamic molecule that helps plants cope with stress in several ways. Cold stress(CS) is one of... This study assessed the influence of exogenous ME in the mitigation of cold damage in pepper seedlings. Melatonin(ME) is a dynamic molecule that helps plants cope with stress in several ways. Cold stress(CS) is one of the most important environmental factors that restrict plant growth and yield. Pepper(Capsicum annuum L.) is a valuable commercial crop, highly sensitive to CS. Thus, identifying an efficient strategy to mitigate cold damage is critical for long-term pepper production. For this purpose, the roots of pepper seedlings were pretreated with ME(5 μmol · L^(-1)) and exposed to CS for 7 d. The results indicated that CS suppressed pepper growth, hampered photosynthetic capacity, and damaged root architecture in pepper plants. In contrast, the production of reactive oxygen species(ROS), malondialdehyde(MDA), electrolyte leakage(EL), proline, and soluble sugars were enhanced in plants under CS. ME(5 μmol · L^(-1)) pretreatment reduced the negative effects of CS by recovering plant growth, root traits, gas exchange elements, and pigment molecules compared to CS control treatment. Furthermore, ME application efficiently reduced oxidative stress markers [hydrogen peroxide(H_(2)O_(2)), superoxide ion(O_(2)^(·-)), EL, and MDA] while increasing proline and soluble sugar content in pepper leaves. ME application combined with CS further increased antioxidant enzymes and related gene expression. Collectively, our results confirmed the mitigating potential of ME supplementation for CS by maintaining pepper seedling growth,improving the photosynthesis apparatus, regulating pigments, and osmolyte content. 展开更多
关键词 PEPPER MELATONIN cold stress Antioxidant enzyme Root trait
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Supply of Dissolved Organic Carbon from the Cold Seeps-Hydrothermal System and Its Impact on the Deep-Sea Carbon Cycle:An Overview
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作者 ZHANG Xianrong SUN Zhilei +3 位作者 WANG Libo ZHANG Xilin ZHAI Bin XU Cuiling 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第6期1469-1480,共12页
Dissolved carbon(dissolved organic carbon and dissolved inorganic carbon)is the major component of the ocean carbon cycle,representing one of the largest carbon pools on Earth.Cold seeps and hydrothermal systems serve... Dissolved carbon(dissolved organic carbon and dissolved inorganic carbon)is the major component of the ocean carbon cycle,representing one of the largest carbon pools on Earth.Cold seeps and hydrothermal systems serve as the two main windows for the material and energy recycling exchange between the lithosphere and outer spheres(biosphere,hydrosphere and atmosphere).However,recent studies have found that the dynamic activities of fluids in these two extreme systems are a crucial source of‘new'carbon in the deep ocean.These carbon sources may become vital contributors to carbon and energy in marine ecosystems,which affect the global deep-sea carbon budget,and the marine ecosystems as well.In this review,we summarize the sources and formation mechanisms of dissolved carbon in the seep fluids from the cold seeps and hydrothermal vents,the contribution of methane oxidation to dissolved carbon,and the characteristics of the carbon isotope composition in the fluid.Furthermore,we analyze and discuss the influence of carbon discharged from seabed on the seawater carbon cycle by comparing and contrasting these two extreme environments.The research may assist in promoting a deeper understanding of the carbon cycle and material interaction in the ocean,particularly further carbon cycle research in the back-arc basin where cold seeps and hydrothermal vents commonly prevail. 展开更多
关键词 dissolved carbon cold seep hydrothermal vent FLUID carbon cycle
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Dietary fat supplementation relieves cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis in pigs
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作者 Wei He Xinyu Liu +4 位作者 Ye Feng Hongwei Ding Haoyang Sun Zhongyu Li Baoming Shi 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第4期1669-1687,共19页
Background Cold stress has negative effects on the growth and health of mammals, and has become a factor restricting livestock development at high latitudes and on plateaus. The gut-liver axis is central to energy met... Background Cold stress has negative effects on the growth and health of mammals, and has become a factor restricting livestock development at high latitudes and on plateaus. The gut-liver axis is central to energy metabolism, and the mechanisms by which it regulates host energy metabolism at cold temperatures have rarely been illustrated. In this study, we evaluated the status of glycolipid metabolism and oxidative stress in pigs based on the gut-liver axis and propose that AMP-activated protein kinase(AMPK) is a key target for alleviating energy stress at cold temperatures by dietary fat supplementation.Results Dietary fat supplementation alleviated the negative effects of cold temperatures on growth performance and digestive enzymes, while hormonal homeostasis was also restored. Moreover, cold temperature exposure increased glucose transport in the jejunum. In contrast, we observed abnormalities in lipid metabolism, which was characterized by the accumulation of bile acids in the ileum and plasma. In addition, the results of the ileal metabolomic analysis were consistent with the energy metabolism measurements in the jejunum, and dietary fat supplementation increased the activity of the mitochondrial respiratory chain and lipid metabolism. As the central nexus of energy metabolism, the state of glycolipid metabolism and oxidative stress in the liver are inconsistent with that in the small intestine. Specifically, we found that cold temperature exposure increased glucose transport in the liver, which fully validates the idea that hormones can act on the liver to regulate glucose output. Additionally, dietary fat supplementation inhibited glucose transport and glycolysis, but increased gluconeogenesis, bile acid cycling, and lipid metabolism. Sustained activation of AMPK, which an energy receptor and regulator, leads to oxidative stress and apoptosis in the liver;dietary fat supplementation alleviates energy stress by reducing AMPK phosphorylation.Conclusions Cold stress reduced the growth performance and aggravated glycolipid metabolism disorders and oxidative stress damage in pigs. Dietary fat supplementation improved growth performance and alleviated cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis. In this study, we high-light the importance of AMPK in dietary fat supplementation-mediated alleviation of host energy stress in response to environmental changes. 展开更多
关键词 Apoptosis cold temperature Energy stress FAT Glucolipid metabolism Mitochondrial homeostasis
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